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• Any biology based technology which uses organisms or
  their parts to make or modify products, or improve plants,
  animals and microorganisms.




BIOTECHNOLOGY
Genetic
engineering and
biotechnology
intertwining
concepts
Presented before class by:
AVILA, ERNIE C
BSE 4B
1.   Tissue and cell culture
2.   Marker or molecular fingerprint technology
3.   Discovery and cloning of genes
4.   Recombinant DNA technology or Genetic engineering.




Advances in S&T have
brought us:
• Serve as fingerprints and used in
a. mapping genomes,
b. Diagnostic tools to detect diseases or disorder in plants,
   animals including human
c. Facilitate in selection in plant and animal breeding and
   make it more effective and exact
d. Study biological diversity
e. Identify species variety or hybrid plants and animals,
   human individuals

1. Molecular and
biochemical markers
• Used in:
a. Medicine to propagate cell culture to produce health
   products such as hepatitis vaccine, insulin.
b. Agriculture-
 Propagate plants from a very small sections of various
   plant parts such as seed, roots, leaf and other. This will
   result to clone of similar and uniform quality




2. Tissue and cell culture
 Create variability or change in plants. Important traits
  such as resistance to disease can be obtained in this
  manner
 Eliminate diseases to produce disease-free planting
  materials which can be safely transported to other places.
 Help in designing drugs to combat diseases
 To incorporate such genes for important traits like disease
  resistance, control of ripening in crops by genetic
  engineering.




3. Discovery and cloning
of important genes
• An organism which has a gene transferred by genetic
  engineering is called as genetically modified organism
• Gen Eng. Is utilized only when the trait to be transferred
  is difficult or impossible and it will take long time to
  attain, by conventional breeding methods.




Genetically modified
products
• There are now many GM crops such as corn, cotton,
  soybean, oil rapeseed, papaya and tomato with traits
  obtained through genetic engineering.
• These traits includes resistance to insect pest such as corn
  and cotton borer, herbicide resistance, fugal resistance
  and viral diseases, altered soil composition and long shelf
  life.



Application in food and
agriculture
• Various crops with better nutritional, post harvest and
  processing qualities.
• Crops that thrive on:
o Saline soils
o Less water
o Less nutrients




Future products:
• Current:
 Production of biodegradable plastics by microorganism
 Use of recombinant chymosin in cheese production
 Use of genetically engineered glucose isomerase enzyme
  for high fructose syrup production which has a high
  thermal stability.




Industrial application
• Future benefits:
 Production of biodegradable plastics and other industrial
  polymers by plants
 Use of protein engineered industrial enzymes that reduce
  energy and raw material used.
 Production of processed foods with desired functional
  properties.
• Current benefits:
 Bioremediation using selected microorganism
 Use of transgenic crops which protects themselves from
  insect pest and pathogen, thus reducing tremendously the
  use of chemical pesticide.




Application in
environment
• Future benefits
 Development of crops which use less chemical fertilizer
  and less water
 Development of crops which produce less wastes and
  toxins and extract pollutants.
• Our growing population is expected to reach 104 million
  in 2020.
• To feed this population, our price production has to be
  increased from 12.3 million tons to 17.8 M or a 40%
  increase.
• These increase must come primarily from higher
  biological crop yields and not by increasing land area or
  using more nutrients and water.



What is biotechnology in
Philippines?
• Studies by UNDP, FAO, and World Bank shows that
  conventional method, even at their optimum efficiencies,
  will not be able to meet the food requirement of the
  increasing population. Such increases must be done in the
  context of sustainable agriculture.

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Genetic enginnering introduction

  • 1.
  • 2. • Any biology based technology which uses organisms or their parts to make or modify products, or improve plants, animals and microorganisms. BIOTECHNOLOGY
  • 4. 1. Tissue and cell culture 2. Marker or molecular fingerprint technology 3. Discovery and cloning of genes 4. Recombinant DNA technology or Genetic engineering. Advances in S&T have brought us:
  • 5. • Serve as fingerprints and used in a. mapping genomes, b. Diagnostic tools to detect diseases or disorder in plants, animals including human c. Facilitate in selection in plant and animal breeding and make it more effective and exact d. Study biological diversity e. Identify species variety or hybrid plants and animals, human individuals 1. Molecular and biochemical markers
  • 6. • Used in: a. Medicine to propagate cell culture to produce health products such as hepatitis vaccine, insulin. b. Agriculture-  Propagate plants from a very small sections of various plant parts such as seed, roots, leaf and other. This will result to clone of similar and uniform quality 2. Tissue and cell culture
  • 7.  Create variability or change in plants. Important traits such as resistance to disease can be obtained in this manner  Eliminate diseases to produce disease-free planting materials which can be safely transported to other places.
  • 8.  Help in designing drugs to combat diseases  To incorporate such genes for important traits like disease resistance, control of ripening in crops by genetic engineering. 3. Discovery and cloning of important genes
  • 9. • An organism which has a gene transferred by genetic engineering is called as genetically modified organism • Gen Eng. Is utilized only when the trait to be transferred is difficult or impossible and it will take long time to attain, by conventional breeding methods. Genetically modified products
  • 10. • There are now many GM crops such as corn, cotton, soybean, oil rapeseed, papaya and tomato with traits obtained through genetic engineering. • These traits includes resistance to insect pest such as corn and cotton borer, herbicide resistance, fugal resistance and viral diseases, altered soil composition and long shelf life. Application in food and agriculture
  • 11. • Various crops with better nutritional, post harvest and processing qualities. • Crops that thrive on: o Saline soils o Less water o Less nutrients Future products:
  • 12. • Current:  Production of biodegradable plastics by microorganism  Use of recombinant chymosin in cheese production  Use of genetically engineered glucose isomerase enzyme for high fructose syrup production which has a high thermal stability. Industrial application
  • 13. • Future benefits:  Production of biodegradable plastics and other industrial polymers by plants  Use of protein engineered industrial enzymes that reduce energy and raw material used.  Production of processed foods with desired functional properties.
  • 14. • Current benefits:  Bioremediation using selected microorganism  Use of transgenic crops which protects themselves from insect pest and pathogen, thus reducing tremendously the use of chemical pesticide. Application in environment
  • 15. • Future benefits  Development of crops which use less chemical fertilizer and less water  Development of crops which produce less wastes and toxins and extract pollutants.
  • 16. • Our growing population is expected to reach 104 million in 2020. • To feed this population, our price production has to be increased from 12.3 million tons to 17.8 M or a 40% increase. • These increase must come primarily from higher biological crop yields and not by increasing land area or using more nutrients and water. What is biotechnology in Philippines?
  • 17. • Studies by UNDP, FAO, and World Bank shows that conventional method, even at their optimum efficiencies, will not be able to meet the food requirement of the increasing population. Such increases must be done in the context of sustainable agriculture.