biosensors, types of biosensors

1. BIOSENSORS
BY,
A. POOJA SHUKLA
M. Tech (I yr)
1821310006
SRM UNIVERSITY

2. WHAT IS SENSOR…??
A sensor is a converter that
measures a physical quantity
and converts it into a signal
which can be read by an
observer or by an instrument.

3.  Nanosensors are any
biological, chemical, or surgical sensory
points used to convey information about
nanoparticles to the macroscopic world.
 medicinal purposes
 nanoproducts, such as computer chips
that work at the nanoscale and
nanorobots.
WHAT IS NANO - SENSOR…??

4. WHAT IS BIO - SENSOR…??
A biosensor is an analytical device, used for the
detection of an analyte, that combines a biological
component with a physicochemical detector.

5. Father of the Biosensor
Professor Leland C Clark Jnr
1918–2005
 A device incorporating a biological sensing
element either intimately connected to or
integrated within a transducer.
 Recognition based on affinity between
complementary structures like:
 enzyme-substrate, antibody-antigen
and receptor-hormone complex.
 Selectivity and specificity depend on
biological recognition systems connected to
a suitable transducer.
BIO - SENSOR…??

6.  It is an analytical device which converts a biological
response into an electrical signal.
 It detects, records, and transmits information regarding a
physiological change or process.
 It determines the presence and concentration of a specific
substance in any test solution.
BIO - SENSOR…??

7. Basic priciple of biosensor involved in three element :-
 First biological recognization element which highly specific towards the
biological material analytes produces.
 Second transducesrs detect and transduces signal from biological target -
receptor molecule to electrical signal which is due to reaction occur.
 Third after transduction sinal from biological to electrical signal where its
amplification is necessary and takes place and read out in detector after
processing the values are displayed for monitor and controlling the system .
BASIC PRINCIPLE OF BIOSENSOR

8. BASIC PRINCIPLE OF BIOSENSOR

9.  The biological material is immobilized and a contact is made between the
immobilized biological material and the transducer
 The analyte binds to the biological material to form a bound analyte which in
turn produces the electronic response that can be measured.
 Sometimes the analyte is converted to a product which could be associated
with the release of heat, gas (oxygen), electrons or hydrogen ions. The
transducer then converts the product linked changes into electrical signals
which can be amplified and measured
BASIC PRINCIPLE OF BIOSENSOR

10. Detector
COMPONENTS OF BIOSENSOR

11. Microorganism
Tissue
Cell
Organelle
Nucleic Acid
Enzyme
Enzyme Component
Receptor
Antibody
The component used to bind the target molecule.
Must be highly specific, stable under storage conditions, and immobilized.
1st COMPONENT – BIOLOGICAL
ELEMENT

12.  Function
To interact specifically with a target compound i.e. the
compound to be detected.
 It must be capable of detecting the presence of a target
compound in the test solution.
 The ability of a bio-element to interact specifically with target
compound (specificity) is the basis for biosensor.
1st COMPONENT – BIOLOGICAL
ELEMENT

13. Acts as an interface, measuring the physical change that occurs with the reaction at
the bioreceptor then transforming that energy into measurable electrical output.
2nd COMPONENT –
PHYSIOCHEMICAL TRANSDUCER

14. Signals from the transducer are passed
to a microprocessor where they are
amplified and analyzed.
The data is then converted to
concentration units and transferred to a
display or/and data storage device.
3rd COMPONENT – DETECTOR

15. PRINCIPLE OF DETECTION
PIEZOELECTRIC Measures change in mass
ELECTRO-MECHANICAL Measures change in electric
distribution
OPTICAL Measures change in light
intensity
CALORIMETRIC Measures change in heat

16. a- Bio-element
b- Transducer
Figure. Schematic Diagram of Biosensor c- Amplifier
d- Processor
e- Display
WORKING OF BIOSENSOR

17.  Biosensors basically involve the quantitative analysis of
various substances by converting their biological actions
into measurable signals.
 Generally the performance of the biosensors is mostly
dependent on the specificity and sensitivity of the
biological reaction, besides the stability of the enzyme.
HOW DOES THEY WORK…??

18. Analyte
Sample
handling/preparation
Detection
Signal
Analysis
Response
HOW DOES THEY WORK…??

19.  The output signal must be relevant to measurement
environment.
 The functional surface must be compatible with the
transducer.
 High specificity and selectivity (low interference).
 Sufficient sensitivity and resolution .
IDEAL BIOSENSOR

20.  Sufficient accuracy and repeatability
 Sufficient speed of response
 Sufficient dynamic range.
 Insensitivity to environmental interference or their
effects must be compensated
IDEAL BIOSENSOR

21. 1. LINEARITY - Linearity of the sensor should be high for the detection of
high substrate concentration.
2. SENSITIVITY - Value of the electrode response per substrate
concentration.
3. SELECTIVITY - Chemicals Interference must be minimised for
obtaining the correct result.
4. RESPONSE TIME - Time necessary for having 95% of the response.
BASIC CHARACTERISTICS OF
BIOSENSOR

22. BIOSENSOR TECHNOLOGY

24.  Based on bioreceptors
Enzyme biosensor
Microbial biosensor
Affinity biosensor
 Based on transducer
Potentiometric
Amperometric
conductometric
Optical
Acoustic or piezoelectric etc.
TYPES OF BIOSENSOR

25. • Colorimetric for color: Measure
change in light adsorption as reactants
are converted to products.
• Photometric for light intensity:
Photon output for a luminescent or
fluorescent process can be detected
with photomultiplier tubes or
photodiode systems.
OPTICAL BIOSENSORS

26. If the enzyme catalyzed reaction is exothermic, two thermistors
may be used to measure the difference in resistance between
reactant and product and hence the analyte concentration.
CALORIMETRIC BIOSENSORS

27. For voltage: Change in distribution of charge is
detected using ion-selective electrodes, such as pH-
meters.
POTENTIOMETRIC
BIOSENSORS

28. The change in frequency is proportional to the mass of
absorbed material.
Piezo-electric devices use gold to detect the specific angle at
which electron waves are emitted when the substance is
exposed to laser light or crystals, such as quartz, which vibrate
under the influence of an electric field.
PIEZO - ELECTRIC
BIOSENSORS

29.  Principle
Many chemical reactions produce or consume ions or electrons which
in turn cause some change in the electrical properties of the solution
which can be sensed out and used as measuring parameter.
 Classification
(1) Amperometric biosensor
(2) Conductimetric biosensor
(3) Potentiometric biosensor
ELECTROCHEMICAL
BIOSENSORS

30. •Amperometric for applied current: Movement of e- in redox
reactions detected when a potential is applied between two
electrodes.
•Potentiometric for voltage: Change in distribution of charge is
detected using ion-selective electrodes, such as pH-meters.
•Conductimetric for impedance
ELECTROCHEMICAL
BIOSENSORS

31.  Measuring parameter : Electric current
 Based on oxidase enzymes that generate H2O2 and consume
oxygen.
 Formation of H2O2 can be detected by the help of Pt-electrode.
AMPEROMETRIC BIOSENSORS

32.  Glucose reacts with glucose
oxidase(GOD) to form gluconic acid.
Two electrons & two protons are also
produced.
 Glucose mediator reacts with
surrounding oxygen to form H2O2 and
GOD.
 Now this GOD can reacts with more
glucose.
 Higher the glucose content, higher the
oxygen consumption.
 Glucose content can be detected by Pt-
electrode.
GLUCOSE BIOSENSORS

33.  Steps involved in electrochemical DNA
hybridization biosensors:
 Formation of the DNA recognition layer
 Actual hybridization event
 Transformation of the hybridization event into an
electrical signal
ELECTROCHEMICAL DNA
BIOSENSORS

34. Motivated by the application to clinical diagnosis and
genome mutation detection
 Electrodes
 Chips
 Crystals
DNA BIOSENSORS
TYPES

35. Pregnancy test - Detects the hCG protein in urine.
EXAMPLE OF BIOSENSOR

36. Glucose monitoring device (for diabetes patients)
Monitors the glucose level in the blood.
EXAMPLE OF BIOSENSOR

37. BIOSENSORS AT
NANOSCALE

38. WHAT IS NANO BIOSENSOR…??
Comparison of nano-rangeNano - very small
Includes many fields
Its the Miniatured
Biology
Nanotechnology-
manipulating matter at
nanoscale.
 BIO-SENSOR AT NANOSCALE

39. Nanoparticles have novel property.
Integration of material science, molecular engineering,
chemistry and biotechnology.
Improve the sensitivity and specificity of biomolecule
detection
Efficient biomolecular recognition, pathogenic diagnosis
and environment monitoring.
WHY NANO BIOSENSOR…??

40. PROPERTIES PARTICLES USED
 Gold nanoparticle
 Carbon Nanotubes
 Magnetic particles
 Quantum dots
Unique
• physical
• chemical,
• mechanical,
• magnetic and
• optical properties,
• markedly enhance the sensitivity
and specificity of detection.
NANOPARTICLES…??

41.  Allows the placement of small structures with
precision, simplicity and low cost.
 Small particles high efficiency
 Leads to economic growth
 Enhances national security
 Improves the quality of life
 Job oppurtunities
NEED FOR NANOPARTICLES…??

42. Material Medical
•Stronger •End of Illnesses (i.e. cancer,
heart disease)
•Lighter •Universal Immunity (i.e. aids,
flu)
•Cheaper •Body Sculpting (i.e. change
your appearance)
•Durable
•Precise
ADVANTAGE OF USING
NANOPARTICLES…??

43. Dr. Michael Strano at the University of Illinois, "We have developed molecular
sheaths around the nanotube that respond to a particular chemical and modulate
the nanotube's optical properties."
CURRENT RESEARCH
SPOT-NOSED Project: A layer of olfactory proteins on a nanoelectrode could
react with low-concentration odorants. This technology could be used by
doctors to diagnose diseases at earlier stages.

44. Nanosphere lithography (NSL) derived triangular Ag nanoparticles were used
to detect streptavidin down to one picomolar concentrations.
CURRENT RESEARCH
The School of Biomedical Engineering has developed an anti-body based
piezoelectric nanobiosensor to be used for anthrax, HIV hepatitis detection.

45.  Food Analysis
 Study of biomolecules and their interaction
 Drug Development
 Crime detection
 Medical diagnosis (both clinical and laboratory use)
 Environmental field monitoring
 Quality control
 Industrial Process Control
 Detection systems for biological warfare agents
 Manufacturing of pharmaceuticals and replacement organs
APPLICATIONS OF BIOSENSORS

46. NANO-NOSE:
 Food quality check using nanoparticles.
 Detection of various protein.
 For detection of diseases.
 Various proteins are sensed out in body fluids.
 Advantage:
 Highly sensitive
 96% accuracy
 Quick detection
APPLICATIONS AT NANOSCALE

47. Biological Applications
DNA Sensors: Genetic monitoring, disease diagnosis
Immunosensors: HIV, Hepatitis, other viral diseases drug
testing, environmental monitoring…
CURRENT APPLICATIONS

48. Biological Application
Cell-based Sensors: functional sensors, drug testing…
Point-of-care sensors:
blood, urine, electrolytes, gases, steroids,drugs, hormones,
proteins, other…
CURRENT APPLICATIONS

49. Biological Applications
Bacteria Sensors: (E-coli, streptococcus, other): food
industry,medicine, environmental, other.
Enzyme sensors: diabetics, drug testing, other.
CURRENT APPLICATIONS

50.  Health and safety issues
 Nanoparticles can cause serious illness or damage human body.
 Untraceable destructive weapons of mass destruction.
 Social & Political issues
 Advisability of increasing scope of the technology creates political
dilemma
 Creates social strife through increasing wealth gap
 Loss of jobs (in manufacturing, farming, etc)
 Carbon Nanotubes could cause infection of lungs
 Oil & Diamonds could become worthless
DISADVANTAGES

51.  Examples of Future Use of NT
 Electronic Paper
 Nokia Morph
 Contact Lens
FUTURE OF
NANOBIOTECHNOLOGY

52. FUTURE OF
NANOBIOTECHNOLOGY

53. THANK YOU…!!