3. Steps in Research (Holy 11)
1. Collect review of literature/Situation Analysis
2. Identify and prioritize health problems
3. Decide aims & objectives
4. Planning Methodology
5. Execution
6. Compilation, Classification & Presentation of
data
7. Analysis
8. Test of Significance/Test of Hypothesis
9. Inferences
10. Report Writing
11. Dissemination of Report
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4. Process of Concluding
8 7 6
Reporting Inferences Analysis
Data Collection
5
Execution
Execution
Research Problem
Define
1
for Pretest
Collection
Data
Review of Literature Methodology
4
2 3
Planning
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5. STEP-1
DEFINITION
OF THE
RESEARCH PROBLEM
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6. RESEARCH PROBLEM ?
Research Problem refers to some difficulty
which a researcher experiences and
wants to obtain a solution for the same.
i.e. a question or issue to be examined.
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7. Process of Defining Problem
Analysis of the Situation
Identify & Prioritize Problems
Select & Define Problem
Statement of
Research Objectives
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8. CRITERIA OF SELECTION
The selection of one appropriate researchable
problem out of the identified problems requires
evaluation of certain criteria.
* Internal / Personal criteria – Researcher‟s side
* External Criteria – Problem side factors
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9. INTERNAL CRITERIA OF SELECTION
Researcher‟s Interest,
Researcher‟s Competence,
Researcher‟s own Resource:
Human Resource
Money
Material
Time
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10. EXTERNAL CRITERIA OF SELECTION
Researchability of the problem,
Importance and Urgency,
Novelty of the Problem,
Feasibility,
Facilities,
Social Relevance
Public health Importance
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11. DEFINE RESEARCH PROBLEM
(Title of the Research Topic)
Transforming the selected research problem into a
scientifically researchable statement.
Problem definition or Problem statement should be
clear, precise, self-explanatory and include:-
What
How
When
Where
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12. RESEARCH OBJECTIVES
(Objectives)
Research Objectives are the statement of the
questions that is to be investigated with the goal of
answering the overall research problem.
Research Objectives should be clear and achievable.
Generally, they are written as statements, using the
word “to”
(For example, „to discover …‟, „to determine …‟, „to
establish …‟, „to find out -----‟, „to assess -----‟etc. )
Objectives should infer in the end of the study
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13. Hypothetical Research Question
Problem:
PCR of Diabetes Mellitus is increasing very
fast during last five year
Mission:
Reduce the incidence of heart disease
Belief:
Meditation is good to reduce stress which
is an important precursor of DM
Hypothesis
H- Meditation decreases the risk of DM
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14. Association of Garlic consumption with
coronary Artery Diseases
Aim: To Study the association of Meditation with
Diabetes Mellitus in patients attending at Medical
OPD of SMS Hospital, Jaipur (Raj) India.
Objectives:
1. To assess and compare the proportion of DM
cases in individuals doing regular meditation and
not doing meditation.
2. To find out the risk ratio of DM in individuals not
doing meditation on doing regular meditation.
15. STEP-2
REVIEW
OF
LITERATURE
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17. What ?
REVIEW OF LITERATURE
Literature Review is the documentation
of a published and unpublished work
from secondary sources of data
in the areas of specific interest to the researcher.
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18. Why ? - PURPOSE OF REVIEW
Tofind out already investigated problems and
those that need further investigation.
To formulate researchable hypothesis.
To gain a background knowledge
To identify data sources
To learn how others structured their reports.
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19. Where ?
SOURCES OF LITERATURE
Books and Journals
Databases
Bibliographic Databases
Abstract Databases
Full-Text Databases
Govt. and NGO Records & Reports
Internet
On line journals: ww.articalbase.com …….
E. Databases – Popline, Medline …….
Research Dissertations / Thesis
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21. Methodology
Study Area : Location of study - Hospital, community etc.
Study Period: Start to end of Study (maximum period
available for study should be defined)
*Selection of Study Design
* Selection of Study Population
Pre-requisits of study: Study Tools, Terminologies,
Orientation trainings etc.
*will be taken separately
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22. Methodology……
• Study Tools for data collection: subjects, proforma,
examination, measurements, lab investigations
• Planning
Data collection, compilation, data entry
Data cleaning
Analysis plan:
• Confidentiality
• Ethical clearance: Consent from
Institutional Review Board
Observational units
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23. Study Design
A study design is a specific plan or protocol
for conducting the study,
which allows the investigator
to translate the conceptual hypothesis
into an operational one.
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24. Direction of Study
Backward Forward
Cross -sectional
Retrospective Prospective
3
4. Ambidirectional
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25. Decision Tree
Intervention Done
No Yes
Observational Study Experimental Study
Comparison Group Randomization
No Yes
No Yes
Descriptive Study Analytic Study
NRCT Study RCT Study
Direction of Study
E O E O
Cohort Study E = O Case-Control Study
Cross-Sectional Study
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26. Epidemiological Study Design
Observational Studies
Descriptive Studies
Analytic
Cross-Sectional
Case-Control
Cohort
Experimental / Interventional studies
As per Control: RCT/NRCT
As per Blinding: Single /Double Blind
As per Design: Simple/Cross-over
As per Area: Field/Clinical/Lab
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27. Descriptive Studies
• Case reports
• Case series
• Population studies
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28. Descriptive Studies: Uses
• Hypothesis generating
• Suggesting associations
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29. Descriptive Type of Observational Study
• Other Name Case-Series/Population
• Unit of Study Case/Individuals
• Study Question What is happening
• Direction Of Inquiry
• Study Design
desired information
about cases/individuals is
collected
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30. Case-Series …….
Advantages
• Easy to do
• Excellent at identifying unusual situation
• Good for generating hypotheses
Disadvantages
• Generally short-term
• Investigators self-select (bias!)
• no controls
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32. Cross-sectional Study
• Data collected at a single point in time
• Describes associations
• Prevalence
A “Snapshot”
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33. Cross-Sectional Study
• Other Name Prevalence Study
• Unit of Study Individual
• Study Question What is happening
• Direction of Inquiry
• Study Design Exposed
to Factor
Not
Exposed
Diseased to Factor
Population Exposed to
Factor
Non-
Disease Not
Exposed to
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34. Objectives of a Cross-Sectional Study
To find out association
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35. Cross-sectional Study
Sample of Population
Defined Population
Regular Not doing meditation
Meditation
Prevalence of Prevalence of
DM DM
Time Frame = Present
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36. Cross-sectional Study
E.G. Out of 1000 population if 100 were doing meditation regularly &
out of that only 2 were having DM. Remaining 900 were not doing
meditation at all, out of that 220 were having DM.
+ DM -
2 98
Meditation
+
- 220 680
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37. Cross-Sectional Study
• Strengths
– Quick
– Cheap
• Weaknesses
– Cannot establish cause-effect
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38. Case-Control Studies
Start with people who have disease(Cases)
Match them with controls that do not have
disease (Match Confounding)
Look back and assess exposures
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39. Controls
A control is a standard of comparison
(confounded with variability but without effect)
for
• Effects
• Variability
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40. Case-Control Study
• Other Name Retrospective Study
• Unit of Study Cases/Control
• Study Question What has happened
• Direction of Inquiry= F O
• Study Design
Exposed
Cases
Not
Exposed
Exposed
Control
Not
Exposed
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41. Objective of a Case-Control Study
To find out association
To assess Risk Ratio
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42. Case-Control Study
Cases
Regular Meditation
Patients with DM
No Meditation
Controls
Regular Meditation
Persons w/o DM
No Meditation
Past Present
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43. The logic of Case-Control Studies
Cases differ from controls only in having the
disease
If exposure does not predispose to having
the disease, then exposure should be equally
distributed between the cases and controls.
The extent of greater previous exposure
among the cases reflects the increased risk
that exposure confers
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44. Case-Control Studies: Strengths
• Good for rare outcomes: cancer
• Can examine relation of exposures to disease
• Useful to generate hypothesis
• Fast
• Cheap
• Provides Odds Ratio
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45. Case-Control Studies: Weaknesses
• Cannot measure
– Incidence
– Prevalence
– Relative Risk
• Can only study one outcome
• High susceptibility to bias
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46. Cohort Study
• Begin with disease-free individuals
• Classify patients as exposed/unexposed
• Record outcomes in both groups
• Compare outcomes using relative risk
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47. Cohort Study
• Other Name Prospective Study / Follow-up Study/Incidence Study
• Unit of Study Individual
• Study Question What is happening
• Direction of Inquiry F O
• Study Design Diseased
•
Exposed to Not Non
Factor Diseased
Cohort
Cohort Diseased
Not
Exposed to
Factor
Non-Diseased
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48. Logic of Cohort Study
Cohort is a group of persons sharing a
common characteristics
Differences in the rate at which exposed and
control subjects contract a disease is due to
the differences in exposure, since others are
known and similar.
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49. Cohort Study
Prospective (usually)
Controlled
Can determine causes and incidence of
diseases as well as identify risk factors
Generally expensive, time consuming and
difficult to carry out
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50. Steps for Cohort Study
Identify geographically defined group
Identify exposed subjects and not exposed
subjects
Follow over a specific time
Record the fraction in each group who
develop the condition of interest
Compare these fractions using RR, AR or OR
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51. Objectives of a Cohort Study
To find out association
To assess Risk Ratio
To find out Relative Risk
To find out Attributed Risk
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52. Prospective Cohort Study
DM
No Meditation
No DM
Cohort
DM
Regular
Meditation No DM
Present Future
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53. Cohort Study: Strengths
• Can measure multiple outcomes
• Can adjust for confounding variables
• Can calculate Attributed Risk
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54. Cohort Study: Weaknesses
• Expensive
• Time consuming
• Cannot study rare outcomes
• Confounding variables
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55. Measurements of association
Cohort Study Case Control Study
•Significance Test •Significance Test
•Relative Risk •OR
•Attributable Risk
•OR
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56. Measures of Association
Significance Test – to test significance of
difference in exposure between control and
Cases
Odds ratio - ratio of the odds of contracting
disease in given exposure
Relative Risk – Ratio between incidence
among exposed and incidence among non-
exposed
Attributed Risk – percentage of difference
between incidence among exposed and non-
exposed with incidence among exposed
RR or OR of 1 indicate no effect of exposure (equal odds)
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57. ‘Z’ Score of Exposure Rates
Cases control
Exposed a b
a x 100
Exposure Rates = in Cases Non- c d
exposed
(P2) a+c
b x 100
Exposure Rates = in Controls P2 – P1
(P1) b+d Z Score =
SEDP
P1 Q 1 P 2 Q 2
SEDP = ------------- + --------
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N1 N2
58. ad
ODD‟s Ratio = Times
bc
Incidence among Exposed
RR = Times
Incidence among Non-Exposed
a/a+b a (c+d)
= =
c/c+d c (a+b)
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59. Attributed Risk
(Incidence among Exposed - Incidence among Non-Exposed)
AR = x 100
Incidence among Exposed
a
Incidence among Exposed= x 100
a+b
c
Incidence among Non-Exposed= x 100
c+d
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60. Experimental Studies
Clinical trials provide the “gold standard” of
determining the relationship between factor
and the event
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61. Types of Experimental Study
As per Randomization:
• Randomized Control Trials (RCT)
• Concurrent Parallel Design (RCT)
• Sequential RCT Design
• RCT with External Control
• Non – Randomized Trials (NRCT)
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62. Types of Experimental Study….
As per Design:
• Simple
• Cross-Over Study Design
As per Study Area:
• Field Trials
• Clinical Trials
• Lab. Trials
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63. Quality of Experimental Study
• Randomization
• Blinding
• Control
• Cross-Over
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64. Controls in Clinical Trials
A clinical trial is a comparative, prospective
experiment conducted in human subjects
• Historical controls are better than no
controls
• Patients can serve as own controls - This is
usually beneficial as the comparison
removes patient differences
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65. Blinding
Good practice: factors that can affect the
evaluation of outcome should not be permitted
to influence the evaluation process
Single-blind
Patient or evaluator (either of one) is blinded as
to intervention
Double-blind design
Neither patient nor outcome evaluator knows Rx
to which patient was assigned
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66. Randomized Control Trials (RCT)
• Before and After Comparison
• Comparison with Placebo
• Comparison Of two medicine/procedure/tests
• Comparison Of > two medicine/procedure/tests
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67. Experimental Study
• Other Name Intervention Study
• Objective To know the effect of intervention
• Unit of Study Individual meeting entry criteria
• Study Question What is happening after intervention in
both groups
• Direction of Inquiry I E
• Study Design 1(Intervention with Placebo) Positive
Outcome
Group 1/cases Intervention
Negative
Outcome
Positive
Outcome
Group
Placebo
2/control
Negative
Outcome
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68. Clinical Trial
R Treatment
a Outcomes
Group
n
d
Study o
Population m
i
z Outcomes
e Control Group
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69. Intervention Study - Design 2
(Comparison of Effect of Two Interventions)
Cases
Meeting
Entry criteria
Group - 1 Group -2
Intervention -1 Intervention Intervention - 2
Positive Negative Positive
Outcome Negative
Outcome Outcome Outcome
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70. Cross Over Design
Group -1 Cases Group-2
Meeting
Entry
criteria Intervention - 2
Intervention - 1
Positive Negative
Positive Negative Outcome
Outcome Outcome
Outcome
Group -1
Group -2 Crossover
Intervention -2
Intervention -1
Positive Negative
Positive Negative
Outcome
Outcome Outcome
Outcome
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71. Other Types of Experimental Study
• Quincy Experimental Study
• Block Experimental Study
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72. Quincy Experimental Study
Cases
Meeting
Entry criteria
Group - 1 Group -2
Intervention Intervention No Intervention
Positive Negative Positive
Outcome Negative
Outcome Outcome Outcome
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73. Block Experimental Study
Cases
Meeting
Entry criteria
Group -3
Group - 1
Group -2
Intervention Intervention-3
Intervention -1 Intervention
Intervention-2
Positive Positive Negative
Negative
Outcome Outcome Outcome Outcome
Positive Negative
Outcome Outcome
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74. Steps of Experimental Study
Drawing up a Protocol
Reference Population
Sample Population
Exclusions
Randomization
Experimental Group Control Group
Manipulation/Intervention
Follow - up
12/08/2012 Assessment of Outcome
Dr. Kusum Gaur 74
76. STUDY QUESTIONS AND APPROPRIATE DESIGNS
Type of Question Appropriate Study Design
Burden of illness Field Surveys
- Prevalence Cross Sectional Survey
- Incidence Longitudinal survey
Causation, Risk & Prognosis Case Control Study,
Cohort study, RCT
Treatment Efficacy Randomized Controlled study
Diagnostic Test Evaluation Randomized Controlled study
Cost Effectiveness Randomized Controlled study
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77. Hierarchy of Epidemiological Study Design
Establish Causality RCT
Cohort
Case Control
Cross-Sectional
Case Series
Generate Hypothesis Case Report
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78. Methodology
Study Area : Location of study - Hospital, community
etc.
Study Period: Start to end of Study (maximum period
available for study should be defined)
*Selection of Study Design
* Selection of Study Population
Sample Size
Sampling Technique
Pre-requisits of study: Study Tools, Terminologies,
Orientation trainings etc.
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79. Selection of study population
Whole Population
Sample Population
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80. What is Sample ?
• A sample is a small representative
segment of a population
• Inferences drawn from a sample are
expected to be applicable for the source
population
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81. Why do we need a sample?
To get inferences
applicable to universe
with minimum resources
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82. Sample – Qualities
Sample is a part of population but it is true
representative of whole.
Qualities
Adequate size
Appropriate sampling technique
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83. Factors on which SAMPLE SIZE depend:
• Population Factors
– Type of information available
• Type of study
– Type of Data
– Type of study design
– Type of sampling
– Type of Statistical Analysis for outcome needed
• Determined values of research by researcher
– Power
– Significance level
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84. Power: Ability to detect right answer
Alpha Error: Chance to miss right answer
85. Type of Data & level of Measurements
Qualitative – Counted Facts – Nominal Data
Measured as Numbers expressed as proportions
Quantitative- Measured Facts - Numerical Data
Measured as quantity & expressed as Mean SD
*Ordinal Data – Rank Order Data
Measured as rank & expressed as Median Percentile
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86. Sample size for Qualitative data
Z 2 PQ 4 PQ
Sample Size= ------------------- -- = ------------------
L2 L2
P= Prevalence of disease
Q = 100-P
L = allowable error
Z= 1.96 ≈ 2 for 95% CL
for descriptive/case-series type of study design
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87. Sample size for Quantitative data
Z 2 SD 2 4 SD 2
Sample Size= ------------------- -- =----------------------
L2 L2
SD= Standard Deviation
L = allowable error
Z= 1.96 ≈ 2 for 95% CL
For Descriptive Studies only
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88. Finite Correction
Sample Size – Finite Population (where the
population is less than 50,000)
SS
New SS = _________________
( 1 + ( SS – 1 ))Pop
89. How many controls?
n
k Here n0=No. of cases &
2n0 n n = expected no. of cases
• k = 13 / (2*11 – 13) = 13 / 9 = 1.44
• kn0 = 1.44*11 ≈ 16 controls (and 11 cases)
– Same precision as 13 controls and 13 cases
90. Sampling Design factors of sample size
Variance of Specified Sampling
Design Effect =
Variance of Simple Random Sampling
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91. Sampling Technique effect on Sample Size
Sampling Technique Design Effect Size Multiplier
Simple Random Sampling 1
Systemic Random Sampling 1.2
Stratified Random Sampling 0.8
Cluster Random Sampling 2
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92. Conventionally accepted
Researcher’s Estimations
Alpha Error 0.05
Power 80%
Confidence Limit 95%
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93. Key Concepts: Sample size
• Sampling Design - larger sample for Custer
• Desired Power – more power for larger sample
• Allowable error – smaller error for larger sample
• Heterogeneity leads to have larger sample to
cover diversities
• Nature of Analysis – Complex multivariate
needs larger sample
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94. Steps -Sample Size Estimation
• Stage 1- * Base Sample Size Calculation (n)
• Stage 2 – Sample Size with Design Effect (d)
=n*d
• Stage 3- Contingency Addition (e.g. 5%)
SS Estimation for study population
=(n*d)+5%of n
*Use appropriate equation for sample size
calculation
http://stat.ubc.ca/~rollin/stats/ssize/
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95.
96.
97. E.G. Mean 1= 5, Mean 2 = 15 & SD = 14 inputting values
113. Random sampling Techniques
Aim is to give equal chance to
every observation unit to be
selected for study in sample.
(Any Observation unit
should not have Zero Probability )
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114. * Random Sampling Techniques
Simple Random Technique
Systemic Random Technique
Stratified Random Technique
Multiphase Random Technique
Multistage Random Technique
Cluster Random Technique
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115. Simple Random Technique
• Lottery Method
• Random Table Method
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117. Steps –Use of Random Table
• Stage 1- Give number to each member of population
• Stage 2 – Determine total population size (N)
• Stage 3- Determine Sample size (S)
• Stage 4 – Drop one finger on Random Table with eyes
closed
• Stage 5 – Drop one finger with eyes closed on direction
to be chosen – Up/Down/Rt/Lt
• Stage 6- Determine first number within 0 to N
• Stage 7- * Determine other numbers till Sample size (S)
* Once a number is chosen do not repeat it again
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118. Steps –Use of Random Table..
e.g. N=300, M=50
Random no. Selected no. (3 digits from 0-300)
49468
49699
14043 043
15013 013
12600
33122 122
94169 169
89916
74169 169
32007 007
www.evaluation
wikiog/index/how_to_use_a_random_number_Table
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119. Systemic Random Technique
The selection of sample follows a systematic
interval of selection
• Find serial interval
(K) = total population/sample size
• 1st observation through simple random sampling
among 1to K. th
• Next observation = (1st +K) Observation
• Next observation = (2 nd +K)th Observation
• -------------so on till No. of observations
= Sample Size
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120. Systemic Random Technique Population
N=100 (Given) 1 21 41 61 81
2 22 42 62 82
S=20 (Estimated) 3 23 43 63 83
K=N/S =100/20 =5 4 24 44 64 84
5 25 45 65 85
1st observation between 1 to 5 6 26 46 66 86
7 27 47 67 87
though SRS e.g. 3 8 28 48 68 88
Every 5th observation from 3rd 9 29 49 69 89
10 30 50 70 90
observation will be included in 11 31 51 71 91
sample population 12 32 52 72 92
13 33 53 73 93
So, sample population will be – 3rd 14 34 54 74 94
8th 13th 18th 23rd 28th 33rd 38th 15 35 55 75 95
16 36 56 76 96
43rd 48th 53rd 58th 63rd 68th 73rd 17 37 57 77 97
78th 83rd 88th 93rd and 98th 18 38 58 78 98
19 39 59 79 99
observation 20 40 60 80 100
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121. Stratified Random Technique
Sample selection through Simple Random/Systemic Random Technique
Sample Strata 1
Sample
Strata 2
Sample Strata 3
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122. Multiphase Random Technique
Specific test
Screening Test
S/S
Population
Probable cases Cases
Suspected cases For
study
12/08/2012 Dr. Kusum Gaur 129
123. Multistage Random Technique
Each stage Simple RT is used village
district
village
village
State 1 district
Population village
Study
Of Population
Nation village
district
village
State 2
village
district
village
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124. Cluster Random Technique
The unit of random selection is a cluster rather than individual
• CI = Total population /30 (in 30 Cluster Technique)
Cluster 1 Cluster 27
Cluster 2 Cluster 28
Population Study
Of Population
Nation Cluster 3 Cluster 29
Cluster 30
Cluster 4
Through Simple RT
12/08/2012 Dr. Kusum Gaur 131
125. Stratified Vs Cluster Technique
Stratified Technique Cluster Technique
• Homogenous groups • Comparable groups of
are made population are made
• Randomly select (usually 30)
sample from each • Randomly select
group
sample from each
• To make it more truly group
representative, take
sample population • More chances of error
proportion to size (PPS) than simple random
• Less chances of error
than simple random
126. Non Probability Sampling
• When random samples are not possible
• Rare disease
• Small population
• Special population
• Special Condition
• Difficult to reach population
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127. Non-probability Samples
Convenience
Purposive
Quota
Snow ball study
12/08/2012 Dr. Kusum Gaur 134
131. Snow ball sampling
Contact tracing
Initial respondent helps in recruiting
new population
Useful in network analysis approach
12/08/2012 Dr. Kusum Gaur 138
132. Step-4 & 5
Data Collection
and
Data Management
133. Sources of Data
• Primary –Own generated data
• Secondary –Already generated data
Published
Non-Published
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134. Primary Vs Secondary source of Data
Primary data Secondary data
• Need to be generated • Readily available
• First hand information • Second hand information
• Questionnaire
• Not need of questionnaire
• Purpose served
• Purpose served ?
• Analysis as per
purpose
• Require more time and • Descriptive
money • Less expensive
12/08/2012 Dr. Kusum Gaur 141
135. Type of Data Collection Methods
Interview
Personnel
Telephonic
Observation
Experimental
Interview and Observation
Observation and Experimental
Interview ,Observation and Experimental
12/08/2012 Dr. Kusum Gaur 142
136. Forms of questions(Open Vs Closed)
Open ended Close ended
• Possible responses are • Categories are given
not given. already coded
• Mean, SD, Median • Proportion
• For seeking opinions, • For eliciting factual
attitudes ,perceptions information
• Not so depth
• Provides in depth info. • Investigator‟s bias
• Experience of • Ease of answering,
investigator and • Easy to analyse
analyst required
12/08/2012 Dr. Kusum Gaur 143
137. Considerations in formulating questionnaire
(Questionnaire/Interview schedule)
Use simple and everyday language
Do not use ambiguous questions(?/?)
Do not ask leading questions
The order of questions:
Guideline for filling an instrument, pen-pencil
Pre testing
12/08/2012 Dr. Kusum Gaur 144
138. Validity of a Research Instrument
Ability of an instrument to measure what it is
designed to measure being measured
Establish the logical link between the
questions and objectives
Items/questions cover the full range of
issue/attitude being measured
12/08/2012 Dr. Kusum Gaur 145
139. 1.Decide the information required.
Steps
2. Define the target respondents.
3. Method(s) of reaching target
4. Decide on question content.
5. Develop the question wording.
6. Put questions into a meaningful order.
7. Check the length of the questionnaire.
8. Pre-test the questionnaire.
9. Develop the final survey form
12/08/2012 Dr. Kusum Gaur 146
140. Organization and Compilation of Data
Organization and Compilation of Data in such a way
(Master Chart ) to have reliable, relevant, adequate
and reasonably complete data with following
requisites –
Simplicity
Briefness
Utility
Distinctively
Comparability
Scientific Arrangement
Attractive
12/08/2012 Effective
Dr. Kusum Gaur 147
161. Tabulation – Content of Table
Table No. Sequence in the text
Tile of Table –short, clear and self explanatory to say about for
what the table is ?
Body of Table –consist of rows and columns
Rows – 1st row shows headings of columns
1st column shows headings of rows
rest of rows and columns are showing data as per required
number of rows and columns should be limited to maintained
simplicity of table
source of data ( if it is other than the present study ) should be
written just below the body of table
Source of Data ?
Foot Note - written just below the body of table, if there is any
hidden information
Inferences –summary value of table
12/08/2012 Dr. Kusum Gaur 168
162. Types of Tables
As per purpose
General tables –about Socio-demographic profile
Specific tables –about Aims and objectives
As per originality
Original tables-from original Data
Derived tables –from original tables
As per Construction
Simple tables- showing one variable at one time
Complex tables – showing > one variable at one time
12/08/2012 Dr. Kusum Gaur 169
169. Multiple Bar diagram
60
50
40
(1) 1-5 Years
30
(2) 6-10 Years
(3) 11 & Above Years
20
10
0
(1) Very Dissatisfied (2) Dissatisfied (3) neither satisfied (4) Satisfied (5) Very Satisfied
nor dissatisfied
12/08/2012 Dr. Kusum Gaur 176
171. Pie diagram
Propotion of Pie = (Proportion of that variable )(360)Degree
12%
14% 1st Qtr
2nd Qtr
82% 3rd Qtr
4th Qtr
32%
12/08/2012 Dr. Kusum Gaur 178
172. Line diagram
7
6
5
4
Series 2
3
Series 1
2
1
0
2000 2001 2002 2003 2004 2005
12/08/2012 Dr. Kusum Gaur 179
173. Histogram ( Area Diagramme)
Series 1
40
30
20
10
Series 1
0
0 to 5 yrs
5yrs to 10
10 yrs to
yrs 15 yrs to
15 yrs 20 yrs to
20 yrs
25 yrs
12/08/2012 Dr. Kusum Gaur 180
174. Scatter Diagram
30
25
20
Duration of Diabetes
15
Duration of diabetes in yrs.
Linear (Duration of diabetes in yrs.)
10
5
0
0 50 100 150 200 250 300
No. of Patients
12/08/2012 Dr. Kusum Gaur 181
175. Radar diagram
5/1/2002
40
30
20
9/1/2002 6/1/2002
10
Series 1
0
Series 2
8/1/2002 7/1/2002
12/08/2012 Dr. Kusum Gaur 182
176. Box & Whisker
70
60
50
40 Open
High
30 Low
20 Close
10
0
5/1/2002 6/1/2002 7/1/2002 8/1/2002 9/1/2002
12/08/2012 Dr. Kusum Gaur 183
178. Biostatistics = Biology + Statistics
• Biostatistics is application of statistics in
biology i.e. science of figure in medical science
• Data: Set of information, facts or figures
numerically coded and from which conclusions
may be drawn is called data (singular-datum).
• Statistics: The collection of methods used in
planning an experiment
and analyzing data in order to draw accurate
conclusions.
179. Type of Biostatistics
• Descriptive statistics generally characterizes
or describes a set of data elements
• Inferential statistics tries to infer information
about a population by using information
gathered by sampling
180. Descriptive Analysis
Qualitative Data
Rates
Ratios
Proportions
Quantitative Data
Central Tendencies Disperson
Mean Standard Deviation
Mode Standard Error
Median Confidencial Limit
Skeweness
12/08/2012 Dr. Kusum Gaur 187
181. Descriptive Analysis of
Qualitative Data
No. of total Events in a year (A)
Rate = * 1000
MYP of that Region (T)
No. of total (A)
Ratio =
No. of total (B)
No. of Specific Events (A)
Percentage of Events = * 100
Total Events (T)
Event of Sp. Cause (A)
Proportional Rate = * 10 n
Total Deaths (T)
12/08/2012 Dr. Kusum Gaur 188
182. Descriptive Analysis of
Quantitative Data
Mean = Mathematical Average ∑X
N
Mode = Most commonly occurring value
Median = Center value when arrange in increasing N+1
or decreasing fashion 2
Standard Deviation = It tells how much scores deviate from the mean
it is the square root of the variance
it is the most commonly used measure of spread (X-X)
SD=√ N
Standard Error = Deviation from mean per observation
SD/ √N
Skewness = Deviation of peak from median
SK= 3 (Mean –Median)/SD
12/08/2012 Dr. Kusum Gaur 189
186. TEST OF SIGNIFICANCE OF QUALITATIVE DATA
TEST OF SIGNIFICANCE OF QUALITATIVE DATA
One Sample Two Sample >Two Sample
Sample proportion
to Independent Dependent Dependent Independent
Population Proportion
Mc Numer Cochron’s
Large Sample Small Sample
(>30) (<30)
Small Sample Large Sample Large Sample Small Sample
Yat’s Corrected
‘Z’ Score Corrected ‘Z’ Score Chi Squire
Chi Squire ‘Z’ Score Chi Squire
Yat’s Corrected Chi
Chi Squire
12/08/2012 Dr. Kusum Gaur 193
187. TEST OF SIGNIFICANCE OF QUANTITATIVE DATA
TEST OF SIGNIFICANCE OF QUANTITATIVE DATA
One Sample Two Sample >Two Sample
Sample Mean
to Independent Dependent Dependent Independent
Population Mean
Paired ‘T’ Test ANOVA Friedman
Large Sample Small Sample
(>30) (<30)
Small Sample Large Sample Large Sample Small Sample
‘Z’ Test ‘T’ Test
‘Z’ Test ANOVA ANOVA
12/08/2012 Dr. Kusum Gaur 194
188. STUDY DESIGNS AND APPROPRIATE TEST
Type Study Design Appropriate Significance Test
Descriptive Study
Analytical
Case Control Study OR
Qualitative ‘Z’ Score Test/Chi-Square Test
Quantitative ‘Z’ Test/’t’ Test
Cohort study OR, AR, & RR
Qualitative ‘Z’ Score Test/Chi-Square Test
Quantitative ‘Z’ Test/’t’ Test
12/08/2012 Dr. Kusum Gaur 195
189. STUDY DESIGNS AND APPROPRIATE TEST
Type Study Design Appropriate Significance Test
Randomized Controlled study
Quantitative (before and after)- Paired ‘t’ Test
Quantitative (before and after >1 followup)- Freidmen ANOVA
Quantitative (between two Gps)- Unpaired ‘t’ Test
Quantitative (between > two Gps)- ANOVA Test
Randomized Controlled study
Qualitative (before and after)- Mac Numer Test
Qualitative (before and after >1 followup)- Cochron’s Test
Qualitative (between two Gps)- ‘Z’ Score/Chi-square Test
12/08/2012 Qualitative (between > two Gps)- Chi-square Test
Dr. Kusum Gaur 196
190. STATISTICAL TEST OF SIGNIFICANCE
Nominal Numerical Ordinal
Two Groups ‘Z’ Score Test ‘Z’ test (n>30) Mann Whitny
Chi-square Test T Test (n<30)
> Two Groups Chi-square Test ANOVA Kruskal Wallis
Paired Two Mec Numer Paired ANOVA Wilcoxon Sign
Multiple Cohrane Repeated Friedman
Observation in Multivarient ANOVA
same individual
Association of Contegency Correlation(Pearson) Spearman
Two Variable Cofficient Regression Correlation
191. STATISTICAL TEST OF SIGNIFICANCE
Research Number and Number and Covariates Test Goal of Analysis
Question type of DV type of IV
Nominal 1 nominal chi square determine if difference between
Group croups
differences Continuous 1 dichotomous t-test
Determine significance of
1 Categorical 1 one-way ANOVA mean group
1+ one-way differences
ANCOVA
2+ Categorical 1 factorial ANOVA
1+ factorial ANCOVA
2+ Continuous 1 Categorical 1 one-way MANOVA Create linear
1+ one-way MANCOVA combo of Dependent variable
2+ Categorical 1 factorial (Dvs)
MANOVA to maximize
1+ factorial MANCOVA mean group
differences
Degree of Continuous 1 Continuous Bivariate Determine relationship/prediction
relationship Correlation
2+ Continuous Multiple Linear combination to predict the
Regression DV
1+ Continuous 2+ Continuous Path Analysis Estimate causal relations among
variables
12/08/2012 Dr. Kusum Gaur 198
192. Comparing difference between
Two Sample Proportions
„Z‟ Score Test
P2 – P1 here, P1– proportion of that event in 1st Sample
„Z‟ Score = P2 - proportion of that event in 2nd Sample
SEDP SEDP – Standard Error of
Difference in Proportion
Q1 - proportion without that event
in 1st Sample i.e. Q1 = 100 – P1
Q2 - proportion without that event in
P1 Q 1 P 2 Q 2 2nd Sample i.e. 100 – P2
SEDP = ------- + -------- N1 - Sample Size of 1st Sample
N1 N2 N2 - Sample Size of 2nd Sample
12/08/2012 Dr. Kusum Gaur 199
193. Inference of ‘Z’ Score Test
If „Z‟ > 2 = Difference is Significant
If „Z‟ < 2 = Difference is Not Significant
If „Z‟ > 3 = Difference is Highly Significant
12/08/2012 Dr. Kusum Gaur 200
194. Comparing difference between
>Two Sample Proportions
Chi-Square Test
Indications
Qualitative data
Normal distribution
Comparing difference between
Two Sample proportions
Multiple Sample proportions
12/08/2012 Dr. Kusum Gaur 201
195. Comparing difference between
>Two Sample Proportions
Chi-Square Test
Chi Square(2) = ∑all cells(O-E)2 Tr x Tc
E=
E T
(O1-E1)2 (O2-E2)2 (O3-E4)2 (On-En)2
Chi Squire = + + + ---+
E1 E2 E3 En
Tr – Total of that Row
here, O – Observed value of cell
Tc – Total of that column
E – Expected value of cell,
T – Grand Total i.e. a+b+c+d
considering Null Hypothesis
Degree of Freedom (DF) = (C – 1) (R -1)
R= No. of Rows, C = No. of Column
12/08/2012 Dr. Kusum Gaur 202
196. Inference of Chi Square(x2)
Chi Square(x2 ) value is seen at Degree of Freedom
DF = (R – 1) (C – 1), from Chi Square((2) Table
(here R=No. of Rows &C= No. of Column)
at desired level of significance
Inferences
If Chi Square(x2 ) Test Value is –
Higher than Table value = Difference in proportions is
Significant at that desired level of significance.
If Chi Square(x2 ) Test Value is –
Lower than Table value = Difference in proportions is
Not Significant at that desired level of significance.
12/08/2012 Dr. Kusum Gaur 203
197. Comparing difference between
Two Sample Means (>30)
„Z‟ Test
Pre-requisites
Quantitative data
Homogenous normally distributed Random Sample
Sample Size > 30
Indications
To see the Significance of any Observation in
reference of Mean Value of that sample
Comparing difference between
Sample Mean to Population Mean
Means of Two independent Samples
12/08/2012 Dr. Kusum Gaur 204
198. Comparing difference between
Two Sample Means (>30)
„Z‟ Test
X2 – X1 here, X1– Mean of that event in 1st Sample
„Z‟ Test = X2 - Mean of that event in 2nd Sample
SEDM SEDM – Standard Error of
Difference in Means
SD1 – Standard Error of 1st Sample
SD2 – Standard Error of 2nd Sample
N1 - Sample Size of 1st Sample
SD2 1 SD2 2 N2 - Sample Size of 2nd Sample
SEDM = ------- + --------
N1 N2
12/08/2012 Dr. Kusum Gaur 205
199. Comparing difference between
Two Sample Means (<30)
„T‟ Test
Prerequisites
Random Sample
Quantitative data
Normally Distributed
Sample Size < 30
12/08/2012 Dr. Kusum Gaur 206
200. Type of ‘T’ Test
as per design
Unpaired / Paired
for inference
One Tail /Two tail
12/08/2012 Dr. Kusum Gaur 207
201. Unpaired ‘T’ Test Design
Population -1 Population -2
S-1 S-2
Mean --1 Unpaired ‘T’ test Mean --2
12/08/2012 Dr. Kusum Gaur 208
202. Paired ‘T’ Test Design
Intervention
Population Sam
Observations-1 Observations 2
ple-
Mean --1 Mean --2
Paired ‘T’ test
12/08/2012 Dr. Kusum Gaur 209
203. One Tail ‘T’ Test
Acceptance Zone Rejection Zone
One Tail – Results are aspect only in one direction
204. Two Tail ‘T’ Test
Rejection Zone Acceptance Zone Rejection Zone
Two Tail – Results are aspect in both direction
205. Comparing difference between
Two Sample Means (<30)
„T‟ Test
X2 – X1 here, X1– Mean of that event in 1st Sample
„T‟ Test = --------------- X2 - Mean of that event in 2nd Sample
SEDM SEDM – Standard Error of
Difference in Means
SD1 – Standard Error of 1st Sample
SD2 – Standard Error of 2nd Sample
N1 - Sample Size of 1st Sample
SD2 1 SD2 2 N2 - Sample Size of 2nd Sample
SEDM = ------- + --------
N1 N2
Degree of Freedom (DF) = (N1 – 1) + (N2 -1) = N1 + N2 - 2
12/08/2012 Dr. Kusum Gaur 212
206. Inference of ‘T’ Test Value
„T‟ Test Value is matched at Degree of Freedom
(DF) = N1 + N2 – 2 in the Table of “T”
at desired level of significance.
Inferences
If „T‟ Test Value is –
Higher than Table value = Difference in Means is
Significant at that desired level of significance.
If „T‟ Test Value is –
Lower than Table value = Difference in Means is
Not Significant at that desired level of significance.
12/08/2012 Dr. Kusum Gaur 213
207. Comparing difference between
>Two Sample Means
ANALYSIS OF VARIENCE (ANOVA) TEST
Pre-requisites
Quantitative data
Homogenous normally distributed Random
Sample
Indications
Comparing difference between more than Two
Means
12/08/2012 Dr. Kusum Gaur 214
208. Comparing difference between
>Two Sample Means
„ANOVA‟ Test
MSOSI MSOS2 - Mean Sum Of Squares Within Classes
ANOVA = ---------- = Total SOS – MSOSI
MSOS2
T SOS = X2 – (X)2/N
MSOSI – Mean Sum Of Squares Between Classes = SOSI / K-1
SOSI –Sum Of Squares Between Classes
(Xa)2 (Xb)2 (Xc)2 (Xk)2 (X)2
= --------- + ----------- + ----------- + ….+ ____ __ - ---------
Na Nb Nc Nk N
At Degree of Freedom (DF) = ( K-1) Horizontal
12/08/2012 Dr. Kusum Gaur
(N – K) Vertical
215
209. Inference of ANOVA
Find out Variance Ratio value at Degree of Freedom
(DF) = ( K-1) Horizontal, (N – K) Vertical
from the Variance Ratio Table
at desired level of significance.
Inferences
If Test value is > Table value = Difference in Means is
Significant at that desired level of significance.
If Test value is < Table value = Difference in Means is
Not Significant at that desired level of significance.
12/08/2012 Dr. Kusum Gaur 216
211. Type & Degree of Correlation
Correlation Inference Correlation (r) Inference
+1 Perfect +ve -1 Perfect +ve
Correlation Correlation
> 0.95 About Perfect +ve > - 0.95 About Perfect +ve
Correlation Correlation
> 0.75 V. Good Correlation > - 0.75 V. Good Correlation
0.75 – 0.5 Moderate Correlation - 0.75 to – 0.5 Moderate
Correlation
0.5 – 0.25 Fair Correlation - 0.5 to – 0.25 Fair Correlation
0.25 - 0 No Correlation < - 0.25 No Correlation
12/08/2012 Dr. Kusum Gaur 218
212. Correlation
CORRELATION
Two Variables > Two Variables
Un-Paired Data Paired Data
Pearson‟s Spearman‟s Rank Order Multivariate
Correlation Correlation Correlation
12/08/2012 Dr. Kusum Gaur 219
213. Pearson’s correlation
. ∑ ( X – X) ∑ ( Y – Y) ∑xy
Correlation (r) = =
√∑ ( X – X)2 ∑ ( Y – Y)2 √ ∑ x2 y2
Direct Method
∑ X Y - ∑ X ∑Y / N
Correlation (r) = -----------------------------
√ {∑X2 – (∑X)2/N}{ ∑Y2 – (∑Y)2 /N}
12/08/2012 Dr. Kusum Gaur 220
214. Pearson’s correlation -----
here,
∑ X Y = Sum of multiplication of X and Y
∑ X = Sum of all observations of X Series
∑ Y = Sum of all observations of YX Series
N =Total no. of observations
∑X2 = Sum of Squares of all observations of X Series
∑Y2 = Sum of Squares of all observations of Y Series
(∑X)2 = Square of Sum of all observations of X Series
(∑Y)2 = Square of Sum of all observations of Y Series
12/08/2012 Dr. Kusum Gaur 221
215. Spearman’s Rank Order Correlation
6∑D2
• Spearman‟s Rank (rs ) = 1 -
N3 - N
12/08/2012 Dr. Kusum Gaur 222
216. Significance Test for Correlation (r)
Standard Error (SE) of rs = rs √ N-1
Inference
• If difference >2 SE of r =Difference is
Significant at 5% level
• If difference < 2SE of r =Difference is
Not Significant at 5% level
12/08/2012 Dr. Kusum Gaur 223
217. REGRESSION
Indication
To find out causal relationship between
variables
REGRESSION COFFICIENT- It is a measure of
change in one dependent variable (y) with
one unit change in the other variable (x)
12/08/2012 Dr. Kusum Gaur 224
218. Regression line with Regression Equation
The regression equation of ‘Y’ on ‘X’ is expressed as follows:
Here, ‘a’ is interceptor & ‘b’ is slope Yc = a + bX
219. Regression Lines
Régression line of Y on X is Y = a + bX ----(1)
Régression line of X on Y is X = a + bY ----(2)
Here- Y = one variable
X = other variable
a = interceptor of X line on Y line
b = slope of X line on Y line Regression
12/08/2012 Dr. Kusum Gaur 226
220. Regression – Equations
Regression Equation of X on Y
SD of series X
(X – X)= r (Y –Y) ---- (3)
SD of series Y
Regression Equation of Y on X
SD of series Y
(Y – Y)= r (X –X) ------- (4)
SD of series X
12/08/2012 Dr. Kusum Gaur 227
221. Regression – coefficients
Regression Coefficient of X on Y
SD of series X ∑(X-X)(Y –Y)
b(xy)= r =
SD of series Y ∑(X – X)2
Regression Coefficient of Y on X
SD of series Y ∑(X-X)(Y –Y)
b(yx)= r =
12/08/2012
SD of series Kusum Gaur
Dr.
X ∑(Y – Y)2 228
222. Relation of correlation and
Regression
Co-rrelation (r) = √ bxy byx
12/08/2012 Dr. Kusum Gaur 229
223. Between
Tests/Procedure/Therapy
For comparison with Gold Standard:
Sensitivity
Specificity
PPV
NPV
ROC
For agreement of association: Kappa
For appropriate cut of value for diagnostic test: ROC
12/08/2012 Dr. Kusum Gaur 230
224. Sensitivity and Specificity
Status based on gold standard test
Diseased Normal
Test positive True positive False positive
Observation in a b
new test Test negative False negative True negative
c d
Sensitivity = a /(a+c) PPV = a /(a+b)
Specificity = d /(b+d) NPV = d /(c+d)
12/08/2012 Dr. Kusum Gaur 231
226. Kappa Statistics
(Measurement of Agreement)
Test Value Inference
0.93 – 1 Excellent Agreement
0.81 – 0.92 Very Good Agreement
0.61 – 0.80 Good Agreement
0.41 – 0.60 Fair Agreement
0.21 – 0.40 Slight Agreement
0.01 – 0.20 Poor Agreement
< 0.01 No Agreement
12/08/2012 Dr. Kusum Gaur 233
227. Non-Parametric Tests
Advantages
Distribution free
Easier to do
Easier to understand/infer
Disadvantages
They ignore certain amount of information
Indicated only ordinal or nominal data
Statistically Less efficient
Indicated only to test hypothesis, not for
estimates
12/08/2012 Dr. Kusum Gaur 234
228. Parametric Test Vs Non-Parametric
Test Quality Parametric Non-Parametric
Assumed Distribution Normal Any
Assumed Variance Homogenous Any
Data Type Interval-Continous Nominal /Ordinal
Data set Relationship Independent Any
Usual Centre Measure Mean Median
More conclusions Easier to calculate
Advantages
More efficient Less affected by outliers
12/08/2012 Dr. Kusum Gaur 235
229. Parametric Test Vs Non-Parametric
Test Parametric Non-Parametric
Correlation test Pearson Spearman
Independent Independent-
Mann-Whitney test
measures, 2 groups measures t-test
One-way,
Independent
independent- Kruskal-Wallis test
measures, >2 groups
measures ANOVA
Repeated measures,
Matched-pair t-test Wilcoxon test
2 conditions
Repeated measures, One-way, repeated
Friedman's test
>2 conditions measures ANOVA
Sign Test (K Test)– nonparametric test for quantitative paired data
12/08/2012 Dr. Kusum Gaur 236
230. Sign test
• Simplest
• Based on direction(- /+/0)
• Signs as per the direction are counted
• Inference – if S≤K = Null hypothesis (H₀) is
rejected
• Here „S‟ is net sum of signs as per sign
• „K‟ is constant
12/08/2012 Dr. Kusum Gaur 237
231. Sign test – Steps
Sign K Test for Small Sample (<30)
– Find out net sum of signs as per sign(S)
– S = (total + signs) – (total – signs)
– K = (n-1)/2 - 0.98√n
• Inference – if S≤K = Null hypothesis (H₀) is rejected
Sign Z Test for Large Sample (>30)
– Find out no of ties with less frequent sign(X)
– Z = (X – np) / √ np (1-p) here X= no. + Sign
• Inference – if Z>2 = Null hypothesis is rejected
12/08/2012 Dr. Kusum Gaur 238
237. Step-7
Inferences
12/08/2012 Dr. Kusum Gaur 244
238. Steps in Statistical Inference
Generating NULL and ALTERNATIVE
hypothesis
Testing the hypothesis using appropriate
statistical tests
Obtaining „p‟ value
Concluding from the p value.
Obtaining Level of Significance
Comparing „p‟ value with CI.
12/08/2012 Dr. Kusum Gaur 245
239. ‘P’ Value and Inferences
with Normal Curve
12/08/2012 Dr. Kusum Gaur 246
240. Rejection Zone Acceptance Zone Rejection Zone
Mean 1SD =68% values - Confidence Limit 68% - P Value = >0.05 - NS
Mean 2SD =95% values - Confidence Limit 95% - P Value = 0.05 - S
Mean 3SD =99% values - Confidence Limit 99% - P Value = 0.001 - HS
241. Rejection Zone Acceptance Zone Rejection Zone
Mean 1SD =68% values - Confidence Limit 68% - P Value =/>0.05 - NS
Mean 2SD =95% values - Confidence Limit 95% - P Value < 0.05 – S
Mean 3SD =99% values - Confidence Limit 99% P Value < 0.001 - HS
12/08/2012 Dr. Kusum Gaur 248
242. Conventionally Accepted
Significance Level
P Value > 0.05 LS=Not Significant
P Value < 0.05 LS=Significant
P Value < 0.001 LS=Highly Significant
243. Step-8
Reporting
12/08/2012 Dr. Kusum Gaur 250
244. Steps of Report Writing
Title of Project
Abstract
Introduction
Aims & Objectives
Methodology
Observations-Compilation, Classification &
Presentation of data with analysis and inferences
Discussion
Conclusions
Recommendations
Limitations
Acknowledgment
Bibliography
12/08/2012 Dr. Kusum Gaur 251
245. Discussion
Explanation of findings
Logic and reasoning for the results as it
appears
Compare and contrast with findings of other
researchers
Based on objectives of the study
Should answer the research question
Scope & limitations of the study
12/08/2012 Dr. Kusum Gaur 252
246. Recommendations & conclusions
• Based on our findings
• Limited to objectives of the study
• Policy implications
• Relevance should be emphasized
• Should be exclusively limited to
observations
12/08/2012 Dr. Kusum Gaur 253
247. Managerial and financial aspects
Protocol development
Time line/Gantt chart
Peer review
Development of tools
Training in data collection
Budget/ financial accounting
Quality control
Monitoring & Evaluation
12/08/2012 Dr. Kusum Gaur 254
248. Time Line/Gant chart/log Fram
Activities 1.1.12- 16.1- 1.2.12- 1.3.12- 16.5.12- 16.6.12- 16.7.12-
15.1.12 31.1 15.2.12 15.5.12 15.6.12 15.7.12 31.7.12
Planning
Officials
Que. Dev
Training
Poilet Survey
Corrections
Re-training
Resource Proc
Survey
Analysis
Report Writing
Dissemination
of Report
250. Web sites related to Statistics
• http://stattrek.com
• http://vassarstat.net
• http://www.scribd.com
• http://www.statistixl.com
• http://statistics calculators.com
• http://stat.ubc.ca/~rollin/stats/ssize/
• ………………………………………………………
……
12/08/2012 Dr. Kusum Gaur 257
251. Computer Softwares in Statistics
• Microsoft Excel
• SPSS
• Epi info
• Epi tab
• Mini tab
• Graph Pad
• Primer
• Medcal
• ……………..
12/08/2012 Dr. Kusum Gaur 258
252. Always there is room for improvement
12/08/2012 Dr. Kusum Gaur 259