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Similar to Three types of Lentiviral Vectors - by GEG-tech.com
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Three types of Lentiviral Vectors - by GEG-tech.com
- 3. LentiVectors
The RNA vector genome is then transcribed
by the viral reverse-transcriptase (RT) into
linear DNA.
© 2014 GEG-Tech, all rights reserved
- 5. LentiVectors
Using the cell machinery, your protein of
interest is synthetized from the integrated
vector genome
© 2014 GEG-Tech, all rights reserved
- 7. Now, let’s see how a non-integrating
lentiviral vector works
© 2014 GEG-Tech, all rights reserved
- 10. If we disable the viral integrase…
© 2014 GEG-Tech, all rights reserved
- 13. EpiVectors
Due to the dilution effect of cell growth,
these circles can provide short-term
expression in dividing cells.
© 2014 GEG-Tech, all rights reserved
- 15. If you want transient expression of your
transgene even in non-dividing cells,
TransVectors are what you need.
How does it work ?
© 2014 GEG-Tech, all rights reserved
- 16. TransVectors
In a classic lentiviral vector, the reversetranscriptase binds to a specific region of the
vector genome called PBS
© 2014 GEG-Tech, all rights reserved
- 21. TransVectors
Since RNAs have a short lifetime in the
cytoplasm, the protein expression will be
highly transient, even in non-dividing cells.
© 2014 GEG-Tech, all rights reserved