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Goal       Usage         Implementation   Availability




       Next-generation sequencing
       request management system
                in Galaxy

                   Brad Chapman


                   16 May 2010
Goal          Usage        Implementation       Availability




       Researchers                  Sequencing Core
                      Samples




                      Analysis
Goal    Usage     Implementation   Availability



Sample and request infrastructure
Goal       Usage       Implementation   Availability



Functionality


       Researcher sample entry
       Sequencing tracking and management
       Layout of flow cells
       Full integration with Galaxy
Goal     Usage      Implementation   Availability



Multiple services
Goal    Usage   Implementation   Availability



Sample entry
Goal    Usage     Implementation   Availability



Organize as projects
Goal    Usage    Implementation   Availability



Project based view
Goal   Usage    Implementation   Availability



Tracking and management
Goal     Usage     Implementation   Availability



Flow cell layout
Goal             Usage           Implementation    Availability



Leverages Galaxy infrastructure

  Display                        Database
       grids                         samples
       form builder                  requests
                                     events
                                     form values
       Added to database
        One relationship table
Goal                       Usage                           Implementation                         Availability



Sample request relationships

                                                 Next-gen              Next-gen
       Requests:                                 Sequencing            Sequencing
                                                 Run 1                 Run 2




                                  Sample 2                    Sample                 Sample 3




                   Library                       Pre-sequencing                 Sequencing   Sequencing
       Requests:   Construction
                                    Validation
                                                 quantitation
                                                                  Sequencing
                                                                  Details       results 1    results 2
Goal         Usage         Implementation   Availability



Community


  Positives                Suggestions
       Consistent              High level update
       interface               summaries
       Excellent backend       Internals
       Maintainability         documentation
                               Roadmap
Goal            Usage        Implementation     Availability




       Availability
       Repository tracking Galaxy development

       http://bitbucket.org/chapmanb/
       galaxy-central

       Hope to integrate with the main code base

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Next-generation sequencing request management system in Galaxy

  • 1. Goal Usage Implementation Availability Next-generation sequencing request management system in Galaxy Brad Chapman 16 May 2010
  • 2. Goal Usage Implementation Availability Researchers Sequencing Core Samples Analysis
  • 3. Goal Usage Implementation Availability Sample and request infrastructure
  • 4. Goal Usage Implementation Availability Functionality Researcher sample entry Sequencing tracking and management Layout of flow cells Full integration with Galaxy
  • 5. Goal Usage Implementation Availability Multiple services
  • 6. Goal Usage Implementation Availability Sample entry
  • 7. Goal Usage Implementation Availability Organize as projects
  • 8. Goal Usage Implementation Availability Project based view
  • 9. Goal Usage Implementation Availability Tracking and management
  • 10. Goal Usage Implementation Availability Flow cell layout
  • 11. Goal Usage Implementation Availability Leverages Galaxy infrastructure Display Database grids samples form builder requests events form values Added to database One relationship table
  • 12. Goal Usage Implementation Availability Sample request relationships Next-gen Next-gen Requests: Sequencing Sequencing Run 1 Run 2 Sample 2 Sample Sample 3 Library Pre-sequencing Sequencing Sequencing Requests: Construction Validation quantitation Sequencing Details results 1 results 2
  • 13. Goal Usage Implementation Availability Community Positives Suggestions Consistent High level update interface summaries Excellent backend Internals Maintainability documentation Roadmap
  • 14. Goal Usage Implementation Availability Availability Repository tracking Galaxy development http://bitbucket.org/chapmanb/ galaxy-central Hope to integrate with the main code base