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KRISHGEN is leading manufacturer of ELISA kits for measuring markers in rat serum or plasma. Citations are available for majority of the products and can be viewed on the website.
rat cytokines general flyer
rat cytokines general flyer
krishgen
Kupffer Cells Mediate Leptin-Induced Liver Fibrosis. GASTROENTEROLOGY 2009;137:713–723 JIANHUA WANG,* ISABELLE LECLERCQ,‡ JOANNE M. BRYMORA,* NING XU,* MEHDI RAMEZANI–MOGHADAM,* ROSLYN M. LONDON,* DAVID BRIGSTOCK,§ and JACOB GEORGE* *Storr Liver Unit, Westmead Millennium Institute, University of Sydney and Westmead Hospital, Westmead, Australia; ‡Laboratory of Gastroenterology, Faculty of Medicine, Université Catholique de Louvain, Brussels, Belgium; and §Center for Cell and Vascular Biology, Children’s Research Institute, Columbus, Ohio 瘦素(Leptin)是一由脂肪細胞(Adipocyte)所分泌之荷爾蒙，是調控體重及新陳代謝之重要因子。過去研究發現病態肥胖(Obese)、脂肪肝(Nonalcoholic steatohepatitis)及酒精性肝炎(Alcoholic liver disease)等病患之血液循環中，Leptin量有明顯增加。而近期研究報告指出leptin具有促進肝臟纖維化(Liver fibrosis)之能力，當中分子機理並未明確。 在肝纖維化過程中，肝臟星狀細胞(HSC)會被活化增生及促進胞外基質(ECM)產生，而鄰近之Kupffer細胞(KC)則已知可透過促發炎因子(Proinflammatory factor)和促纖維化因子(Profibrogenic factors)例如TGF-β1和ROS影響HSC表現。雖然HSC是肝纖維化過程中重要角色，前人研究卻發現leptin似對HSC無任何調控作用。故本篇作者針對Leptin是否透過間接作用於HSC鄰近之KC，刺激其產生促纖維化因子，以活化HSC。 為探討leptin直接或間接影響HSC之分子機理，本篇作者透過RT-PCR、Immunoblot等分子生物學方法，分別測定leptin刺激後HSC及KC中Collagen I、TIMP1等促纖維化因子基因及蛋白表現，發現leptin雖可促使HSC增生，但對其纖維化能力之影響甚微。而leptin可刺激KC中TGF-β1及CTGF/CCN2等肝纖維化中重要之cytokines表現。另發現Leptin-treated KC-conditioned培養液可刺激HSC增生及增加其中Collagen I、TIMP1等表現，得出了leptin是透過刺激KC來活化HSC之推論。作者亦於後續實驗中，透過磷酸化測定、EMSA等方法探討leptin訊號傳遞作用，發現leptin可活化KC中STAT3、ERK1/2、AKT等路徑，及下游因子AP-1、NF-κB，而此兩種蛋白具有增強TGF-β1及CTGF/CCN2基因表現之能力。
Kupffer cells mediate_leptin-induced_liver_fibrosis
Kupffer cells mediate_leptin-induced_liver_fibrosis
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KRISHGEN is leading manufacturer of ELISA kits for measuring markers in rat serum or plasma. Citations are available for majority of the products and can be viewed on the website.
rat cytokines general flyer
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krishgen
Kupffer Cells Mediate Leptin-Induced Liver Fibrosis. GASTROENTEROLOGY 2009;137:713–723 JIANHUA WANG,* ISABELLE LECLERCQ,‡ JOANNE M. BRYMORA,* NING XU,* MEHDI RAMEZANI–MOGHADAM,* ROSLYN M. LONDON,* DAVID BRIGSTOCK,§ and JACOB GEORGE* *Storr Liver Unit, Westmead Millennium Institute, University of Sydney and Westmead Hospital, Westmead, Australia; ‡Laboratory of Gastroenterology, Faculty of Medicine, Université Catholique de Louvain, Brussels, Belgium; and §Center for Cell and Vascular Biology, Children’s Research Institute, Columbus, Ohio 瘦素(Leptin)是一由脂肪細胞(Adipocyte)所分泌之荷爾蒙，是調控體重及新陳代謝之重要因子。過去研究發現病態肥胖(Obese)、脂肪肝(Nonalcoholic steatohepatitis)及酒精性肝炎(Alcoholic liver disease)等病患之血液循環中，Leptin量有明顯增加。而近期研究報告指出leptin具有促進肝臟纖維化(Liver fibrosis)之能力，當中分子機理並未明確。 在肝纖維化過程中，肝臟星狀細胞(HSC)會被活化增生及促進胞外基質(ECM)產生，而鄰近之Kupffer細胞(KC)則已知可透過促發炎因子(Proinflammatory factor)和促纖維化因子(Profibrogenic factors)例如TGF-β1和ROS影響HSC表現。雖然HSC是肝纖維化過程中重要角色，前人研究卻發現leptin似對HSC無任何調控作用。故本篇作者針對Leptin是否透過間接作用於HSC鄰近之KC，刺激其產生促纖維化因子，以活化HSC。 為探討leptin直接或間接影響HSC之分子機理，本篇作者透過RT-PCR、Immunoblot等分子生物學方法，分別測定leptin刺激後HSC及KC中Collagen I、TIMP1等促纖維化因子基因及蛋白表現，發現leptin雖可促使HSC增生，但對其纖維化能力之影響甚微。而leptin可刺激KC中TGF-β1及CTGF/CCN2等肝纖維化中重要之cytokines表現。另發現Leptin-treated KC-conditioned培養液可刺激HSC增生及增加其中Collagen I、TIMP1等表現，得出了leptin是透過刺激KC來活化HSC之推論。作者亦於後續實驗中，透過磷酸化測定、EMSA等方法探討leptin訊號傳遞作用，發現leptin可活化KC中STAT3、ERK1/2、AKT等路徑，及下游因子AP-1、NF-κB，而此兩種蛋白具有增強TGF-β1及CTGF/CCN2基因表現之能力。
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2.4 gel
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Wilson disease-associated protein
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![We (group 1) found that our protein was AT7B_Human. ,[object Object]](https://image.slidesharecdn.com/2-4gel-110505105036-phpapp01/85/2-4-gel-1-320.jpg)
