Stem Cell Preservation in Surat

1. Cryo-save India (part of Cryo-Save NV) Stem cells Banking

3. What are sources/types of stem cells in the Human Body? 3.Blood Stem Cells 1.Bone Marrow Stem Cells 2.Embryonic Stem cells 4.Cord Lining Stem Cells

4. Cryo-save India (part of Cryo-Save NV) Why stem cells from U cord After a baby is born and the umbilical cord is cut, some blood remains in the blood vessels of the placenta and the portion of the umbilical cord that remains attached to it. After birth, the baby no longer needs this extra blood. This blood is called placental blood or umbilical cord blood: "cord blood" for short. Cord blood contains all the normal elements of blood - red blood cells, white blood cells, platelets and plasma. But it is also rich in hematopoietic (blood-forming) stem cells, similar to those found in bone marrow. This is why cord blood can be used for transplantation instead of bone marrow. Cord blood is being used increasingly on an experimental basis as a source of stem cells, as an alternative to bone marrow. Most cord blood transplants have been done to treat diseases of the blood and immune system. It has also been used to restore the functional deficiencies of several genetic metabolic diseases. To date, more than 70 different diseases have been treated with cord blood transplants.

5. bone marrow cord blood Donation requires surgery under general anesthesia. Donors may experience temporary discomfort and/or pain. Long-term consequences of growth factors used in peripheral blood stem cell donations are uncertain. When obtained from the delivered placenta and umbilical cord, donation poses no risk to mother or infant. Requires donation of a quart or more of bone marrow (mixed with blood) for transplant A few ounces can be used for transplantation. Large dose of stem cells. Rapid engraftment. Smaller dose of stem cells. Slower engraftment Potential donors may no longer be available or may have withdrawn consent. Donor must be found and retested to confirm the HLA typing and infectious disease results and to confirm that the donor is still willing and able to donate bone marrow. Significant donor attrition Once frozen, a cord blood unit is available until used. Otherwise, no donor attrition Generally requires a perfect match between donor and recipient for 6/6 HLA Generally requires a match between donor and recipient for 4/6 ,5/6 ,6/6 HLA After a formal search is begun, takes an average of 4 months to transplant, if a donor is available. When a match is found, can take only a few days for confirmatory and special testing and shipment to the Transplant Center (less than 24 hours in an emergency).

6. Cryo-save India (part of Cryo-Save NV) Collection and Processing of cord blood and cord lining stem cells

8. Cryo-save India (part of Cryo-Save NV) Collection process Cord Lining Tube Cord Blood Bag Maternal Blood Sample Tube Kit Box

10. Cryo-save India (part of Cryo-Save NV) Therapy using stem cells and Cord Blood Stem Cells

12. How is Therapy done using stem cells

13. Real people, real stories..! The most precious gift! Proven therapy 06/16/09 Cryo-save India (part of Cryo-Save NV)

14. 06/16/09 Cryo-save India (part of Cryo-Save NV) What do these people have in common ? Nicholas Henderson – T cell Lymphoma Austin – Myelo Dysplastic Syndrome Tom Stretch – Chronic Granulomatous Disease Hemant Bebera - Thalassemia Blayke & Garrett LaRue – X Linked Lympho-proliferative Disorder Erica Nader – Spinal cord injury Hank Heacock - Leukemia All these patients were treated with Stem Cells at various centres.

15. Cryo-save India (part of Cryo-Save NV) Therapy in India AIIMS team shows how stem cells save hearts. Taken from bone marrow, they can revitalize non-functional heart muscles.

16. 06/16/09 Cryo-save India (part of Cryo-Save NV)

19. 06/16/09 Cryo-save India (part of Cryo-Save NV)

20. January 10, 2009 The Times of India.

22. Most recent applications (India) 1.Diabetes (National Centre for Cell Sciences, Pune University – Dr. Ramesh Bhonde) 2.Heart (Dr. Venugopal Rao – AIMS) 3.Liver Disease 4.Parkinson's Disease (Manipal Dr. Satish Totey) 5.Spinal Cord Injury (AFMC, Pune) 6.Stroke 7.Alzheimer's Disease

23. Cryo-save India (part of Cryo-Save NV) Company Profile

24. Europe’s Leading Stem Cell Bank Now in India

27. Cryo-Save India is situated in Whitefield, Bangalore and this fully automated laboratory which conforms to GLP is placed in an area of 10,000Sqft, this laboratory can store initially 1,50,000 samples which will make it the largest such storage facility in Asia. Cryo-Save India

31. Enrollment and Logistics

32. Registration Process Registration Kit Box Delivery Collection Dispatch Intimation to Doctor Paramedic Doctor

33. Post Registration procedure Sample Received at Lab Sample Processing Maternal Sample Inspection Cryo preservation Final Report to Client

34. Cryo-save India (part of Cryo-Save NV) Payments and refund options 3 PAYMENT OPTIONS Sr No. Plan Ref Plan options First Payment Second Payment Third Payment 1 CS08001 Standard option 1 75,000 2 CS08002 Standard option 2 25,000 20,000 30,000 3 CS08003 Standard Option 3 25,000 11 PDC’s of Rs 5000

36. All the best