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B. Assefa, D. Abate, B. Leta


First Bio-Innovate Regional Scientific Conference,
   Addis Ababa, Ethiopia, 25-27 February 2013




                             BioInnovate Project 04/2010   1
   Coffee is the world's second most valuable
    commodity after petroleum
   Ethiopia
    ◦ Contributes about 3 % for global coffee market.
    ◦ Produces about 380,000 tons
    ◦ About 16% coffee bean is produced by wet process
      while 84 % by dry process.




                                                              2
                                BioInnovate Project 04/2010
   The dry processing generates
    ◦ the solid waste (coffee husk)
    ◦ piled up in field causing serious
      environmental problem

   The wet processing generates
    ◦ The wastewater released untreated
    ◦ Solid waste with high moisture content
      (pulp and mucilage)
    ◦ Dispose in the water and field


                                                          3
                            BioInnovate Project 04/2010
   Estimated annual generation of solid
    wastes
    ◦ Husk 430,920 tons/year;
    ◦ Pulp 72,960 tons/year
   It is huge resources;




                                                      4
                        BioInnovate Project 04/2010
   Characteristics of coffee husk

     No        Parameters                    Mean
      1   Moisture content (%)               9.63
                                 3
     2    Bulk Density (mg/cm )               0.74
     3    Volatile matter (%)                 82.7
     4    Fixed matter (%)                    7.65
     5    Ash (%)                            7.65
     6    Total Carbon (%)                   53.67


                                                           5
                             BioInnovate Project 04/2010
   Characteristics of the Pulp

            Parameters             Mean
       TS, %                       70.13
       VS/TS, %                     93.0
       Moisture Content, %         29.87
       Fixed Solid, %               6.99
       Total Carbon (%)            50.18
       pH                             6.8


                                                           6
                             BioInnovate Project 04/2010
   The waste has high organic content
   It can be a good feed stock for biogas
    production
   Concern
    ◦ The biomass might be cellulosic materials
    ◦ the rate of biodegradability may be low
    ◦ Tannin, caffeine and phenols could poison
      anaerobic bacteria




                                                           7
                             BioInnovate Project 04/2010
◦ Decomposing the biomass using (oyster) mushroom
  would improve biodegradility
◦ Reduce the poisoning effect of TCP chemicals using
  the use of biomass for mushroom




                         BioInnovate Project 04/2010   8
   Determination of biogas potential of coffee
    husk, coffee husk spent after mushroom
    production and pulp
   Comparison of the results against the
    hypothesis




                                                         9
                           BioInnovate Project 04/2010
Materials
   The inoculums
    ◦ brought from an active, food leftover based biogas
      plant found in the same institution;
    ◦ Amount of inoculums: 800 ml (volume);
   Substrates:
    ◦ Coffee pulp, coffee husk, and spent coffee husk.
    ◦ The pulp are kept in a deep freezer (less than -
      120C)
    ◦ The spent is brought from a mushroom cultivation
      experiment (after three cycle of harvest)

                                                            10
                              BioInnovate Project 04/2010
11
BioInnovate Project 04/2010
   Composition of substrate added into the flasks

        Substrates    TS (%) VS (%)
        Husk          87.5           80.8
        Spent         27.8           89.8
        Pulp          12.1           79.9
        Inoculums     2.3            63.3

                                                           12
                             BioInnovate Project 04/2010
Apparatus
◦   Erlenmeyer Flask
                                     —        Gas analyzer
◦   Tubes
                                     —        Syringe
◦   Water bath (Thermostat)
                                     —        Balance
◦   Gas bags
                                     —        pH meter
◦   Shredder
                                     —        Stop cock
◦   Muffle furnace
◦   Drying oven




                                                             13
                              BioInnovate Project 04/2010
Methods
◦ The samples are added in 1:2 ratio VS of
  substrate to inoculum in order maintain
  constant food to micro-organism ratio
◦ the experiments were run at 37oC
◦ The temperature is maintained by
  thermostat
◦ The pH is measured initially and at the
  end of the experimentation
◦ Gas is collected regularly by gas bag
                                                    14
                      BioInnovate Project 04/2010
Measurement
   Mass of substrate
    ◦ Precision balance is used
   Gas volume
    ◦ the volume of the gas was measured using syringe
      of known volume, 50ml and100 ml.
   Temperature
    ◦ the temperature of the room is measured regularly
      when the gas volume is measured using
      (thermometer)thermocouple


                                                          15
Experimental Set up




               BioInnovate Project 04/2010   16
BioInnovate Project 04/2010   17
Biogas yield [l/g Methane gas
Items         VS]         [l/g VS] [%]
 Pulp    0.863 0.081 0.460          56
 Husk    0.486 0.071       0.266    58
Spent    0.188 0.068       0.090    49


                     BioInnovate Project 04/2010   18
   Pulp gave output as of comparable to
    ◦ Organic wastes from food industry : 0.4-
      0.8 m3 biogas/kgVS (Gunaseela, 1997)
    ◦ Fruit and vegetables solid waste and
      wastewater 0.2-0.4 m3 CH4/kgVS
   Husk gave output as of comparable to
    ◦ Crops (straw, beet collars) 0.35-0.4 m3biogas/kgVS
    ◦ Rice straw 0.2 m3 CH4/kgVS (Dinuccio et al., 2009)
    ◦ Barley straw 0.2 m3 CH4/kgVS (Dinuccio et al., 2009)




                               BioInnovate Project 04/2010   19
   Spent substrate gave the lowest
    output
    ◦ It is lower estimated potential all biomass
    ◦ It seems that the mushroom taken up
      significant easily digestible stuff




                           BioInnovate Project 04/2010   20
◦ Coffee waste can be as a good feed for
  biogas production
◦ Coffee husk much higher gas potential
  than spent coffee husk
  The use of coffee husk for mushroom
   production did not improve gas
   production and its rate
◦ The pulp collected from wet processing
  gives much higher gas production
  coffee husk
◦ Spent coffee husk has still significant
  gas potential

                     BioInnovate Project 04/2010   21
Thank for your
attention


         BioInnovate Project 04/2010   22

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Biogas potential of spent, coffee husk and pulp

  • 1. B. Assefa, D. Abate, B. Leta First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013 BioInnovate Project 04/2010 1
  • 2. Coffee is the world's second most valuable commodity after petroleum  Ethiopia ◦ Contributes about 3 % for global coffee market. ◦ Produces about 380,000 tons ◦ About 16% coffee bean is produced by wet process while 84 % by dry process. 2 BioInnovate Project 04/2010
  • 3. The dry processing generates ◦ the solid waste (coffee husk) ◦ piled up in field causing serious environmental problem  The wet processing generates ◦ The wastewater released untreated ◦ Solid waste with high moisture content (pulp and mucilage) ◦ Dispose in the water and field 3 BioInnovate Project 04/2010
  • 4. Estimated annual generation of solid wastes ◦ Husk 430,920 tons/year; ◦ Pulp 72,960 tons/year  It is huge resources; 4 BioInnovate Project 04/2010
  • 5. Characteristics of coffee husk No Parameters Mean 1 Moisture content (%) 9.63 3 2 Bulk Density (mg/cm ) 0.74 3 Volatile matter (%) 82.7 4 Fixed matter (%) 7.65 5 Ash (%) 7.65 6 Total Carbon (%) 53.67 5 BioInnovate Project 04/2010
  • 6. Characteristics of the Pulp Parameters Mean TS, % 70.13 VS/TS, % 93.0 Moisture Content, % 29.87 Fixed Solid, % 6.99 Total Carbon (%) 50.18 pH 6.8 6 BioInnovate Project 04/2010
  • 7. The waste has high organic content  It can be a good feed stock for biogas production  Concern ◦ The biomass might be cellulosic materials ◦ the rate of biodegradability may be low ◦ Tannin, caffeine and phenols could poison anaerobic bacteria 7 BioInnovate Project 04/2010
  • 8. ◦ Decomposing the biomass using (oyster) mushroom would improve biodegradility ◦ Reduce the poisoning effect of TCP chemicals using the use of biomass for mushroom BioInnovate Project 04/2010 8
  • 9. Determination of biogas potential of coffee husk, coffee husk spent after mushroom production and pulp  Comparison of the results against the hypothesis 9 BioInnovate Project 04/2010
  • 10. Materials  The inoculums ◦ brought from an active, food leftover based biogas plant found in the same institution; ◦ Amount of inoculums: 800 ml (volume);  Substrates: ◦ Coffee pulp, coffee husk, and spent coffee husk. ◦ The pulp are kept in a deep freezer (less than - 120C) ◦ The spent is brought from a mushroom cultivation experiment (after three cycle of harvest) 10 BioInnovate Project 04/2010
  • 12. Composition of substrate added into the flasks Substrates TS (%) VS (%) Husk 87.5 80.8 Spent 27.8 89.8 Pulp 12.1 79.9 Inoculums 2.3 63.3 12 BioInnovate Project 04/2010
  • 13. Apparatus ◦ Erlenmeyer Flask — Gas analyzer ◦ Tubes — Syringe ◦ Water bath (Thermostat) — Balance ◦ Gas bags — pH meter ◦ Shredder — Stop cock ◦ Muffle furnace ◦ Drying oven 13 BioInnovate Project 04/2010
  • 14. Methods ◦ The samples are added in 1:2 ratio VS of substrate to inoculum in order maintain constant food to micro-organism ratio ◦ the experiments were run at 37oC ◦ The temperature is maintained by thermostat ◦ The pH is measured initially and at the end of the experimentation ◦ Gas is collected regularly by gas bag 14 BioInnovate Project 04/2010
  • 15. Measurement  Mass of substrate ◦ Precision balance is used  Gas volume ◦ the volume of the gas was measured using syringe of known volume, 50ml and100 ml.  Temperature ◦ the temperature of the room is measured regularly when the gas volume is measured using (thermometer)thermocouple 15
  • 16. Experimental Set up BioInnovate Project 04/2010 16
  • 18. Biogas yield [l/g Methane gas Items VS] [l/g VS] [%] Pulp 0.863 0.081 0.460 56 Husk 0.486 0.071 0.266 58 Spent 0.188 0.068 0.090 49 BioInnovate Project 04/2010 18
  • 19. Pulp gave output as of comparable to ◦ Organic wastes from food industry : 0.4- 0.8 m3 biogas/kgVS (Gunaseela, 1997) ◦ Fruit and vegetables solid waste and wastewater 0.2-0.4 m3 CH4/kgVS  Husk gave output as of comparable to ◦ Crops (straw, beet collars) 0.35-0.4 m3biogas/kgVS ◦ Rice straw 0.2 m3 CH4/kgVS (Dinuccio et al., 2009) ◦ Barley straw 0.2 m3 CH4/kgVS (Dinuccio et al., 2009) BioInnovate Project 04/2010 19
  • 20. Spent substrate gave the lowest output ◦ It is lower estimated potential all biomass ◦ It seems that the mushroom taken up significant easily digestible stuff BioInnovate Project 04/2010 20
  • 21. ◦ Coffee waste can be as a good feed for biogas production ◦ Coffee husk much higher gas potential than spent coffee husk  The use of coffee husk for mushroom production did not improve gas production and its rate ◦ The pulp collected from wet processing gives much higher gas production coffee husk ◦ Spent coffee husk has still significant gas potential BioInnovate Project 04/2010 21
  • 22. Thank for your attention BioInnovate Project 04/2010 22