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RNA AND  PROTEIN SYNTHESIS By  Muhammad Asif Pu Pak Muhammad Asif Pu
How your cell makes very important proteins ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Muhammad Asif Pu
DNA     RNA     Protein Eukaryotic Cell Muhammad Asif Pu Nuclear membrane Transcription RNA Processing Translation DNA Pre-mRNA mRNA Ribosome Protein
Before making proteins, Your cell must first make RNA   ,[object Object],[object Object],Muhammad Asif Pu
RNA  differs from  DNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Muhammad Asif Pu
Muhammad Asif Pu
1.  Transcription Eukaryotic Cell Muhammad Asif Pu Nuclear membrane Transcription RNA Processing Translation DNA Pre-mRNA mRNA Ribosome Protein
1. Transcription OR  RNA production ,[object Object],[object Object],Muhammad Asif Pu
1.  Transcription Muhammad Asif Pu DNA pre-mRNA RNA Polymerase
Question: ,[object Object],[object Object],Muhammad Asif Pu
Types of RNA ,[object Object],[object Object],[object Object],[object Object],[object Object],Muhammad Asif Pu
mRNA ,[object Object],[object Object],[object Object],Muhammad Asif Pu
A. Messenger RNA (mRNA) Muhammad Asif Pu methionine glycine serine isoleucine glycine alanine stop codon protein A U G G G C U C C A U C G G C G C A U A A mRNA start codon Primary structure of a protein aa1 aa2 aa3 aa4 aa5 aa6 peptide bonds codon 2 codon 3 codon 4 codon 5 codon 6 codon 7 codon 1
If the cell is a school… ,[object Object],[object Object],[object Object],[object Object],[object Object],Muhammad Asif Pu
rRNA ,[object Object],[object Object],tRNA A go-getter.  Gets the right parts to make the right protein according to mRNA instructions Muhammad Asif Pu
B. Transfer RNA (tRNA) Muhammad Asif Pu amino acid attachment site U A C anticodon methionine amino acid
2. RNA Processing Eukaryotic Cell Muhammad Asif Pu Nuclear membrane Transcription RNA Processing Translation DNA Pre-mRNA mRNA Ribosome Protein
2. RNA Processing ,[object Object],[object Object],[object Object],Muhammad Asif Pu
2. RNA Processing Muhammad Asif Pu pre-RNA molecule intron intron exon exon exon exon exon exon Mature RNA molecule exon exon exon intron intron splicesome splicesome
Ribosomes P Site A Site Large subunit Small subunit Muhammad Asif Pu mRNA A U G C U A C U U C G
3. Translation - making proteins Eukaryotic Cell Muhammad Asif Pu Nuclear membrane Transcription RNA Processing Translation DNA Pre-mRNA mRNA Ribosome Protein
3. Translation ,[object Object],[object Object],[object Object],[object Object],[object Object],Muhammad Asif Pu
3. Translation P Site A Site Large subunit Small subunit Muhammad Asif Pu mRNA A U G C U A C U U C G
Initiation mRNA A U G C U A C U U C G A anticodon hydrogen bonds codon Muhammad Asif Pu 2-tRNA G aa2 A U 1-tRNA U A C aa1
mRNA A U G C U A C U U C G 1-tRNA 2-tRNA U A C G aa1 aa2 A U A anticodon hydrogen bonds codon peptide bond Elongation Muhammad Asif Pu 3-tRNA G A A aa3
mRNA A U G C U A C U U C G 1-tRNA 2-tRNA U A C G aa1 aa2 A U A peptide bond Ribosomes move over one codon (leaves) Muhammad Asif Pu 3-tRNA G A A aa3
mRNA A U G C U A C U U C G 2-tRNA G aa1 aa2 A U A peptide bonds 3-tRNA G A A aa3 A C U Muhammad Asif Pu 4-tRNA G C U aa4
mRNA A U G C U A C U U C G 2-tRNA G aa1 aa2 A U A peptide bonds 3-tRNA G A A aa3 A C U (leaves) Ribosomes move over one codon Muhammad Asif Pu 4-tRNA G C U aa4
mRNA G C U A C U U C G aa1 aa2 A peptide bonds 3-tRNA G A A aa3 4-tRNA G C U aa4 A C U Muhammad Asif Pu U G A 5-tRNA aa5
mRNA G C U A C U U C G aa1 aa2 A peptide bonds 3-tRNA G A A aa3 4-tRNA G C U aa4 A C U Ribosomes move over one codon Muhammad Asif Pu U G A 5-tRNA aa5
mRNA A C A U G U aa1 aa2 U primary structure of a protein aa3 200-tRNA aa4 U A G aa5 C U aa200 aa199 terminator or stop codon Termination Muhammad Asif Pu
End Product ,[object Object],[object Object],Muhammad Asif Pu aa1 aa2 aa3 aa4 aa5 aa200 aa199
Question: ,[object Object],[object Object],Muhammad Asif Pu
Answer: ,[object Object],[object Object],[object Object],Muhammad Asif Pu

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Rna and protein synthesis

  • 1. RNA AND PROTEIN SYNTHESIS By Muhammad Asif Pu Pak Muhammad Asif Pu
  • 2.
  • 3. DNA  RNA  Protein Eukaryotic Cell Muhammad Asif Pu Nuclear membrane Transcription RNA Processing Translation DNA Pre-mRNA mRNA Ribosome Protein
  • 4.
  • 5.
  • 7. 1. Transcription Eukaryotic Cell Muhammad Asif Pu Nuclear membrane Transcription RNA Processing Translation DNA Pre-mRNA mRNA Ribosome Protein
  • 8.
  • 9. 1. Transcription Muhammad Asif Pu DNA pre-mRNA RNA Polymerase
  • 10.
  • 11.
  • 12.
  • 13. A. Messenger RNA (mRNA) Muhammad Asif Pu methionine glycine serine isoleucine glycine alanine stop codon protein A U G G G C U C C A U C G G C G C A U A A mRNA start codon Primary structure of a protein aa1 aa2 aa3 aa4 aa5 aa6 peptide bonds codon 2 codon 3 codon 4 codon 5 codon 6 codon 7 codon 1
  • 14.
  • 15.
  • 16. B. Transfer RNA (tRNA) Muhammad Asif Pu amino acid attachment site U A C anticodon methionine amino acid
  • 17. 2. RNA Processing Eukaryotic Cell Muhammad Asif Pu Nuclear membrane Transcription RNA Processing Translation DNA Pre-mRNA mRNA Ribosome Protein
  • 18.
  • 19. 2. RNA Processing Muhammad Asif Pu pre-RNA molecule intron intron exon exon exon exon exon exon Mature RNA molecule exon exon exon intron intron splicesome splicesome
  • 20. Ribosomes P Site A Site Large subunit Small subunit Muhammad Asif Pu mRNA A U G C U A C U U C G
  • 21. 3. Translation - making proteins Eukaryotic Cell Muhammad Asif Pu Nuclear membrane Transcription RNA Processing Translation DNA Pre-mRNA mRNA Ribosome Protein
  • 22.
  • 23. 3. Translation P Site A Site Large subunit Small subunit Muhammad Asif Pu mRNA A U G C U A C U U C G
  • 24. Initiation mRNA A U G C U A C U U C G A anticodon hydrogen bonds codon Muhammad Asif Pu 2-tRNA G aa2 A U 1-tRNA U A C aa1
  • 25. mRNA A U G C U A C U U C G 1-tRNA 2-tRNA U A C G aa1 aa2 A U A anticodon hydrogen bonds codon peptide bond Elongation Muhammad Asif Pu 3-tRNA G A A aa3
  • 26. mRNA A U G C U A C U U C G 1-tRNA 2-tRNA U A C G aa1 aa2 A U A peptide bond Ribosomes move over one codon (leaves) Muhammad Asif Pu 3-tRNA G A A aa3
  • 27. mRNA A U G C U A C U U C G 2-tRNA G aa1 aa2 A U A peptide bonds 3-tRNA G A A aa3 A C U Muhammad Asif Pu 4-tRNA G C U aa4
  • 28. mRNA A U G C U A C U U C G 2-tRNA G aa1 aa2 A U A peptide bonds 3-tRNA G A A aa3 A C U (leaves) Ribosomes move over one codon Muhammad Asif Pu 4-tRNA G C U aa4
  • 29. mRNA G C U A C U U C G aa1 aa2 A peptide bonds 3-tRNA G A A aa3 4-tRNA G C U aa4 A C U Muhammad Asif Pu U G A 5-tRNA aa5
  • 30. mRNA G C U A C U U C G aa1 aa2 A peptide bonds 3-tRNA G A A aa3 4-tRNA G C U aa4 A C U Ribosomes move over one codon Muhammad Asif Pu U G A 5-tRNA aa5
  • 31. mRNA A C A U G U aa1 aa2 U primary structure of a protein aa3 200-tRNA aa4 U A G aa5 C U aa200 aa199 terminator or stop codon Termination Muhammad Asif Pu
  • 32.
  • 33.
  • 34.