Chapter 3 tools of the laboratory e mail

Chapter 3 Tools of the Laboratory: The methods for studying microorganisms

Media ,[object Object],[object Object],[object Object],[object Object],[object Object]

5 I’s of microbiology ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

5 I’s of microbiology ,[object Object],[object Object],[object Object],[object Object],[object Object]

5 I’s of microbiology ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

5 I’s of microbiology ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Metric Units 0.0000000001 = 10 -10 Angstrom Å 0.000000001

Scale sizes End light microscope viewing power - Eukaryotic cells are measured in µm (100-10µm) - Bacteria are measured in µm (1-10µm) - Viruses are measured in nm

Try to visualize… ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],How big is a…. Utah Cells’ alive How big?

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Compound Light Microscope

Parts of compound microscope (for lab) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Magnification & Resolution ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Oil immersion ,[object Object],[object Object],[object Object],[object Object],[object Object]

Images with a compound light microscope Bacteria 1000x Amoeba 1000x Note the enormous size difference between one bacterial cell & the amoeba! Bacteria are prokaryotic & these cells are usually much smaller than eukaryotic cells (amoeba)

Finding bacteria with a microscope 100x 1000x 40x 400x

Electron microscope (EM) ,[object Object],[object Object],[object Object],[object Object]

2 types of Electron Microscopes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Virus budding from cell Which is TEM? SEM?

Other types of microscopy ,[object Object],[object Object],[object Object]

Staining ,[object Object],[object Object],[object Object],[object Object],Unstained Stained 40x Stained 100x dust

Staining ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Simple Stain vs Differential Stain ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Gram stain ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Endospore stain ,[object Object],[object Object],[object Object],[object Object],[object Object]

Test yourself! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Chapter 3 tools of the laboratory e mail

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Chapter 3 tools of the laboratory e mail