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Updates from Ohio State and  Camelid Parasite Diagnosis, Control, and Prevention Rebecca Pentecost, DVM Hospital for Farm Animals Veterinary Clinical Sciences
Objectives of Lecture –  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Update from Ohio State! ,[object Object],[object Object],[object Object]
Update from Ohio State Alpaca embryo transfer program ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],Update from Ohio State Alpaca embryo transfer program
Alpaca embryo –  Inner cell mass
Pharmacokinetics and bioavailability of florfenicol in Alpacas
Introduction ,[object Object],[object Object],[object Object]
Study design ,[object Object],[object Object],[object Object],[object Object],[object Object]
Study day preparation - IV ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Study day preparation – IM,SQ ,[object Object]
Sample Analysis ,[object Object],[object Object],[object Object],[object Object],[object Object]
Method characteristics ,[object Object],[object Object],[object Object],[object Object]
Pharmacokinetic analysis ,[object Object],[object Object],[object Object],[object Object]
Parameter Units Median Range A 1  (A) μg/mL 110 52  –  275 λ 1  ( α ) hr -1 4.48 2.57  –  14.8 A 2  (B) μg/mL 7.6 4.7  –  31 λ 2  ( β ) hr -1 0.367 0.099  –  1.04 k 31 hr -1 1.44 0.33  –  4.66 k 1e hr -1 1.32 0.54  –  2.0 k 13 hr -1 2.0 0.89  –  9.77 MRT hr 2.56 0.86  –  7.36 T ½  (elim) Hr 3.75 1.97  –  6.95 V ss L/kg 0.525 0.25  –  2.54 CL mL/min/kg 5.6 1.6  –  6.2 AUC μg*hr/mL 59.8 54  –  208 T max hr 0.00016 0.00011  –  0.014 C max μg/mL 109 52 - 275
Serum concentration-time curve  IV – Florfenicol (20 mg/kg)
Concentration-time curves  IM and SQ – Florfenicol (20 mg/kg)
Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object]
Mycoplasma haemolamae Transmission, virulence, and in vitro culture Morris Animal Foundation grant funded
Introduction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Acridine orange staining ,[object Object]
Clinical and Subclinical Disease ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transmission ,[object Object],[object Object],[object Object],[object Object]
Study Goals ,[object Object],[object Object],[object Object]
Study Design ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Sample Processing ,[object Object],[object Object],[object Object],[object Object]
Colostral Antibodies ,[object Object],[object Object],[object Object],[object Object]
Culture ,[object Object]
Initial Results and Conclusions ,[object Object],[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object]
Stifle Arthroscopy in Camelids
Study Goal and Design ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Joint Model ,[object Object],[object Object],[object Object]
Stife Arthroscopy
Selected Cases ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object]
Pharmacokinetics of Midazolam in South American Camelids Anesthesia and Farm Animal Sections Collaborative Project Dr. Pam Fry, Dr. Turi Aarnes,  Dr. John Hubbell, Dr. Jeff Lakritz
Introduction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Study Design ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Results Pending ,[object Object],[object Object],[object Object],[object Object],[object Object]
Future Camelid Research at Ohio State ,[object Object],[object Object],[object Object],[object Object],[object Object]
Update from Ohio State! ,[object Object],[object Object],[object Object],[object Object]
Buckeye Alpaca Show sponsors ICHC speakers ,[object Object],[object Object]
ICHC for DVM meeting – tentative schedule ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
ICI update –  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Parasites and Camelids –  Should We Be Worried? Becky Pentecost, DVM The Ohio State University Veterinary Medical Center Hospital for Farm Animals
The top 10 reasons camelids develop GI disorders –  ,[object Object],[object Object],[object Object],[object Object]
Why discuss parasites?  ,[object Object],[object Object],[object Object],[object Object],[object Object]
Parasitism in SAC ,[object Object],[object Object],[object Object],[object Object],[object Object]
Common Nematode Parasites GI Tract Location Parasites C3 Haemonchus contortus, Trichostrongylus, Ostertagia, Camelostrongylus, Teladorsagia, and Marshallagia Small Intestines Nematodirus, Cooperia, Trichostrongylus Large Intestines/Cecum Trichuris, Capillaria, and Oesophagostumum spp
Life Cycle Review for Trichostrongyle-types Infective larvae are ingested during grazing Eggs hatch, larvae develop to L3 in soil and manure Eggs passed onto pasture in manure Adult nematodes in the digestive tract of camelids lay their eggs.
Cestode Parasites ,[object Object],[object Object],[object Object],[object Object]
Protozoal Parasites ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Coccidia ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
E. mac – A Big Deal? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Indications of GI Parasitism ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Fecal Exams ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Other Diagnostics? ,[object Object],[object Object],[object Object]
Anthelmintics ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Available Dewormers Class Drug Dosage Route Avermectins Ivermectin 1.5 mL/100 lbs SQ Doramectin 2.0 mL/100 lbs SQ Cydectin Benzamidazoles Fenbendazole 9 mg/lb Oral Albendazole 5.5 mg/lb Oral Imidazothiazole derivative Levamisole 4 mg/lb Oral Depolarizing neuromuscular blocking agent Pyrantel pamoate 8 mg/lb Oral Antiprotazoal/ Coccidiostats Ponazuril 9 mg/lb Oral Toltrazuril Amprolium Sulfadimethoxine
Deworming Conundrum ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Monthly deworming with Ivermectins – How effective against whipworms?   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Monthly deworming with Ivermectins – How effective against mites? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
FECR tests – Goats 2001 Terrill TH, et al., 2001; Vet. Parasitol. 97:261-268 Anthelmintic EPG (2 wk post) FECR % (95% CI) Control 3827 ABZ 1450 62 (35-78) Dora 917 76 (39-91) FBZ 3460 10 (0-42) IVM 844 78 (5-95) LEV 335 91 (81-96) Morantel 1990 48 (11-70) MOXI 0 100 (100) ABZ + IVM 445 88 (74-95)
FECR tests – Goats 2003 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Mortenson LL, et al., 2003; JAVMA 223:495-500
FECR tests – Sheep and Goats ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Howell SB, et al., 2008; JAVMA 233:1913-1919
Parasite resistance in SAC? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Parasite resistance and anemia ,[object Object],[object Object],[object Object]
H. Contortus – C3, alpaca
Blood loss ? ,[object Object],[object Object],[object Object],[object Object],[object Object],Moore DM, Vet. Hematology; 5 th  Ed. Pp. 1184-90 Le Jambre LF, Int. J. Parasitol, 1995; 25:269-73 Rowe JB, et al., British J Nutrition 1988; 59:125-39
Fecal ooccult blood –
The Predicament – Death of your animals ,[object Object],[object Object],[object Object],[object Object]
Building a parasite program ,[object Object],[object Object],[object Object],[object Object]
Peculiarities of camelids –  ,[object Object],[object Object],[object Object]
Map of property – Why?
Integrated parasite management ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Grazing strategy ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Other Management Techniques ,[object Object],[object Object],[object Object],[object Object]
 
Anemia in South American camelids –  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Review of recent anemia cases from OSU VMC ,[object Object],[object Object],[object Object]
Iron deficiency anemia –  ,[object Object],[object Object],[object Object]
Iron deficiency anemia ,[object Object],[object Object],[object Object],[object Object],[object Object],From: Smith BP, 2009; 4 th  Ed., 2009
Total plasma protein and red cell parameters – presentation Analyte Mean  ± sd Range Median Reference TPP (Gm/dL) 5.2  ± 0.72 3.7 – 6.6  5.3 5.4 – 7.2 HCT (%) 9.9 ± 5.3 4 – 25 8 24 – 35 HgB (Gm/dL) 4.2 ± 2.3 1.6 – 11 3.6 9.1 – 16.2 RBC (x 10 12 /L) 4.2 ± 2.5 1.4 – 10.5 3 8.8 – 15.4 MCV (fL) 25 ± 6 12.7 - 44 24 21 - 30 MCH (pg) 10 ± 2 6.5 – 16 10 7.7 - 13 MCHC (g/dL) 42 ± 5.7 35.7 - 48 41 39 - 46 NRBC (ABS) 28  ± 47 0 - 257 15 0 – 0 NRBC (#/100 WBC) 4.8 ± 8.2 0 - 42 2 0 – 1
RBC abnormalities - ?
Polychromatic, hypochromatic, dacryocytes
Serum Fe, TIBC, UIBC in anemic Alpacas Data from 13 anemic and 13 normal Alpacas *Indicates significant difference from normal using t-test (P<0.05); power with  α  = 0.05 are 0.915, 0.67 respectively Analyte Units Anemic (median) Range Normal (median) Range Serum  Fe μ g/dL 43  ± 42 * (22) 2 – 121 95  ± 35 (96) 37 - 168 TIBC μ g/dL 261  ± 65 (266) 172 – 363  248  ± 44 (242) 186 – 339 UIBC μ g/dL 218  ± 71  * (209) 70 - 309 153  ± 51 (164) 61 – 230
“ Otis May” – Presentation  ,[object Object],[object Object],[object Object],[object Object]
Case Management ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Questions?

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Dr. Rebecca Pentecost: Pennsylvania Llama and Alpaca Association 2011

  • 1. Updates from Ohio State and Camelid Parasite Diagnosis, Control, and Prevention Rebecca Pentecost, DVM Hospital for Farm Animals Veterinary Clinical Sciences
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  • 6. Alpaca embryo – Inner cell mass
  • 7. Pharmacokinetics and bioavailability of florfenicol in Alpacas
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  • 15. Parameter Units Median Range A 1 (A) μg/mL 110 52 – 275 λ 1 ( α ) hr -1 4.48 2.57 – 14.8 A 2 (B) μg/mL 7.6 4.7 – 31 λ 2 ( β ) hr -1 0.367 0.099 – 1.04 k 31 hr -1 1.44 0.33 – 4.66 k 1e hr -1 1.32 0.54 – 2.0 k 13 hr -1 2.0 0.89 – 9.77 MRT hr 2.56 0.86 – 7.36 T ½ (elim) Hr 3.75 1.97 – 6.95 V ss L/kg 0.525 0.25 – 2.54 CL mL/min/kg 5.6 1.6 – 6.2 AUC μg*hr/mL 59.8 54 – 208 T max hr 0.00016 0.00011 – 0.014 C max μg/mL 109 52 - 275
  • 16. Serum concentration-time curve IV – Florfenicol (20 mg/kg)
  • 17. Concentration-time curves IM and SQ – Florfenicol (20 mg/kg)
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  • 20. Mycoplasma haemolamae Transmission, virulence, and in vitro culture Morris Animal Foundation grant funded
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  • 38. Pharmacokinetics of Midazolam in South American Camelids Anesthesia and Farm Animal Sections Collaborative Project Dr. Pam Fry, Dr. Turi Aarnes, Dr. John Hubbell, Dr. Jeff Lakritz
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  • 47. Parasites and Camelids – Should We Be Worried? Becky Pentecost, DVM The Ohio State University Veterinary Medical Center Hospital for Farm Animals
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  • 51. Common Nematode Parasites GI Tract Location Parasites C3 Haemonchus contortus, Trichostrongylus, Ostertagia, Camelostrongylus, Teladorsagia, and Marshallagia Small Intestines Nematodirus, Cooperia, Trichostrongylus Large Intestines/Cecum Trichuris, Capillaria, and Oesophagostumum spp
  • 52. Life Cycle Review for Trichostrongyle-types Infective larvae are ingested during grazing Eggs hatch, larvae develop to L3 in soil and manure Eggs passed onto pasture in manure Adult nematodes in the digestive tract of camelids lay their eggs.
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  • 61. Available Dewormers Class Drug Dosage Route Avermectins Ivermectin 1.5 mL/100 lbs SQ Doramectin 2.0 mL/100 lbs SQ Cydectin Benzamidazoles Fenbendazole 9 mg/lb Oral Albendazole 5.5 mg/lb Oral Imidazothiazole derivative Levamisole 4 mg/lb Oral Depolarizing neuromuscular blocking agent Pyrantel pamoate 8 mg/lb Oral Antiprotazoal/ Coccidiostats Ponazuril 9 mg/lb Oral Toltrazuril Amprolium Sulfadimethoxine
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  • 65. FECR tests – Goats 2001 Terrill TH, et al., 2001; Vet. Parasitol. 97:261-268 Anthelmintic EPG (2 wk post) FECR % (95% CI) Control 3827 ABZ 1450 62 (35-78) Dora 917 76 (39-91) FBZ 3460 10 (0-42) IVM 844 78 (5-95) LEV 335 91 (81-96) Morantel 1990 48 (11-70) MOXI 0 100 (100) ABZ + IVM 445 88 (74-95)
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  • 70. H. Contortus – C3, alpaca
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  • 76. Map of property – Why?
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  • 85. Total plasma protein and red cell parameters – presentation Analyte Mean ± sd Range Median Reference TPP (Gm/dL) 5.2 ± 0.72 3.7 – 6.6 5.3 5.4 – 7.2 HCT (%) 9.9 ± 5.3 4 – 25 8 24 – 35 HgB (Gm/dL) 4.2 ± 2.3 1.6 – 11 3.6 9.1 – 16.2 RBC (x 10 12 /L) 4.2 ± 2.5 1.4 – 10.5 3 8.8 – 15.4 MCV (fL) 25 ± 6 12.7 - 44 24 21 - 30 MCH (pg) 10 ± 2 6.5 – 16 10 7.7 - 13 MCHC (g/dL) 42 ± 5.7 35.7 - 48 41 39 - 46 NRBC (ABS) 28 ± 47 0 - 257 15 0 – 0 NRBC (#/100 WBC) 4.8 ± 8.2 0 - 42 2 0 – 1
  • 88. Serum Fe, TIBC, UIBC in anemic Alpacas Data from 13 anemic and 13 normal Alpacas *Indicates significant difference from normal using t-test (P<0.05); power with α = 0.05 are 0.915, 0.67 respectively Analyte Units Anemic (median) Range Normal (median) Range Serum Fe μ g/dL 43 ± 42 * (22) 2 – 121 95 ± 35 (96) 37 - 168 TIBC μ g/dL 261 ± 65 (266) 172 – 363 248 ± 44 (242) 186 – 339 UIBC μ g/dL 218 ± 71 * (209) 70 - 309 153 ± 51 (164) 61 – 230
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Notas del editor

  1. Eggs produced by females Passed in manure Larva develop in fecal mass L1 hatches, feeds on bacteria, undergoes two molts L3 makes way out of feces onto forage Ingested Develops to L4, to immature adult After maturation, starts producing eggs Prepatent period is appx 3 weeks Occasional hypobiotic periods (RARE) Weather dependent (shown in Great Britain study, cold then warming to 10 C with moist environment needed to hatch.
  2. Fecal egg count reduction test (FECRT) – eggs/gram at pretreatment levels versus those 10-14 days later. For practical purposes, a 90% reduction suggests successful treatment and &lt;90% reduction may indicate resistant parasites, ineffective dose or route of administration. Egg count concerns – &lt;200 epg are usually considered ok if BCS is also normal. If epg is rising, animals have poor bcs or other signs of ill thrift, it is possible treatment may be helpful. Epg significance is much lower when considering low or intermittently shedding parasites like trichuris, capillaria, nematodirus, or emac (even low numbers may warrant treatment).
  3. Not ideal to blanket treat entire herd or to rotate dewormers regularly as this may lead to the development of resistant parasites. The ideal approach involves selectively treating animals based on fecal counts and clinical signs (20% of animals harbor 80% of the herd parasite burden). Targeted deworming allows one to leave a population of parasites that have not been exposed to specific drugs. Leaving these naïve parasites helps prevent selection for resistant parasites (refugia, as coined by parasitologists).
  4. Large volume of blood lost by animal. 10 days of losing 200mL = 2L