1. ‫بسم ا الرحمن الرحيم‬
FAMILY:
VIBRIONACEAE
Prof. Khalifa Sifaw Ghenghesh

2. 3 Important Genera:
• Vibrio
• Aeromonas,
• Plesiomonas
• Oxidase +ve
Habitat:

3. ‫بسم ا الرحمن الرحيم‬
Vibrio cholerae
I. V. cholera O1:
• Agglutinate in Antisera Directed
Against O1 Antigen.
• Produce Enterotoxin.
2. Non-Toxigenic V. cholera O1:
3. Non O1 V. cholera (NCV, NAV):
II. V. cholerae O139

4. Vibrio cholerae
Gram-negative, curved rods

5. Vibrio cholerae
Leifson flagella stain

6. Vibrio cholerae
curved rods with polar flagellum (EM)

7. V. cholerae O1
--------------------------------------------------Biotye
Haemolysis (SRBC)
VP
El-tor
+
Classic
--
+
--
Resistance to PB
+
----------------------------------------------------

8. VIRULENCE FACTORS:
• Enterotoxin: CT
• Adherence: Ability to Attach to the
Microvilli at the Brush Borders of the
Epithelial Cells.
EPIDEMIOLOGY:
• Causes Widespread Disease.
• Human Carriers:
• Modern International Travel:

9. PATHOGENESIS & CLINICAL
INFECTION:
•
•
•
•
•
Infectious Dose:
Incubation period:
Abrupt Onset of Diarrhoea & Vomiting
Stool > Watery (Rice Water), Odorless
Fatality Rate:

10. LAB. DIAGNOSIS:
• Isolation & Identification of the
Organism.
• Media: TCBS
• Transport Media:
TREATMENT:
• Prompt Replacement of Fluid &
Elecrolytes
• Tetracycline and others
PREVENTION & CONTROL:
• Adequate Sewage Treatment and
Water Purification Systems.
• Vaccination: ??

11. Typical Vibrio cholera
contaminated water supply

13. Vibrio parahaemolyticus
• Halophilic vibrio
• Associated with seafood
• Gastroenteritis:
– Outbreaks of Food Poisoning
• Extraintestinal infections > wounds
• Virulence Factors:
– Haemolysin >
– heat-stable cytotoxin
– Heat-labile enterotoxin
– Adherence to human intestinal cells

14. Aeromonas species
A. hydrophila, A. sobria & A. caviae
• Enterotoxins, Cytotoxins, Haemolysins,
Etc…
• Gastroenteritis, Septicaemia, and Skin
& Wound Infections
TREATMENT
• TMP-SMX, Tetracycline, Gentamicin
• Resistant to β-Lactam Antibiotics.