1. The document discusses filariasis, caused by parasitic nematodes Wuchereria bancrofti and Brugia malayi. The parasites reside in lymphatic vessels and blood, with motile microfilariae circulating in the blood and skin. 2. Symptoms of filariasis infection include hydrocele, lymphedema, and elephantiasis resulting from inflammatory responses and lymphatic damage. Diagnosis involves microscopic examination of blood smears and hydrocele fluid to identify the microfilarial worms. 3. Various laboratory techniques for detection of microfilariae are described, including thick blood smears, membrane filtration, and concentration methods like Knott's technique and

1. ASPEK LABORATORIUM KLINIK FILARIASIS Tinjauan Pustaka Mikrobiologi Hermi Indita / Prihatini 1

2. 2

3. 3

4. Patogenesis Siklus Hidup 4

5. Gambar 2. Siklus hidup Filaria 6 5

7. Infeksi berulang obstruksi getah bening menetap edema, fibrosis & obstruksi elefantiasis 6

9. Terjadi infeksi sekunder akibat jamur & bakteri

10. Didalam tubuh cacing dewasa W.bancrofti terdapat bakteri simbion genus Wolbachia.

11. Wolbachia mempunyai efek antihelmintik & molekul mirip endotoksin yg berperan pada respon inflamasi pada cacing mati 7

12. Gejala Klinis 8

13. 9

14. Gambar 4. Penderita positif W.bancrofti dengan filarial abscess scar. 8 Gambar 3. Penderita positif W bancrofti dengan hidrokel bilateral, pembesaran testikular dan lymphadenopathy inguinal. 8 Gambar 5 A. Penderita elefantiasis bilateral 10 Gambar 5 B. Penderita elefantiasis dekstra 10 10

15. Diagnosis 11

16. Pemeriksaan konsentrasi Hasil 12

17. Metode konsentrasi Knott 13

18. Sediaan darah tebal segar Sediaan darah tebal kering 14

19. Sediaan darah tipis 15

20. Tehnik Filtrasi Membran 16

21. Tehnik QBC (Quantitative Buffy Coat Technique) 17

22. 18

23. 19

24. TERIMA KASIH 20

26. Clumps of RBC (normal in thick part of film).

28. Clumps of RBC (normal in thick part of film).

30. Head part

32. Dark blue structures are nuclei

33. Tail end tapering (no nuclei)

35. Head end of the worm – rounded (no nuclei)

37. Dark blue structures are nuclei

40. Inflammatory cells – lymphocytes.

42. Inflammatory cells – lymphocytes.

44. Inflammatory cells – lymphocytes.

46. RBC

48. Discussion: Filariasis Filaria infesting humans: Wuchereria bancrofti – Elephantiasis Brugia malayi – milder form, lymphatics. Onchocerca volvulus – Eye, blindness, Africa Loa Loa – Loaisis, Africa, repeated swellings. Mansonella perstans – Africa, asymptomatic. 33

49. Diperiksadi bawah lensaobyektifkhusus (paralens) berkekuatan 60X ygdihubungkan fiber optik Sampel darah dimasukkankedalam tabungkapilermengandung AO & anti koagulan Tabungkapilerdisentrifuse Cara Pemeriksaan 34

50. 35

51. Morphology of the microfilaria (mf) 36

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58. 43