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Gluten Test Kit Methods Usda
1.
Commercial Gluten Test
Kits / Methods Jupiter Yeung, PhD Principal Scientist Food Products Association FDA Gluten Free Meeting, Aug 19, 2005
2.
3.
22 Capture Antibody
Detector Antibody Signal Sandwich ELISA
4.
5.
Commercial Gliadin ELISA
Kits * ppm gliadin ppm gluten = ppm gliadin x 2 Converted to ppm from g/mL Company Product LOD* LOQ* Range* Replicates Costs Neogen Alert (Qualitative) 10 1 $237/20Sampl Veratox 5 5-50 1 $465/38Sampl R-Biopharm RidaScreen 1.5 2.5 2.5-40 2 $425/96 wells RidaScreen Fast 5 5 5-40 1 $400/48 wells Tepnel Gluten Assay Kit, AOAC or High sensitivity 5 50 50-1000 2 $425/96 wells 0.5 5 5-100 2 Hallmark HAVen Gluten, AOAC or High sensitivity 5 50 50-1000 2 $425/96 wells 0.5 5 5-100 2 Diffchamb Transia Plate Gluten 5 5 5-100 2 $500/96 wells Transia Plate Prolamins 1.5 0.8 0.8-12.5 2 $500/96 wells Morinaga Wheat Protein 0.3* 3.12 3.12-20 2 $600/96 wells
6.
Commercial Gliadin ELISA
Kits Company Product Validation Neogen Alert Veratox R-Biopharm RidaScreen RidaScreen Fast PWG ring trial Tepnel BioKit Gluten (AOAC OM) High Sensitivity Hallmark HAVen Gluten (AOAC OM) High Sensitivity Diffchamb Transia Plate Gluten NFA, Sweden Transia Plate Prolamins Morinaga Wheat Protein NIHS, Japan
7.
0 5
10 20 50 ppm gliadin controls Gliadin ELISA
8.
9.
Lateral Flow Device
10.
Lateral Flow Device
11.
Lateral Flow Device
12.
13.
14.
15.
16.
Effect of Partial
Hydrolysis Ferre et al. Proceeding of the 18 th Meeting, WPGAT, 2004
17.
18.
19.
Garcia et al.,
Proceeding of the 18 th Meeting, WPGAT, 2004
20.
Detection & Speciation
Wheat Gliadins Rye Scalins Barley Hordeins Oat Avenins Ab-based + - PCR + + + + MS + + + +
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