SlideShare una empresa de Scribd logo
1 de 12
Descargar para leer sin conexión
PARIOFORMA




                        Summary Report – September 2010

PARIOFORMA LTD
55 Princes Gate
Exhibition Road
London SW7 2PN
United Kingdom
Tel: +44 (0) 20 7225 3538
www.parioforma.com
PARIOFORMA




   Real-time PCR is becoming a mature technique.
   Instruments are very robust and reliable, and optimised assays can be ordered from multiple
    vendors.
   Although the technique will be further refined and more niche products will be developed, main
    future improvements are not expected within the qRT-PCR technology itself.
   Growth drivers include:
    ◦   Increasingly robust assays
    ◦   Automation
    ◦   Increasing demand for high-throughput
    ◦   Multiplexed analytical strategies
    ◦   Increasingly affordable machines
   Technology trends have focused on:
    ◦   Increased throughput
    ◦   Faster analysis time
    ◦   Quantitative resolution
    ◦   Ready to use reagents and assays – including probe and primer sequences to facilitate compliance with
        evolving MIQE guidelines


                                                       Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                      www.parioforma.com    2
PARIOFORMA




    ◦ Automation:
       Sample processing
       Liquid handling & filling
    − User friendly instrument operation:
       Software driven controls – “menu” driven devices
       Sophisticated data analysis software – integration with existing LIMs
   Future challenges will be to improve the down and upstream steps of real time PCR:
    ◦ Downstream:
       Reagents: quality, specificity and consistency
       Sample management and preparation
    ◦ Upstream:
       The challenge is to handle, annotate and analyse the very large amounts of data that will be generated.
       The implication is increasing sophistication of dedicated software for real-time PCR quality control, data
        management and analysis.




                                                       Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                      www.parioforma.com    3
PARIOFORMA




   For existing qRT-PCR users factors limiting the maximum use of the technology include:
     –   The cost of instrumentation and consumables.
     –   Machine access - more than 70% of scientists often share instrumentation with 10-12 other colleagues.


   Factors inhibiting the uptake of qRT-PCR by new users identified in interviews include:
     –   The perceived difficulty and complexity of running qRT-PCR assays
     –   Lack of experience in the technique
     –   Perceived cost of instrumentation and reagents – although existing methods take longer, they are cheaper to
         run
     –   Barriers to change existing working practices




                                                         Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                        www.parioforma.com    4
PARIOFORMA




   Early adopters include:                 Real time PCR is a mature technique but not
    Biotech & Pharma;                       a mature market
    Academia and Government
    research. These are well
    established in their use of




                                                                                                                        Saturation level
                                                                                                                        Saturation level
                                                                                                                        Saturation level
                                                                                                                        Saturation level
                                                                                                                        Saturation level
                                                                                                                        Saturation level
                                                                         GMO
    qRT-PCR with a large                                                                       Forensics
                                                                       Biodefense
    installed machine base.
                                                                                           Smaller food labs
                                                                     Large food Labs
   Late adopters include:                                                                    Veterinary
                                                             Clinical Labs
    forensics, veterinary                    Academia        Diagnostics
    diagnostics. These are just
                                                   Big       Large
    beginning to acquire             Roche       Pharma      CROs
    machines and develop new                       Biotech
                                      ABI
    methods.
   Others are somewhere in
    between – e.g. hospital &
    clinics; food & GMO testing;
    environment testing. These
    are expanding existing use     The diffusion of innovation technology With successive groups of consumers adopting
    of qRT-PCR into new areas.     the new technology (shown in blue), its market share (yellow) will eventually reach the
                                                                      saturation level.


                                                          Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                         www.parioforma.com                5
PARIOFORMA




     Primary application areas for qRT-PCR analyses are basic research and diagnostics (detecting the
      presence of target genetic material) – others are derivatives thereof:

                        Basic Research                                                  Diagnostics
    In research settings, qRT-PCR is mainly used to provide     Molecular diagnostics on the other hand, involves the
    quantitative measurements of gene transcription – or        measurement of DNA, RNA, proteins or metabolites to
    gene expression which is the process by which               detect genotypes, mutations or biochemical changes.
    information from a gene is used to synthesize a             Diagnostic qRT-PCR is applied to rapidly detect nucleic
    functional gene product (e.g. a protein).                   acids that are diagnostic of, for example, infectious
    The technology may be used in determining how the           diseases, cancer and genetic abnormalities –in humans,
    genetic expression of a particular gene changes over        plants and animals, etc.
    time, such as in the response of tissue and cell cultures   The introduction of qRT-PCR assays to the clinical
    to an administration of a pharmacological agent,            microbiology laboratory has significantly improved the
    progression of cell differentiation, or in response to      diagnosis of infectious diseases, and is deployed as a
    changes in environmental conditions.                        tool to detect newly emerging diseases, such as flu, in
    Gene expression profiling can help researchers              diagnostic tests.
    understand disease pathways which in turn helps them
    to identify means to interfere with those disease
    pathways (e.g. pharmacological) to develop treatments.
    Another important research application is genotyping.



                                                           Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                          www.parioforma.com    6
PARIOFORMA




THROUGHPUT
   The most widely used assay format is 96-well – “96-well” although not set as standard has become
    the industry standard across all applications.
   Below 96-well is considered “low throughput” – users in this sector are looking for flexibility in
    sample numbers per run – e.g. as provided by the Roche LightCycler® 2.0 Instrument (1 – 32
    samples per run)
   The second most widely used assay format was found to be 384-well pcr plate.

ASSAY VOLUME
   The majority of respondents were using a total assay volume range 10-25μl.
   A preference for 5μl of sample in a overall sample volume of 25μl was identified.
   The second most widely used assay volume range was found to be 25-50μl.
   End users in diagnostics (food, clinical, veterinary) all indicated a need for higher sensitivity and
    higher overall reaction volumes – in these applications precision and accuracy are paramount.



                                                    Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                   www.parioforma.com    7
PARIOFORMA




AUTOMATION
   Laboratories employ automation as a solution to achieve higher throughput and increased walk-
    away time – an automated solution must deliver improved productivity and workflow.
   Key needs for automated solutions:
    ◦   Cost-effective
    ◦   Fully-scalable
    ◦   Simplifies processes within the lab without compromising accuracy and precision
    ◦   Natural fit to lab work-flow (no added steps)
    ◦   Plug-and-play – software driven
    ◦   Bench-top
    ◦   Minimal manual reloading
   Interview findings suggest the end-users most well established in the use of automation are the
    early adopters of qRT-PCR (Pharma & Biotech R&D; Academia; Government research) – these have
    applied automation to many of their high throughput lines (>96 well).
   Adoption of automation outside of these segments is patchy and tends to follow how well
    established the lab is in using qRT-PCR (more frequent in larger labs than smaller ones).


                                                      Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                     www.parioforma.com    8
PARIOFORMA




REAGENTS
   The majority of respondents indicated a preference for pre-optimized qRT-PCR mastermixes (used
    according to manufacturers instructions) as opposed to qRT-PCR bulk reagents bought separately
    (optimized & validated in own lab).
   End-users want reagent systems that work first time without optimisation or validation.
   A large majority of consumables budget is spent on commercial kits – nevertheless, it must be
    clarified that end users are budget conscious. Many standard test kits are now available at very
    attractive prices while others (notably food and some diagnostic assays) command very high prices.
   End users tailor the use of commercial and in-house (home brew) assays to suit their budget and
    productivity needs:
      Sometimes it is more cost effective to buy a test (even if expensive) rather than develop and validate an in-
       house system (typical in large food labs);
      However, many large, busy clinical labs are now finding it more cost effective to develop in-house systems
       for high volume, routine analysis.




                                                       Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                      www.parioforma.com    9
PARIOFORMA




REAGENTS – immobilized systems
   The majority of Interviews revealed a high level of interest in lyophilized reagents:


                         Advantages                                            Disadvantages
       Convenient and time saving                             Expensive
       Easy to use                                            Potential for wastage
       De-skills end user staff requirements                  Storage and shelf-life concerns
       Proven to improve quality and reliability of the       End users dislike instrument systems with “tied”
       qRT-PCR reaction                                       assays



   It was reported that there is increasing demand for lyophilized systems – but the market remains
    embryonic with a limited number of suppliers which includes the large recognised names as well as
    emerging niche suppliers.




                                                          Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                         www.parioforma.com 10
PARIOFORMA




   Data quality expectation is the major buying selection criteria.
   Interview feedback indicated that end users buy “brands” – where the brand name conveys quality
    and confidence in instrument performance.
   Other important selection criteria included:
    ◦ Data analysis capability features
    ◦ Assay design
    ◦ HRM capability
    ◦ Running costs – cost of consumables
    ◦ Past experience
    ◦ Availability of training
   Instrument cost was cited as being less important overall than the aforementioned criteria.
   The instrument market is crowded – success depends on offering a quantum leap in features and
    benefits as perceived by the end-user:
    ◦    “It is very difficult for any newcomer to come to market with a “me-too” device and grab significant market
        share. Market penetration occurs by word of mouth – one lab has one and another will want it!” – Director of
        Research, Medical Genomics.


                                                       Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                      www.parioforma.com 11
PARIOFORMA



Parioforma Ltd. Is an independent business consultancy headquartered in London, UK.
Key benefits of working with Parioforma:
 Bespoke studies – tailored to client needs and
    timelines.
                                                                   LONDON OFFICE                      ASSOCIATE OFFICES
 Affordable research prices – makes your budgets go
    further.                                                                                                Intrix Corp, Darien
                                                              Charles Rowlands               USA
 High quality research – experienced science-based                                                         Connecticut
    team, all with post-graduate science and/ or MBA          Senior Partner
    qualifications and with 10-25+ years experience in Life                                  Eastern
    Science and High Technology industries.                   55 Prince’s Gate                              IMRP, Moscow
                                                                                             Europe
 Unique problem solving approach – the team is               Exhibition Road
    highly specialised, but diverse in experience and         London SW7 2PN                                Intrix Corp, São
    background, enabling analysis of problems from                                           Brazil
                                                              United Kingdom                                Paulo
    different perspectives.
                                                              Tel: +44 (0) 7803 907577
 Strategic Pointers – helps clients better understand        www.parioforma.com             Japan          TRN, Tokyo
    the research and implications for their business.
 Practical implementable solutions – not just
    “over-clever analysis.”                                   This is a sample report only. Please don't
Our knowledge is extensive and experience                     hesitate to contact us for an informal discussion
broad – please visit our website for a full                   on your market research needs – either by
listing of all our services.
                                                              telephone on +44 (0) 207 225 3538 or by email
                                                              at: charles.rowlands@parioforma.com
                                                              Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom
                                                                                                             www.parioforma.com 12

Más contenido relacionado

La actualidad más candente

Covid19 testing by rt pcr and rapid test kit , how it work
Covid19 testing by rt pcr and rapid test kit , how it workCovid19 testing by rt pcr and rapid test kit , how it work
Covid19 testing by rt pcr and rapid test kit , how it workVAISHNAVIGOBADE
 
Real time PCR
Real time PCRReal time PCR
Real time PCRnaren
 
Introduction to real-Time Quantitative PCR (qPCR) - Download the slides
Introduction to real-Time Quantitative PCR (qPCR) - Download the slidesIntroduction to real-Time Quantitative PCR (qPCR) - Download the slides
Introduction to real-Time Quantitative PCR (qPCR) - Download the slidesQIAGEN
 
Coronavirus test , rt pcr test, pcr technology, covid19 ,rapid test
Coronavirus test , rt pcr test, pcr technology, covid19 ,rapid testCoronavirus test , rt pcr test, pcr technology, covid19 ,rapid test
Coronavirus test , rt pcr test, pcr technology, covid19 ,rapid testAtoZBiology
 
Real-Time rt PCR, Dr mohamed ibrahim
Real-Time rt PCR, Dr mohamed ibrahimReal-Time rt PCR, Dr mohamed ibrahim
Real-Time rt PCR, Dr mohamed ibrahimMohamed Ibrahim Azzam
 
Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR): qPCR Technology Webinar S...
Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR): qPCR Technology Webinar S...Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR): qPCR Technology Webinar S...
Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR): qPCR Technology Webinar S...QIAGEN
 
An overview of hiv biology2 1.08.2011
An overview of hiv biology2 1.08.2011An overview of hiv biology2 1.08.2011
An overview of hiv biology2 1.08.2011Upasana Ganguly
 
RT PCR Protocol-creative biogene
RT PCR Protocol-creative biogeneRT PCR Protocol-creative biogene
RT PCR Protocol-creative biogeneDonglin Bao
 
Real time pcr applications-training-june 2010
Real time pcr applications-training-june 2010Real time pcr applications-training-june 2010
Real time pcr applications-training-june 2010Dr Dinesh Kumar
 
Real time pcr
Real time pcrReal time pcr
Real time pcrali h
 
Polymerase chain reaction (PCR)
Polymerase chain reaction (PCR)Polymerase chain reaction (PCR)
Polymerase chain reaction (PCR)shrihariprasad2
 
One Step Ahead for Your RT-PCR
One Step Ahead for Your RT-PCROne Step Ahead for Your RT-PCR
One Step Ahead for Your RT-PCRQIAGEN
 
chlamydia nucleic acid amplification tests
chlamydia nucleic acid amplification testschlamydia nucleic acid amplification tests
chlamydia nucleic acid amplification testsmorteza karami
 
Reverse transcriptase polymerase chain reaction
Reverse transcriptase polymerase chain reactionReverse transcriptase polymerase chain reaction
Reverse transcriptase polymerase chain reactionVidhi Doshi
 

La actualidad más candente (20)

Covid19 testing by rt pcr and rapid test kit , how it work
Covid19 testing by rt pcr and rapid test kit , how it workCovid19 testing by rt pcr and rapid test kit , how it work
Covid19 testing by rt pcr and rapid test kit , how it work
 
Real time PCR
Real time PCRReal time PCR
Real time PCR
 
Introduction to real-Time Quantitative PCR (qPCR) - Download the slides
Introduction to real-Time Quantitative PCR (qPCR) - Download the slidesIntroduction to real-Time Quantitative PCR (qPCR) - Download the slides
Introduction to real-Time Quantitative PCR (qPCR) - Download the slides
 
Coronavirus test , rt pcr test, pcr technology, covid19 ,rapid test
Coronavirus test , rt pcr test, pcr technology, covid19 ,rapid testCoronavirus test , rt pcr test, pcr technology, covid19 ,rapid test
Coronavirus test , rt pcr test, pcr technology, covid19 ,rapid test
 
RT PCR
RT PCRRT PCR
RT PCR
 
Real time PCR
Real time PCRReal time PCR
Real time PCR
 
Real-Time rt PCR, Dr mohamed ibrahim
Real-Time rt PCR, Dr mohamed ibrahimReal-Time rt PCR, Dr mohamed ibrahim
Real-Time rt PCR, Dr mohamed ibrahim
 
Real time pcr
Real time  pcrReal time  pcr
Real time pcr
 
Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR): qPCR Technology Webinar S...
Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR): qPCR Technology Webinar S...Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR): qPCR Technology Webinar S...
Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR): qPCR Technology Webinar S...
 
An overview of hiv biology2 1.08.2011
An overview of hiv biology2 1.08.2011An overview of hiv biology2 1.08.2011
An overview of hiv biology2 1.08.2011
 
RT PCR Protocol-creative biogene
RT PCR Protocol-creative biogeneRT PCR Protocol-creative biogene
RT PCR Protocol-creative biogene
 
Real time pcr applications-training-june 2010
Real time pcr applications-training-june 2010Real time pcr applications-training-june 2010
Real time pcr applications-training-june 2010
 
Real time pcr
Real time pcrReal time pcr
Real time pcr
 
Polymerase chain reaction (PCR)
Polymerase chain reaction (PCR)Polymerase chain reaction (PCR)
Polymerase chain reaction (PCR)
 
Nested pcr
Nested pcrNested pcr
Nested pcr
 
One Step Ahead for Your RT-PCR
One Step Ahead for Your RT-PCROne Step Ahead for Your RT-PCR
One Step Ahead for Your RT-PCR
 
Ppt.pcr appli.
Ppt.pcr appli.Ppt.pcr appli.
Ppt.pcr appli.
 
chlamydia nucleic acid amplification tests
chlamydia nucleic acid amplification testschlamydia nucleic acid amplification tests
chlamydia nucleic acid amplification tests
 
Variants of PCR
Variants of PCRVariants of PCR
Variants of PCR
 
Reverse transcriptase polymerase chain reaction
Reverse transcriptase polymerase chain reactionReverse transcriptase polymerase chain reaction
Reverse transcriptase polymerase chain reaction
 

Similar a Real time pcr market & end user needs survey

Webinar PCR - COVID-19, 2020
Webinar PCR - COVID-19, 2020Webinar PCR - COVID-19, 2020
Webinar PCR - COVID-19, 2020Sijo A
 
GeneMind Introduction.pdf
GeneMind Introduction.pdfGeneMind Introduction.pdf
GeneMind Introduction.pdfTylerNguyen40
 
Foregene's detection solution to covid 19
Foregene's detection solution to covid 19Foregene's detection solution to covid 19
Foregene's detection solution to covid 19Maggie Ma
 
RT PCR KIT For Covid 19 | Covid-19 RT PCR KIT
RT PCR KIT For Covid 19 | Covid-19 RT PCR KITRT PCR KIT For Covid 19 | Covid-19 RT PCR KIT
RT PCR KIT For Covid 19 | Covid-19 RT PCR KITADITYASAINI74
 
New Developments in Point of Care Diagnostics
New Developments in Point of Care DiagnosticsNew Developments in Point of Care Diagnostics
New Developments in Point of Care DiagnosticsBruce Carlson
 
RotorGene Q A Rapid, Automatable real-time PCR Instrument for Genotyping and...
RotorGene Q  A Rapid, Automatable real-time PCR Instrument for Genotyping and...RotorGene Q  A Rapid, Automatable real-time PCR Instrument for Genotyping and...
RotorGene Q A Rapid, Automatable real-time PCR Instrument for Genotyping and...QIAGEN
 
ResearchMoz - Polymerase chain reaction (pcr) in medical application an analy...
ResearchMoz - Polymerase chain reaction (pcr) in medical application an analy...ResearchMoz - Polymerase chain reaction (pcr) in medical application an analy...
ResearchMoz - Polymerase chain reaction (pcr) in medical application an analy...QYResearchReports
 
thermo fisher BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
thermo fisher  BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...thermo fisher  BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
thermo fisher BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...finance40
 
thermo fisher BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
thermo fisher  BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...thermo fisher  BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
thermo fisher BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...finance40
 
A Field-Deployable Insulated Isothermal PCR-Based System for Rapid and Sensit...
A Field-Deployable Insulated Isothermal PCR-Based System for Rapid and Sensit...A Field-Deployable Insulated Isothermal PCR-Based System for Rapid and Sensit...
A Field-Deployable Insulated Isothermal PCR-Based System for Rapid and Sensit...Simon Chung - genereach
 
Testing Concept.pdf
Testing Concept.pdfTesting Concept.pdf
Testing Concept.pdfssuser432659
 
Microbial Company
Microbial CompanyMicrobial Company
Microbial CompanyLaia Calvó
 
Investigate the Molecular Basis of Disease
Investigate the Molecular Basis of DiseaseInvestigate the Molecular Basis of Disease
Investigate the Molecular Basis of DiseaseQIAGEN
 
A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...
A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...
A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...Simon Chung - genereach
 

Similar a Real time pcr market & end user needs survey (20)

Webinar PCR - COVID-19, 2020
Webinar PCR - COVID-19, 2020Webinar PCR - COVID-19, 2020
Webinar PCR - COVID-19, 2020
 
GeneMind Introduction.pdf
GeneMind Introduction.pdfGeneMind Introduction.pdf
GeneMind Introduction.pdf
 
Foregene's detection solution to covid 19
Foregene's detection solution to covid 19Foregene's detection solution to covid 19
Foregene's detection solution to covid 19
 
RT PCR KIT For Covid 19 | Covid-19 RT PCR KIT
RT PCR KIT For Covid 19 | Covid-19 RT PCR KITRT PCR KIT For Covid 19 | Covid-19 RT PCR KIT
RT PCR KIT For Covid 19 | Covid-19 RT PCR KIT
 
New Developments in Point of Care Diagnostics
New Developments in Point of Care DiagnosticsNew Developments in Point of Care Diagnostics
New Developments in Point of Care Diagnostics
 
RotorGene Q A Rapid, Automatable real-time PCR Instrument for Genotyping and...
RotorGene Q  A Rapid, Automatable real-time PCR Instrument for Genotyping and...RotorGene Q  A Rapid, Automatable real-time PCR Instrument for Genotyping and...
RotorGene Q A Rapid, Automatable real-time PCR Instrument for Genotyping and...
 
ResearchMoz - Polymerase chain reaction (pcr) in medical application an analy...
ResearchMoz - Polymerase chain reaction (pcr) in medical application an analy...ResearchMoz - Polymerase chain reaction (pcr) in medical application an analy...
ResearchMoz - Polymerase chain reaction (pcr) in medical application an analy...
 
thermo fisher BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
thermo fisher  BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...thermo fisher  BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
thermo fisher BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
 
thermo fisher BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
thermo fisher  BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...thermo fisher  BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
thermo fisher BE83EA55-53AE-4224-AA31-E1B6E6FB41A4_UBSWestCoastLifeScienceBu...
 
A Field-Deployable Insulated Isothermal PCR-Based System for Rapid and Sensit...
A Field-Deployable Insulated Isothermal PCR-Based System for Rapid and Sensit...A Field-Deployable Insulated Isothermal PCR-Based System for Rapid and Sensit...
A Field-Deployable Insulated Isothermal PCR-Based System for Rapid and Sensit...
 
Testing Concept.pdf
Testing Concept.pdfTesting Concept.pdf
Testing Concept.pdf
 
Microbial Company
Microbial CompanyMicrobial Company
Microbial Company
 
Efrac drug brochure
Efrac drug brochureEfrac drug brochure
Efrac drug brochure
 
Introduction of RT PCR
Introduction of RT PCRIntroduction of RT PCR
Introduction of RT PCR
 
Investigate the Molecular Basis of Disease
Investigate the Molecular Basis of DiseaseInvestigate the Molecular Basis of Disease
Investigate the Molecular Basis of Disease
 
New EMA Guidance on Real Time Release Testing.
New EMA Guidance on Real Time Release Testing.New EMA Guidance on Real Time Release Testing.
New EMA Guidance on Real Time Release Testing.
 
Pcr array 2013
Pcr array 2013Pcr array 2013
Pcr array 2013
 
BioDuro
BioDuro BioDuro
BioDuro
 
pcr
pcrpcr
pcr
 
A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...
A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...
A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...
 

Más de Amy Morgan

Pricing strategy poland
Pricing strategy polandPricing strategy poland
Pricing strategy polandAmy Morgan
 
Hta in emerging markets
Hta in emerging marketsHta in emerging markets
Hta in emerging marketsAmy Morgan
 
The healthcare, pharmaceutical & clinical trial environment in russia
The healthcare, pharmaceutical & clinical trial environment in russia The healthcare, pharmaceutical & clinical trial environment in russia
The healthcare, pharmaceutical & clinical trial environment in russia Amy Morgan
 
Collaboration across the pharma enterprise
Collaboration across the pharma enterpriseCollaboration across the pharma enterprise
Collaboration across the pharma enterpriseAmy Morgan
 
Pharmaceutics landscape
Pharmaceutics landscapePharmaceutics landscape
Pharmaceutics landscapeAmy Morgan
 
HTA in emerging markets
HTA in emerging marketsHTA in emerging markets
HTA in emerging marketsAmy Morgan
 
Market access the challenges for medical devices
Market access   the challenges for medical devices Market access   the challenges for medical devices
Market access the challenges for medical devices Amy Morgan
 

Más de Amy Morgan (7)

Pricing strategy poland
Pricing strategy polandPricing strategy poland
Pricing strategy poland
 
Hta in emerging markets
Hta in emerging marketsHta in emerging markets
Hta in emerging markets
 
The healthcare, pharmaceutical & clinical trial environment in russia
The healthcare, pharmaceutical & clinical trial environment in russia The healthcare, pharmaceutical & clinical trial environment in russia
The healthcare, pharmaceutical & clinical trial environment in russia
 
Collaboration across the pharma enterprise
Collaboration across the pharma enterpriseCollaboration across the pharma enterprise
Collaboration across the pharma enterprise
 
Pharmaceutics landscape
Pharmaceutics landscapePharmaceutics landscape
Pharmaceutics landscape
 
HTA in emerging markets
HTA in emerging marketsHTA in emerging markets
HTA in emerging markets
 
Market access the challenges for medical devices
Market access   the challenges for medical devices Market access   the challenges for medical devices
Market access the challenges for medical devices
 

Último

Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024EwoutSteyerberg1
 
Neurological history taking (2024) .
Neurological  history  taking  (2024)  .Neurological  history  taking  (2024)  .
Neurological history taking (2024) .Mohamed Rizk Khodair
 
Pharmacokinetic Models by Dr. Ram D. Bawankar.ppt
Pharmacokinetic Models by Dr. Ram D.  Bawankar.pptPharmacokinetic Models by Dr. Ram D.  Bawankar.ppt
Pharmacokinetic Models by Dr. Ram D. Bawankar.pptRamDBawankar1
 
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptxDNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptxMAsifAhmad
 
power point presentation of Clinical evaluation of strabismus
power point presentation of Clinical evaluation  of strabismuspower point presentation of Clinical evaluation  of strabismus
power point presentation of Clinical evaluation of strabismusChandrasekar Reddy
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.whalesdesign
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionkrishnareddy157915
 
Adenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosisAdenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosisSujoy Dasgupta
 
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdfPAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdfDolisha Warbi
 
Red Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdfRed Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdfMedicoseAcademics
 
AUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functionsAUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functionsMedicoseAcademics
 
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.aarjukhadka22
 
"Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio..."Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio...Sujoy Dasgupta
 
AORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissectionAORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissectiondrhanifmohdali
 
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdfSGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdfHongBiThi1
 
Male Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy DasguptaMale Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy DasguptaSujoy Dasgupta
 
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...Shubhanshu Gaurav
 
How to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyHow to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyZurück zum Ursprung
 
Male Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and BeyondMale Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and BeyondSujoy Dasgupta
 

Último (20)

Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024Trustworthiness of AI based predictions Aachen 2024
Trustworthiness of AI based predictions Aachen 2024
 
Neurological history taking (2024) .
Neurological  history  taking  (2024)  .Neurological  history  taking  (2024)  .
Neurological history taking (2024) .
 
GOUT UPDATE AHMED YEHIA 2024, case based approach with application of the lat...
GOUT UPDATE AHMED YEHIA 2024, case based approach with application of the lat...GOUT UPDATE AHMED YEHIA 2024, case based approach with application of the lat...
GOUT UPDATE AHMED YEHIA 2024, case based approach with application of the lat...
 
Pharmacokinetic Models by Dr. Ram D. Bawankar.ppt
Pharmacokinetic Models by Dr. Ram D.  Bawankar.pptPharmacokinetic Models by Dr. Ram D.  Bawankar.ppt
Pharmacokinetic Models by Dr. Ram D. Bawankar.ppt
 
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptxDNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
DNA nucleotides Blast in NCBI and Phylogeny using MEGA Xi.pptx
 
power point presentation of Clinical evaluation of strabismus
power point presentation of Clinical evaluation  of strabismuspower point presentation of Clinical evaluation  of strabismus
power point presentation of Clinical evaluation of strabismus
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.
 
EXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung functionEXERCISE PERFORMANCE.pptx, Lung function
EXERCISE PERFORMANCE.pptx, Lung function
 
Adenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosisAdenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosis
 
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdfPAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
PAIN/CLASSIFICATION AND MANAGEMENT OF PAIN.pdf
 
Red Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdfRed Blood Cells_anemia & polycythemia.pdf
Red Blood Cells_anemia & polycythemia.pdf
 
AUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functionsAUTONOMIC NERVOUS SYSTEM organization and functions
AUTONOMIC NERVOUS SYSTEM organization and functions
 
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
Bulimia nervosa ( Eating Disorders) Mental Health Nursing.
 
"Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio..."Radical excision of DIE in subferile women with deep infiltrating endometrio...
"Radical excision of DIE in subferile women with deep infiltrating endometrio...
 
AORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissectionAORTIC DISSECTION and management of aortic dissection
AORTIC DISSECTION and management of aortic dissection
 
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdfSGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
 
Male Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy DasguptaMale Infertility Panel Discussion by Dr Sujoy Dasgupta
Male Infertility Panel Discussion by Dr Sujoy Dasgupta
 
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
FDMA FLAP - The first dorsal metacarpal artery (FDMA) flap is used mainly for...
 
How to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyHow to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturally
 
Male Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and BeyondMale Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and Beyond
 

Real time pcr market & end user needs survey

  • 1. PARIOFORMA Summary Report – September 2010 PARIOFORMA LTD 55 Princes Gate Exhibition Road London SW7 2PN United Kingdom Tel: +44 (0) 20 7225 3538 www.parioforma.com
  • 2. PARIOFORMA  Real-time PCR is becoming a mature technique.  Instruments are very robust and reliable, and optimised assays can be ordered from multiple vendors.  Although the technique will be further refined and more niche products will be developed, main future improvements are not expected within the qRT-PCR technology itself.  Growth drivers include: ◦ Increasingly robust assays ◦ Automation ◦ Increasing demand for high-throughput ◦ Multiplexed analytical strategies ◦ Increasingly affordable machines  Technology trends have focused on: ◦ Increased throughput ◦ Faster analysis time ◦ Quantitative resolution ◦ Ready to use reagents and assays – including probe and primer sequences to facilitate compliance with evolving MIQE guidelines Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 2
  • 3. PARIOFORMA ◦ Automation:  Sample processing  Liquid handling & filling − User friendly instrument operation:  Software driven controls – “menu” driven devices  Sophisticated data analysis software – integration with existing LIMs  Future challenges will be to improve the down and upstream steps of real time PCR: ◦ Downstream:  Reagents: quality, specificity and consistency  Sample management and preparation ◦ Upstream:  The challenge is to handle, annotate and analyse the very large amounts of data that will be generated.  The implication is increasing sophistication of dedicated software for real-time PCR quality control, data management and analysis. Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 3
  • 4. PARIOFORMA  For existing qRT-PCR users factors limiting the maximum use of the technology include: – The cost of instrumentation and consumables. – Machine access - more than 70% of scientists often share instrumentation with 10-12 other colleagues.  Factors inhibiting the uptake of qRT-PCR by new users identified in interviews include: – The perceived difficulty and complexity of running qRT-PCR assays – Lack of experience in the technique – Perceived cost of instrumentation and reagents – although existing methods take longer, they are cheaper to run – Barriers to change existing working practices Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 4
  • 5. PARIOFORMA  Early adopters include: Real time PCR is a mature technique but not Biotech & Pharma; a mature market Academia and Government research. These are well established in their use of Saturation level Saturation level Saturation level Saturation level Saturation level Saturation level GMO qRT-PCR with a large Forensics Biodefense installed machine base. Smaller food labs Large food Labs  Late adopters include: Veterinary Clinical Labs forensics, veterinary Academia Diagnostics diagnostics. These are just Big Large beginning to acquire Roche Pharma CROs machines and develop new Biotech ABI methods.  Others are somewhere in between – e.g. hospital & clinics; food & GMO testing; environment testing. These are expanding existing use The diffusion of innovation technology With successive groups of consumers adopting of qRT-PCR into new areas. the new technology (shown in blue), its market share (yellow) will eventually reach the saturation level. Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 5
  • 6. PARIOFORMA  Primary application areas for qRT-PCR analyses are basic research and diagnostics (detecting the presence of target genetic material) – others are derivatives thereof: Basic Research Diagnostics In research settings, qRT-PCR is mainly used to provide Molecular diagnostics on the other hand, involves the quantitative measurements of gene transcription – or measurement of DNA, RNA, proteins or metabolites to gene expression which is the process by which detect genotypes, mutations or biochemical changes. information from a gene is used to synthesize a Diagnostic qRT-PCR is applied to rapidly detect nucleic functional gene product (e.g. a protein). acids that are diagnostic of, for example, infectious The technology may be used in determining how the diseases, cancer and genetic abnormalities –in humans, genetic expression of a particular gene changes over plants and animals, etc. time, such as in the response of tissue and cell cultures The introduction of qRT-PCR assays to the clinical to an administration of a pharmacological agent, microbiology laboratory has significantly improved the progression of cell differentiation, or in response to diagnosis of infectious diseases, and is deployed as a changes in environmental conditions. tool to detect newly emerging diseases, such as flu, in Gene expression profiling can help researchers diagnostic tests. understand disease pathways which in turn helps them to identify means to interfere with those disease pathways (e.g. pharmacological) to develop treatments. Another important research application is genotyping. Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 6
  • 7. PARIOFORMA THROUGHPUT  The most widely used assay format is 96-well – “96-well” although not set as standard has become the industry standard across all applications.  Below 96-well is considered “low throughput” – users in this sector are looking for flexibility in sample numbers per run – e.g. as provided by the Roche LightCycler® 2.0 Instrument (1 – 32 samples per run)  The second most widely used assay format was found to be 384-well pcr plate. ASSAY VOLUME  The majority of respondents were using a total assay volume range 10-25μl.  A preference for 5μl of sample in a overall sample volume of 25μl was identified.  The second most widely used assay volume range was found to be 25-50μl.  End users in diagnostics (food, clinical, veterinary) all indicated a need for higher sensitivity and higher overall reaction volumes – in these applications precision and accuracy are paramount. Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 7
  • 8. PARIOFORMA AUTOMATION  Laboratories employ automation as a solution to achieve higher throughput and increased walk- away time – an automated solution must deliver improved productivity and workflow.  Key needs for automated solutions: ◦ Cost-effective ◦ Fully-scalable ◦ Simplifies processes within the lab without compromising accuracy and precision ◦ Natural fit to lab work-flow (no added steps) ◦ Plug-and-play – software driven ◦ Bench-top ◦ Minimal manual reloading  Interview findings suggest the end-users most well established in the use of automation are the early adopters of qRT-PCR (Pharma & Biotech R&D; Academia; Government research) – these have applied automation to many of their high throughput lines (>96 well).  Adoption of automation outside of these segments is patchy and tends to follow how well established the lab is in using qRT-PCR (more frequent in larger labs than smaller ones). Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 8
  • 9. PARIOFORMA REAGENTS  The majority of respondents indicated a preference for pre-optimized qRT-PCR mastermixes (used according to manufacturers instructions) as opposed to qRT-PCR bulk reagents bought separately (optimized & validated in own lab).  End-users want reagent systems that work first time without optimisation or validation.  A large majority of consumables budget is spent on commercial kits – nevertheless, it must be clarified that end users are budget conscious. Many standard test kits are now available at very attractive prices while others (notably food and some diagnostic assays) command very high prices.  End users tailor the use of commercial and in-house (home brew) assays to suit their budget and productivity needs:  Sometimes it is more cost effective to buy a test (even if expensive) rather than develop and validate an in- house system (typical in large food labs);  However, many large, busy clinical labs are now finding it more cost effective to develop in-house systems for high volume, routine analysis. Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 9
  • 10. PARIOFORMA REAGENTS – immobilized systems  The majority of Interviews revealed a high level of interest in lyophilized reagents: Advantages Disadvantages Convenient and time saving Expensive Easy to use Potential for wastage De-skills end user staff requirements Storage and shelf-life concerns Proven to improve quality and reliability of the End users dislike instrument systems with “tied” qRT-PCR reaction assays  It was reported that there is increasing demand for lyophilized systems – but the market remains embryonic with a limited number of suppliers which includes the large recognised names as well as emerging niche suppliers. Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 10
  • 11. PARIOFORMA  Data quality expectation is the major buying selection criteria.  Interview feedback indicated that end users buy “brands” – where the brand name conveys quality and confidence in instrument performance.  Other important selection criteria included: ◦ Data analysis capability features ◦ Assay design ◦ HRM capability ◦ Running costs – cost of consumables ◦ Past experience ◦ Availability of training  Instrument cost was cited as being less important overall than the aforementioned criteria.  The instrument market is crowded – success depends on offering a quantum leap in features and benefits as perceived by the end-user: ◦ “It is very difficult for any newcomer to come to market with a “me-too” device and grab significant market share. Market penetration occurs by word of mouth – one lab has one and another will want it!” – Director of Research, Medical Genomics. Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 11
  • 12. PARIOFORMA Parioforma Ltd. Is an independent business consultancy headquartered in London, UK. Key benefits of working with Parioforma:  Bespoke studies – tailored to client needs and timelines. LONDON OFFICE ASSOCIATE OFFICES  Affordable research prices – makes your budgets go further. Intrix Corp, Darien Charles Rowlands USA  High quality research – experienced science-based Connecticut team, all with post-graduate science and/ or MBA Senior Partner qualifications and with 10-25+ years experience in Life Eastern Science and High Technology industries. 55 Prince’s Gate IMRP, Moscow Europe  Unique problem solving approach – the team is Exhibition Road highly specialised, but diverse in experience and London SW7 2PN Intrix Corp, São background, enabling analysis of problems from Brazil United Kingdom Paulo different perspectives. Tel: +44 (0) 7803 907577  Strategic Pointers – helps clients better understand www.parioforma.com Japan TRN, Tokyo the research and implications for their business.  Practical implementable solutions – not just “over-clever analysis.” This is a sample report only. Please don't Our knowledge is extensive and experience hesitate to contact us for an informal discussion broad – please visit our website for a full on your market research needs – either by listing of all our services. telephone on +44 (0) 207 225 3538 or by email at: charles.rowlands@parioforma.com Parioforma Ltd – 55 Princes Gate – London SW7 2PN – United Kingdom www.parioforma.com 12