SlideShare una empresa de Scribd logo
1 de 57
STERILIZATION & DISINFECTION OF
ORTHODONTIC INSTRUMENTS AND
CONSUMABLES

INDIAN DENTAL ACADEMY
Leader in continuing dental education
www.indiandentalacademy.com
CONTENTS

 INTRODUCTION
 STERILIZATION AND DISINFECTION- DEFINITION
 INACTIVATION OF MICROORGANISMS
 INFECTION CONTROL
• BARRIER PROTECTION
• ENVIRONMENTAL SURFACE DISINFECTION
 CONTAMINATION VEHICLES
 STERILIZATION AND ITS METHODS
 DISINFECTION AND ITS METHODS
 EFFECTS OF STERILIZATION & DISINFECTION ON
ORTHODONTIC MATERIALS
Effects on orthodontic wires
Effects on orthodontic pliers
INTRODUCTION
For protection of both the doctor and patient, sterilization
techniques are of utmost importance in preventing the
spread of infectious disease. This is of special significance
in dentistry because more microorganisms are found in the
oral cavity than in any other part of the body. With the
increasing number of adult patients and diverse life-styles,
the orthodontist is more at risk than ever to exposure to
serious pathogens and must take precautions to guard
against their transfer.
Orthodontists have the second highest incidence of
hepatitis B among dental professionals (Starnbach, 1980).
Saliva is about half as infectious as blood, and the most
likely modes of transmission in dental offices are through
puncture wounds, skin abrasions, or lesions.
Dental aerosols, splattering, and instrument contamination
can also transmit the virus, which can survive for several
weeks at room temperature.
Hepatitis B (serum hepatitis), herpes, and AIDS viruses
are certainly the more serious diseases of many that can be
contracted in an orthodontic office. The HTLV-III (AIDS)
virus is more fragile and less infective; transmission is
most likely to occur after repeated blood-to-blood or
blood-to-mucosa contact or sharing of hypodermic
needles. Still, the increasing incidence of AIDS, as well as
of hepatitis B, has made orthodontists even more aware of
the necessity of decontaminating instruments and surfaces.
STERILIZATION: Sterilization is a process by
which articles are freed of all microorganisms both
in vegetative or spore state.
DISINFECTION: Disinfection is a process of
destruction of pathogenic organisms capable of
giving rise to infection.
ANTISEPTIC: It means prevention of infection by
inhibiting growth of bacteria.
INACTIVATION OF MICROORGANISMS
Hepatitis virus→ Kobayashi and associates (1984),
using direct chimpanzee inoculation as an assay method,
found that 0.1% aqueous glutaraldehyde at 24° C for 5
minutes, 1% aqueous glutaraldehyde at 24° C for 1
minute, 80% ethanol at 11° C for 2 minutes, and heat at
98° C for 2 minutes are all able to inactivate hepatitis B
virus in human plasma.
AIDS virus→ AIDS virus is inactivated after treatment with
50% ethyl alcohol, 35% isopropyl alcohol, 0.1% household
bleach, Lysol, and 0.3% H2O2 at room temperature (21° to
25° C) for 2 to 10 minutes. Heating at 56° C for 30 minutes
inactivates the AIDS virus, and one investigator found the
virus titer level dropped to undetectable levels after 10
minutes of heating at 56° C in the presence of serum.
HTLV III/LAV has unusual stability at room temperature.
Infective viral particles have survived for more than a week at
20° to 22° C in either dry form or liquid medium.
INFECTION CONTROL
An infection-control program comprises two distinct areas,
1) Exposure control and
2) Hazard communication
Exposure control covers sterilization and disinfection, waste
management, and employee safety and education, including
personal protective equipment and bodily-fluid-exposure
protocols.
Hazard communication requirements include drills for hazard
communication plans (chemical spills, emergency first aid,
and fire or tornado evacuation), secondary labeling of
hazardous chemicals, Material Safety Data Sheets, x-ray
updates, and properly displayed state and federal posters.
A chair side assistant or records technician should be
appointed as the office Environmental Safety
Coordinator. The Environmental Safety Coordinator’s
responsibilities include:
• Keeping all manuals and secondary labeling current
• Conducting staff training annually, or as procedures
change
• Keeping records of annual hepatitis-B vaccinations for
patient-contact employees
• Conducting and documenting weekly spore tests or other
appropriate monitoring of sterilization equipment
• Maintaining infection-control inventory
• Cleaning equipment nightly, weekly, or monthly as
required
• Filing exposure-incident reports
The main tasks of infection control are implementation of
barriers to the spread of pathogens, disinfection of
environmental surfaces, and sterilization
Barrier Protection
Long-sleeved, jewel-neck clinic
jackets should be worn in the
laboratory and operatory. These
jackets should be replaced daily,
or when visibly soiled, and worn
only in the office.
Masks and protective eyewear are
required during bonding and
debonding procedures to protect
against aerosols of blood and saliva.
Face shields or side shields should be
added to personal eyeglasses. Masks
and face shields are required
whenever a handpiece is used.
Patients should be provided
eyewear during any procedure
with a risk of eye injury from
debris or chemical agents.

Latex gloves must be worn for all patient procedures, and
changed between patients. Gloves must also be removed
and replaced before handling materials such as charts,
study casts, and radiographs.
Environmental Surface Disinfection
In the operatory, use a spray-wipespray technique with a phenol solution
that cleans, disinfects, and deodorizes.
After each patient, any exposed
surfaces—chairside units, pencils, pens,
counters, chairs—are sprayed with
phenol and wiped down with a paper
towel. This reduces the number of
microbes that must be killed and
removes any bodily fluids that may
insulate the remaining microbes.
The surfaces are then resprayed and allowed to dry for 10 minutes,
after which excess moisture is wiped away. Dental chairs only
have to be sprayed after handpiece procedures that can create
aerosols.
Clear plastic wrap should be used over the handpiece
consoles and over all chairside switches or handles.
In the laboratory, alginate impressions are rinsed with
water after removal from the patient’s mouth, sprayed with
Biocide, and placed in reclosable sandwich bags. (Phenols
such as Birex should not be used because they can distort
impressions).
The impressions are rinsed again with water and dried
before pouring, and they are always handled with latex
gloves. Finished appliances are placed in sandwich bags or
retainer boxes until delivery.
CONTAMINATION VEHICLES
Payne in AJO 1986 explained the three possible pathways
of cross-contamination and its handling methods as
1) Critical— Instruments that penetrate the mucosa
must be sterilized.
2) Semicritical— Instruments that touch the mucosa
should be sterilized.
3) Least critical— Surfaces touched during treatment
should be disinfected.
Using this as a guide, scalers, scalpels, and other tissuecutting instruments must be sterilized. Other hand
instruments and pliers should be sterilized. Work
surfaces, triplex syringe handles, operating light handles,
and other environmental surfaces should be disinfected.
Shanon T. Kirchhof in JCO 1987 pointed out that to
prevent the spread of infection in an orthodontic office,
there are three main contamination vehicles that must be
effectively sterilized,
1) Instruments contaminated with blood or saliva
2) Surfaces contaminated with blood or saliva, and
3) Staff members' hands
Instruments
Instruments requiring sterilization include mirrors,
pliers, scalers, banding and bonding instruments, bands,
impression trays, cotton pliers, and ligature directors.
Some, including plastic band seating instruments, cheek
retractors, mirrors, and bite planes, cannot withstand
heat sterilization.
If ethylene oxide is not being used and the instrument
would be damaged by heat sterilization, it should be
thoroughly debrided and submerged in a fresh solution of
glutaraldehyde or formaldehyde for one to 10 hours. The
length of time depends on the sterilant used and the
temperature of the solution.
Surfaces
Surfaces that cannot be sterilized should be effectively
disinfected. These surfaces include bracket trays,
air/water syringes, saliva ejector handles, chair control
buttons, operatory light handles, sink handles, supply
drawers, and chair armrests and headrests. Each office can
prepare its own list of operatory surfaces.
Proper office and equipment design will reduce the number
of surfaces that need disinfecting. Foot-activated chair
controls and foot- or knee-operated sinks and soap dispensers
are a few ways to avoid hand contact.
Contaminated surfaces can be scrubbed with iodophorsoaked 4" × 4" gauze pads and allowed to dry. Gauze soaked
with 70 percent alcohol can be used to remove the residue
after drying. The iodine solution has a built-in antimicrobial
activity indicator— when it turns from amber to clear, it
should be replaced. Iodophors have a slight allergenic
reaction with skin and can stain light-colored surfaces after
repeated use.
The recently introduced Vitawipes are disposable disinfectant
cloths containing polyhydrochloride (3.2 percent), alkyl
dimethyl, benzyl ammonium chloride (7.1 percent), and inert
ingredients (89.7 percent). A cloth is dampened with water
and used to wipe contaminated surfaces. It does not kill
bacterial spores, mycobacterium tuberculosis, or hepatitis B
virus on contact, but disinfects by physically removing the
potential pathogens. The cloth is disposed of after an
indicator strip changes color or after wiping a surface
contaminated with hepatitis B virus. The wipes are easy to
use and show minimal reaction with skin, but they have not
been fully tested.
Hands
Hands are the most common link
in the contamination chain.
Proper handwashing reduces the
number of potential pathogens,
but it does not sterilize the skin.
The hands, fingers, and
fingernail bed have breaks in the
epidermis that cannot be seen
with the naked eye.
Patients' blood has been retained under the fingernails of
unprotected hands for five or more days after patient contact.
Hands should be washed for 20 seconds with an effective
antimicrobial soap. The soap should have residual action
because of the warm, moist environment created by gloves.
Gloves provide the necessary
physical barrier from pathogenic
organisms and should be worn
while treating all patients. The
smooth surface of gloves also
allows more effective handwashing
between patients.
Gloves must fit properly to prevent loss of dexterity. Hand
cream can be applied before glove use if one is sensitive to talc
or cornstarch.
Masks and eye protection are recommended to protect from
aerosols and particle debris produced by handpieces, air/water
syringes, trimming and polishing burs, and other equipment.
Fernando Ascencio in JCO 1998 studied whether
orthodontic marking pencils can pick up and transfer
bacteria from patient to patient during typical orthodontic
procedures. The results showed that marking pencils can
transfer bacteria from contaminated archwires.
He concluded that conventional orthodontic marking
pencils cannot be autoclaved. Gas sterilization is
effective in killing bacteria, but is also costly and
difficult, making it impractical for orthodontic offices.
Soaking or spraying the tips of marking pencils
with disinfectants could be more effective than
wiping, but this method is unlikely to gain
acceptance from practitioners. The only sure way
to avoid potential cross -contamination is to use the
inexpensive disposable markers available from
orthodontic supply companies.

Metal and elastomeric ligatures are potential agents in the
transmission of infectious diseases. Mulick (1986)
recommended single-use dispensing of elastomeric materials
to eliminate contact of canes or sticks with contaminated
hands.
Schneeweiss in JCO 1993
described a method of cutting
elastomeric modules into smaller
sections and covering them with
clear tubing, which could then be
cold sterilized.
During archwire placement, the
operator contacts only the outside
tubing while removing ligatures
More recently, dispensers have been introduced onto the
market, but the effectiveness of such dispensers in
controlling cross -infection has yet to be fully evaluated.
Takla in JCO 1998 evaluated the
effectiveness of a new elastomeric
module dispenser in reducing bacterial
contamination, as compared with the
existing method of storing and
dispensing elastomeric modules on
canes.
He concluded that the Alastik
elastomeric module dispenser proved
to be efficient in limiting crossinfection through single-use
dispensing, although it did not offer
protection against handling and
environmental factors, such as dust.

Alastik elastomeric
module dispenser
STERILIZATION AND ITS METHODS
Sterilization destroys all microorganisms, including
viruses and spore forms, and usually involves the use of
heat. To sterilize properly and avoid damaging
instruments, specific steps must be followed.
After each patient procedure, sharps are discarded in a
sharps container, and disposable items in a recessed,
plastic-lined wastebasket. Plastic items that cannot be
autoclaved are placed overnight in Procide, an immersion
sterilization solution. Heat-sterilizable cheek retractors are
also immersed in Procide, since they tend to turn milky
after autoclaving. Handpieces and photographic mirrors
are sterilized in a Kavo-Klave.
Rinsing reduces the amount of contaminating solids before
the instruments are debrided with an ultrasonic cleaner. If
infected material is not removed, the time required to
destroy all microorganisms may be increased. Heavy gloves
should be worn to protect personnel from possible
contamination.
Depending on the sterilization method, the instruments are
then placed on a rack or towel or are wrapped.
Plier racks and instruments are placed
in a wire basket and run through an
ultrasonic cleaner containing a rustinhibiting Non-Ionic Multipurpose
Ultra-sonic Cleaner. Ultrasonic
solutions should be changed daily and
covered during cleaning to reduce
aerosols. Instruments and pliers should
not be rinsed after ultrasonic cleaning.
Any dried blood that remains must be
scrubbed off by hand, and the
ultrasonic cleaning must then be
repeated before sterilization.
Alternatively instruments are dipped in a Sodium Nitrite
Rust Inhibitor, drained of excess sodium nitrite, and made
ready for sterilization.
After ultrasonic cleaning, the next step is to thoroughly dry
the instruments. Hinged instruments may be "milked" with
a water-soluble oil dip to lubricate them and prevent
corrosion.
The sterilization methods require different combinations of
time and temperature.
Sterilization can be accomplished in one of several ways.
Some of the most common ways that are followed in
orthodontic practice include,
1) Steam autoclave sterilization
2) Dry heat sterilization
3) Glass bead sterilization
4) Chemical vapour sterilization
5) Ethylene oxide sterilization
Steam autoclave sterilization
Steam sterilization (autoclave)
uses saturated water vapor at
240° F, with 15 pounds of
pressure for 15 to 40 minutes.
The time can be reduced to three
minutes by raising the pressure to
30 psi and the temperature to
270° F. More time is required for
heavily wrapped loads of
instruments.
Sterilization can be verified with indicators and spore tests. It
is a time-tested method that has little value for orthodontists
because it severely rusts pliers and damages cutting edges.
The corrosion may be reduced by dipping the instruments in a
milk-like emulsion of oil in water prior to sterilization.
Dry heat sterilization
Dry heat provides a relatively low-cost
sterilization procedure. Dry heat ovens
require one hour at 320-340°F for
sterilization. Wrapping or increasing
the number of instruments increases the
time required. It has two major
drawbacks. It requires from 1 to 2 hours
at 320° F for a complete cycle— far too
long being practical for inventory
considerations. A lesser problem is the
tendency for the air to stratify and cause
uneven temperatures that result in a
lack of sterility.
Glass bead sterilization
Heat transfer media (salt or glass bead sterilizers) have
been shown effective against most organisms and spores.
There is some evidence that reliable, broad-spectrum
sterilization occurs only with small instruments. Bulky
instruments are not recommended because they may
cool the medium below the reliable temperature for
sterilization.
Glass bead sterilization uses small
glass beads ranging from 1.2 to 1.5
mm in diameter. The suggested
heating range is 424° to 450° F31
(217° to 232° C) for 3 to 5 seconds
but not exceeding 482° F (250° C).
A relationship exists between the
size and working surface of an
instrument and the temperatures
attained in the bead sterilizer. The
larger the instrument, the longer the
heat-up time required.
A narrow, deep well is preferable
to a wide, shallow one; instruments
should be placed deep and near the
sides of the wall for best results.
Gerald E. Smith in AJO 1986 determined the capability of
glass bead sterilization in sterilizing orthodontic bands and
compared bead sterilization to other methods of cleansing
and disinfecting orthodontic bands used in the office setting.
The bands were placed one at a time into a 226° C bead
sterilizer for 15, 30, 45, and 60 seconds.
The results indicated that 15 seconds is required to sterilize
bacteria and 45 seconds required for spores.
A longer bead sterilization time is required if more than one
band at a time is placed in the well. Twice the amount of
time or 90 seconds is needed if five bands are inserted
simultaneously due to the limited size of the well and
fluctuation in temperatures with depth.
Other methods of disinfecting orthodontic bands,
including tap water rinse, soap scrub, 30-minute
alcohol soak, and alcohol flame, are not adequate
to prevent growth of Staphylococcus albus and
Bacillus subtilis cultures with one exception—
alcohol flame appears capable of preventing
growth on bands inoculated with bacteria.
Chemical vapour sterilization
Unsaturated
chemical
vapor
sterilization
(Chemi-clave)
is
a
suitable
method
for
orthodontic instruments.
Chemical vapor sterilizers
use
formaldehyde,
alcohols, and water. The
clean, dry, unwrapped
instruments are set on a
tray in the chamber, and
the unit is set at 270° F at
20-40 psi for 20 minutes.
When the chamber is opened, the toxic formaldehyde
vapor must be vented to the outside.
Because an unsaturated vapor is used, rusting is not a
problem. It has a cycling time that is practical for an
orthodontic office. Its chief drawback is a chemical odor
that, although not harmful, requires adequate ventilation.
Ethylene oxide sterilization
Ethylene oxide is useful in as much as towels, and metal
and plastic instruments may be sterilized simultaneously.
It is the only major sterilization technique that does not
require heat above room temperature. Hyperbaric gas
(ethylene oxide) sterilization is recommended for
instruments that are prone to corrosion or heat damage.
However, the process is slow and costly, and the effluent
gas is highly toxic.
Standard treatment varies with temperature: 12 hours are
required at room temperature, four hours at 56°C. It has
the disadvantages of being toxic, allergenic, requiring a
long exposure time, and is explosive if mixed with air. It
is therefore combined with an inert gas such as carbon
dioxide to render it nonexplosive. Another disadvantage
is that materials retain varying amounts of ethylene
oxide gas after removal from the sterilizer, and this must
be allowed to dissipate before use.
Glutaraldehydes— alkaline, acidic, and heat-potentiated—
are effective sterilants for instruments other than pliers, but
only when used for 6 to 10 hours. Again, this is an
impractical cycle time. Their best use is for plastics and
other heat-sensitive items.
After heat sterilization, each rack of sterilized instruments
is then kept in a cool-down drawer, which is lined with
plastic laminate to control moisture, until the instruments
and pliers can be returned to storage.
It is important that cutting instruments be rotated for
periodic resharpening. Plier hinges can be lubricated as
necessary.
The most common inefficiencies in orthodontic sterilization
procedures are overhandling of instruments and improper
chairside clean-up. Contributing factors can include
mislocation of the sterilization area, poor flow control of
breakdown and sterilization, excess instrumentation, and
poor storage organization.
Many orthodontists practice overkill procedures that are not
required by any regulations, such as bagging individual
instruments, wearing masks for all procedures, spraying
chairs after every patient, maintaining an in-house laundry,
and buying several different products when one will do the
job.
DISINFECTION AND ITS METHODS
A number of methods have been used in orthodontic
offices to disinfect instruments and environmental surfaces. A
70% alcohol solution has been the most widely used even though
the least effective. A 1% solution of sodium hypochlorite
(bleach) is very effective, but hard on the skin and has an
unpleasant odor. The iodophors are the best choice. They are
inexpensive, have residual effectiveness, and are easy to use and
store.
Their single drawback is the light brown residue left on surfaces,
which disappears as the compound oxidizes. It does not stain as
iodine does. These solutions can be made by diluting 1 oz
povidone-iodine preparation in 16 oz of 70% isopropyl alcohol.
They are also available in dry form to be diluted with water.
Quaternary ammonium compounds (QAC)
A quaternary ammonium compound (QAC or "quat")
reduces the surface tension between bacteria and an
object, thus disrupting the bacterial cell wall.
Concentration, degree of contamination, level and
extent of contact, and presence of other compounds all
play a role in QAC effectiveness. Cotton, air, gross soil,
or unusually heavy bacteria can prevent contact of the
disinfectant with the cell wall. Combining several
disinfectants— for example, a QAC with a phenolic
compound containing an anionic detergent— can cause
them to neutralize each other.
Quaternary ammonium compounds (QAC) are used
routinely for hand instruments because the metal
remains bright and shows no sign of corrosion. They
have a pleasant odor and a short time cycle.
Disadvantages of QAC include, their inactivation by
soap, reduced effectiveness in the presence of organic
matter, incompatibility with many chemicals found in
dental offices, and limited effectiveness against gramnegative organisms, spores, and viruses.
Phenol
Phenol is not itself used as a disinfectant, but many
disinfectants have been derived from it. At high
concentrations, phenol is a rapid protoplasmic poison
that penetrates the cell wall and precipitates the cell
protein. The effectiveness of phenolic compounds
depends on contact with the bacterial cell. These
compounds are effective against vegetative bacteria,
lipophilic viruses, and tuberculosis, but not against
bacterial spores or hydrophilic viruses.
Alcohol
Alcohol is a moderate disinfectant that behaves similarly
to a QAC. Absolute alcohol is less effective than a 70
percent aqueous solution. Isopropyl alcohol is more
effective than ethyl alcohol, but neither is effective
against spores. Alcohol is generally bacteriocidal against
vegetative forms. However, the American Dental
Association (ADA) does not recommend alcohols, QACs,
or phenolic compounds for use in dentistry, because they
are nonsporicidal and ineffective against hepatitis B virus.
Chlorine
Chlorine in aqueous solutions, even in minute amounts, is
rapidly bacteriocidal. The exact mechanism of this activity is
not known, but theories range from cell wall damage and
enzyme system blockage to protoplasmic poisoning. Chlorine
disinfectant should be prepared with distilled water and used
on objects that have been cleaned of all gross soil, tissue, and
contaminants.
Chlorine is effective against a wide spectrum of bacteria,
entero-viruses, and spores, but chlorine solutions are unstable
and must be made daily. Chlorine can corrode metals and
soften plastics; it has a persistent odor and is irritating to eyes
and skin. These disadvantages usually rule out routine use of
chlorine solutions.
Iodine
Iodine is a faster disinfectant than a QAC or chlorine. The
free iodine forms salts with the bacterial protein, thus killing
the cell. Iodine is effective against vegetative bacteria,
spores, fungi, and certain viruses. Iodophors make effective
surface disinfectants and are easily prepared by mixing
iodine concentrate with softened or distilled water (hard
water and some concentrations of alcohol will inactivate the
iodine).
Other sources of disinfection include, but are not limited to,
ultraviolet light, mercuric salts, hot oil, flaming, phenolic
compounds, boiling water, and, more recently, microwaves.
EFFECTS OF STERILIZATION & DISINFECTION
ON ORTHODONTIC MATERIALS
Orthodontic wires
Smith and Von Fraunhofer in AJO 1992 studied the effect
of clinical use and various sterilization/disinfection protocols
on three types of nickel-titanium, and one type of β-titanium
and stainless steel arch wire. The sterilization/disinfection
procedures included,
 Disinfection → with an iodophor for 10 minutes
 Steam autoclave sterilization→ sterilization temperature
of 274° F (134.4° C) for 10 minutes.
 Cold sterilization→ freshly prepared sporocidin
solution for 6.75 hours as per the manufacturer's
recommendations.
 Dry heat sterilization→ sterilization temperature of
375° F (191° C) was maintained for 10 minutes.
The results indicated that load/deflection and tensile tests
showed no clinically significant difference between asreceived and used-then-disinfected/sterilized wires and
they concluded that nickel-titanium arch wires could be
recycled at least once.
Sunil Kapila, Haugen and Watanabe in AJO 1992
determined the effects of in vivo recycling interposed
by dry heat sterilization (together referred to as clinical
recycling, CR) on the load-deflection characteristics of
nickel-titanium alloy wires (Nitinol and NiTi).
The results indicated that both dry heat sterilization
(DHS) alone, as well as clinical recycling (CR),
produced significant changes in the loading and
unloading characteristics of Nitinol and NiTi wires.
However, the changes in the load-deflection
characteristics of these wires after DHS only were
relatively small, and the clinical significance of these
changes is open to question.
In contrast, the force levels during loading and
unloading were substantially increased for both types
of wires after CR.
They concluded that, clinical recycling appears to
reduce the "pseudoplasticity" and "pseudoelasticity"
of NiTi wires and increases the stiffness of both NiTi
and Nitinol wires.
Mayhew and Kusy in AJO 1988 studied the effects of
sterilization on the mechanical properties and the surface
topography of 0.017 ×0.025-inch Nitinol and Titanal arch wires.
Three approved heat sterilization methods were used namely,
 Dry heat→ applied at 180° C (355° F) for 60 minutes
 Formaldehyde alcohol vapor→ formaldehyde-alcohol vapor
pressure of 20 to 25 psi for 30 minutes at 132° C (270° F)
 Steam autoclave→ at 121° C (250° F) and 15 to 20 psi
pressure for 20 minutes
They concluded that neither the heat sterilization nor multiple
cycling procedures had a deleterious effect on the elastic moduli,
surface topography, or tensile properties of Nitinol or Titanal arch
wires.
The bending moduli and the tensile strengths were approximately
10% greater for Nitinol than for Titanal.
Orthodontic pliers

Vendrell and Hayden in AJO 2002 compared the wear of
orthodontic ligature-cutting pliers after multiple cycles of
cutting stainless steel ligature wire and sterilizing with dry
heat or steam autoclave. Fifty ligature-cutting pliers with
stainless steel inserts were randomly divided into 2 equal
groups to be sterilized in either dry heat or steam autoclave.
Each plier was subjected to a series of ligature wire cuts
followed by the assigned sterilization method. The amount
of wear at the tip of each plier in both groups was
measured with a stereomicroscope system and digital
photomicrography.
Orthodontic ligature-cutting pliers with stainless steel
inserts showed no significant difference in mean wear
whether sterilized with steam autoclave or dry heat. Steam
autoclave sterilization can be used with no significant
deleterious effects on pliers with stainless steel inserts.
Thank you
For more details please visit
www.indiandentalacademy.com

Más contenido relacionado

La actualidad más candente

Infection Control Measures in Covid 19
Infection Control Measures in Covid 19Infection Control Measures in Covid 19
Infection Control Measures in Covid 19Dr.Benny PV
 
Sterilisation protocol during prosthodontic treatment
Sterilisation protocol during prosthodontic treatmentSterilisation protocol during prosthodontic treatment
Sterilisation protocol during prosthodontic treatmentRani Ranabhatt, KGMC, Lucknow
 
Sterilization of operative & endodontic instruments
Sterilization of operative & endodontic instrumentsSterilization of operative & endodontic instruments
Sterilization of operative & endodontic instrumentsSk Aziz Ikbal
 
Sterilization and disinfection of orthodontic instruments /certified fixed or...
Sterilization and disinfection of orthodontic instruments /certified fixed or...Sterilization and disinfection of orthodontic instruments /certified fixed or...
Sterilization and disinfection of orthodontic instruments /certified fixed or...Indian dental academy
 
Sterilization in orthodontics
Sterilization in orthodonticsSterilization in orthodontics
Sterilization in orthodonticsTanvi Andrade
 
GUIDELINES FOR HEALTHCARE WORKERS ON INFECTION CONTROL
GUIDELINES FOR HEALTHCARE WORKERS ON INFECTION CONTROLGUIDELINES FOR HEALTHCARE WORKERS ON INFECTION CONTROL
GUIDELINES FOR HEALTHCARE WORKERS ON INFECTION CONTROLJithin Raj RN CIC
 
Sterilization and disinfection
Sterilization and disinfectionSterilization and disinfection
Sterilization and disinfectionSushant Kumar
 
Cleaning and Disinfection of Medical Instruments
Cleaning and Disinfection of Medical InstrumentsCleaning and Disinfection of Medical Instruments
Cleaning and Disinfection of Medical InstrumentsAli Kermanjani, PhD
 
Dental infection control post treatment last
Dental infection control post treatment lastDental infection control post treatment last
Dental infection control post treatment lastAmin Abusallamah
 
Sterilization final/ dental crown & bridge courses
Sterilization final/ dental crown & bridge coursesSterilization final/ dental crown & bridge courses
Sterilization final/ dental crown & bridge coursesIndian dental academy
 
Infection control in dentistry
Infection control in dentistryInfection control in dentistry
Infection control in dentistryRuhi Kashmiri
 
Environmental Decontamination
Environmental DecontaminationEnvironmental Decontamination
Environmental DecontaminationApollo Hospitals
 
Sterilisation & disinfection /certified fixed orthodontic courses by Indian d...
Sterilisation & disinfection /certified fixed orthodontic courses by Indian d...Sterilisation & disinfection /certified fixed orthodontic courses by Indian d...
Sterilisation & disinfection /certified fixed orthodontic courses by Indian d...Indian dental academy
 

La actualidad más candente (20)

Infection Control Measures in Covid 19
Infection Control Measures in Covid 19Infection Control Measures in Covid 19
Infection Control Measures in Covid 19
 
Disinfection seminar ppt
Disinfection seminar pptDisinfection seminar ppt
Disinfection seminar ppt
 
Sterilization in orthodontics (4)
Sterilization in orthodontics (4)Sterilization in orthodontics (4)
Sterilization in orthodontics (4)
 
Sterilisation protocol during prosthodontic treatment
Sterilisation protocol during prosthodontic treatmentSterilisation protocol during prosthodontic treatment
Sterilisation protocol during prosthodontic treatment
 
Sterilization of operative & endodontic instruments
Sterilization of operative & endodontic instrumentsSterilization of operative & endodontic instruments
Sterilization of operative & endodontic instruments
 
Sterilization and disinfection of orthodontic instruments /certified fixed or...
Sterilization and disinfection of orthodontic instruments /certified fixed or...Sterilization and disinfection of orthodontic instruments /certified fixed or...
Sterilization and disinfection of orthodontic instruments /certified fixed or...
 
Sterlization in orthodontics
Sterlization in orthodontics Sterlization in orthodontics
Sterlization in orthodontics
 
Sterilization in orthodontics
Sterilization in orthodonticsSterilization in orthodontics
Sterilization in orthodontics
 
GUIDELINES FOR HEALTHCARE WORKERS ON INFECTION CONTROL
GUIDELINES FOR HEALTHCARE WORKERS ON INFECTION CONTROLGUIDELINES FOR HEALTHCARE WORKERS ON INFECTION CONTROL
GUIDELINES FOR HEALTHCARE WORKERS ON INFECTION CONTROL
 
Sterilization and disinfection
Sterilization and disinfectionSterilization and disinfection
Sterilization and disinfection
 
Cleaning and Disinfection of Medical Instruments
Cleaning and Disinfection of Medical InstrumentsCleaning and Disinfection of Medical Instruments
Cleaning and Disinfection of Medical Instruments
 
Sterilization in orthodontics
Sterilization in orthodonticsSterilization in orthodontics
Sterilization in orthodontics
 
Standard safety measures
Standard safety measuresStandard safety measures
Standard safety measures
 
Dental infection control post treatment last
Dental infection control post treatment lastDental infection control post treatment last
Dental infection control post treatment last
 
Sterilization final/ dental crown & bridge courses
Sterilization final/ dental crown & bridge coursesSterilization final/ dental crown & bridge courses
Sterilization final/ dental crown & bridge courses
 
Infection control in dentistry
Infection control in dentistryInfection control in dentistry
Infection control in dentistry
 
Cleaning Decontamination, Disinfection and Sterilization Process
Cleaning Decontamination, Disinfection and Sterilization ProcessCleaning Decontamination, Disinfection and Sterilization Process
Cleaning Decontamination, Disinfection and Sterilization Process
 
Cleaning
CleaningCleaning
Cleaning
 
Environmental Decontamination
Environmental DecontaminationEnvironmental Decontamination
Environmental Decontamination
 
Sterilisation & disinfection /certified fixed orthodontic courses by Indian d...
Sterilisation & disinfection /certified fixed orthodontic courses by Indian d...Sterilisation & disinfection /certified fixed orthodontic courses by Indian d...
Sterilisation & disinfection /certified fixed orthodontic courses by Indian d...
 

Destacado

Busshelter da nang city unique ads
Busshelter da nang city   unique adsBusshelter da nang city   unique ads
Busshelter da nang city unique adsminhnghiemunique
 
Slide med fameca_catanduva
Slide med fameca_catanduvaSlide med fameca_catanduva
Slide med fameca_catanduvaPhotoCollective
 
Innovative Technologies and Student Success
Innovative Technologies and Student SuccessInnovative Technologies and Student Success
Innovative Technologies and Student SuccessHeather Lambert
 
[Ud] acuerdo prueba académica (proyecto csu)
[Ud] acuerdo prueba académica (proyecto csu)[Ud] acuerdo prueba académica (proyecto csu)
[Ud] acuerdo prueba académica (proyecto csu)gatoper
 
Rothesay Reads Article Pg2
Rothesay Reads Article Pg2Rothesay Reads Article Pg2
Rothesay Reads Article Pg2Tiffony Jacobs
 
Samsung SDS IAM & EMM for Healthcare
Samsung SDS IAM & EMM for HealthcareSamsung SDS IAM & EMM for Healthcare
Samsung SDS IAM & EMM for HealthcareSamsung SDS America
 

Destacado (12)

Busshelter da nang city unique ads
Busshelter da nang city   unique adsBusshelter da nang city   unique ads
Busshelter da nang city unique ads
 
Datasheet EWS350AP
Datasheet EWS350APDatasheet EWS350AP
Datasheet EWS350AP
 
Slide med fameca_catanduva
Slide med fameca_catanduvaSlide med fameca_catanduva
Slide med fameca_catanduva
 
Rigelyn
RigelynRigelyn
Rigelyn
 
Innovative Technologies and Student Success
Innovative Technologies and Student SuccessInnovative Technologies and Student Success
Innovative Technologies and Student Success
 
[Ud] acuerdo prueba académica (proyecto csu)
[Ud] acuerdo prueba académica (proyecto csu)[Ud] acuerdo prueba académica (proyecto csu)
[Ud] acuerdo prueba académica (proyecto csu)
 
01
0101
01
 
I-110799557
I-110799557I-110799557
I-110799557
 
Psychological management
Psychological managementPsychological management
Psychological management
 
Rothesay Reads Article Pg2
Rothesay Reads Article Pg2Rothesay Reads Article Pg2
Rothesay Reads Article Pg2
 
Jo Ann Cruz
Jo Ann CruzJo Ann Cruz
Jo Ann Cruz
 
Samsung SDS IAM & EMM for Healthcare
Samsung SDS IAM & EMM for HealthcareSamsung SDS IAM & EMM for Healthcare
Samsung SDS IAM & EMM for Healthcare
 

Similar a Sterilization /certified fixed orthodontic courses by Indian dental academy

Reusable Medical Devices (SOP).docx
Reusable Medical Devices (SOP).docxReusable Medical Devices (SOP).docx
Reusable Medical Devices (SOP).docxanjalatchi
 
6 what can you do in your clinic to prevent contamination and cross infection
6 what can you do in your clinic to prevent contamination and cross infection6 what can you do in your clinic to prevent contamination and cross infection
6 what can you do in your clinic to prevent contamination and cross infectionaakaricls
 
Cross Infection Control in operative dentistry and endodontics .pptx
Cross Infection Control in operative dentistry and endodontics .pptxCross Infection Control in operative dentistry and endodontics .pptx
Cross Infection Control in operative dentistry and endodontics .pptxamiramna351
 
INFECTION CONTROL PROTOCOL DURING COVID-19 IN DENTISTRY
INFECTION CONTROL PROTOCOL DURING COVID-19 IN DENTISTRYINFECTION CONTROL PROTOCOL DURING COVID-19 IN DENTISTRY
INFECTION CONTROL PROTOCOL DURING COVID-19 IN DENTISTRYJAMES RAJAN
 
Safety precautions in the clinic and laboratory.pptx
Safety precautions in the clinic and laboratory.pptxSafety precautions in the clinic and laboratory.pptx
Safety precautions in the clinic and laboratory.pptxMustafa Al-Ali
 
6 Basics of Infection Prevention and Control.pptREVISED (Day 1) (1) (1).ppt
6 Basics of Infection Prevention and Control.pptREVISED (Day 1) (1) (1).ppt6 Basics of Infection Prevention and Control.pptREVISED (Day 1) (1) (1).ppt
6 Basics of Infection Prevention and Control.pptREVISED (Day 1) (1) (1).pptjohnpickett25
 
Infection Control Guidelines for Dental Clinics [compatibility mode]
Infection Control Guidelines for Dental Clinics [compatibility mode]Infection Control Guidelines for Dental Clinics [compatibility mode]
Infection Control Guidelines for Dental Clinics [compatibility mode]drnahla
 
STERILISATION AND DISINFECTION IN DENTISTRY.pptx
STERILISATION   AND DISINFECTION   IN DENTISTRY.pptxSTERILISATION   AND DISINFECTION   IN DENTISTRY.pptx
STERILISATION AND DISINFECTION IN DENTISTRY.pptxDrRutikaNaik
 
sterilization in dentistry/Infection control
sterilization in dentistry/Infection controlsterilization in dentistry/Infection control
sterilization in dentistry/Infection controlDandu Prasad Reddy
 
Consistent practice protocol can break the chain of infection
Consistent practice protocol can break the chain of infectionConsistent practice protocol can break the chain of infection
Consistent practice protocol can break the chain of infectionmanish goutam
 
Aseptic technique-1.pptx
Aseptic technique-1.pptxAseptic technique-1.pptx
Aseptic technique-1.pptxbbb30706670
 
Sterilization and disinfection in dentistry
Sterilization and disinfection in dentistrySterilization and disinfection in dentistry
Sterilization and disinfection in dentistryDr.Swarneet Kakpure
 
Infection control protocol
Infection control protocolInfection control protocol
Infection control protocolAbhay Rajpoot
 
infection control protocols in hospital vap causi clapsi
infection control protocols in hospital vap causi clapsiinfection control protocols in hospital vap causi clapsi
infection control protocols in hospital vap causi clapsisohanrajchoudhary
 
BASICS TO FOOT CARE ppt FINAL2014.pdf
BASICS TO FOOT CARE ppt FINAL2014.pdfBASICS TO FOOT CARE ppt FINAL2014.pdf
BASICS TO FOOT CARE ppt FINAL2014.pdfMarcAndreo2
 
Asepsis, sterilization and infection control
Asepsis, sterilization and infection controlAsepsis, sterilization and infection control
Asepsis, sterilization and infection controlDr. Meenal Atharkar
 
Infection control in the dental clinic
Infection control in the dental clinicInfection control in the dental clinic
Infection control in the dental clinicHesham Dameer
 

Similar a Sterilization /certified fixed orthodontic courses by Indian dental academy (20)

Sterilization
Sterilization Sterilization
Sterilization
 
Reusable Medical Devices (SOP).docx
Reusable Medical Devices (SOP).docxReusable Medical Devices (SOP).docx
Reusable Medical Devices (SOP).docx
 
6 what can you do in your clinic to prevent contamination and cross infection
6 what can you do in your clinic to prevent contamination and cross infection6 what can you do in your clinic to prevent contamination and cross infection
6 what can you do in your clinic to prevent contamination and cross infection
 
Cross Infection Control in operative dentistry and endodontics .pptx
Cross Infection Control in operative dentistry and endodontics .pptxCross Infection Control in operative dentistry and endodontics .pptx
Cross Infection Control in operative dentistry and endodontics .pptx
 
INFECTION CONTROL PROTOCOL DURING COVID-19 IN DENTISTRY
INFECTION CONTROL PROTOCOL DURING COVID-19 IN DENTISTRYINFECTION CONTROL PROTOCOL DURING COVID-19 IN DENTISTRY
INFECTION CONTROL PROTOCOL DURING COVID-19 IN DENTISTRY
 
Safety precautions in the clinic and laboratory.pptx
Safety precautions in the clinic and laboratory.pptxSafety precautions in the clinic and laboratory.pptx
Safety precautions in the clinic and laboratory.pptx
 
10 infection control
10 infection control10 infection control
10 infection control
 
6 Basics of Infection Prevention and Control.pptREVISED (Day 1) (1) (1).ppt
6 Basics of Infection Prevention and Control.pptREVISED (Day 1) (1) (1).ppt6 Basics of Infection Prevention and Control.pptREVISED (Day 1) (1) (1).ppt
6 Basics of Infection Prevention and Control.pptREVISED (Day 1) (1) (1).ppt
 
Infection Control Guidelines for Dental Clinics [compatibility mode]
Infection Control Guidelines for Dental Clinics [compatibility mode]Infection Control Guidelines for Dental Clinics [compatibility mode]
Infection Control Guidelines for Dental Clinics [compatibility mode]
 
STERILISATION AND DISINFECTION IN DENTISTRY.pptx
STERILISATION   AND DISINFECTION   IN DENTISTRY.pptxSTERILISATION   AND DISINFECTION   IN DENTISTRY.pptx
STERILISATION AND DISINFECTION IN DENTISTRY.pptx
 
sterilization in dentistry/Infection control
sterilization in dentistry/Infection controlsterilization in dentistry/Infection control
sterilization in dentistry/Infection control
 
Consistent practice protocol can break the chain of infection
Consistent practice protocol can break the chain of infectionConsistent practice protocol can break the chain of infection
Consistent practice protocol can break the chain of infection
 
Infection control iyad
Infection control  iyadInfection control  iyad
Infection control iyad
 
Aseptic technique-1.pptx
Aseptic technique-1.pptxAseptic technique-1.pptx
Aseptic technique-1.pptx
 
Sterilization and disinfection in dentistry
Sterilization and disinfection in dentistrySterilization and disinfection in dentistry
Sterilization and disinfection in dentistry
 
Infection control protocol
Infection control protocolInfection control protocol
Infection control protocol
 
infection control protocols in hospital vap causi clapsi
infection control protocols in hospital vap causi clapsiinfection control protocols in hospital vap causi clapsi
infection control protocols in hospital vap causi clapsi
 
BASICS TO FOOT CARE ppt FINAL2014.pdf
BASICS TO FOOT CARE ppt FINAL2014.pdfBASICS TO FOOT CARE ppt FINAL2014.pdf
BASICS TO FOOT CARE ppt FINAL2014.pdf
 
Asepsis, sterilization and infection control
Asepsis, sterilization and infection controlAsepsis, sterilization and infection control
Asepsis, sterilization and infection control
 
Infection control in the dental clinic
Infection control in the dental clinicInfection control in the dental clinic
Infection control in the dental clinic
 

Más de Indian dental academy

Indian Dentist - relocate to united kingdom
Indian Dentist - relocate to united kingdomIndian Dentist - relocate to united kingdom
Indian Dentist - relocate to united kingdomIndian dental academy
 
1ST, 2ND AND 3RD ORDER BENDS IN STANDARD EDGEWISE APPLIANCE SYSTEM /Fixed ort...
1ST, 2ND AND 3RD ORDER BENDS IN STANDARD EDGEWISE APPLIANCE SYSTEM /Fixed ort...1ST, 2ND AND 3RD ORDER BENDS IN STANDARD EDGEWISE APPLIANCE SYSTEM /Fixed ort...
1ST, 2ND AND 3RD ORDER BENDS IN STANDARD EDGEWISE APPLIANCE SYSTEM /Fixed ort...Indian dental academy
 
Invisalign -invisible aligners course in india
Invisalign -invisible aligners course in india Invisalign -invisible aligners course in india
Invisalign -invisible aligners course in india Indian dental academy
 
Invisible aligners for your orthodontics pratice
Invisible aligners for your orthodontics praticeInvisible aligners for your orthodontics pratice
Invisible aligners for your orthodontics praticeIndian dental academy
 
Development of muscles of mastication / dental implant courses
Development of muscles of mastication / dental implant coursesDevelopment of muscles of mastication / dental implant courses
Development of muscles of mastication / dental implant coursesIndian dental academy
 
Corticosteriods uses in dentistry/ oral surgery courses  
Corticosteriods uses in dentistry/ oral surgery courses  Corticosteriods uses in dentistry/ oral surgery courses  
Corticosteriods uses in dentistry/ oral surgery courses  Indian dental academy
 
Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental ...
Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental ...Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental ...
Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental ...Indian dental academy
 
Diagnosis and treatment planning in completely endntulous arches/dental courses
Diagnosis and treatment planning in completely endntulous arches/dental coursesDiagnosis and treatment planning in completely endntulous arches/dental courses
Diagnosis and treatment planning in completely endntulous arches/dental coursesIndian dental academy
 
Properties of Denture base materials /rotary endodontic courses
Properties of Denture base materials /rotary endodontic coursesProperties of Denture base materials /rotary endodontic courses
Properties of Denture base materials /rotary endodontic coursesIndian dental academy
 
Use of modified tooth forms in complete denture occlusion / dental implant...
Use of modified  tooth forms  in  complete denture occlusion / dental implant...Use of modified  tooth forms  in  complete denture occlusion / dental implant...
Use of modified tooth forms in complete denture occlusion / dental implant...Indian dental academy
 
Dental luting cements / oral surgery courses  
Dental   luting cements / oral surgery courses  Dental   luting cements / oral surgery courses  
Dental luting cements / oral surgery courses  Indian dental academy
 
Dental casting alloys/ oral surgery courses  
Dental casting alloys/ oral surgery courses  Dental casting alloys/ oral surgery courses  
Dental casting alloys/ oral surgery courses  Indian dental academy
 
Dental casting investment materials/endodontic courses
Dental casting investment materials/endodontic coursesDental casting investment materials/endodontic courses
Dental casting investment materials/endodontic coursesIndian dental academy
 
Dental casting waxes/ oral surgery courses  
Dental casting waxes/ oral surgery courses  Dental casting waxes/ oral surgery courses  
Dental casting waxes/ oral surgery courses  Indian dental academy
 
Dental ceramics/prosthodontic courses
Dental ceramics/prosthodontic coursesDental ceramics/prosthodontic courses
Dental ceramics/prosthodontic coursesIndian dental academy
 
Dental implant/ oral surgery courses  
Dental implant/ oral surgery courses  Dental implant/ oral surgery courses  
Dental implant/ oral surgery courses  Indian dental academy
 
Dental perspective/cosmetic dentistry courses
Dental perspective/cosmetic dentistry coursesDental perspective/cosmetic dentistry courses
Dental perspective/cosmetic dentistry coursesIndian dental academy
 
Dental tissues and their replacements/ oral surgery courses  
Dental tissues and their replacements/ oral surgery courses  Dental tissues and their replacements/ oral surgery courses  
Dental tissues and their replacements/ oral surgery courses  Indian dental academy
 

Más de Indian dental academy (20)

Indian Dentist - relocate to united kingdom
Indian Dentist - relocate to united kingdomIndian Dentist - relocate to united kingdom
Indian Dentist - relocate to united kingdom
 
1ST, 2ND AND 3RD ORDER BENDS IN STANDARD EDGEWISE APPLIANCE SYSTEM /Fixed ort...
1ST, 2ND AND 3RD ORDER BENDS IN STANDARD EDGEWISE APPLIANCE SYSTEM /Fixed ort...1ST, 2ND AND 3RD ORDER BENDS IN STANDARD EDGEWISE APPLIANCE SYSTEM /Fixed ort...
1ST, 2ND AND 3RD ORDER BENDS IN STANDARD EDGEWISE APPLIANCE SYSTEM /Fixed ort...
 
Invisalign -invisible aligners course in india
Invisalign -invisible aligners course in india Invisalign -invisible aligners course in india
Invisalign -invisible aligners course in india
 
Invisible aligners for your orthodontics pratice
Invisible aligners for your orthodontics praticeInvisible aligners for your orthodontics pratice
Invisible aligners for your orthodontics pratice
 
online fixed orthodontics course
online fixed orthodontics courseonline fixed orthodontics course
online fixed orthodontics course
 
online orthodontics course
online orthodontics courseonline orthodontics course
online orthodontics course
 
Development of muscles of mastication / dental implant courses
Development of muscles of mastication / dental implant coursesDevelopment of muscles of mastication / dental implant courses
Development of muscles of mastication / dental implant courses
 
Corticosteriods uses in dentistry/ oral surgery courses  
Corticosteriods uses in dentistry/ oral surgery courses  Corticosteriods uses in dentistry/ oral surgery courses  
Corticosteriods uses in dentistry/ oral surgery courses  
 
Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental ...
Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental ...Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental ...
Cytotoxicity of silicone materials used in maxillofacial prosthesis / dental ...
 
Diagnosis and treatment planning in completely endntulous arches/dental courses
Diagnosis and treatment planning in completely endntulous arches/dental coursesDiagnosis and treatment planning in completely endntulous arches/dental courses
Diagnosis and treatment planning in completely endntulous arches/dental courses
 
Properties of Denture base materials /rotary endodontic courses
Properties of Denture base materials /rotary endodontic coursesProperties of Denture base materials /rotary endodontic courses
Properties of Denture base materials /rotary endodontic courses
 
Use of modified tooth forms in complete denture occlusion / dental implant...
Use of modified  tooth forms  in  complete denture occlusion / dental implant...Use of modified  tooth forms  in  complete denture occlusion / dental implant...
Use of modified tooth forms in complete denture occlusion / dental implant...
 
Dental luting cements / oral surgery courses  
Dental   luting cements / oral surgery courses  Dental   luting cements / oral surgery courses  
Dental luting cements / oral surgery courses  
 
Dental casting alloys/ oral surgery courses  
Dental casting alloys/ oral surgery courses  Dental casting alloys/ oral surgery courses  
Dental casting alloys/ oral surgery courses  
 
Dental casting investment materials/endodontic courses
Dental casting investment materials/endodontic coursesDental casting investment materials/endodontic courses
Dental casting investment materials/endodontic courses
 
Dental casting waxes/ oral surgery courses  
Dental casting waxes/ oral surgery courses  Dental casting waxes/ oral surgery courses  
Dental casting waxes/ oral surgery courses  
 
Dental ceramics/prosthodontic courses
Dental ceramics/prosthodontic coursesDental ceramics/prosthodontic courses
Dental ceramics/prosthodontic courses
 
Dental implant/ oral surgery courses  
Dental implant/ oral surgery courses  Dental implant/ oral surgery courses  
Dental implant/ oral surgery courses  
 
Dental perspective/cosmetic dentistry courses
Dental perspective/cosmetic dentistry coursesDental perspective/cosmetic dentistry courses
Dental perspective/cosmetic dentistry courses
 
Dental tissues and their replacements/ oral surgery courses  
Dental tissues and their replacements/ oral surgery courses  Dental tissues and their replacements/ oral surgery courses  
Dental tissues and their replacements/ oral surgery courses  
 

Último

Oppenheimer Film Discussion for Philosophy and Film
Oppenheimer Film Discussion for Philosophy and FilmOppenheimer Film Discussion for Philosophy and Film
Oppenheimer Film Discussion for Philosophy and FilmStan Meyer
 
Q-Factor HISPOL Quiz-6th April 2024, Quiz Club NITW
Q-Factor HISPOL Quiz-6th April 2024, Quiz Club NITWQ-Factor HISPOL Quiz-6th April 2024, Quiz Club NITW
Q-Factor HISPOL Quiz-6th April 2024, Quiz Club NITWQuiz Club NITW
 
week 1 cookery 8 fourth - quarter .pptx
week 1 cookery 8  fourth  -  quarter .pptxweek 1 cookery 8  fourth  -  quarter .pptx
week 1 cookery 8 fourth - quarter .pptxJonalynLegaspi2
 
Team Lead Succeed – Helping you and your team achieve high-performance teamwo...
Team Lead Succeed – Helping you and your team achieve high-performance teamwo...Team Lead Succeed – Helping you and your team achieve high-performance teamwo...
Team Lead Succeed – Helping you and your team achieve high-performance teamwo...Association for Project Management
 
Congestive Cardiac Failure..presentation
Congestive Cardiac Failure..presentationCongestive Cardiac Failure..presentation
Congestive Cardiac Failure..presentationdeepaannamalai16
 
Measures of Position DECILES for ungrouped data
Measures of Position DECILES for ungrouped dataMeasures of Position DECILES for ungrouped data
Measures of Position DECILES for ungrouped dataBabyAnnMotar
 
Mythology Quiz-4th April 2024, Quiz Club NITW
Mythology Quiz-4th April 2024, Quiz Club NITWMythology Quiz-4th April 2024, Quiz Club NITW
Mythology Quiz-4th April 2024, Quiz Club NITWQuiz Club NITW
 
Concurrency Control in Database Management system
Concurrency Control in Database Management systemConcurrency Control in Database Management system
Concurrency Control in Database Management systemChristalin Nelson
 
Mental Health Awareness - a toolkit for supporting young minds
Mental Health Awareness - a toolkit for supporting young mindsMental Health Awareness - a toolkit for supporting young minds
Mental Health Awareness - a toolkit for supporting young mindsPooky Knightsmith
 
ClimART Action | eTwinning Project
ClimART Action    |    eTwinning ProjectClimART Action    |    eTwinning Project
ClimART Action | eTwinning Projectjordimapav
 
Grade 9 Quarter 4 Dll Grade 9 Quarter 4 DLL.pdf
Grade 9 Quarter 4 Dll Grade 9 Quarter 4 DLL.pdfGrade 9 Quarter 4 Dll Grade 9 Quarter 4 DLL.pdf
Grade 9 Quarter 4 Dll Grade 9 Quarter 4 DLL.pdfJemuel Francisco
 
ESP 4-EDITED.pdfmmcncncncmcmmnmnmncnmncmnnjvnnv
ESP 4-EDITED.pdfmmcncncncmcmmnmnmncnmncmnnjvnnvESP 4-EDITED.pdfmmcncncncmcmmnmnmncnmncmnnjvnnv
ESP 4-EDITED.pdfmmcncncncmcmmnmnmncnmncmnnjvnnvRicaMaeCastro1
 
4.16.24 Poverty and Precarity--Desmond.pptx
4.16.24 Poverty and Precarity--Desmond.pptx4.16.24 Poverty and Precarity--Desmond.pptx
4.16.24 Poverty and Precarity--Desmond.pptxmary850239
 
Multi Domain Alias In the Odoo 17 ERP Module
Multi Domain Alias In the Odoo 17 ERP ModuleMulti Domain Alias In the Odoo 17 ERP Module
Multi Domain Alias In the Odoo 17 ERP ModuleCeline George
 
How to Make a Duplicate of Your Odoo 17 Database
How to Make a Duplicate of Your Odoo 17 DatabaseHow to Make a Duplicate of Your Odoo 17 Database
How to Make a Duplicate of Your Odoo 17 DatabaseCeline George
 
Q-Factor General Quiz-7th April 2024, Quiz Club NITW
Q-Factor General Quiz-7th April 2024, Quiz Club NITWQ-Factor General Quiz-7th April 2024, Quiz Club NITW
Q-Factor General Quiz-7th April 2024, Quiz Club NITWQuiz Club NITW
 
DIFFERENT BASKETRY IN THE PHILIPPINES PPT.pptx
DIFFERENT BASKETRY IN THE PHILIPPINES PPT.pptxDIFFERENT BASKETRY IN THE PHILIPPINES PPT.pptx
DIFFERENT BASKETRY IN THE PHILIPPINES PPT.pptxMichelleTuguinay1
 
Using Grammatical Signals Suitable to Patterns of Idea Development
Using Grammatical Signals Suitable to Patterns of Idea DevelopmentUsing Grammatical Signals Suitable to Patterns of Idea Development
Using Grammatical Signals Suitable to Patterns of Idea Developmentchesterberbo7
 
Q4-PPT-Music9_Lesson-1-Romantic-Opera.pptx
Q4-PPT-Music9_Lesson-1-Romantic-Opera.pptxQ4-PPT-Music9_Lesson-1-Romantic-Opera.pptx
Q4-PPT-Music9_Lesson-1-Romantic-Opera.pptxlancelewisportillo
 

Último (20)

Oppenheimer Film Discussion for Philosophy and Film
Oppenheimer Film Discussion for Philosophy and FilmOppenheimer Film Discussion for Philosophy and Film
Oppenheimer Film Discussion for Philosophy and Film
 
Q-Factor HISPOL Quiz-6th April 2024, Quiz Club NITW
Q-Factor HISPOL Quiz-6th April 2024, Quiz Club NITWQ-Factor HISPOL Quiz-6th April 2024, Quiz Club NITW
Q-Factor HISPOL Quiz-6th April 2024, Quiz Club NITW
 
week 1 cookery 8 fourth - quarter .pptx
week 1 cookery 8  fourth  -  quarter .pptxweek 1 cookery 8  fourth  -  quarter .pptx
week 1 cookery 8 fourth - quarter .pptx
 
Team Lead Succeed – Helping you and your team achieve high-performance teamwo...
Team Lead Succeed – Helping you and your team achieve high-performance teamwo...Team Lead Succeed – Helping you and your team achieve high-performance teamwo...
Team Lead Succeed – Helping you and your team achieve high-performance teamwo...
 
Congestive Cardiac Failure..presentation
Congestive Cardiac Failure..presentationCongestive Cardiac Failure..presentation
Congestive Cardiac Failure..presentation
 
Measures of Position DECILES for ungrouped data
Measures of Position DECILES for ungrouped dataMeasures of Position DECILES for ungrouped data
Measures of Position DECILES for ungrouped data
 
Mythology Quiz-4th April 2024, Quiz Club NITW
Mythology Quiz-4th April 2024, Quiz Club NITWMythology Quiz-4th April 2024, Quiz Club NITW
Mythology Quiz-4th April 2024, Quiz Club NITW
 
Concurrency Control in Database Management system
Concurrency Control in Database Management systemConcurrency Control in Database Management system
Concurrency Control in Database Management system
 
Mental Health Awareness - a toolkit for supporting young minds
Mental Health Awareness - a toolkit for supporting young mindsMental Health Awareness - a toolkit for supporting young minds
Mental Health Awareness - a toolkit for supporting young minds
 
ClimART Action | eTwinning Project
ClimART Action    |    eTwinning ProjectClimART Action    |    eTwinning Project
ClimART Action | eTwinning Project
 
Grade 9 Quarter 4 Dll Grade 9 Quarter 4 DLL.pdf
Grade 9 Quarter 4 Dll Grade 9 Quarter 4 DLL.pdfGrade 9 Quarter 4 Dll Grade 9 Quarter 4 DLL.pdf
Grade 9 Quarter 4 Dll Grade 9 Quarter 4 DLL.pdf
 
ESP 4-EDITED.pdfmmcncncncmcmmnmnmncnmncmnnjvnnv
ESP 4-EDITED.pdfmmcncncncmcmmnmnmncnmncmnnjvnnvESP 4-EDITED.pdfmmcncncncmcmmnmnmncnmncmnnjvnnv
ESP 4-EDITED.pdfmmcncncncmcmmnmnmncnmncmnnjvnnv
 
4.16.24 Poverty and Precarity--Desmond.pptx
4.16.24 Poverty and Precarity--Desmond.pptx4.16.24 Poverty and Precarity--Desmond.pptx
4.16.24 Poverty and Precarity--Desmond.pptx
 
Multi Domain Alias In the Odoo 17 ERP Module
Multi Domain Alias In the Odoo 17 ERP ModuleMulti Domain Alias In the Odoo 17 ERP Module
Multi Domain Alias In the Odoo 17 ERP Module
 
How to Make a Duplicate of Your Odoo 17 Database
How to Make a Duplicate of Your Odoo 17 DatabaseHow to Make a Duplicate of Your Odoo 17 Database
How to Make a Duplicate of Your Odoo 17 Database
 
Q-Factor General Quiz-7th April 2024, Quiz Club NITW
Q-Factor General Quiz-7th April 2024, Quiz Club NITWQ-Factor General Quiz-7th April 2024, Quiz Club NITW
Q-Factor General Quiz-7th April 2024, Quiz Club NITW
 
DIFFERENT BASKETRY IN THE PHILIPPINES PPT.pptx
DIFFERENT BASKETRY IN THE PHILIPPINES PPT.pptxDIFFERENT BASKETRY IN THE PHILIPPINES PPT.pptx
DIFFERENT BASKETRY IN THE PHILIPPINES PPT.pptx
 
Using Grammatical Signals Suitable to Patterns of Idea Development
Using Grammatical Signals Suitable to Patterns of Idea DevelopmentUsing Grammatical Signals Suitable to Patterns of Idea Development
Using Grammatical Signals Suitable to Patterns of Idea Development
 
Q4-PPT-Music9_Lesson-1-Romantic-Opera.pptx
Q4-PPT-Music9_Lesson-1-Romantic-Opera.pptxQ4-PPT-Music9_Lesson-1-Romantic-Opera.pptx
Q4-PPT-Music9_Lesson-1-Romantic-Opera.pptx
 
Faculty Profile prashantha K EEE dept Sri Sairam college of Engineering
Faculty Profile prashantha K EEE dept Sri Sairam college of EngineeringFaculty Profile prashantha K EEE dept Sri Sairam college of Engineering
Faculty Profile prashantha K EEE dept Sri Sairam college of Engineering
 

Sterilization /certified fixed orthodontic courses by Indian dental academy

  • 1. STERILIZATION & DISINFECTION OF ORTHODONTIC INSTRUMENTS AND CONSUMABLES INDIAN DENTAL ACADEMY Leader in continuing dental education www.indiandentalacademy.com
  • 2. CONTENTS  INTRODUCTION  STERILIZATION AND DISINFECTION- DEFINITION  INACTIVATION OF MICROORGANISMS  INFECTION CONTROL • BARRIER PROTECTION • ENVIRONMENTAL SURFACE DISINFECTION  CONTAMINATION VEHICLES  STERILIZATION AND ITS METHODS  DISINFECTION AND ITS METHODS  EFFECTS OF STERILIZATION & DISINFECTION ON ORTHODONTIC MATERIALS Effects on orthodontic wires Effects on orthodontic pliers
  • 3. INTRODUCTION For protection of both the doctor and patient, sterilization techniques are of utmost importance in preventing the spread of infectious disease. This is of special significance in dentistry because more microorganisms are found in the oral cavity than in any other part of the body. With the increasing number of adult patients and diverse life-styles, the orthodontist is more at risk than ever to exposure to serious pathogens and must take precautions to guard against their transfer. Orthodontists have the second highest incidence of hepatitis B among dental professionals (Starnbach, 1980). Saliva is about half as infectious as blood, and the most likely modes of transmission in dental offices are through puncture wounds, skin abrasions, or lesions.
  • 4. Dental aerosols, splattering, and instrument contamination can also transmit the virus, which can survive for several weeks at room temperature. Hepatitis B (serum hepatitis), herpes, and AIDS viruses are certainly the more serious diseases of many that can be contracted in an orthodontic office. The HTLV-III (AIDS) virus is more fragile and less infective; transmission is most likely to occur after repeated blood-to-blood or blood-to-mucosa contact or sharing of hypodermic needles. Still, the increasing incidence of AIDS, as well as of hepatitis B, has made orthodontists even more aware of the necessity of decontaminating instruments and surfaces.
  • 5. STERILIZATION: Sterilization is a process by which articles are freed of all microorganisms both in vegetative or spore state. DISINFECTION: Disinfection is a process of destruction of pathogenic organisms capable of giving rise to infection. ANTISEPTIC: It means prevention of infection by inhibiting growth of bacteria.
  • 6. INACTIVATION OF MICROORGANISMS Hepatitis virus→ Kobayashi and associates (1984), using direct chimpanzee inoculation as an assay method, found that 0.1% aqueous glutaraldehyde at 24° C for 5 minutes, 1% aqueous glutaraldehyde at 24° C for 1 minute, 80% ethanol at 11° C for 2 minutes, and heat at 98° C for 2 minutes are all able to inactivate hepatitis B virus in human plasma.
  • 7. AIDS virus→ AIDS virus is inactivated after treatment with 50% ethyl alcohol, 35% isopropyl alcohol, 0.1% household bleach, Lysol, and 0.3% H2O2 at room temperature (21° to 25° C) for 2 to 10 minutes. Heating at 56° C for 30 minutes inactivates the AIDS virus, and one investigator found the virus titer level dropped to undetectable levels after 10 minutes of heating at 56° C in the presence of serum. HTLV III/LAV has unusual stability at room temperature. Infective viral particles have survived for more than a week at 20° to 22° C in either dry form or liquid medium.
  • 8. INFECTION CONTROL An infection-control program comprises two distinct areas, 1) Exposure control and 2) Hazard communication Exposure control covers sterilization and disinfection, waste management, and employee safety and education, including personal protective equipment and bodily-fluid-exposure protocols. Hazard communication requirements include drills for hazard communication plans (chemical spills, emergency first aid, and fire or tornado evacuation), secondary labeling of hazardous chemicals, Material Safety Data Sheets, x-ray updates, and properly displayed state and federal posters.
  • 9. A chair side assistant or records technician should be appointed as the office Environmental Safety Coordinator. The Environmental Safety Coordinator’s responsibilities include: • Keeping all manuals and secondary labeling current • Conducting staff training annually, or as procedures change • Keeping records of annual hepatitis-B vaccinations for patient-contact employees
  • 10. • Conducting and documenting weekly spore tests or other appropriate monitoring of sterilization equipment • Maintaining infection-control inventory • Cleaning equipment nightly, weekly, or monthly as required • Filing exposure-incident reports The main tasks of infection control are implementation of barriers to the spread of pathogens, disinfection of environmental surfaces, and sterilization
  • 11. Barrier Protection Long-sleeved, jewel-neck clinic jackets should be worn in the laboratory and operatory. These jackets should be replaced daily, or when visibly soiled, and worn only in the office. Masks and protective eyewear are required during bonding and debonding procedures to protect against aerosols of blood and saliva. Face shields or side shields should be added to personal eyeglasses. Masks and face shields are required whenever a handpiece is used.
  • 12. Patients should be provided eyewear during any procedure with a risk of eye injury from debris or chemical agents. Latex gloves must be worn for all patient procedures, and changed between patients. Gloves must also be removed and replaced before handling materials such as charts, study casts, and radiographs.
  • 13. Environmental Surface Disinfection In the operatory, use a spray-wipespray technique with a phenol solution that cleans, disinfects, and deodorizes. After each patient, any exposed surfaces—chairside units, pencils, pens, counters, chairs—are sprayed with phenol and wiped down with a paper towel. This reduces the number of microbes that must be killed and removes any bodily fluids that may insulate the remaining microbes. The surfaces are then resprayed and allowed to dry for 10 minutes, after which excess moisture is wiped away. Dental chairs only have to be sprayed after handpiece procedures that can create aerosols.
  • 14. Clear plastic wrap should be used over the handpiece consoles and over all chairside switches or handles. In the laboratory, alginate impressions are rinsed with water after removal from the patient’s mouth, sprayed with Biocide, and placed in reclosable sandwich bags. (Phenols such as Birex should not be used because they can distort impressions). The impressions are rinsed again with water and dried before pouring, and they are always handled with latex gloves. Finished appliances are placed in sandwich bags or retainer boxes until delivery.
  • 15. CONTAMINATION VEHICLES Payne in AJO 1986 explained the three possible pathways of cross-contamination and its handling methods as 1) Critical— Instruments that penetrate the mucosa must be sterilized. 2) Semicritical— Instruments that touch the mucosa should be sterilized. 3) Least critical— Surfaces touched during treatment should be disinfected. Using this as a guide, scalers, scalpels, and other tissuecutting instruments must be sterilized. Other hand instruments and pliers should be sterilized. Work surfaces, triplex syringe handles, operating light handles, and other environmental surfaces should be disinfected.
  • 16. Shanon T. Kirchhof in JCO 1987 pointed out that to prevent the spread of infection in an orthodontic office, there are three main contamination vehicles that must be effectively sterilized, 1) Instruments contaminated with blood or saliva 2) Surfaces contaminated with blood or saliva, and 3) Staff members' hands Instruments Instruments requiring sterilization include mirrors, pliers, scalers, banding and bonding instruments, bands, impression trays, cotton pliers, and ligature directors. Some, including plastic band seating instruments, cheek retractors, mirrors, and bite planes, cannot withstand heat sterilization.
  • 17. If ethylene oxide is not being used and the instrument would be damaged by heat sterilization, it should be thoroughly debrided and submerged in a fresh solution of glutaraldehyde or formaldehyde for one to 10 hours. The length of time depends on the sterilant used and the temperature of the solution. Surfaces Surfaces that cannot be sterilized should be effectively disinfected. These surfaces include bracket trays, air/water syringes, saliva ejector handles, chair control buttons, operatory light handles, sink handles, supply drawers, and chair armrests and headrests. Each office can prepare its own list of operatory surfaces.
  • 18. Proper office and equipment design will reduce the number of surfaces that need disinfecting. Foot-activated chair controls and foot- or knee-operated sinks and soap dispensers are a few ways to avoid hand contact. Contaminated surfaces can be scrubbed with iodophorsoaked 4" × 4" gauze pads and allowed to dry. Gauze soaked with 70 percent alcohol can be used to remove the residue after drying. The iodine solution has a built-in antimicrobial activity indicator— when it turns from amber to clear, it should be replaced. Iodophors have a slight allergenic reaction with skin and can stain light-colored surfaces after repeated use.
  • 19. The recently introduced Vitawipes are disposable disinfectant cloths containing polyhydrochloride (3.2 percent), alkyl dimethyl, benzyl ammonium chloride (7.1 percent), and inert ingredients (89.7 percent). A cloth is dampened with water and used to wipe contaminated surfaces. It does not kill bacterial spores, mycobacterium tuberculosis, or hepatitis B virus on contact, but disinfects by physically removing the potential pathogens. The cloth is disposed of after an indicator strip changes color or after wiping a surface contaminated with hepatitis B virus. The wipes are easy to use and show minimal reaction with skin, but they have not been fully tested.
  • 20. Hands Hands are the most common link in the contamination chain. Proper handwashing reduces the number of potential pathogens, but it does not sterilize the skin. The hands, fingers, and fingernail bed have breaks in the epidermis that cannot be seen with the naked eye. Patients' blood has been retained under the fingernails of unprotected hands for five or more days after patient contact. Hands should be washed for 20 seconds with an effective antimicrobial soap. The soap should have residual action because of the warm, moist environment created by gloves.
  • 21. Gloves provide the necessary physical barrier from pathogenic organisms and should be worn while treating all patients. The smooth surface of gloves also allows more effective handwashing between patients. Gloves must fit properly to prevent loss of dexterity. Hand cream can be applied before glove use if one is sensitive to talc or cornstarch. Masks and eye protection are recommended to protect from aerosols and particle debris produced by handpieces, air/water syringes, trimming and polishing burs, and other equipment.
  • 22. Fernando Ascencio in JCO 1998 studied whether orthodontic marking pencils can pick up and transfer bacteria from patient to patient during typical orthodontic procedures. The results showed that marking pencils can transfer bacteria from contaminated archwires. He concluded that conventional orthodontic marking pencils cannot be autoclaved. Gas sterilization is effective in killing bacteria, but is also costly and difficult, making it impractical for orthodontic offices.
  • 23. Soaking or spraying the tips of marking pencils with disinfectants could be more effective than wiping, but this method is unlikely to gain acceptance from practitioners. The only sure way to avoid potential cross -contamination is to use the inexpensive disposable markers available from orthodontic supply companies. Metal and elastomeric ligatures are potential agents in the transmission of infectious diseases. Mulick (1986) recommended single-use dispensing of elastomeric materials to eliminate contact of canes or sticks with contaminated hands.
  • 24. Schneeweiss in JCO 1993 described a method of cutting elastomeric modules into smaller sections and covering them with clear tubing, which could then be cold sterilized. During archwire placement, the operator contacts only the outside tubing while removing ligatures More recently, dispensers have been introduced onto the market, but the effectiveness of such dispensers in controlling cross -infection has yet to be fully evaluated.
  • 25. Takla in JCO 1998 evaluated the effectiveness of a new elastomeric module dispenser in reducing bacterial contamination, as compared with the existing method of storing and dispensing elastomeric modules on canes. He concluded that the Alastik elastomeric module dispenser proved to be efficient in limiting crossinfection through single-use dispensing, although it did not offer protection against handling and environmental factors, such as dust. Alastik elastomeric module dispenser
  • 26. STERILIZATION AND ITS METHODS Sterilization destroys all microorganisms, including viruses and spore forms, and usually involves the use of heat. To sterilize properly and avoid damaging instruments, specific steps must be followed. After each patient procedure, sharps are discarded in a sharps container, and disposable items in a recessed, plastic-lined wastebasket. Plastic items that cannot be autoclaved are placed overnight in Procide, an immersion sterilization solution. Heat-sterilizable cheek retractors are also immersed in Procide, since they tend to turn milky after autoclaving. Handpieces and photographic mirrors are sterilized in a Kavo-Klave.
  • 27. Rinsing reduces the amount of contaminating solids before the instruments are debrided with an ultrasonic cleaner. If infected material is not removed, the time required to destroy all microorganisms may be increased. Heavy gloves should be worn to protect personnel from possible contamination. Depending on the sterilization method, the instruments are then placed on a rack or towel or are wrapped.
  • 28. Plier racks and instruments are placed in a wire basket and run through an ultrasonic cleaner containing a rustinhibiting Non-Ionic Multipurpose Ultra-sonic Cleaner. Ultrasonic solutions should be changed daily and covered during cleaning to reduce aerosols. Instruments and pliers should not be rinsed after ultrasonic cleaning. Any dried blood that remains must be scrubbed off by hand, and the ultrasonic cleaning must then be repeated before sterilization.
  • 29. Alternatively instruments are dipped in a Sodium Nitrite Rust Inhibitor, drained of excess sodium nitrite, and made ready for sterilization. After ultrasonic cleaning, the next step is to thoroughly dry the instruments. Hinged instruments may be "milked" with a water-soluble oil dip to lubricate them and prevent corrosion. The sterilization methods require different combinations of time and temperature.
  • 30. Sterilization can be accomplished in one of several ways. Some of the most common ways that are followed in orthodontic practice include, 1) Steam autoclave sterilization 2) Dry heat sterilization 3) Glass bead sterilization 4) Chemical vapour sterilization 5) Ethylene oxide sterilization
  • 31. Steam autoclave sterilization Steam sterilization (autoclave) uses saturated water vapor at 240° F, with 15 pounds of pressure for 15 to 40 minutes. The time can be reduced to three minutes by raising the pressure to 30 psi and the temperature to 270° F. More time is required for heavily wrapped loads of instruments. Sterilization can be verified with indicators and spore tests. It is a time-tested method that has little value for orthodontists because it severely rusts pliers and damages cutting edges. The corrosion may be reduced by dipping the instruments in a milk-like emulsion of oil in water prior to sterilization.
  • 32. Dry heat sterilization Dry heat provides a relatively low-cost sterilization procedure. Dry heat ovens require one hour at 320-340°F for sterilization. Wrapping or increasing the number of instruments increases the time required. It has two major drawbacks. It requires from 1 to 2 hours at 320° F for a complete cycle— far too long being practical for inventory considerations. A lesser problem is the tendency for the air to stratify and cause uneven temperatures that result in a lack of sterility.
  • 33. Glass bead sterilization Heat transfer media (salt or glass bead sterilizers) have been shown effective against most organisms and spores. There is some evidence that reliable, broad-spectrum sterilization occurs only with small instruments. Bulky instruments are not recommended because they may cool the medium below the reliable temperature for sterilization.
  • 34. Glass bead sterilization uses small glass beads ranging from 1.2 to 1.5 mm in diameter. The suggested heating range is 424° to 450° F31 (217° to 232° C) for 3 to 5 seconds but not exceeding 482° F (250° C). A relationship exists between the size and working surface of an instrument and the temperatures attained in the bead sterilizer. The larger the instrument, the longer the heat-up time required. A narrow, deep well is preferable to a wide, shallow one; instruments should be placed deep and near the sides of the wall for best results.
  • 35. Gerald E. Smith in AJO 1986 determined the capability of glass bead sterilization in sterilizing orthodontic bands and compared bead sterilization to other methods of cleansing and disinfecting orthodontic bands used in the office setting. The bands were placed one at a time into a 226° C bead sterilizer for 15, 30, 45, and 60 seconds. The results indicated that 15 seconds is required to sterilize bacteria and 45 seconds required for spores. A longer bead sterilization time is required if more than one band at a time is placed in the well. Twice the amount of time or 90 seconds is needed if five bands are inserted simultaneously due to the limited size of the well and fluctuation in temperatures with depth.
  • 36. Other methods of disinfecting orthodontic bands, including tap water rinse, soap scrub, 30-minute alcohol soak, and alcohol flame, are not adequate to prevent growth of Staphylococcus albus and Bacillus subtilis cultures with one exception— alcohol flame appears capable of preventing growth on bands inoculated with bacteria.
  • 37. Chemical vapour sterilization Unsaturated chemical vapor sterilization (Chemi-clave) is a suitable method for orthodontic instruments. Chemical vapor sterilizers use formaldehyde, alcohols, and water. The clean, dry, unwrapped instruments are set on a tray in the chamber, and the unit is set at 270° F at 20-40 psi for 20 minutes.
  • 38. When the chamber is opened, the toxic formaldehyde vapor must be vented to the outside. Because an unsaturated vapor is used, rusting is not a problem. It has a cycling time that is practical for an orthodontic office. Its chief drawback is a chemical odor that, although not harmful, requires adequate ventilation.
  • 39. Ethylene oxide sterilization Ethylene oxide is useful in as much as towels, and metal and plastic instruments may be sterilized simultaneously. It is the only major sterilization technique that does not require heat above room temperature. Hyperbaric gas (ethylene oxide) sterilization is recommended for instruments that are prone to corrosion or heat damage. However, the process is slow and costly, and the effluent gas is highly toxic.
  • 40. Standard treatment varies with temperature: 12 hours are required at room temperature, four hours at 56°C. It has the disadvantages of being toxic, allergenic, requiring a long exposure time, and is explosive if mixed with air. It is therefore combined with an inert gas such as carbon dioxide to render it nonexplosive. Another disadvantage is that materials retain varying amounts of ethylene oxide gas after removal from the sterilizer, and this must be allowed to dissipate before use.
  • 41. Glutaraldehydes— alkaline, acidic, and heat-potentiated— are effective sterilants for instruments other than pliers, but only when used for 6 to 10 hours. Again, this is an impractical cycle time. Their best use is for plastics and other heat-sensitive items. After heat sterilization, each rack of sterilized instruments is then kept in a cool-down drawer, which is lined with plastic laminate to control moisture, until the instruments and pliers can be returned to storage. It is important that cutting instruments be rotated for periodic resharpening. Plier hinges can be lubricated as necessary.
  • 42. The most common inefficiencies in orthodontic sterilization procedures are overhandling of instruments and improper chairside clean-up. Contributing factors can include mislocation of the sterilization area, poor flow control of breakdown and sterilization, excess instrumentation, and poor storage organization. Many orthodontists practice overkill procedures that are not required by any regulations, such as bagging individual instruments, wearing masks for all procedures, spraying chairs after every patient, maintaining an in-house laundry, and buying several different products when one will do the job.
  • 43. DISINFECTION AND ITS METHODS A number of methods have been used in orthodontic offices to disinfect instruments and environmental surfaces. A 70% alcohol solution has been the most widely used even though the least effective. A 1% solution of sodium hypochlorite (bleach) is very effective, but hard on the skin and has an unpleasant odor. The iodophors are the best choice. They are inexpensive, have residual effectiveness, and are easy to use and store. Their single drawback is the light brown residue left on surfaces, which disappears as the compound oxidizes. It does not stain as iodine does. These solutions can be made by diluting 1 oz povidone-iodine preparation in 16 oz of 70% isopropyl alcohol. They are also available in dry form to be diluted with water.
  • 44. Quaternary ammonium compounds (QAC) A quaternary ammonium compound (QAC or "quat") reduces the surface tension between bacteria and an object, thus disrupting the bacterial cell wall. Concentration, degree of contamination, level and extent of contact, and presence of other compounds all play a role in QAC effectiveness. Cotton, air, gross soil, or unusually heavy bacteria can prevent contact of the disinfectant with the cell wall. Combining several disinfectants— for example, a QAC with a phenolic compound containing an anionic detergent— can cause them to neutralize each other.
  • 45. Quaternary ammonium compounds (QAC) are used routinely for hand instruments because the metal remains bright and shows no sign of corrosion. They have a pleasant odor and a short time cycle. Disadvantages of QAC include, their inactivation by soap, reduced effectiveness in the presence of organic matter, incompatibility with many chemicals found in dental offices, and limited effectiveness against gramnegative organisms, spores, and viruses.
  • 46. Phenol Phenol is not itself used as a disinfectant, but many disinfectants have been derived from it. At high concentrations, phenol is a rapid protoplasmic poison that penetrates the cell wall and precipitates the cell protein. The effectiveness of phenolic compounds depends on contact with the bacterial cell. These compounds are effective against vegetative bacteria, lipophilic viruses, and tuberculosis, but not against bacterial spores or hydrophilic viruses.
  • 47. Alcohol Alcohol is a moderate disinfectant that behaves similarly to a QAC. Absolute alcohol is less effective than a 70 percent aqueous solution. Isopropyl alcohol is more effective than ethyl alcohol, but neither is effective against spores. Alcohol is generally bacteriocidal against vegetative forms. However, the American Dental Association (ADA) does not recommend alcohols, QACs, or phenolic compounds for use in dentistry, because they are nonsporicidal and ineffective against hepatitis B virus.
  • 48. Chlorine Chlorine in aqueous solutions, even in minute amounts, is rapidly bacteriocidal. The exact mechanism of this activity is not known, but theories range from cell wall damage and enzyme system blockage to protoplasmic poisoning. Chlorine disinfectant should be prepared with distilled water and used on objects that have been cleaned of all gross soil, tissue, and contaminants. Chlorine is effective against a wide spectrum of bacteria, entero-viruses, and spores, but chlorine solutions are unstable and must be made daily. Chlorine can corrode metals and soften plastics; it has a persistent odor and is irritating to eyes and skin. These disadvantages usually rule out routine use of chlorine solutions.
  • 49. Iodine Iodine is a faster disinfectant than a QAC or chlorine. The free iodine forms salts with the bacterial protein, thus killing the cell. Iodine is effective against vegetative bacteria, spores, fungi, and certain viruses. Iodophors make effective surface disinfectants and are easily prepared by mixing iodine concentrate with softened or distilled water (hard water and some concentrations of alcohol will inactivate the iodine). Other sources of disinfection include, but are not limited to, ultraviolet light, mercuric salts, hot oil, flaming, phenolic compounds, boiling water, and, more recently, microwaves.
  • 50. EFFECTS OF STERILIZATION & DISINFECTION ON ORTHODONTIC MATERIALS Orthodontic wires Smith and Von Fraunhofer in AJO 1992 studied the effect of clinical use and various sterilization/disinfection protocols on three types of nickel-titanium, and one type of β-titanium and stainless steel arch wire. The sterilization/disinfection procedures included,  Disinfection → with an iodophor for 10 minutes  Steam autoclave sterilization→ sterilization temperature of 274° F (134.4° C) for 10 minutes.
  • 51.  Cold sterilization→ freshly prepared sporocidin solution for 6.75 hours as per the manufacturer's recommendations.  Dry heat sterilization→ sterilization temperature of 375° F (191° C) was maintained for 10 minutes. The results indicated that load/deflection and tensile tests showed no clinically significant difference between asreceived and used-then-disinfected/sterilized wires and they concluded that nickel-titanium arch wires could be recycled at least once.
  • 52. Sunil Kapila, Haugen and Watanabe in AJO 1992 determined the effects of in vivo recycling interposed by dry heat sterilization (together referred to as clinical recycling, CR) on the load-deflection characteristics of nickel-titanium alloy wires (Nitinol and NiTi). The results indicated that both dry heat sterilization (DHS) alone, as well as clinical recycling (CR), produced significant changes in the loading and unloading characteristics of Nitinol and NiTi wires.
  • 53. However, the changes in the load-deflection characteristics of these wires after DHS only were relatively small, and the clinical significance of these changes is open to question. In contrast, the force levels during loading and unloading were substantially increased for both types of wires after CR. They concluded that, clinical recycling appears to reduce the "pseudoplasticity" and "pseudoelasticity" of NiTi wires and increases the stiffness of both NiTi and Nitinol wires.
  • 54. Mayhew and Kusy in AJO 1988 studied the effects of sterilization on the mechanical properties and the surface topography of 0.017 ×0.025-inch Nitinol and Titanal arch wires. Three approved heat sterilization methods were used namely,  Dry heat→ applied at 180° C (355° F) for 60 minutes  Formaldehyde alcohol vapor→ formaldehyde-alcohol vapor pressure of 20 to 25 psi for 30 minutes at 132° C (270° F)  Steam autoclave→ at 121° C (250° F) and 15 to 20 psi pressure for 20 minutes They concluded that neither the heat sterilization nor multiple cycling procedures had a deleterious effect on the elastic moduli, surface topography, or tensile properties of Nitinol or Titanal arch wires. The bending moduli and the tensile strengths were approximately 10% greater for Nitinol than for Titanal.
  • 55. Orthodontic pliers Vendrell and Hayden in AJO 2002 compared the wear of orthodontic ligature-cutting pliers after multiple cycles of cutting stainless steel ligature wire and sterilizing with dry heat or steam autoclave. Fifty ligature-cutting pliers with stainless steel inserts were randomly divided into 2 equal groups to be sterilized in either dry heat or steam autoclave.
  • 56. Each plier was subjected to a series of ligature wire cuts followed by the assigned sterilization method. The amount of wear at the tip of each plier in both groups was measured with a stereomicroscope system and digital photomicrography. Orthodontic ligature-cutting pliers with stainless steel inserts showed no significant difference in mean wear whether sterilized with steam autoclave or dry heat. Steam autoclave sterilization can be used with no significant deleterious effects on pliers with stainless steel inserts.
  • 57. Thank you For more details please visit www.indiandentalacademy.com