1. ISSN (Print): 2328-3777, ISSN (Online): 2328-3785, ISSN (CD-ROM): 2328-3793
American International Journal of
Research in Formal, Applied
& Natural Sciences
AIJRFANS 14-227; © 2014, AIJRFANS All Rights Reserved Page 51
Available online at http://www.iasir.net
AIJRFANS is a refereed, indexed, peer-reviewed, multidisciplinary and open access journal published by
International Association of Scientific Innovation and Research (IASIR), USA
(An Association Unifying the Sciences, Engineering, and Applied Research)
Antibacterial Investigations & Spectral Characterization of the Complex of
Se4N3Br with Co (II) Compound
Govind Kumar Gupta & S.P.S. Jadon
Department of Chemistry, S.V. College, Aligarh (U.P.) 202001-India
I. Introduction
The complexes of Se4N4 & its chloro derivatives have been reported2-8
. The adducts of Urea and thiourea with
Se4N3Cl have also been synthesized and investigated9,10
. The metal complexes such as Mn(II), Fe(III), Co(II) &
Ni(II) with Se4N3Br prepared, have been reported11-13
.
II. Experimental
During the synthesis of Se4N4
14
and Se4N3Br (loc.cit.) Anal R. Grade doubly distilled chemicals were used. To
prepare the complex of Se4N3Br with Co(II), Se4N3Br (0.5gm) and CoCl2 (0.5gm), in 1:1 ratio was mixed using
DMF as solvent and refluxed for 6-8 hrs on a hot plate at the temperature of 150-1600
C. A brownish black mass,
formed, was separated after washing with DMF, alcohol and ether, and dried at 1100
C.
Its U.V. spectrum was recorded on Perkin-Elmer-Lambda-15 spectrophotometer in the range of 200-800 nm,
while E.P.R. and X.R.D. spectra were graphed on Varian’s X-E-4 bands spectrometer and PW-1710
Diffractometer using Cu as a source of radiation ( = 1.5418 Å ) at room temperature. The complex was
treated against S. aureus (gram +ve) and E.coli (gram ve) bacteria by using invitro technique and making 5
mg/ml test solution to check the inhibition of bacteria in presence of complex.
III. Results and Discussion
U.V. spectrum (Fig-1) consist five peaks. Out of which former band at 200nm having the absorbity 0.788
equivalent to 6.2ev energy is on account of charge transfer transition in the complex. The other band at 282.5
nm is due to transition of Se4N3 ring in the complex.
Fig. 1: U.V. spectrum of the Complex
This view is upheld by the value of oscillator strength ‘f’ = 0.4866x10-5
(Table-1,Column-3) for the spin
allowed Laporte forbidden transition which is caused by the sharing of electrons i.e. covalent bonding. The
remaining of three assignments at 570.0nm (17543.85 ), 690.0nm (14492.75 ) and 777.5nm
(12861.73 ) are consequently for the following transitions15
:
4
T1g(P)  4
T1g(F)
4
A2g(F)  4
T1g(F)
Abstract: On the basis of Mass and I.R. Spectra, the complex of Co(II) with Se4N3Br formulated1
as
(Se4N3)4CoCl2, analyzed by recording its U.V., E.P.R. and X.R.D. Spectra. The results have revealed that the
complex is conductive and paramagnetic in character with 3d5
configuration, octahedral array and
tetragonal packing of the molecules. The complex is 14 mm and 10 mm effective against S. aureus (gram +ve)
and E. coli (gram ve) bacteria respectively.
Keyword: Magnetic Susceptibility, Se4N3Br, Geometry, Tetragonal.

2. Govind Kumar Gupta et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014,
pp. 51-54
AIJRFANS 14-227; © 2014, AIJRFANS All Rights Reserved Page 52
4
T2g(F)  4
T1g(F)
Suggesting the hexadentated coordinated bonding along with octahedral structure of the complex with 3d7
configuration. Low value of band gape energy, Eg and high value of number of conducting electron, Nc (Table-
1, Column-4&5) expounds the good conductivity of complex.
The E.P.R. spectrum (fig.2) with hyperfine appearance possess many prominent peaks of low intensity. Out of
which some peaks have been selected for the interpretation. For these peaks at different magnetic field the value
gx, gy found, are less than two (Table-1 Column-7) indicating the presence of vacant ‘d’ energy shells to accepts
electron pair from N-atom of Se4N3 ring to form coordinate linkage which is supported by the narrowness of the
peaks caused by exchange of electron pairs.
The value of gz more than two (free electrons),( Table-1 Column-8) infers the covalent linkage in the complex
due to Se4N3 ring in the complex. The paramagnetism of the complex is supported by the low value of magnetic
moment, and magnetic susceptibility (Table-1 Column-9&10). The values of No. of unpaired electrons,
calculated from the values of , is found one, which is for the 3d7
configuration of Co2+
. During the reaction
of CoCl2 with Se4N3Br, one electron 3d7
has been transferred to Se4N3 ring.
Fig. 2: E.P.R. spectrum of the Complex
Table-1: U.V. and E.P.R. Spectral Data of the Complex
U.V. Spectral Data E.P.R. Spectral Data
Band
Assigned
nm (cm1
)
Transition f10-5 Eg
(ev)
Me 
105
Magnetic
Field H
(Gauss)
gx = gy gz
eff
(BM)
A 
103
(e.s.u.)
1 2 3 4 5 6 7 8 9 10
200.0
(50,000)
C.T. 2.33256 - - 1837.614 0.3522 3.6794 1.8565 1.4365
282.5
(35398.23)
p  d 0.48659 0.9055 2.8304 1937.614 0.5407 3.4895 1.7861 1.3297
570.0
(17543.85)
4
T1g(P)4
T1g(F) 0.09744 1.1070 1.7401 1994.757 0.6399 3.3896 1.7541 1.2824
690.0
(14492.75)
4
A2g(F)4
T1g(F) 0.09144 0.1890 2.6270 2189.995 0.9399 3.0874 1.6807 1.1773
777.5
(12861.73)
4
T2g(F)4
T1g(F) 0.12791 0.1010 21.9924 2309.042 1.0979 2.9282 1.6572 1.1446
2504.280 1.3245 2.6999 1.6430 1.1251
2542.376 1.3647 2.6595 1.6430 1.1251
2809.042 1.6153 2.4070 1.6592 1.1474
2889.995 1.6823 2.3396 1.6683 1.1601
3156.661 1.8786 2.1419 1.7063 1.2133
From the X.R.D. (fig. 3), graphed, in 2 range (00
-800
) the value of sin2
, miller indices, hkl and inter planar
distance ‘d’ (Table-2) are determined. The values of ‘d’ resembles to theoretical ones. The axial distances a0 =
7.8427 Å, b0 = 7.8427 Å and c0 = 13.5839 Å axial angels === 900
are corresponding to a0 = b0  c0 and  = 
=  = 900
for tetragonal array of the complex confirming the given (loc. cit.) structure. (fig-5).

3. Govind Kumar Gupta et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014,
pp. 51-54
AIJRFANS 14-227; © 2014, AIJRFANS All Rights Reserved Page 53
 Angle 2 (o
) 
Fig. 3: X.R.D. Pattern of the Complex
Table-2: X-ray Diffraction pattern of complex G-3
S. No.
2(o
) Sin2
 (h2
+ k2
+ l2
)Qs hkl d(Ao
) Obs (theo) dhkl (Å)
1 2 3 4 5 6
1 11.08 0.00932 1 (0.00932) 100 7.9853 (7.9785) 7.8417
2 16.96 0.02174 2 (0.01087) 110 5.2278 (5.2233) 5.5449
3 23.03 0.03984 4 (0.00996) 200 3.8618 (3.8568) 3.9208
4 29.17 0.06341 6 (0.01057) 211 3.0614 (3.0578) 3.2013
5 32.15 0.07667 8 (0.00958) 220 2.7841 (2.7809) 2.7724
6 40.77 0.12133 13 (0.00933) 320 2.2132 (2.2108) 2,1748
7 43.03 0.13450 14 (0.00960) 321 2.1020 (2.0998) 2.0958
8 51.20 0.18669 20 (0.00933) 420 1.7842 (1.7826) 1.7534
9 55.37 0.21586 24 (0.00899) 422 1.6592 (1.6576) 1.6006
10 61.03 0.25782 27 (0.00954) 511 1.5182 (1.5167) 1.5091
11 64.41 0.28403 30 (0.00947) 521 1.4465(1.4451) 1.4317
12 67.62 0.30962 33 (0.00938) 522 1.3854 (1.3842) 1.3650
13 71.16 0.33853 35 (0.00967) 531 1.3249 (1.3238) 1.3254
Qav = 0.009662
a0 = 7.8427Å, b0 = 7.8427 Å, c0 = 13.5839 Å and = = = 90
The complex was treated against the S. aureus (gram +ve) & E. coli (gram ve) bacteria (Fig. 4) by using
invitro technique and found 14 mm and 10 mm inhibition respectively. The screening suggest that the complex
may be used as medicine for the disease such as diarrhea, anemia, skin disease, pneumonia etc. caused by these
bacteria.
S. Aureus (gm +ve) bacteria effect of complex E. Coli (gm ve) bacteria effect of complex
G-3 – Co(II) G-3 – Co(II)
Fig. 4: Zone inhibition of complexes against S. aureus and E. coli

4. Govind Kumar Gupta et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014,
pp. 51-54
AIJRFANS 14-227; © 2014, AIJRFANS All Rights Reserved Page 54
IV. Conclusions
The complex, (Se4N3)4CoCl2 is a good conductor having paramagnetic character along with octahedral structure
with tetragonal packing of molecule. The complex is also found effective against S. aureus (gram +ve) & E. coli
(gram ve) bacteria.
Acknowledgement
The authors are thankful to the Directors, SAIF Punjab University, Chandigarh, SAIF, IIT Bombay and ACMS
IIT Kanpur to provide the Instrumental facilities.
Co2+
N3Se4
Se4N3
2Cl
-
N3Se4
Se4N3
Fig. 5: Structure of Complex (Se4N3)4CoCl2
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