9. BioBrick® standard parts
EcoRI XbaI BioBrick part SpeI PstI
Knight, 2003
10. BioBrick® standard assembly
E X BioBrick part 1 S P E X BioBrick part 2 S P
Digest with Digest with
EcoRI and SpeI XbaI and PstI
E X BioBrick part 1 S E X BioBrick part 2 S P
Ligate
E X BioBrick part 1 Mixed BioBrick part 2 S P
32. Measurement kit
E X E0240 S P
E. coli strain
GFP reporter
TOP10
pUC AmpR
E X P1010 S P E X E0240 S P
Medium copy Promoter reference
BioBrick plasmid standard
p15A KanR p15A KanR
38. Lessons
• Ad hoc reference standards are useful
• Reference standards materials and
instructions should be distributed
• Standards enable comparison of parts
• Standards help to identify sources of
variability for part improvement
42. System is coupled to the
chassis
a
Ribosomes
RNA Polymerases
Chassis gene Engineered System
b
Ribosomes O-ribosomes
43. Decouple system and chassis
RNA Polymerases
Chassis gene Engineered System
b
Ribosomes O-ribosomes
T7 RNAP
RNA Polymerases
Chassis gene Engineered System