Diu Cytometriegrenoble2011
•Descargar como PPT, PDF•
1 recomendación•2,312 vistas
Monoclonal antibody, Janossy, Workshops, CDs, Cluster of dfferenciation, Mononuclear cells, lymphocytes, Immunolohy, Membrane molecules
![Où l’on cherche à identifier les leucocytes…. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)
Recomendados
Recomendados
Más contenido relacionado
La actualidad más candente
La actualidad más candente (20)
Destacado
Similar a Diu Cytometriegrenoble2011
Similar a Diu Cytometriegrenoble2011 (20)
Más de OKCC/C3O and CREM/université Lorraine
Más de OKCC/C3O and CREM/université Lorraine (20)
Diu Cytometriegrenoble2011
- 1. MC Béné Grenoble 19 Janvier 2010 GC Faure Grenoble 12/12/2011
- 6. TdT+, cALL (CD10)+ lymphoid blast crisis of Ph’+ chronic myeloid leukaemia (Lancet ii: 1058, 1976)
- 8. Dream No.1 Flow cytometry can be used for clinical leukaemia diagnosis on the ‘immunophenotyping’ platform anti-ALL (24 absorptions!) fetal development: BM is a lymphoid organ Melvyn Greaves, Geoff Brown and George Janossy
- 9. Dream No.2 from G.Janossy Monoclonal antibodies can be used for visualizing cells in tissues, normal and pathological (‘monoclonal immunohistology’ platform) CD1 (NA1/34) on human thymus: The first ever tissue section stained by the first anti-human monoclonal antibody CD1 (NA1-34) and CD45 (2D1) for T-ALL diagnosis and thymic origin Ken Bradstock, Gianni Pizzolo Bradstock KF, Janossy G, Bollum FJ, Milstein C. Anomalous phenotype in thymic acute lymphoblastic leukaemia. Nature 1980; 284(5755): 455-7.
- 10. Anticorps monoclonaux Lignée de myélome murin Déficiente en HGPRT Immunisation Rate Pas de fusion - - + Sélection des clones réactifs Clones Fusion en milieu HAT HYPOXANTHINE AMINOPTERINE THYMIDINE
- 13. La technique de Clustering Notion de distance
- 14. Et donc, les CLUSTERS de différenciation … ou CD!
- 18. Adelaide 2004 8th HLDA : 339/350 CD Barcelone 2008 363
- 25. ORGANISATION DES FEUILLETS BETA-PLISSES Les feuillets clairs sont sur le dessus (cercle clair), les feuillets foncés en-dessous (cercle foncé) Ils sont reliés entre eux par des boucles dont certaines peuvent porter des CDR PONT DISULFURE Représentation schématique de face Représentation schématique de profil
- 32. CD95L CD178 CD27L CD70 CD30L CD153 CD40L CD154 TRAILCD253 OX40L CD252 RANKLCD254 APRIL CD256 BAFF CD257 TNFR1 CD12a TNFR2 CD12b
- 35. Récepteurs aux cytokines à CD132
- 36. Récepteurs aux cytokines à CD130 et CD131
- 37. Récepteurs aux cytokines de classe 2
- 42. CD183 CXCR3 CD184 CXCR4 CD194 CCR4 CD195 CCR5 CD197 CCR7 v
- 43. Molécules d’adhésion © Pr MC Béné CD34 Sialomucines CD31 Superfamille des Ig CD62E Sélectines CD49a Intégrines CD29 Tetraspanines CD9
- 47. Beta 1 = CD29 Alpha 1 à 6 = VLA 1 à 7 = CD49 a à g Beta 2 = CD18 Alpha L = CD11a Alpha M = CD11b Alpha X = CD11c Alpha E = CD103… RGD receptors = Tripeptide Arg-Gly-Asp
- 61. Marqueurs associés à la lignée B CD19 ITAM CD22 or ITIM ITIM ITIM ITIM ITAM CD20 P CD24 CD21 BCR NH2 COOH CD23
- 62. CD1 CD2 TCR CD3 ITAM ITAM ITAM ITAM ITAM ITAM ITAM ITAM ITAM ITAM CD4 CD5 CD8 CD7
- 63. Marqueurs associés aux cellules myéloïdes CD13 E NH2 COOH E CD14 L L L L L L L L L L P CD33 CD11b Mg Mg Mg MPO K CD117 CD15 CD65 3-fucosyl-N-acetyl-lactosamine céramide dodecasaccharide CD36 CD35 CD16
- 64. Integrated collaboration Requires strong leadership and a long time-scale (10-15 yrs) Before a new drug But so many underway
- 65. http://1degreebio.org/ De la NLM S Shaw PROW … 339 http://www.sciencegateway.org/resources/prow/index.html à Via l’ASSIM … 363 http://www.thefcn.com/cd_list_hlda_9_barcelona_2010.html
Notas del editor
- The membrane microdomains include lipid rafts, tetraspanin-enriched microdomains (TEM) and podosomes or invadopodia. The association on the tumour cell membrane of cathepsin B with caveolae, a subset of lipid rafts that contain the structural protein caveolin, is mediated by a direct interaction of procathepsin B with the light chain of the annexin II heterotetramer (AIIT). Membrane microdomains are dynamic with constituents also present in intracellular vesicles such as multivesicular bodies, MHC (major histocompatibility complex) class II compartments (MIIC) and exosomes that also contain cysteine cathepsins such as (pro) cathepsin L and cathepsin S. These associations might enhance secretion of cysteine cathepsins, including at podosomes or invadopodia, at which sites CD63 co-localizes with p61Hck, a 'lysosome-associated' Src family kinase. The urokinase plasminogen activator receptor in association with 1-integrin is found in many of the same membrane microdomains, perhaps indicative of an association between these two proteolytic pathways at this sites.
- http://1degreebio.org/