1. Done by:
Roaa Al-shareef .
Supervisor :
Mrs. Hibah Abu-Sulaiman

2.  Lasttime we were chefs
agar, broth .
 ThisPicasso we are !
culturing, staining.

3.  Culturing techniques.
 Condition of incubation.
 Reading culture plates.
 Preparing bacterial smears.
 Staining using Gram Stain.

4.  To draw our pictures colors :
Sample

5.  for Picasso's environment :
Cleaning the paintbrush .
Bring the painting board .
Drowning then painting.
Cleaning the paintbrush.

6.  For laboratory medicine environment :

7.  In
order to get an excellent staining and
chemical tests .. colonies should be gotten.
A colony is gotten in a appropriate Petri
dish .

9.  Microorganisms are similar to more complex
organisms in that they need a variety of
materials from their environment to function
and to live, which is :
Suitable Temperature .
Suitable Ph .
Air content .
SALINITY.
Pressure .

10.  Organisms are sensitive to changes in
temperature because the folding of the
proteins and DNA in the cell can be
disturbed.
What do you think will happen if you
heated protein !!
AND
What do you think will happen if you freeze
it !!

11.  Both the use of heat and cold allows for
control of bacterial growth by either
destroying the microbe (heat) or
preventing further growth (cold).

12.  According to temperature :

13.  require temperatures
above 80°C .

14. Again..!
Organisms are sensitive to changes in pH
because the folding of the proteins and
DNA in the cell can be disturbed.
What do you think is the suitable PH for
bacterial growth !

17. Other types of bacteria :
Microaerephilic:
This organisms live
with low concentration
of oxygen.
Capnophilic:
these organisms must
have an environment rich
in carbon dioxide.

18.  there is certain categorize to read colonies
according to
shape
Size
Texture
color
other features.

20.  The ability to alter or destroy the red blood cells and
create a color change in the medium.
There is :
A .Beta hemlysis :
colonies are surrounded by a clear zone.
B.Alpha hemolysis:
colonies are surrounded by a zone greenish
Color.
C.Gama hemolysis:
No hemolysis.

21.  size :
The colonies either big or small,
maybe in between .
 margin :
The colonies present a smooth
Margin , filamentous, irregular or
Rough.

22.  Elevation :
The colonies rise from
The plate forming an elevations
 Color :
Colonies present a basic colors:
White , yellow , gray .