it's a protocol for Maximum Parsimony method using MEGA software...

1. Protocol
Very First step is open "Uniprot"
Then enter your Query in the dialog box.
e.g.:- "p53"
select more than 3 sequence for phylogenic tree.

2. here 10 sequence selected.
Then Retrieve for download sequence.
here take "fasta" format for download

3. Then open "MEGA"
in this open file > open a file/session
open fasta format file where you save....
then "align"

4. Select all sequence by ctrl+A
than from the menu bar select Alignment > Align by clastalW
Again from the menu bar select Data > Export Alignment > MEGA Format

5. than close the MEGA & open again.
from the menu bar select file > open a File/Session
 this time open that which you are save as mega format
not fasta format.
then select phylogeny > Construct / Test Maximum Parsimony
Tree(s)
So the phylogenic tree saw as follow:-