TIssue culture

1. Tissue Culture Fun
Fruit
Nutrition
The Guide
Feb – 2012
Biotechnology
By:
Afida Zahara Adzkiya
Alifah Farah Gusmaniar
Indah Puspita Aziz
Ridha Astri Indryana
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

2. Fun
Fruit
Nutrition Definition of tissue culture
Tissue culture is an
activity to cultivate a tissue of plant
vegetatively to be a plant that has
similar property to its parent in a
short time.
Plants can be multiplied
using this technique as plants have
a totipotency ability, meaning that
from a tissue they can grow and
develop into new individuals.
Tissue culture is used to solve food
shortage problems.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

3. Fun
Fruit Information Layout Slide with Table
Nutrition
Tissue culture was found and well
known by F.C Steward, a plant physiologist
from United States. Who did his research in
1989. Steward try to make tissue culture of
carrot and he is success.
Tissue culture well because the
work of Muer, Hildebrant, and Riker in
1954.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

4. Fun
Fruit The Various Uses of Tissue Culture
Nutrition
• Obtaining a lot of germs in a
short time with similar
property to the parent.
• Making plants with
properties we want.
• Tissue culture is
economical from aspect of
time , space, and energy.
• Produce plants free from
virus and disease.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

5. The Procedure of Tissue Culture Technique
Cultivating tissue in
Preparing growth
Preparing tissue liquid growth
medium
medium
Moving plant whose
Cultivating plant in Cultivating plant in root bud and leaf bud
actual land pot containing soil have grown to another
solid growth medium
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

6. 1
Preparing
growth medium.
Substance that must exist in
growth medium consist of
minerals (macro element, such
as C, H, O, N and micro element
such as Zn, Mo), amino acid,
vitamin, sugar, and hormone in
certain amount.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

7. 2
Preparing tissue.
The tip part of a young shoot, such as root and
leaf tips, are cut into 1-1.5 mm³. Next, the tissue
is sterilized using 5% hypochlorite calcium and is
washed off several times with sterile aquadest.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

8. 3
Cultivating tissue (explants) in
liquid growth medium.
Explants are placed in a mixer table (shaker) that shakes at 60-
70 times per minute. The mixing is performed for 6 hours a day
for 1.5 – 2 months . Shaking is meant for faster distribution of
substance and food absorption as well as process of gas
exchange. In about 2 months, callus will grow in this liquid
medium.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

9. 4
Moving plant
whose root bud
and leaf bud have
grown to another
solid growth
medium.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

10. 5
Cultivating plant in
pot containing soil.
The plants whose rot bud and leaf bud
have grown are removed to another
solid medium to be separated, so
bigger plants can grow.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

11. 6
Cultivating plant in
actual land.
Before being cultivated in actual land, the
plant from solid medium is moved or
cultivated first in a pot that contains soil that
has been given fertilizer. If the condition of
plant has been strong, it can be moved to
actual land.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

12. Apakah objek yang dikultur oleh pempopuler tekhnik
kultur jaringan (Muer, Hildebrant, and Riker) sama
dengan objek yang dikultur oleh F. C. Steward?
Tidak sama.
Hildebrant berhasil mengembangkan tekhnik kultur jaringan pada sel-sel rambut dari
jaringan mesofil daun tanaman monokotil.
Muer berhasil menumbuhkan tanaman lengkap dari kultur sel tunggal.
Kalau Riker kami belum mengetahui apa objek yang dikulturnya, namun pada
logikanya itu pasti berbeda dengan objek yang dikultur oleh F. C. Steward.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.

13. Question from Sabila:
“What kind of plant that usually used for
tissue culture?”
Pada dasarnya semua jenid tumbuhan dapat dikultur, karena semua tumbuhan
memiliki sifat totipotensi di jaringan meristem (ujung akar dan batang). Namun
tumbuhan yang biasanya dikultur adalah:
-Yang pertumbuhannya lambat atau susah
-Memiliki nilai jual yang tinggi
-Banyak bergunan bagi masyarakat
-Contohnya adalah tanaman anggrek, jati dan tebu.
1987 Street Ave City, State
Balanced Nutrition Coming to Fruition www.funFruit.com
60623, US.