2. 蛋白组芯片 Proteome Profiler 产品 & 目录价格 Feature 5940 Proteome Profiler Rat Cytokine Array Kit, Panel A ARY008 5940 Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006 5940 Proteome Profiler Human Cytokine Array Kit, Panel A ARY005 5940 Proteome Profiler Human Phospho-Immunoreceptor Array Kit ARY004 5940 Proteome Profiler Human Phospho-MAPK Array Kit ARY002 5940 Proteome Profiler Human Phospho-RTK Array Kit ARY001 New Proteome Profiler Human Angiogenesis Array Kit ARY007 New 5940 Proteome Profiler Human Apoptosis Array Kit ARY009 New 8340 Proteome Profiler Human Phospho-Kinase Array Kit ARY003 RMB Description Cat#

7. 实验流程 Feature Block Arrays Mix Sample/ Detection Abs 1 hour O/N Incubate with Sample/Detection Abs Wash Wash SA-HRP 1/2 hour 1/2 hour 1/2 hour Chemiluminescent Detection ARY007 ~ 3 hrs 化学发光检测 Chemiluminescent Detection 孵育 Incubate with Sample 洗脱 Wash 洗脱 Wash 捕获抗体 Detection Abs 1 or 2 hours 封闭 Block Arrays 洗脱 Wash SA-HRP ARY003 & 9 ~4.5/ 5.5 hrs

9. ARY009 - 研究细胞凋亡 - 高通量 Advantage

10. 蛋白组芯片 Proteome Profiler 终端客户收获 Benefit 5.581 J Biol Chem ARY002 3.695 Exp. Cell Res. ARY002 4.034 Infect Immun ARY005 4.627 J Leukoc Biol. ARY005 4.887 Cell Signal ARY005 9.643 Proc. Natl. Acad. Sci. U.S.A. ARY005 10.896 Blood ARY005 4.159 Biochem J ARY001 5.581 J Biol Chem ARY001 7.672 Cancer Res. ARY001 10.896 Blood ARY001 15.484 J CLIN ONCOL ARY001 26.372 Science ARY001 SCI 影响因子 发表期刊 货号

11. 使用 Proteome Profiler 的客户 后续还会使用？ 关联销售 ARY007 人 - 血管生成芯片 Human Angiogenesis Array Benefit

13. 血管生成 分型 - 肿瘤组织裂解液中的相关蛋白 C 前列腺癌 Prostate Cancer 卵巢癌 Ovarian Cancer 乳腺癌 Breast Cancer

14. 缺氧对可溶性因子的影响 EFFECT OF HYPOXIA ON SOLUBLE FACTORS 未处理 1 2 4 3 5 6 7 8 CoCl2 DFO 去铁胺 GM-CSF 8 GDNF 7 Serpin F1/PEDF 6 MMP-9 5 HB-EGF 4 DPPIV/CD26 3 Serpin E1/PAI-1 2 CXCL16 1 1 2 4 3 5 6 7 8

16. 使用 Proteome Profiler 的客户 后续还会使用？ ARY007 人 - 血管生成芯片 Human Angiogenesis Array

18. Step 2: 细胞溶解 Cell Lysates Prepare 2 mL of diluted cell lysate. Remove Array Buffer 1. Add 1 mL of lysate to Part A and 1 mL of lysate to Part B. Incubate overnight at 4 °C on a rocking shaker. Step 3: 洗脱 Wash 1 Wash in 1X Wash Buffer, 20 mL per dish Wash 3 times, 10 minutes per wash. Wash corresponding parts (A and B) together. ARY003 流程 Step 1: 封闭 Blocking Add 1 mL Array Buffer 1 per well Rock for 1 hour at room temperature A001 A002 A003 A004 B001 B002 B003 B004 A001 A002 A003 A004 B001 B002 B003 B004 Lysate 1 Lysate 2 Lysate 3 Lysate 4 A001 B001 A002 A003 A004 B002 B003 B004

19. Transfer arrays to appropriate wells. Incubate 2 hours at room temperature on a rocking shaker. DC A DC B Step 4: 检测抗体混合液 Detection Antibody Cocktail Pipet 1 mL of diluted Detection Cocktail A (DC A) into wells for Part A arrays. Pipet 1 mL of diluted Detection Cocktail B (DC B) into wells for Part B arrays. Step 5: 洗脱 Wash 2 Wash in 1X Wash Buffer, 20 mL per dish. Wash 3 times, 10 minutes per wash. Wash all arrays separately. A001 A002 A003 A004 B001 B002 B003 B004 A001 A002 A003 A004 B001 B002 B003 B004

20. Step 6: Streptavidin-HRP Pipet 1 mL of diluted Streptavidin-HRP into each well. Transfer arrays to appropriate wells. Incubate 30 min. at RT on rocking shaker. Step 7: 洗脱 Wash 3 Wash in 1X Wash Buffer, 20 mL per dish. Wash 3 times, 10 minutes per wash. Wash corresponding parts (A and B) together. Step 8: 信号检测 Signal Detection Arrange arrays on sheet protector. Apply ECL reagent. Expose to film. A001 A002 A003 A004 B001 B002 B003 B004 A001 A002 A003 A004 B001 B002 B003 B004 A001 B001 A002 A003 A004 B002 B003 B004 MCF-7 Cells Untreated vs. UV Treated 1 2 3 4 5 6 7 8 Untreated UV Treated

22. ARY009- 细胞凋亡 Apoptosis 客户 : 学术院校，政府实验室，工业客户 研究领域： - 基础细胞凋亡研究 - 肿瘤研究 / 化学疗法研发 * 细胞凋亡不充分会导致无法控制的细胞增殖 New ARY009

23. ARY009 - 细胞凋亡研究工具

24. CoCl 2 处理 MCF-7 未处理 处理后

25. 人细胞凋亡蛋白芯片可用于 筛选 抑制剂 未处理 Camptothecin 喜树碱 Camptothecin + Caffeine 喜树碱 + 咖啡因 p53-S15 p53-S46 p53-S392