1. E coli, Klebsiella and Enterobacter Prof M.I.N. Matee School of Medicine MUCHS

24. Klebsiella spp

27. K pneumoniae and K oxytoca Hospital acquired infections due to K rhinoscleromatis produces rhinoscleroma, condition with destructive granuloma of the nose and pharynx . K ozenae – progressive atrophy of nasal mucous membrane

31. Diagnosis of Bacterial Infection culture on plates or in broth identification by biochemical or serological tests on pure growth from single colony microscopy Decolorise Counterstain Stain unstained or stained with e.g. Gram stain sensitivities Serodiagnosis DNA technologies by disc diffusion methods, breakpoints or MICs

32. Gram negative rods

33. Lactose fermentor on MCA

34. Lactose positive Klebsiella sp

35. E. coli

39. A=Acidic K=Alkaline - - + - - GAS + + - - - HYDROGEN SULFIDE A A A A - BUTT K K A A - SLANT TUBE 5 TUBE 4 TUBE 3 TUBE 2 TUBE 1 (UNINOCULATED) INTERPRETATION OF TUBES ABOVE

52. Thank you