GRM 2013: Implementing MARS Project for drought tolerance and the Cassava Breeding Community of Practice: Accomplishments in the GCP and the Years Ahead -- E Okogbenin
World: Cassava - Market Report. Analysis And Forecast To 2020
Similar a GRM 2013: Implementing MARS Project for drought tolerance and the Cassava Breeding Community of Practice: Accomplishments in the GCP and the Years Ahead -- E Okogbenin
Similar a GRM 2013: Implementing MARS Project for drought tolerance and the Cassava Breeding Community of Practice: Accomplishments in the GCP and the Years Ahead -- E Okogbenin (20)
GRM 2013: Implementing MARS Project for drought tolerance and the Cassava Breeding Community of Practice: Accomplishments in the GCP and the Years Ahead -- E Okogbenin
3. • Introduced by Federer (1956)
• Controls (check varieties) are replicated in a standard
experimental design
• New treatments (genotypes) are not replicated, or have
fewer replicates than the checks – they augment the
standard design
Augmented Designs
• Used in plant breeding during early generations
+ Seed, Land and other resources are limited
+ Want to evaluate as many genotypes as possible
+ Difficult to maintain homogeneous blocks when
comparing so many genotypes
•Unreplicated designs can make good use of scarce
resources
+ Evaluate more genotypes
+ Test in more environments
• Provide an estimate of standard error that can be used
for comparisons
+ Among the new genotypes
+ Between new genotypes and check varieties
9. Traits Minimum Maximum Average Std Dev Dakata
Number of Scars (6MAP) 25 63 44.85 8.93 35
Plant height (m: 6MAP) 62 187 98.11 24.15 90
Branching height (m) 51 190 82.87 24.24 81
Length of stems with scars
(m) 36 119 69.29 15.68 62
Number of leaves (6MAP) 20 308 89.43 60.17 90
Branching levels (6MAP) 1 3 1.12 0.43 1
Summary of morphology data or the selected Latin American and
elite local varieties evaluated at Minjibir,
10. Phenotyping
Phenotyping for key traits:
Photosynthesis
Stomatal conductance
Abscisic acid
Stem starch
Partner: Cornell university
16. The Task – second phase
(2011 -2013)
To increase routine use of markers in
breeding
Development of new generation of breeders
Improvement in data management and
analysis
To develop partial inbred lines
Gene pool development for farmer and end-
user key traits (architecture, yield, dry
matter)
18. Expansion of CoP
Old countries
Nigeria
Ghana
Tanzania
Uganda
New countries
Liberia
Kenya
Mozambique
DRC
Cote d’Ivoire
Malawi
South Sudan
Ethiopia
CG centers: IITA & CIAT
19. Routine use of markers in
Breeding
Marker-assisted selection
(MAS)
Mapping SNP markers
around CMD2 gene
Serial # Markers
1 NS 169
2 NS 890
3 NS 149
4 SSRY 83
5 SSRY 106
6 SSRY 103
7 SSRY 28
8 NS 158
9 NS 198
10 EME 425
11 EME 171_1
12 NS 162
13 NS 124
23. CoP workshops
First phase
IITA, Nigeria – 2008
NaCRRI, Uganda – 2009
CRI, Ghana - 2010
Second phase
ARI , Tanzania – 2012
SARI, Ethiopia - 2013
24. Development of genetic stocks
S2, S3 lines
Starch,
beta carotene
CBSD
Genetic stocks for CBSD tolerance in Tanzania
Hybridization
25. Release of MAS developed
varieties
CR 37-108 CR 36-2
CR 14A-1 CR 36-5
26. CoP
Breeding support through IBP
Training on Data management
ICASS
producing field books,
Genetic profiles for planning crosses,
Tools for adding new breeding methods, new data fields, and new traits