This lab document provides instructions for identifying macromolecules like carbohydrates, proteins, and fats in various foods using indicator solutions. Students will test food samples like glucose, starch, and lipids using Benedict's solution to detect simple carbohydrates, IKI solution for complex carbohydrates like starch, Biuret solution for proteins, and Sudan IV for lipids. Each indicator solution is described along with the color change expected for a positive result identifying the macromolecule present. Step-by-step procedures are outlined for setting up and conducting each macromolecule test.

1. IDENTIFYING MACROMOLECULES IN FOOD LAB Adapted from: Madison Southern High School Biology

6. Background Information reddish - orange dark red lipid Sudan IV violet , black blue protein Biuret solution black dark red complex carbohydrate IKI solution orange blue simple carbohydrate Benedict’s solution POSITIVE TEST NEGATIVE TEST MACRO-MOLECULE INDICATOR

21. LAB SAFETY and CLEAN UP WEAR safety goggles and apron at all times THOROUGHLY CLEAN lab area and equipment NO EDIBLE products in lab