2. Proportions of FMD Virus Serotypes Isolated by the WRLFMD O A C SAT1 SAT2 SAT3 ASIA1 (n=311) 64% 7% 23% 4% 2%

13. OIE Recommendation (2006) Schematic representation of laboratory tests for determining evidence of FMDV infection through or following serological surveys

15. Differentiation Infected and Vaccinated Animal Results serology Conventional test(LPB,VN) FMDV NSP test Vaccinated Infected + + Vaccinated Not infected + - Not vaccinated Infected + + Not vaccinated Not infected - -

16. Immune response of infected and vaccinated animals (cattle) +ve -ve Result Days after vaccination or infection 4 8 12 16 SP antibodies ( Infected) SP antibodies (Vaccinated) NSP antibodies ( Infected ) NSP antibodies (Vaccinated)

18. Test procedure NEGATIVE Only control band visible POSITIVE Control and test bands visible INVALID No control line, test is invalid Collect Blood / whole blood assays can be formatted Separate serum Take out the test card from the aluminium foil pouch and place it on a horizontal surface. Add 3 drops of the specimen into sample hole ‘S’ Take 3 drops serum

21. Program flow chart for Screening Dairy Animals

22. Program Flow Chart for Screening Slaughter Animals The practical screening scenario 40 animals/Truck With 3 staff (veterinary doctor and/or livestock inspectors) the total time for one vehicle: 57 minutes. In addition to inspection, the check-post authority will also note animal identification numbers on the issued certificate and on individual animals. ubio can provide custom kits with paired random number identification stickers for this purpose

23. Vigilance program