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BSC 2011L  Photosynthesis Today’s Lab... 1.) Turn in library worksheet.   2.) Quiz # 1  3.) Photosynthesis Lab 4.) Due next wk  -- abstract (15 pts) -- graphs (10 pts) 5.) Quiz #2 next week, Ann teaches
Michelle C 4 Kristen H. 0.5 Allison H.  1 Alex H. 0.5 Michelle H. 5 Dallas J. 2 Sheena M. 2 Michael D.  2 David E. 2.5 Dahlia K. 3.5 Brian M. 2.5 Joseph M. 1 Anna P. 3
3.  What is the difference between a reflex and a reaction?  DRAW AND LABEL A SIMPLE DIAGRAM  to explain your answer. 4.  Close your right eye, look at the cross with your left eye. The circle will disappear. EXPLAIN WHAT IS HAPPENING  5.  In this lab we will be measuring 2 factors that affect the rate of photosynthesis they are A) temperature and light B) chlorophyll A and chlorophyll B C) wavelength and intensity D) DCPIP and NADPH E) glucose and sucrose ,[object Object],[object Object],QUIZ 1 Quiz Bank:  NOTE the number of the question you wish to substitute - DO NOT answer that question!
Cyanobacteria
CYANOBACTERIA 2.7 billion years old
An overview of photosynthesis
L eaves are structured  to facilitate photosynthesis
[object Object],[object Object],[object Object],[object Object],L eaves are structured  to facilitate photosynthesis
The site of photosynthesis in a plant
Chloroplast  structure ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The Photosynthetic Reaction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Tracking atoms through photosynthesis Carbon dioxide   Water Glucose Water Oxygen
Interaction of light with chloroplasts
Light DEPENDENT reactions Photosystem I & Photosystem II
 
 
Light-dependent Reaction ,[object Object],[object Object],[object Object],[object Object],[object Object]
Results of Light-dependent reaction ,[object Object],[object Object],[object Object]
Light independent reaction Carbon Assimilation Reaction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Light independent reaction  –  making the donuts! Light dependent reaction  –  capturing energy from the sun ?
LIGHT REACTION DARK REACTION *note: “dark reactions” = “light independent” reaction = Calvin cycle = carbon fixation    happens in dark and light
What are we doing in this experiment? ,[object Object],[object Object],[object Object],[object Object]
DCPIP used in place of NADP+.
We will look at the effects of two variables on photosynthetic rate: ,[object Object],[object Object],[object Object],[object Object]
“ What is an  Einstein ?” ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The electromagnetic spectrum
Photosynthetic wavelengths
LET’S HYPOTHESIZE... I.) Wavelength II.) Light intensity
Spectrophotometers ,[object Object]
You will prepare  three cuvettes... Why? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
0.5 ml  0.5 ml  0.5 ml  1.7 ml 4.2 ml 1.7 ml wavelength wavelength wavelength What to put in cuvettes...
Make the cuvettes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Thylakoid suspensions are exposed for two (2) minutes, then light can be shut off while absorbencies are measured.
Wavelength experiment ,[object Object],[object Object],[object Object],[object Object],[object Object]
Light intensity experiment ,[object Object],[object Object]
4 figures with captions required ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],2 more graphs 1. reaction rate vs wavelength  2. reaction rate vs. intensity
A  Reaction Rate  versus  distance  graph would be an exponential curve. A  Reaction Rate  versus  intensity  should be linear. Figures ,[object Object],[object Object],[object Object],[object Object],[object Object]
Example of a proper graph... *  Ensure that axes are properly labeled, with units specified, even if there are no units (AU=arbitraty units) * Do not connect the points on the graph with lines; draw a best-fit line through the data set. * The independent variable (the variable you manipulate) should always be on the X-axis and the dependent variable (the read out from the instrument) should be on the Y-axis. * Include a descriptive title that explains what kind of plot it is, the technique that was used, which substance was measured, and what instrument was used to measure it. * IMPORTANT: Titles should not include (or be) "Plot of...", "Graph of...", or "...over a range of data points." - Be concise, but complete. * Do not plot too many data sets on a single graph - make multiple graphs if required. WRITE A DETAILED CAPTION FOR EACH GRAPH see --  http://abacus.bates.edu/~ganderso/biology/resources/writing/HTWtablefigs.html
[object Object],[object Object],[object Object]

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Lab 4 Photosynthesis

  • 1. BSC 2011L Photosynthesis Today’s Lab... 1.) Turn in library worksheet. 2.) Quiz # 1 3.) Photosynthesis Lab 4.) Due next wk -- abstract (15 pts) -- graphs (10 pts) 5.) Quiz #2 next week, Ann teaches
  • 2. Michelle C 4 Kristen H. 0.5 Allison H. 1 Alex H. 0.5 Michelle H. 5 Dallas J. 2 Sheena M. 2 Michael D. 2 David E. 2.5 Dahlia K. 3.5 Brian M. 2.5 Joseph M. 1 Anna P. 3
  • 3.
  • 6. An overview of photosynthesis
  • 7. L eaves are structured to facilitate photosynthesis
  • 8.
  • 9. The site of photosynthesis in a plant
  • 10.
  • 11.
  • 12. Tracking atoms through photosynthesis Carbon dioxide Water Glucose Water Oxygen
  • 13. Interaction of light with chloroplasts
  • 14. Light DEPENDENT reactions Photosystem I & Photosystem II
  • 15.  
  • 16.  
  • 17.
  • 18.
  • 19.
  • 20. Light independent reaction – making the donuts! Light dependent reaction – capturing energy from the sun ?
  • 21. LIGHT REACTION DARK REACTION *note: “dark reactions” = “light independent” reaction = Calvin cycle = carbon fixation  happens in dark and light
  • 22.
  • 23. DCPIP used in place of NADP+.
  • 24.
  • 25.
  • 28. LET’S HYPOTHESIZE... I.) Wavelength II.) Light intensity
  • 29.
  • 30.
  • 31. 0.5 ml 0.5 ml 0.5 ml 1.7 ml 4.2 ml 1.7 ml wavelength wavelength wavelength What to put in cuvettes...
  • 32.
  • 33. Thylakoid suspensions are exposed for two (2) minutes, then light can be shut off while absorbencies are measured.
  • 34.
  • 35.
  • 36.
  • 37.
  • 38.
  • 39. Example of a proper graph... * Ensure that axes are properly labeled, with units specified, even if there are no units (AU=arbitraty units) * Do not connect the points on the graph with lines; draw a best-fit line through the data set. * The independent variable (the variable you manipulate) should always be on the X-axis and the dependent variable (the read out from the instrument) should be on the Y-axis. * Include a descriptive title that explains what kind of plot it is, the technique that was used, which substance was measured, and what instrument was used to measure it. * IMPORTANT: Titles should not include (or be) "Plot of...", "Graph of...", or "...over a range of data points." - Be concise, but complete. * Do not plot too many data sets on a single graph - make multiple graphs if required. WRITE A DETAILED CAPTION FOR EACH GRAPH see -- http://abacus.bates.edu/~ganderso/biology/resources/writing/HTWtablefigs.html
  • 40.