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Breeding	
  for	
  rust	
  
resistance	
  in	
  Kenya:	
  
Successes,	
  setbacks	
  and	
  
future	
  approaches	
  
	
  
P.Njau, R. Wanyera, S. Bhavani, L. Karani and
M. Gethi
njaupnn@yahoo.com
•  “Ug99”	
  –	
  first	
  
reported	
  in	
  Uganda	
  
1998/9.	
  
•  Muta9ng	
  and	
  
migra9ng	
  (2012:	
  8	
  
races	
  iden9fied	
  in	
  
group,	
  present	
  in	
  11	
  
countries)	
  	
  
•  Race	
  TTKSK	
  (original	
  
“Ug99”	
  [red])	
  only	
  
predominates	
  in	
  
Ethiopia	
  
•  Other	
  Ug99	
  race	
  
group	
  races	
  
predominate	
  e.g.,	
  
Sr24	
  variants	
  in	
  Kenya	
  
Ug99	
  a	
  challenging	
  race	
  to	
  beat	
  
The	
  cri9cal	
  screening	
  facility	
  in	
  Njoro	
  
1.  12	
  Hectares	
  of	
  land	
  available	
  
for	
  screening	
  
2.  Irriga9on	
  facility	
  
3.  Greenhouse	
  underway	
  
4.  Trained	
  personnel	
  
5.  Room	
  for	
  expansion	
  available	
  
• Over	
  300,000	
  accessions	
  have	
  been	
  
evaluated	
  so	
  far	
  
• Ability	
  to	
  evaluate	
  50,000	
  accessions	
  
every	
  year	
  
• Winter	
  wheat	
  are	
  vernalized	
  and	
  
screened	
  in	
  the	
  field	
  
Breeding	
  for	
  Resistance	
  
New	
  mutants	
  of	
  the	
  Ug99	
  race	
  easily	
  overcome	
  
the	
  deployed	
  genes	
  as	
  was	
  experienced	
  in	
  2007	
  
	
  
Na9onal	
  Breeding	
  Programme	
  plays	
  an	
  
important	
  role	
  in	
  incorpora9ng	
  the	
  resistant	
  
gene	
  in	
  the	
  commercial	
  varie9es	
  
Mexico (Cd. Obregon-Toluca/El Batan)- Kenya International Shuttle
Breeding:
a five-year breeding cycle)
Cd. Obregón 39 masl
High yield (irrigated), Water-use
efficiency, Heat tolerance, Leaf rust, stem
rust (not Ug99)
Toluca 2640
masl
Yellow rust
Septoria tritici
Fusarium
Zero tillage
El Batán 2249
masl
Leaf rust,
Fusarium
Njoro, Kenya 2185
masl
Stem rust (Ug99
group)
Yellow rust
●  F1 and F1top/backcross developed at CIMMYT, and
advanced to F2.
●  F3 and F4/F5 evaluated in Njoro.
●  Advanced F6 and initial observation in Mexico
●  Observation and selection in Njoro where lines with
suitable adaptation are retained
Varie9es	
  developed	
  
Commercial	
  Name	
   Pedigree	
  
Kenya Wren	
   THELIN # 2/TUKURU	
  
Robin	
   BABAX/LR42//BABAX*2/3/TUKURU	
  
Kenya Tai	
   ND643/2*WBLL1	
  
Kenya Sunbird	
   ND643/2*WBLL1	
  
Eagle10	
   EMB 16/CBRD//CBRD	
  
Kenya Korongo	
   BABAX/LR42//BABAX*2/4/SNI/TRAP#1/3/KAUZ*2/TRAP//
KAUZ	
  
Kenya Kingbird	
   TAM200/TUI/6/PVN//CAR422/ANA/5/BOW/CROW//BUC/
PVN/3/YR/4/TRAP#1	
  
Kenya Hawk12	
   URES/JUN//KAUZ/3/BABAX/4/TILHI	
  
The	
  Seed	
  System	
  and	
  seed	
  
produc9on	
  
Our	
  focus	
  
•  In	
  future,	
  breeding	
  for	
  rust	
  resistance	
  will	
  focus	
  
on	
  combining	
  both	
  major	
  and	
  minor	
  genes	
  for	
  
maximum	
  protec9on	
  and	
  durability.	
  	
  
•  The	
  use	
  of	
  molecular	
  markers	
  to	
  increase	
  
efficiency	
  will	
  aid	
  selec9on	
  of	
  complex	
  
resistances.	
  	
  
•  KARI	
  is	
  collabora9ng	
  with	
  CIMMYT	
  and	
  Na9onal	
  
Ins9tute	
  of	
  Agricultural	
  Botany	
  (NIAB)	
  in	
  a	
  BBSRC	
  
funded	
  project	
  to	
  improve	
  molecular	
  laboratory	
  
facili9es	
  in	
  Kenya	
  so	
  as	
  to	
  implement	
  marker	
  
assisted	
  breeding	
  strategies	
  
Training	
  and	
  capacity	
  building	
  
Training	
   course	
   organized	
   at	
   KARI	
   every	
   year	
  
under	
   the	
   DRRW	
   project	
   are	
   designed	
   to	
   train	
  
wheat	
  breeders	
  from	
  public	
  and	
  private	
  sector	
  
in	
  Africa,	
  the	
  Middle	
  East	
  and	
  Central	
  and	
  South	
  
Asia,	
   who	
   wish	
   to	
   learn	
   about	
   stem	
   rust,	
  
evalua9on	
   of	
   germplasm,	
   and	
   standardiza9on	
  
of	
  note	
  taking,	
  as	
  well	
  as	
  to	
  update	
  themselves	
  
with	
   the	
   global	
   knowledge	
   and	
   innova9ve	
  
techniques	
   that	
   can	
   enhance	
   progress	
   and	
  
efficiency	
  in	
  their	
  breeding	
  ac9vi9es.	
  
This work is funded by the BMGF through Cornel
University under the DRRW project
Need	
  to	
  play	
  a	
  regional	
  role	
  
• Training	
  
• Breeding/selec9on	
  
• Breeder	
  seed	
  and	
  cer9fied	
  seed	
  produc9on	
  
and	
  distribu9on	
  
• Screening	
  of	
  regional	
  advanced	
  wheat	
  lines	
  
	
  

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Breeding for Rust Resistance in Kenya: Successes, Setbacks, and Future Approaches

  • 1. Breeding  for  rust   resistance  in  Kenya:   Successes,  setbacks  and   future  approaches     P.Njau, R. Wanyera, S. Bhavani, L. Karani and M. Gethi njaupnn@yahoo.com
  • 2. •  “Ug99”  –  first   reported  in  Uganda   1998/9.   •  Muta9ng  and   migra9ng  (2012:  8   races  iden9fied  in   group,  present  in  11   countries)     •  Race  TTKSK  (original   “Ug99”  [red])  only   predominates  in   Ethiopia   •  Other  Ug99  race   group  races   predominate  e.g.,   Sr24  variants  in  Kenya   Ug99  a  challenging  race  to  beat  
  • 3. The  cri9cal  screening  facility  in  Njoro   1.  12  Hectares  of  land  available   for  screening   2.  Irriga9on  facility   3.  Greenhouse  underway   4.  Trained  personnel   5.  Room  for  expansion  available   • Over  300,000  accessions  have  been   evaluated  so  far   • Ability  to  evaluate  50,000  accessions   every  year   • Winter  wheat  are  vernalized  and   screened  in  the  field  
  • 4. Breeding  for  Resistance   New  mutants  of  the  Ug99  race  easily  overcome   the  deployed  genes  as  was  experienced  in  2007     Na9onal  Breeding  Programme  plays  an   important  role  in  incorpora9ng  the  resistant   gene  in  the  commercial  varie9es  
  • 5. Mexico (Cd. Obregon-Toluca/El Batan)- Kenya International Shuttle Breeding: a five-year breeding cycle) Cd. Obregón 39 masl High yield (irrigated), Water-use efficiency, Heat tolerance, Leaf rust, stem rust (not Ug99) Toluca 2640 masl Yellow rust Septoria tritici Fusarium Zero tillage El Batán 2249 masl Leaf rust, Fusarium Njoro, Kenya 2185 masl Stem rust (Ug99 group) Yellow rust ●  F1 and F1top/backcross developed at CIMMYT, and advanced to F2. ●  F3 and F4/F5 evaluated in Njoro. ●  Advanced F6 and initial observation in Mexico ●  Observation and selection in Njoro where lines with suitable adaptation are retained
  • 6. Varie9es  developed   Commercial  Name   Pedigree   Kenya Wren   THELIN # 2/TUKURU   Robin   BABAX/LR42//BABAX*2/3/TUKURU   Kenya Tai   ND643/2*WBLL1   Kenya Sunbird   ND643/2*WBLL1   Eagle10   EMB 16/CBRD//CBRD   Kenya Korongo   BABAX/LR42//BABAX*2/4/SNI/TRAP#1/3/KAUZ*2/TRAP// KAUZ   Kenya Kingbird   TAM200/TUI/6/PVN//CAR422/ANA/5/BOW/CROW//BUC/ PVN/3/YR/4/TRAP#1   Kenya Hawk12   URES/JUN//KAUZ/3/BABAX/4/TILHI  
  • 7. The  Seed  System  and  seed   produc9on  
  • 8. Our  focus   •  In  future,  breeding  for  rust  resistance  will  focus   on  combining  both  major  and  minor  genes  for   maximum  protec9on  and  durability.     •  The  use  of  molecular  markers  to  increase   efficiency  will  aid  selec9on  of  complex   resistances.     •  KARI  is  collabora9ng  with  CIMMYT  and  Na9onal   Ins9tute  of  Agricultural  Botany  (NIAB)  in  a  BBSRC   funded  project  to  improve  molecular  laboratory   facili9es  in  Kenya  so  as  to  implement  marker   assisted  breeding  strategies  
  • 9. Training  and  capacity  building   Training   course   organized   at   KARI   every   year   under   the   DRRW   project   are   designed   to   train   wheat  breeders  from  public  and  private  sector   in  Africa,  the  Middle  East  and  Central  and  South   Asia,   who   wish   to   learn   about   stem   rust,   evalua9on   of   germplasm,   and   standardiza9on   of  note  taking,  as  well  as  to  update  themselves   with   the   global   knowledge   and   innova9ve   techniques   that   can   enhance   progress   and   efficiency  in  their  breeding  ac9vi9es.  
  • 10. This work is funded by the BMGF through Cornel University under the DRRW project Need  to  play  a  regional  role   • Training   • Breeding/selec9on   • Breeder  seed  and  cer9fied  seed  produc9on   and  distribu9on   • Screening  of  regional  advanced  wheat  lines