Science Fair Presentation

Mixed Monolayers on Silicon for DNA Attachment Goal ,[object Object],[object Object]

Properties of DNA ,[object Object],[object Object],[object Object],[object Object],Right: Image location URL is http://www.biologycorner.com/bio1/DNA.html Left: Image Source Not Found

The AFM ,[object Object],[object Object],[object Object],Left: Image Source Not Found Right: Image Source is http://www.farmfak.uu.se/farm/farmfyskem-web/instrumentation/afm.shtml

Mica and Buffer ,[object Object],[object Object],AFM image of DNA on mica with buffer AFM images by Alexander Lykoudis Diagram Drawn By Dr. Koshala Sarveswaran Mica (anionic) Mg Mg Mg Mg Mg Mg Mg Mg Mg Mg Mg DNA (anionic) Mica (anionic) Mg Mg Mg Mg Mg Mg Mg Mg Mg Mg Mg

Why DNA on Silicon? ,[object Object],[object Object],[object Object],[object Object],Image from www.toyo-adtec.co.jp

How It Happened ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Why Mixed Monolayers? ,[object Object],[object Object],[object Object],[object Object],Image Drawn By Dr. Koshala Sarveswaran Si Silicon APTES DNA

Background of Experiment N-Trimethoxysilylpropyl-N,N,N-trimethylammonium chloride This is mixed with APTES in various proportions to see how the DNA reacts… N EtO Si OEt EtO H H APTES Structures Drawn By Dr. Koshala Sarveswaran OMe N Si OMe MeO Me Me Me

Experiments ,[object Object],[object Object],[object Object],[object Object],DNA

Experiment 2 ,[object Object],[object Object],Yellow circular shapes are DNA with the pink being DNA clumped on one another

Experiment 3 ,[object Object],[object Object]

Experiment 4 ,[object Object],[object Object],Without DNA With DNA

Experiment 6 ,[object Object],[object Object],Without DNA With DNA DNA

Experiment 7 ,[object Object],[object Object],Without DNA With DNA

Discussion and Conclusion ,[object Object],[object Object],[object Object]

Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Bibliography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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