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Final Glory PPT.pptx
1. GC-MS Analysis and Inhibitory evaluation of
extracts of Kavuni rice on major enzymes
linked to Diabetes: An In-vitro & In-silico
Approach
Under the Guidance of
Prof. S. Umamaheswari
Presented by
E. Glory Arul Blessy
(Reg No. 20204012502105)
2. Diabetes mellitus
Diabetes mellitus - group of metabolic
diseases characterized by chronic
hyperglycemia resulting from defects in insulin
secretion,/insulin action/both.
Chronic hyperglycemia of diabetes is
associated with long-term damage, dysfunction,
and failure of different organs (eyes, kidneys,
nerves, heart & blood vessels).
Diabetes mellitus is rising to an alarming
epidemic level.
Major cause of Diabetes in recent years
Higher consumption of polished white rice
Ref: Akram and Hisham, 2015
3. White rice and Diabetes
• Undergoes higher degree of
milling process
• Devoid of essential
micronutrients
• Starchy endosperm-higher
carbohydrate levels
• High Glycemic index & High
Glycemic Load: increased risk
of type 2 diabetes
• Lower levels of bioactive
substances
• Lower levels of dietary fiber
and polyphenols
Ref: Q Sun et al., 2010
4. Why pigmented rice varieties is preferred?
• Valuable Nutrient Content
• Low Glycemic Index
• Rich in Antioxidants like
Anthocyanins
• Presence of Polyphenols-
Antioxidants
• Undergoes less degree of
milling process
• Reduce the risk of metabolic
disorders
• Richer in Dietary fiber,
minerals and vitamins
• Reduction of LDL Cholesterol
• Higher concentration of
bioactive substances
Ref: TS Rathna Priya et al., 2019
5. Red Kavuni Rice
Pigmented and Traditional Rice Variety
Indigenous to Tamil Nadu
Grown mainly in Tanjore, Tirunelveli,
Dharmapuri & Kanyakumari Districts
Contains anthocyanin (antioxidant)
Contains higher levels of amylose, dietary
fiber, protein, β- carotene, lutein and
polyphenols than white rice.
Inhibitory potential against α-amylase and
β-glucosidase
Ref: Valarmathi et al., 2016
6. Objectives
Preparation of Rice Extract and
Phytochemical Screening
In vitro Antioxidant Assay and In vitro
Antidiabetic Assay
GCMS Analysis of the Rice Extract
Analysis of Drug-Likeness Property of
Phytocompounds using SWISSADME
Docking Studies of the Phytocompounds with
receptors related with Diabetes mellitus
7. Methodology
Rice Extract Preparation
Sample: Kavuni rice
Method: Soxhlet Extraction
Solvent: Methanol Ref: Choudhury et al., 2020
Phytochemical Screening
Tests the presence/absence of
Carbohydrates
Protein
Alkaloid
Phenol
Flavanoid
Tannin
Saponins
Anthocyanins Ref: Mannan et al., 2013
8. GC-MS Analysis
To profile the volatile bioactive compounds present in the methanol extract
of Kavuni rice.
(Ref: Ashok Kumar et al., 2020)
Analysis of Drug-Likeness Property of Bioactive compounds using
SWISSADME
To evaluate the pharmacokinetics, drug-likeness properties of the Bioactive
compounds present in the rice extract.
• Physicochemical properties
• Lipophilicity
• Water solubility
• Pharmacokinetics
• Drug-likeness
• Medicinal Chemistry
(Ref: Daina et al.,2017)
9. Molecular Docking Studies
To analyse the binding efficiency of the bioactive compounds of the rice
extract and to find out crucial residues involved in bond formation against
the enzymes (α-amylase & α-glucosidase)
Preparation of Receptor (α-amylase & α-glucosidase)
Preparation of Ligand
Docking studies using PatchDock
Interaction analysis using PyMol, LigPlot & Discovery Studio
(Ref: Ladokun et al., 2018)
24. Binding of Ferulic acid with Alpha Amylase Interaction of Trans-O-Hydroxy Cinnamic acid
with Alpha Amylase
Interaction of Ferulic acid with Alpha
Glucosidase
Interaction of Ferulic acid with Alpha
Glucosidase
Binding Analysis of Ferulic acid and Trans-O-Hydroxy Cinnamic acid with
Alpha Amylase & Alpha Glucosidase
25. Interaction Analysis Using Discovery Studio
Interaction of Ferulic acid with Alpha Amylase Interaction of Trans-O-Hydroxy Cinnamic acid
with Alpha Amylase
Interaction of Ferulic acid with Alpha Glucosidase Interaction of Trans-O-Hydroxy Cinnamic acid with
Alpha Glucosidase
26. Conclusion
• Kavuni rice has medicinal values since it contained a number of secondary
metabolites with potent antioxidant and Antidiabetic properties
• The presence of bioactive compounds with potent biological and
pharmacological activity showed the therapeutic potential of the rice.
• Further, In-silico studies confirmed the potent inhibition of the alpha
amylase and alpha glucosidase with good binding energies.
• Hence it can be concluded that the methanol extract of Kavuni rice has
good medicinal properties and it will be employed in the treatment and
management of Diabetes Mellitus.
27. References
• Sun, Q., et al., White rice, brown rice, and risk of type 2 diabetes in US men and women. Arch Intern
Med, 2010. 170(11): p. 961-9.
• Rathna Priya, T., Eliazer Nelson, A.R.L., Ravichandran, K. et al. Nutritional and functional
properties of coloured rice varieties of South India: a review. J. Ethn. Food 6, 11 (2019).
• Choudhury, Paramita., et al., (2020). Assessment of nutritional value and quantitative analysis of
bioactive phytochemicals through targeted LC‐MS/MS method in selected scented and pigmented
rice varietals. Journal of Food Science. 85. 10.1111/1750-3841.15164.
• Mannan, Mohammad et al.,(2013). Screening of phytochemical compounds and antioxidant
properties in local and HYV of Bangladeshi rice (Oryza sativa L.) International Journal of
Biosciences (IJB). 3. 151-160. 10.12692/ijb/3.4.151-160.
• Ashokkumar K et al.,(2020) Comparative Profiling of Volatile Compounds in Popular South Indian
Traditional and Modern Rice Varieties by Gas Chromatography–Mass Spectrometry Analysis. Front.
Nutr. 7:599119. doi: 10.3389/fnut.2020.599119
• Daina, A., Michielin, O. & Zoete, V. SwissADME: a free web tool to evaluate pharmacokinetics,
drug-likeness and medicinal chemistry friendliness of small molecules. Sci Rep 7, 42717 (2017).
• Ladokun et al.,(2018). GC-MS and molecular docking studies of Hunteria umbellata methanolic
extract as a potent anti-diabetic. Informatics in Medicine Unlocked. 13. 10.1016/j.imu.2018.08.001.
• Mohammad Ajmal Ali., 2020.,Molecular docking and molecular dynamics simulation of anticancer
active ligand ‘3,5,7,3′,5′-pentahydroxy-flavanonol-3-O-α-L-rhamnopyranoside’ from Bauhinia
strychnifolia Craib to the cyclin-dependent protein kinase, Journal of King Saud University -
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