This presentation was originally intended for the NHOS conference. Now optimized for website use. Owned by www.crossroadsesp.org

1. Saving the Beautiful Lady’s Slippers
Crossroads Academy Endangered Species Program
http://www.crossroadsesp.org
~ Special thanks to Elaine Faletra and Fernando & Noah Ramos for their help with photography ~

2. The Pink Lady’s Slipper Cypripedium acaule

3. The Showy Lady’s Slipper Cypripedium reginae

8. Ovary
Pouch
Staminode
Stigma
Pollen

10. The Yellow Lady’s Slipper Cypripedium parviflorum

11. Staminode Pollen mass
Stigma
Mosquito

12. Ram’s Head Lady’s Slipper Cypripedium arietinum

14. Pollen mass
Stigma
Staminode

15. Pollen
Stigma
Staminode
The Pink Lady’s Slipper Cypripedium acaule

17. How did this all start?

18. The Program at Crossroads
 Study how to grow them in
sterile culture
 How to transplant them to
soil
 how to get them to survive
in the wild
 create sanctuaries around the
state

19. Our Lab

20. What are we culturing?
We are culturing
4 slippers:
Cyp. reginae
Cyp. parviflorum
Cyp. arietinum
Cyp. acaule

21. What are we culturing?
We are culturing
4 slippers:
Cyp. reginae
Cyp. parviflorum
Cyp. arietinum
Cyp. acaule

22. What are we culturing?
We are culturing
4 slippers:
Cyp. reginae
Cyp. parviflorum
Cyp. arietinum
Cyp. acaule

23. What are we culturing?
We are culturing
4 slippers:
Cyp. reginae
Cyp. parviflorum
Cyp. arietinum
Cyp. acaule

24. How?
Seed
Flowering
Plant
Plant in Soil Seedling Vernalization

25. Seed

26. Cross-Pollination

27. First we need the seeds

28. Harvesting the Precious Seeds . . .

29. Slipper Seeds

30. The Seed of C. reginae

31. We Stripped Off the Outer Seed Coat

32. A seed sliced and stained

34. We use the
seeds to grow
seedlings in
sterile culture

35. Weakening the seed coat

36. Inoculating the Seeds

37. Inoculating the Seeds

38. Inoculating the Seeds

39. Inoculating the Seeds

40. Inoculating the Seeds

41. Sealing the Tubes

44. Stage 1 Stage 2
Stage 3
Stage 4

46. Seedlings
Shoots
Roots
Rhizomes

47. A drawing of a seedling
Two Entwined Seedlings
rhizomes
roots
shoots

48. Seedlings can be raised in baby food jars with a special
top called a magenta cap.
Economizing on Culture Containers

49. Scanning Electron micrograph

50. Showy slipper seedlings are available at our booth

51. Getting ready for vernalization. . .

55. Dirt

57. Soil-less

58. In Agar

61. Creating a Fen

66. Locating a home-made fen
 damp area
 ~ 6 hrs direct sunlight
 sweet soil pH ~ 7
 ground cover of
sphagnum moss and
ferns (nice to have)

70. Seedlings growing in our sanctuary

72. Making a Fen ...

83. Lady’s Slippers in the wild

84. Yellow Lady’s slippers in
a hardwood forest
Cyp. parviflorum
Lady’s Slippers in the wild

87. The Showy Lady’s
slipper
Cyp. reginae
Showy Lady’s Slippers in the wild

89. The Ram’s Head
Lady’s slipper
Cyp. arietinum
Ram’s Head Slipper in the wild

91. About ten ram’s head lady’s slippers past bloom
on June 4, 2011

92. The “stemless” or
Pink Lady’s slipper
Cyp. acaule
Pink Lady’s Slippers in the wild

94. A fen on our campus showing
some of the typical understory plants

95. If you have any questions regarding
this presentation. Please use our
forum or contact module to ask your
questions.
Presentation originally used for the NHOS orchid lovers conference.
Optimized for Web Use. (Version 1.2)