10. Micropropagatio
n
Advantages
• From one to many propagules rapidlyFrom one to many propagules rapidly
• Multiplication in controlled lab conditionsMultiplication in controlled lab conditions
• Continuous propagation year roundContinuous propagation year round
• Potential for disease-free propagulesPotential for disease-free propagules
11. Conventional
Propagation
Advantages
• Equipment costs minimalEquipment costs minimal
• Experience can substitute for technical expertiseExperience can substitute for technical expertise
• Costs per unit are minimalCosts per unit are minimal
• Potential for disease-free propagulesPotential for disease-free propagules
• Specialized techniques for growth control (graftingSpecialized techniques for growth control (grafting
onto dwarfing rootstocks)onto dwarfing rootstocks)
12. Micropropagatio
n
Limitations
• Equipment/facility intensive operationEquipment/facility intensive operation
• Technical expertise in management positionsTechnical expertise in management positions
• Protocols not optimized for all speciesProtocols not optimized for all species
• Liners may not fit industry standardLiners may not fit industry standard
• Propagules may be too expensivePropagules may be too expensive
13. Micropropagatio
n
Applications
• Rapid build up of stock of new, superior varietyRapid build up of stock of new, superior variety
• Elimination of endophytic diseaseElimination of endophytic disease
• Better suited to morphology/growth characteristicsBetter suited to morphology/growth characteristics
(few offshoots/sprouts; date palms, ferns, nandinas)(few offshoots/sprouts; date palms, ferns, nandinas)
• Propagules have enhanced growth featuresPropagules have enhanced growth features
(multibranched character; Ficus, Syngonium)(multibranched character; Ficus, Syngonium)
18. Somatic Embryogenesis
Stimulation of callus or suspension cells to undergo a
developmental pathway that mimics the development of
the zygotic embryo.
33. Chimera
Plant composed of two genotypes growing adjacent
to one another in all (or part) of the plant body.
34. Inflorescence culture
The inflorescence of many plants is a modified branching
system. Vegetative meristems in the inflorescence may be
stimulated to proliferate in vitro.
bract
flower bud
peduncle
axi
l