1. Barcoding the Kelp Forests of
California’s Channel Islands National Park
Ralph Imondi, David Kushner, Daniel Distel, Timery Deboer, Robert Hanner,
and Linda Santschi

2. Caenorhabditis elegans Hirudo medicinalis Schistocerca nitens
Drosophila melanogaster Danio rerio Xenopus laevis
Gallus domesticus Rattus norvegicus Mus musculus

3. Energizing science through
interdisciplinary studies
concepts
environmental
earth science
science & policy
ecology biochemistry
molecular
cell biology developmental
biology
biology
tools processes
computer
genetics
science

4. Mending the disconnect between
scientific research and science education
scientific research community 2˚ educational community

5. Emphasizing the scientific discovery process
exploration
& discovery
TESTING
IDEAS
community
benefits
analysis
&
&
outcomes
feedback

6. Potential student entry points into the
DNA barcoding workflow
utilizing barcode data
application
specimen collecting and user
tissue sampling
research
user
BOLD
GenBank
EOL
general
public

7. Student engagement in the entire workflow
specimen collecting

8. tissue processing

9. creating specimen pages on BOLD

10. generating COI amplicons

11. analyzing, editing, and uploading sequence data

12. California’s northern Channel Islands

13. The northern Channel Islands are fringed by
extensive kelp forest habitat

14. Upwelling features near the islands
contribute to kelp forest productivity
*
* *
*

15. San Luis Obispo
California
Current
Oregonian
Province
Point
Conception
Santa Barbara
cold subartic
water Ventura
Los Angeles
warm equatorial
Pacific water
California
Countercurrent
Californian
Province

16. The confluence of major current systems
gives rise to ecologically diverse species
assemblages
San Miguel Santa Rosa Santa Cruz Anacapa
Pycnopodia helianthoides variableHypsypops zone
transition rubicundus
boreal special temperate
biota assemblage biota

17. The CINP monitors up to 37 sites around the
northern Channel Islands
San Miguel
Santa Cruz
Santa Rosa
Anacapa
the sites reflect the broad range of conditions and
biological assemblages found in the park

18. The park collects abundance data for select
species of algae, invertebrates, and fishes
C. pictus
O. californica C. gigantea
spadicea P. helianthoides
miniata C. punctipinnis
nicholsii
P. elegans
B. stearnsii K. rufescens
H. kelletii P. californica
M.californicus E. jacksoni
S. californicus
L. lofotensis
U. chilensis M. crenulata
H. rubicundus D. undosa
M. imbricata L. californica
O. dalli

19. Sebastes barcoding project
S. mystinus S. chrysomelas S. auriculatus S. rosaceus
S. dalli S. carnatus S. semicinctus S. serriceps
S. umbrosus S. atrovirens S. miniatus S. serranoides

20. number
of species
1-5
6 - 10
11 - 15
16 - 20
number21 species
of - 25
26 - 30
31 - 35
36 - 40
41 - 45
46 - 50
51 - 55
56 - 60

21. Rockfishes are a heavily exploited by
commercial and recreational fisheries
Pacific Ocean Perch
Widow
1.0
Yelloweye
Bocaccio
relative depletion
0.8 Darkblotched
Cowcod
0.6 Canary
0.4
0.2
0
1960 1965 1970 1975 1980 1985 1990 1995 2000 2005 2010

22. The inability to identify larval rockfish
hinders efforts to characterize their
distribution, abundance and life history traits

23. Forms of student engagement
Direct (residential program for students)
CMB
Indirect (professional development program for teachers)
training
instructional materials high
standardized lab protocols school
transportable equipment
CMB

24. Collaboration framework (scientific strand)
students
CINP resource management & enforcement personnel
UCSB Marine Operations Facility
volunteers
specimen
collection & tissue
processing
sample curation
& data accession
Ocean distribution
Genome CCDB
taxonomic Legacy
verification
& vouchering
SBMNH

25. Economic workforce development linkages
Recruitment Equipment support
CMB
high
schools
Residential program for students
Professional development program

26. Economic workforce development linkages
Recruitment
North Valley Biotech Center
Bay Area Biotech Center
Equipment support
LA/OC Biotech Center
SoCal Biotech Center

27. Geographic Distribution of Participants
Arcadia
Bakersfield
Calabasas
Camarillo
Carpinteria
Chatsworth
Compton
Dana Point
Dos Pueblos
Dublin
Fontana
Granada Hills
Hollywood
Huntington Beach
La Jolla
Lakewood
Long Beach
Los Angeles
Los Altos
Los Gatos 300 km
Los Osos
Mission Viejo
Moorpark
North Hollywood
Norwalk

28. Project status
NORTHERN CHANNEL ISLANDS SEBASTES BARCODE PROJECT [CMBS]

29. Project status
Channel Islands Kelp Forest and MPA Fish and Invertebrates [CNOI]

30. Geographic Distribution of Participants
300 km

31. Expanding student access to specimens
Klamath
Sierra
San
Nevada
Francisco
Bay Area
Mohave
Desert
Mediterranean Sonoran
Coast Desert

32. Alignment of student-led barcoding activities
with NPS I&M activities and youth programs

33. Technology Resource Development

34. Technology Resource Development
www.DNAbarcodingAssistant.org

35. Technology Resource Development
CMB Guelph meeting the
barcode data
standard
BOLD
user user
BOLD data portal

36. Technology Resource Development
Guelph meeting the
high barcode data
schools standard
BOLD
pre-BOLD
professional
non-professional user
user BOLD
professional data portal
user

37. Biotechnology ExplorerTM

38. An Internet platform to broaden student
engagement in DNA barcoding and iBOL

39. Acknowledgements
Robert Hanner
David Kushner Sujeevan Ratnasingham
Sonia Ibarra
Eric Mooney
Joshua Sprague Eric Hochberg
Paul Valentich-Scott
Dan Distel
Abigail Fusaro
Timery Deboer
Jeffery O’Neal
Milton Love Wendi Johnston
Samuel Hammond
Christoph Pierre