Center For Regenerative Medicine ME-DENT is a cutting edge medical clinic that uses advanced regenerative technologies to treat chronic diseases for which there are inadequate standard therapies. The using of autologous tissues repair cells as they have no ethical or moral issues and pose no possibility for rejection since they come from the patient's own body.

1. Autologous Bone Marrow Derived Mononuclear Cells
combined with Bone Replacement Materials*
in Craniofacial Tissues Regeneration
* Bio-Oss®, Ossceram nano®, Cerabone®-Granulate, OSferion®
“ME-DENT”
The Center for Regenerative Medicine
Rovinj, Croatia

2. Bone marrow contains cells, called Autologous Bone Marrow Derived
Mononuclear Cells that have the potential to induce bone tissue when they are
provided the biological direction to do so. Accordingly, bone marrow has been used
in different ways to augment new bone growth in a variety of medical procedures
such as repair of severe fractures, and void filling of large bone defects. Inducing
new bone tissue in jawbones with minimal residual ridge or basal bone by bone
grafting has become an accepted procedure to increase bone thickness enough to
allow placement of dental implants to replace lost teeth.

3. In our procedures we used only
bone marrow autologous (patient’s
own) cells.
This way is absolutely safe for the
patient because there are not any
immunological conflicts.
Moreover, in contrast to majority of clinical researchers we have used a mononuclear cells
fraction of the bone marrow because an associated work of stem cells and other cells
contained in the fraction gives better expected result.

4. Our Center has introduced a new technology "Autologous bone marrow derived mononuclear
cells combined with Bone Replacement Materials (BRM) in Advanced Maxillary and
Mandibular Atrophy" - a safe effective method for replacing missing bone and adding mass to
existing bone for dental implants.
Mixing of Bone Marrow Mononuclear Cells
with Bone Replacement Materials
Bone Marrow Mononuclear Cells
Bone Replacement Materials

5. The model of natural bone regeneration by bone marrow derived mononuclear cells
combined with BRM*
1 2 3
1. Clot stabilization facilitated by Bone Marrow Mononuclear Cells + BRM
interconnecting macro and micropores.
2. Revascularization, migration of osteoblasts (purple) and in-growth of woven
bone (yellow) is enhanced by Bone Marrow Mononuclear Cells + BRM
scaffolding.
3. Lamellar bone and Bone Marrow Mononuclear Cells + BRM are successfully
integrated after approximately six months. BRM is included in the natural
physiologic remodeling process (osteoclasts - Blue).

6. Introduction
Replacing missing bone or adding mass to existing bone is
often essential to the success of a dental implant.
A large variety of graft materials have been used for maxillary
and mandibular atrophy.
To date there has been no graft material which can be
regarded as completely satisfactory.
Our experience with autologous bone marrow derived
mononuclear cells combined with Bone Replacement
Materials (BRM) for augmentation of the extremely atrophied
maxilla and mandible is presented.

7. Advanced Diagnostic for Maxillary and Mandibular Atrophy
Planmeca ProMax 3D concept is an
intelligent and multipurpose X-ray
unit series designed to obtain
complete information on patient
anatomy.
The unit provide digital panoramic,
cephalometric, 3D CBVT* imaging,
and 3D photo, as well as advanced
imaging software tools to comply
with every possible need in dental
radiology.
* Cone Beam Volumetric Tomography

8. Autologous Bone Marrow from patients used as a source for
Mononuclear Cells.
Bone Marrow Mononuclear separated by
Cell Separation System SEPAX S-100 (Biosafe Group SA, Switzerland).
Autologus Bone Marrow
in plastic bag
Bone Marrow exfusion Bone Marrow Mononuclear Bone Marrow
Cells separation Mononuclear Cells

9. Date: 10 October 2009
Patient : D. P., 56 y. o., male
Diagnose: Advanced Maxillary and Mandibular Atrophy, bone resorption,
parodontosis, gum recession.

11. Window preparation
and sinus membrane
elevation

12. Implant placement

13. Sinus filled by BRM
combined with
autologous bone
marrow derived
mononuclear cells

14. Gum stitching

15. Before
Healing period
in 1 month.
Formation of
new bone
can be observed.
The augmentation material is fully
integrated into the hard tissue.

16. Before
Healing period
in 5 months.
Complete
osteoconductive
integration of
the bone subtitute
material in the
mature bone is
achieved.

17. Healing period
in 5 months.
Complete
osteoconductive
integration of
the bone subtitute
material in the mature
bone is achieved.

18. Before
Healing period In 7 months

19. Date 29 January 2010
Patient : S. L., 46 y. o., female
Diagnose: Advanced Maxillary and Mandibular Atrophy,
bone resorption, parodontosis, gum recession.

20. Advanced Maxillary Atrophy

21. Window preparation and sinus membrane elevation

23. JASON® MEMBRANE
(pericardium collagen membrane)

24. Gum stitching

25. Healing periods
29.01.2010
05.08.2010
10.08.2010

26. Advanced Maxillary Atrophy 30.01.2011 - in 1 year

27. Date of procedure: 25 November 2010
Diagnose: Advanced Maxillary Atrophy, bone resorption, Parodontosis,
gum recession.
19.07.2011
08.06.2011 27