3. Corneal Endothelial Anatomy
Purpose of doing Specular Microscopy
Instrumentation (Eye Bank specular)
Basic Procedures
Normal and Abnormal
Conclusions
4.
5. Cell count of a Tissue
Quality of Tissue
Good cell count is essential before Corneal
Transplant
Shape variability (pleomorphism)
Size variability (polymegathism)
22. Obtain image prior to storage at 2-8 °C if
possible
After storage, warm for at least one hour
at room temperature
If cells are misshapen or image quality is
poor, warm longer and repeat
Strive for “good” or better quality images
25. When: as soon as possible
Delay results in poorer images
Immediately after laboratory excision if whole
globe procured
12 hours post-mortem 4 days post-mortem
29. Accurate endothelial cell density (ECD)
Pre-cut tissue: pre- and post-cut ECD
Presence of cell size or shape variability
Evidence of endothelial disease
36. Eyebank-specific findings:
•Forceps trauma may mimic guttae
•Corneal “stress lines” induced by corneal
folding during excision may mimic guttae
•Folds from edema/cooling
39. Every Technician / Staffs of Eye Bank should
be well trained to handle Specular
Microscope.
Always bring the tissue in room temperature
for better result.
Tissues must be handle with care.
Every Year calibration of Specular Microscope
is required.