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1.
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3. -It's a type of electromagnetic
spectroscopy which analyze
fluorescence from a sample.
- it involves using abeam of light
, usually ultraviolet light that
excites the electron in molecule of
certain compound and causes them
to emit light of a lower energy
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15. CONCENTRATION AND FLUORESCENCE INTENSITY :
The power of fluorescent radiation, F, is proportional to the
radiant power of the excitation beam absorbed by the species
able to undergo fluorescence:
F = K'(P0 - P)
where P0 is the power incident on the sample, P is
the power after it traverses a length b of the solution and K' is
a constant which depends upon experimental factors and the
quantum efficiency of fluorescence.
Beer's law can be rearranged to give:
P/P0 = 10- bc
where A = is the absorbance.
bc
Substitution gives:
F = K'P0(1 - 10- bc)
This is the fluorescence law
Unlike Beer’s Law fluorescence isn’t in general linear with
concentration
This expression can be expanded (Taylor series):
To a good approximation if is small (< 0.05) the higher-
bc
order terms are nearly zero, we have:
F = 2.3K'bcP0
16.
17. -which demonstrates two
important points:
1-that at low concentrations
fluorescence intensity is
proportional to concentration;
-at the absorption frequency
For a concentration above c1
the calibration curve is no
longer linear
2-that fluorescence is
proportional to the incident
power in the incident radiation
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20. http://en.wikipedia.org/wiki/Fluorescence_spectroscop
y
http://medical-
dictionary.thefreedictionary.com/fluorometry
http://www.answers.com/topic/fluorometry
http://www.google.com.eg/search?q=Fluorometry&hl=ar
&gbv=2&prmd=imvnsb&tbm=isch&tbo=u&source=univ&sa
=X&ei=iwbXTsiBCcmv8gOtoOjMDQ&ved=0CJMBELAE&biw=
1280&bih=656
21. 1- Aya Ahmed Saber Yosif 86
2- Jehan Essam Mahmoud 112
3- Eman Mohammed Mostafa 83
4- Basma Sayed Saeed Ahmed
5- Bosy Nabil
6- Ayat Fwzy
7- Eman Gamal
8- Gourge Reda
9- Andro Esmat
10- Jhon Esmat
