Conferenza di Carlo Alberto Redi "Il futuro delle cellule staminali" in occasione della Mostra Darwin 1809-2009

1. Milano 16 luglio 2009
Palazzo Besana
ore 18.30
Il futuro
delle
cellule staminali
CarloAlberto Redi
Accademia dei Lincei
Università degli Studi di Pavia
Fondazione IRCCS
Policlinico San Matteo - Pavia
c.redi@smatteo.pv.it

3. Diagramma di Valentin Häcker (1892)
con cellule staminali nell’embrione di Cyclops

4. Diagramma di Artur Pappenheim (1905)
per illustrare la ematopoiesi

5. Stem cell basic biology

8. Crescita e differenziamento
neonato
sviluppo adulto
embrionale
zigote meiosi
meiosi
sperm
fecondazione
oocita

19. Lialm Donaldson report, June 2000

27. SCAFFOLDS, CELLULE STAMINALI E
BIOREATTORI PER LA PRODUZIONE DI
TESSUTO OSSEO BIOINGEGNERIZZATO
F. Benazzo and the components of CIT

28. Punched Titanium
SEM 50 X
Anti-osteocalcin SEM 450 X

40. surviving graft 10 ye ars after transplantation
of human fetal dopamine neurons to the right
striatum
Positron emission tomography scan
Olle Lindvall, “Stem cell transplantation”
Variazione della captazione di 18F-
fluorodopa nei cervelli di pazienti
con malattia di Parkinson dopo
trapianto, dimostrato dall‘esame
PET

42. 1 106

50. !! Cytoplast !!
!! Cytoplast !!

54. TELOMERE’ LENGTH AT DIFFERENT TIME
POINTS OF EXPANSION
WEEKS OF KMESF Kb
CULTURE
0 17.7 8.8
+2 21.1 10.4
+4 20.7 10.2
+5 18.6 9.8
+6 18.4 9.1
(FLOW-FISH)

56. retrotransfections (stemness & oncogenes)
Yamanaka and coworkers (2006/07): ≈1 on 5,000 ≈ 0.02%
Thomson and coworkers (2007): ≈ 1 on 10,000 ≈ 0.01%
cytoplasts (insulinoma & stem cell extracts)
Collas and coworkers (2004): ≈1 on 2 ≈ 50%
Redi and coworkers (2007): ≈ 1 on 3,000 ≈ 0.03%

57. ! ! !
biotechnology
stem cells
cloning

59. NO
stem cells

60. objections to reproductive
biotech
We should not play God

62. HeLa cells
HeLa cells should be given the new species
name of Helacyton gartleri.
The cells are a genetic chimera of HPV18 and
human cervical cells and now have a distinct,
stable, non-human chromosome number.
HeLa cells
since 1951 self-replicating
used in thousands biol exp
Henrietta Lacks contribution to medicine
1920 - 1951
HeLa cells
HeLa cancer cells have evolved into a
self-replicating, single-cell life-form.