CURSO: Genómica aplicada a la Medicina Clínica BIOINFORMÁTICA Y NANOMEDICINA Barcelona 24/11/2010

1. Image Processing (I)
José Ramón Macías

2. Genome Replication

4. Transcription

6. Translation

8. Signal Transduction

10. Protein Folding

12. Viral Infection

14. Motility

16. Cellular Components

18. researchers use models from
structural studies to explain the
functions and mechanisms
of biological processes at the
molecular level

19. many key biological processes
are carried out by large
macromolecular complexes
acting as nanomachines

20. 15m
15x10-9m

22. Image Processing

23. 3D Electron
Microscopy

30. Top Side Bottom

31.      
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33. 91,114 particles (resolution: 9.9 Å)

34. 22,176 27,416 25,651 15,871
91,114 particles

35. 64 CPU’s : 3 days1 CPU: 6 months

37. Storage

38. E
M
D
B
lectron
icroscopy
ata
ank

43. 10 40 67
117
193
266
363
720
931
2002 2003 2004 2005 2006 2007 2008 2009 2010
since June 2002, the number
of structures has increased
steadily.
“
”

44. Single
Particle Icosahedral
Helical
Individual
Structure 2D Crystal
Today: 931 map
volume entries

46. Competitors

47. X-Ray
Crystallography
Requires
Crystals
High
Resolution 
3D Electron
Microscopy
Medium
Resolution 
Crystals
Not required
Hybrid Models
Fitting

48. 3D-EM model Atomic model

49. Hybrid model

50. Hybrid model
3D-EM model
Atomic model

52. Data Integration

62. http://biocomp.cnb.csic.es/das/PeppeR
DASx3DEM & PeppeR
VisualOmics