1. Bioconversion of Lignocellulosic waste (wheat straw) for the production of biofuel Desh Bandhu Gangwar

3. Cellulosic biomass is an alternative energy feed stock because supplies are abundant domestically and globally.

5. OBJECTIVE To use suitable physical and chemical method for treatment of agro-waste. Bioconversion of agro-waste into fermentable sugars. The production of ethanol.

6. METHODOLOGY Lignocellulosic material Pre - treatment Hydrolysis Fermentation Ethanol

7. SEPRATION OF HEMICELLULOSE, LIGNIN AND CELLULOSE COMPONENT OF WHEAT STRAW Wheat straw Steam Treatment Water soluble Insoluble (Hemicellulose) (Lignin + Cellulose) aq. Alkali Treatment soluble insoluble (Lignin) Adjust pH 7.0 Lignin Cellulose

8. RESULTS 1. Percent composition of hemicellulose, lignin and cellulose in wheat straw

9. 2.Effect of high pressure steaming 3.Estimation of total sugar concentration by by (Scott and Melvin, 1953) method. Extract 1 : Raw material treated with 1% acid (Concentrated H2SO4) Extract 2: Raw material treated with 2% NaOH (alkali)

10. 4. Estimation of total reducing sugar concentration by Miller et al., 1972) method. . 5.Estimation of total celluloseconcentration by (Updegraph , 1969) method

11. 6.Estimation of total cellulose concentration by (Updegraph, 1969) method 7.Estimation of total hemicelluloses concentration by (Bailey,1957) method.

12. Effect of temperature on ethanol production

13. Concentration of Ethanol Effect of pH on ethanol production(acid treated wheat straw Effect of pH on ethanol production(alkali treated wheat straw) 0.25 0.3 0.2 0.25 Concentration of Ethanol 0.15 0.2 pH 5 pH 5 0.15 pH 7 0.1 pH 7 0.1 0.05 0.05 0 0 1 2 3 4 1 2 3 4 Days Days Effect of pH on ethanol production