The document outlines a student project to design an ATP biosensor using firefly materials. It begins with an introduction to biosensors and their uses. The students then conducted experiments showing that firefly extracts produce light when ATP and buffer are added. Building on this, their project was to design an ATP biosensor. They created test tubes containing firefly materials with and without ATP that correctly detected its presence through luminescence only in the tube with ATP. The document concludes that they successfully created an ATP biosensor using firefly materials and phosphate buffer.

1. AL-BAIRAQ
I AM DISCOVERING MATERIALS
BIOSENSORS
Challengers7
Mohamed El deeb
Hamad a altamimi
Ahmed h al-abdulgani

2. OUTLINE
• Introduction
• Investigating Biological Molecules and
Bioluminescence
• Investigating enzymes and indicator
molecules
• Making a peroxide biosensor
• Testing a cholesterol biosensor
• Design Project (ATP Biosensor)
• Product Idea

3. INTRODUCTION
Biosensors
What are sensors ?
Nose! Tongue! Ears! Eyes! Fingers!
Sensors can give qualitative or quantitative analysis.

4. Sensors can be divided into
three types
physical sensors chemical sensors
biosensors

5. What is a Biosensors ?
Biosensors are which are of
providing either qualitative or
quantitative results.
The biological sensing element has
to be connected to a
transducer so that a visually
observable response occurs.
Biosensors function by coupling a
biological sensing element with a
detector system using a transducer.

6. Where are Biosensors Being Used ?
Healthcare (glucose, artificial pancreas) Food and drink production and analysis
Pollution control and monitoring

8. Objective
To see if the whole fireflies, whole firefly tail lanterns and firefly tail lantern
pieces will give off light after adding the buffer and ATP
Compare each of three firefly samples with the control
Notice which samples will give off light

9. Procedure
1. Label 4 tubes A,B,C and D
1- Two whole fireflies for 1st tube (W)
2- Two whole fireflies tail lanterns for 2nd tube (T)
3- Two firefly tail lantern pieces for 3rd tube (P)
4- last tube for control sample we (C) don’t contain firefly materials
Add 2 mL phosphate buffer and 0.1 mL ATP for each tube
Test the tubes in darken room and notice which tube will give off light

10. Results
Tube A,B and C will give off light
Tube D won’t give off light
The purpose of the control is to demonstrate that the firefly materials,
not the buffer or the ATP, are responsible for the luminescence

11. Conclusion
The firefly can give off light again if we add buffer and ATP we reactive it again
The reason of the luminescence is the firefly materials not the buffer and the ATP
The firefly materials give signal if we add buffer and ATP
We can use the firefly materials to search for the ATP
( ATP sensor)

12. summary
Firefly give signal when we add phosphate buffer and ATP
We can use Firefly materials as ATP sensor
firefly tail lantern pieces will give off stronger light than the whole
firefly tail lanterns

13. INVESTIGATING ENZYMES AND
INDICATOR MOLECULES
Activity (2)

14. Prepare 3ml of a 1% peroxide solution . .
Label 4 tubes (A.B.C.D)and follow the table
Prepare 0.1ml peroxidase darken the room .
Record all observations.
Procedure

15. Test Tube Peroxide Indicator peroxidase Type of
signal
A 0.1ml 1%
H2O2
Tip-full of
luminol
peroxidase Light
blue
B 0.1ml 1%
H2O2
Tip-full of sodium
luminol
peroxidase Light
Faint blue
C 0.1ml 1%
H2O2
0.2ml 4CN
solution .
peroxidase Purple
color
D 0.1ml 1%
H2O2
0.2ml TMB
solution .
peroxidase Yellow
color
Results

16. Light . Blue signal and we use Tip-full of
luminal indicator .
Light . Faint blue signal . And we use Tip-
full of sodium luminal indicator .
Using 4CN solution as indicator . Give us
purple color signal .
We have yellow color signal . When we use
TMB solution as indicator .
Conclusion

17. Activity (3)

18. Objective

19. Concentration of peroxide Observation of sensor strip
0.0003% 0cm
0.00075% 0.5cm
0.003% 1cm
0.0075% 2cm
0.025% 2.5cm
0.075 2.75cm
0.3 3cm
1% 3.5cm

21. The increasing of peroxide concentration , increase
the sensor strip observation .
The highest cone of peroxide = 1% Given us 3.5 cm
sensor strip .
Conclusion

22. TESTING A CHOLESTEROL
BIOSENSOR
Activity (4)

23. Set up 6 tubes A-F
Set up a control (Tube A and 0.2ml 10% trition )
And the cholesterol sample
And the enzyme solution
And 2ml TMP indicator solution (to add)
Procedure

24. Test tube Cholesterol cone
1 0.5 F
1:2 0.25 E
1:4 0.125 D
1:8 0.0625 C
1:16 0.3125 B
0 0 A
Concentration of the Cholesterol
Cholesterol + O2
Cholesterol
oxidase
Oxidized form of
cholesterol +
H2O2

25. Tube (1-F) represent the high concentration of cholesterol
Tube (B- represent 1:16 ratio) consider the lowest
concentration of cholesterol .
Conclusion

26. ATP BIOSENSOR

27. Important of ATP
it gives the cells energy to perform the tasks it needs to do to keep the body moving.
It makes the muscles of the body move.
Without it the cells would die and therefore so would the body.
ATP is important because the energy that is released, when its phosphate bonds are
hydrolyzed, is the energy that is used to drive endergonic reactions in cells.

28. Objective
Designing a Unique ATP
Biosensor
Designing ATP Biosensors Using
Luciferase Enzyme
Use the firefly materials and
phosphate buffer to design ATP

29. Procedure
Gather these materials
1- 2 tubes and Label them A and B
2- 4 firefly tail lantern pieces
3- 4 mL phosphate buffer
4- 0.1 mL ATP
A B
2 firefly tail lantern pieces 2 firefly tail lantern pieces
2 mL phosphate buffer 2 mL phosphate buffer
0.1 mL ATP No ATP
Check the tubes and darken room

30. Results
A B
luminescence No luminescence

31. Conclusion
We Can make an ATP Biosensor by using the firefly materials
and phosphate buffer
Tube A give off light because it’s contain ATP
Tube B didn’t give off light because it doesn’t contain ATP

32. INTRODUCTION AND RATIONALE
we choose this project because we got this idea from activity (1)
Investigating Biological Molecules and Bioluminescence and the ATP is
important searching for the ATP with this way will help the scientists to
know if there is ATP or no ATP and that way won’t take Time As they can
do it very fast and this is new idea and now one think of it before and this
will help the scientists in Qatar.
objectives : Design an ATP biosensor

33. we expected that answer of your research question will be that the ATP
and the firefly materials will give off light so that will be ATP biosensor
according to out experience with Albairaq workshops and the scientific
background and the researches related to our project.

34. OUR PROJECT STORY
We got the project idea from al-biraq activities and we work on it to get it
better and make new Biosensor . Al-biraq activities were very useful and it
helped us to get the idea . We used the internet to get more information
about the ATP and how can we get that biosensor better . Our project story
start from al-biraq activities and the Biosensor Module

35. PRODUCT IDEA
RESULTS
We used our product to search for
the ATP and we designed an ATP
Biosensor we found out that

36. RECOMMENDATIONS
Using ATP biosensor is very easy and it’s very accuracy
If anyone want to do work on this research I advice him
to try to get the concentration of the ATP because we
couldn’t get it

37. CONCLUSIONS
(OVERALL)
- The firefly can give off light again if we add buffer and ATP we reactive it
again
- The reason of the luminescence is the firefly materials not the buffer and
the ATP
- We can use the firefly materials to search for the ATP
- We Can make an ATP Biosensor by using the firefly materials and
phosphate buffer
- ATP + buffer + firefly materials == luminescence

38. ACKNOWLEDGMENT
Thank all people, teachers, friends, and your school that
supported you during your journey in AL-bairaq project.
Also don’t forget to thank Eng. Doaa from AL-Bairaq team and
the sponsors who gave you the opportunity to participate in AL-
Bairaq project, create and innovate.