The Anoxia technique is developed as alternative for existing animal stunning methods that are based on the use of CO2, electrocution, neck dislocation, captive-bolt, as well as killing methods like de-bleeding and maceration. In the past 10 years, Wageningen University and University of Glasgow conducted several studies that proved that the technique could be applied successfully for culling poultry (Proof of Principle Anoxia Technique). This was the start of the development of several applications based on the Anoxia principle, using high expansion foam filled with >99% Nitrogen that are now introduced for: 1. Stunning and killing of sick and cripple killing piglets less than 5 kg 2. Stunning and killing of sick or cripple poultry (especially poultry > 3kg) who need to be killed on the farm by the staff for welfare purposes (avoiding unnecessary stress or pain) 3. Stunning and killing poultry that arrives on the slaughterhouse but that are unfit to be slaughtered (due to injuries occurred during transportation – providing signs of possible illness etc.) 4. Stunning and killing of male pullets at the hatchery 5. Stunning and killing of half-hatched chickens and embryos in partly-hatched eggs, before destruction 6. Stunning and killing parent stock poultry 7. Killing of animals that has been stunned (captive bolt – blow-on-the-head method, etc.) replacing killing by de-bleeding 8. Culling of ex-layers 9. Culling of poultry for disease control purposes Last November we started the launch of the commercialization of the Anoxia applications in Holland, Germany and Sweden, focusing on the areas where a solution is most needed: piglets (< 5kg) and poultry (> 3kg) on farms. Since November 2016, the introduction of these applications took place in Holland, Germany, Sweden and Denmark

1. www.anoxia.eu
Presentation 1
The Anoxia technique:
A worthy end for each animal
For more information: www.anoxia.eu

2. Anoxia What is it?
Presented by the N2GF team
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3. Anoxia: a natural phenomenon
– Oxygen must be present in every breathing gas
because it is essential to the body's metabolic
process
– As the altitude increases (from > 2,400 meter)
atmospheric pressure decreases, oxygen levels drop
– At 30 meters below sea level atmospheric pressure
is 4 times the normal atmosphere, leading to higher
CO2 and lower Oxygen levels.
– A decrease in blood oxygen level leads to relative
increase of the nitrogen concentration inhaled,
resulting in Hypoxic Anoxia
– In the medical literature, the effects of exposure to
high concentrations of nitrogen is described as
Nitrogen Asphyxiation
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4. • In a plane you are instructed what to do in
case the cabin pressure drops ..
• When climbing Mount Everest, you need
additional oxygen, unless you are a Sherpa ..
• In case you are diving, you are instructed
what to do when you dive below 30 meters
..
Anoxia: Never heard about it?
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5. Hemoglobin: its crucial role in the transport
of gasses through the body
• Hemoglobin is the iron-
containing oxygen-transport
metalloprotein in the red blood
cells of all vertebrates
• Hemoglobin enables the
transport of gasses throughout
the body
• It carries oxygen from the
respiratory organs (lungs) to the
rest of the body where it
releases the oxygen
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6. • No physical responses; the air we breath
consists of 78% Nitrogen, so high quantities
of remain undetected ..
• No stress or pain sensations, on contrary;
mountain climbers and divers describe
Anoxia as reaching a state of positive anxiety
..
• Minimum reaction by animals when they
are exposed to Anoxia until the moment
that they turn unconscious ..
Anoxia caused by exposure to >98% Nitrogen:
What’s happening?
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7. The technical breakthrough:
Fill large low expansion foam bubbles with 100% Nitrogen
• Invented in Holland in 2003, as alternative method for existing
CO2 techniques used for mass killing animals during outbreaks
• Since 2006 successfully tested as stunning method of poultry
by scientists in Germany, Holland, and The UK
• The anoxia technique contributes to todays economic, social,
environmental, public health and consumer welfare objectives
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For more information: www.anoxia.eu

8. The Anoxia technique:
A worthy end for each animal
For more information: www.anoxia.eu