The document outlines key aspects of lab safety, including defining a laboratory, safety, and lab safety. It discusses potential hazards in labs like physical, chemical, infectious, electrical, and radiation dangers. The document emphasizes the importance of personal protective equipment, safe chemical handling, emergency procedures, and first aid for minor injuries. Following lab safety guidelines is essential to protect oneself and others from danger, risk, or injury while working in the laboratory.
2. Objectives:
Define Lab, Safety and Lab Safety.
Become aware of hazards that occurs in laboratory
Become aware of personal protection in
Laboratory
What to do and what not to do in Lab
Understand the emergency procedure, while
working in lab.
Understand the First aid procedure in case of any
accident.
3.
4. What is Lab (Laboratory)
Laboratory is a building or room
equipped for conducting scientific
research or for teaching practical
science
It is a workplace for the conduct of
scientific research
5. Safety:
Safety is the state of being certain that
adverse effects will not be caused by
some agent under defined conditions.
The condition of being safe; freedom
from danger, risk, or injury.
6. So what is lab Safety ?
Lab safety is to make certain that no any
adverse effect will be cause by any of potential
hazard or agent in the laboratory.
Lab safety procedure make sure that the
individuals in laboratory can work safely with
freedom from danger, risk or injury
7. Why does it matter?
Safe working protects:
You
Other lab workers
Cleaners
Visitors
Your work
8. What Dose Law State…
Health Safety at Work Act 1974
You must work safely
You must not endanger others
You must not misuse equipment
Penalty – up to 2 year in prison &/or an
unlimited fine
9.
10. What to do…..
Know safety symbols
• They appear in your laboratory activities
• They will alert you to possible dangers
• They will remind you to work carefully
11.
12. What to do…..
Read lab instructions ahead of time
Always follow lab procedures exactly
Ensure good general ventilation in Lab
Place all contaminated tubes and plates into
Biohazard area
Keep cultures in test tube racks when
working at lab bench or walking around the
lab.
13. What to do…..
Clean your workbench with disinfectant at the
beginning and at the end of each laboratory
exercise
Wash your hands with soap and water for at
least 1 min before and after you complete your
work.
Be Responsible at All Times
14.
15. General Hazards in Laboratory
Physical hazards
Chemical hazards
Infectious Hazards
Electrical hazards
Radiation hazards
Breakage of glassware
Sharps Objects
Spillages
16.
17. Protect Your Self….
Wear safety goggles when working with chemicals,
flames, or heating devices or if possibility of flying
debris
Keep all long hair tied back
Do not wear loose clothing that could catch on fire
Foot wear that completely covers the foot is
required
18. Protect Your Self….
• Handle glassware, sharp tools and heated
containers carefully
• Always cut away from fingers and body
• Always carry sharp objects with points and
tips facing down and away
• Never try to catch falling sharp instruments
• Grasp sharp instruments only by the
handles
19. Heat/Flame Safety
• Tie back hair and loose clothes when
working with open flames
• Never look into a container while heating it
• Never point the end of a test tube being
heated at yourself or others
• Never heat in a closed container
• Never leave a burner or hotplate
unattended.
20. Chemical Safety
Read all labels twice before removing a
chemical from the container
• Only use the type and amount of chemical
instructed to use
• Never touch, taste, or even smell a chemical
unless instructed by the teacher
• Never mix chemicals unless instructed to do
so
21. Chemical Safety….
Never mix chemicals together unless you are
told to do so (and then only in the manner
specified)
Consider all chemicals dangerous
22. Spillages
Clear up spillage promptly
Dispose of any hazardous material as toxic
waste
23.
24. You should never…..
Take any chemicals from lab or store room
Touch any equipment, chemicals, or other
materials until instructed to do so
Never use any laboratory equipment unless you
are trained & have been authorised to do so
As well as injuring yourself you may cause very
costly damage
Do an unauthorized experiment
25. You should never…..
Do not bring food or beverages into the
laboratory because you are working with
microorganisms and you do not want them to
contaminate your food or drink
Apply cosmetics
Touch your face, mouth or eyes
Practical jokes
Horse play
Rough house
26.
27. In case of an emergency….
Know the locations of:
Fire extinguisher
Fire blanket
Body shower
Eyewash station
First aid kit
Know where all the exit doors are located
28. In case of an emergency….
Know emergency procedure
If you spill a harmful chemical on yourself or in
your eyes, start rinsing immediately and send any
of your colleague to get teacher’s help
Notify instructor immediately for broken
glassware.
If culture gets into your eyes, immediately wash
your eyes for 5 minutes
31. First Aid
Injury: Cuts, bruises
What To Do: Do not touch an open wound
without safety gloves.
Pressing directly on minor
cuts will stop bleeding in a
few minutes. Apply cold
compress to bruises to
reduce swelling.
32. First Aid
Injury: Poisoning
What To Do: Find out what substance
was responsible for the
poisoning and alert the
teacher immediately.
33. First Aid
Injury: Fainting
What To Do: Provide fresh air and have the
person recline so that their
head is lower than the rest of
their body.
34. First Aid
Injury: Eyes
What To Do: Flush eyes immediately
with plenty of water for
several minutes. If a
foreign object is lodged in
the eye, do not allow the
eye to be rubbed.
35. First Aid
Injury: Electrical shock
What To Do: Shut off the current at
the source. Remove wire
with rubber gloves. Alert
the teacher immediately.