Slides on experimental methods for Developmental Biology, spring 2012

1. Experimental
Techniques

2. Chapters 3 and 5

3. Chapter 10

4. Gene Cloning

5. Figure 10-13 Essential Cell Biology (© Garland Science 2010)

6. Figure 10-14 Essential Cell Biology (© Garland Science 2010)

7. Polymerase Chain Reaction
(PCR)

8. Figure 10-15 Essential Cell Biology (© Garland Science 2010)

9. Figure 10-16 Essential Cell Biology (© Garland Science 2010)

10. Figure 10-17 Essential Cell Biology (© Garland Science 2010)

11. Mutagenesis

15. Figure 10-34 (part 1 of 2) Essential Cell Biology (© Garland Science 2010)

16. Figure 10-34 (part 2 of 2) Essential Cell Biology (© Garland Science 2010)

18. Gene Knock-Out

19. Figure 10-35 Essential Cell Biology (© Garland Science 2010)

22. Gene Replacement

23. Figure 10-35 Essential Cell Biology (© Garland Science 2010)

24. Reporter Genes

25. Figure 10-29a Essential Cell Biology (© Garland Science 2010)

26. Figure 10-30 Essential Cell Biology (© Garland Science 2010)

27. Transgenesis

28. Figure 10-36 (part 1 of 2) Essential Cell Biology (© Garland Science 2010)

29. Figure 10-36 (part 2 of 2) Essential Cell Biology (© Garland Science 2010)

30. Figure 10-37 Essential Cell Biology (© Garland Science 2010)

31. RNAi

32. microRNA (miRNA)
Short, noncoding, regulatory
RNAs controlling gene expression

33. Figure 8-26 Essential Cell Biology (© Garland Science 2010)

34. small interfering RNA (siRNA)
Short, double-stranded
RNAs controlling gene expression

35. Figure 8-27 Essential Cell Biology (© Garland Science 2010)

36. Figure 10-38a Essential Cell Biology (© Garland Science 2010)

37. Figure 10-38b,c Essential Cell Biology (© Garland Science 2010)

38. Nucleic Acid
Hybridization

39. Figure 10-31 Essential Cell Biology (© Garland Science 2010)

41. DNA Microarrays

42. Figure 10-33 (part 1 of 2) Essential Cell Biology (© Garland Science 2010)

43. Figure 10-33 (part 2 of 2) Essential Cell Biology (© Garland Science 2010)

44. Blotting

45. Figure 10-5 Essential Cell Biology (© Garland Science 2010)

46. Figure 10-5 (part 1 of 5) Essential Cell Biology (© Garland Science 2010)

47. Gel Electrophoresis

48. Figure 10-3 Essential Cell Biology (© Garland Science 2010)

49. Figure 10-5 (part 2 of 5) Essential Cell Biology (© Garland Science 2010)

50. Figure 10-5 (part 3 of 5) Essential Cell Biology (© Garland Science 2010)

51. Figure 10-5 (part 4 of 5) Essential Cell Biology (© Garland Science 2010)

52. Figure 10-5 (part 5 of 5) Essential Cell Biology (© Garland Science 2010)

53. Figure 10-5 Essential Cell Biology (© Garland Science 2010)

54. Immunostaining

56. Fusion Proteins