7. Figure 16.2
EXPERIMENT Mixture of
Heat-killed heat-killed
Living S cells Living R cells S cells S cells and
(control) (control) (control) living R cells
RESULTS
Mouse dies Mouse healthy Mouse healthy Mouse dies
Living S cells
19. Figure 16.7
G
5′ end
C
C G Hydrogen bond
3′ end
G C
G C T A
3.4 nm
T A
G C G C
C G
A T
1 nm C G
T A
C G
G C
C G A T
A T 3′ end
A T
0.34 nm
T A 5′ end
(a) Key features of (b) Partial chemical structure (c) Space-filling
DNA structure model
27. Figure 16.9-3
A T A T A T A T
C G C G C G C G
T A T A T A T A
A T A T A T A T
G C G C G C G C
(a) Parent molecule (b) Separation of (c) “Daughter” DNA molecules,
strands each consisting of one
parental strand and one
new strand
31. Figure 16.11 EXPERIMENT
1 Bacteria 2 Bacteria
cultured in transferred to
medium with medium with
15
N (heavy 14
N (lighter
isotope) isotope)
RESULTS
3 DNA sample 4 DNA sample Less
centrifuged centrifuged dense
after first after second
replication replication More
dense
CONCLUSION
Predictions: First replication Second replication
Conservative
model
Semiconservative
model
Dispersive
model
33. Figure 16.12
(a) Origin of replication in an E. coli cell (b) Origins of replication in a eukaryotic cell
Origin of Double-stranded
Parental (template) strand Origin of replication DNA molecule
replication
Daughter (new)
strand Parental (template) Daughter (new)
strand strand
Replication
Double- fork
stranded
DNA molecule Replication
bubble
Bubble Replication fork
Two daughter
DNA molecules
Two daughter DNA molecules
0.25 µm
0.5 µm
39. Figure 16.14
New strand Template strand
5′ 3′ 5′ 3′
Sugar A T A T
Phosphate Base
C G C G
G C G C
DNA
OH
polymerase
3′ A T A
T
P P Pi OH
P
P C Pyrophosphate 3′ C
OH
Nucleoside 2Pi
triphosphate 5′ 5′
41. Figure 16.15
Overview
Leading
Origin of replication Lagging
strand
strand
Primer
Lagging Leading
strand strand Origin of
Overall directions replication
of replication
3′
5′
5′ RNA primer
3′
3′ Sliding clamp
DNA pol III
Parental DNA 5′
3′
5′
5′
3′
3′
5′
43. Figure 16.16a
Overview
Leading Origin of replication Lagging
strand strand
Lagging strand
2
1
Leading
strand
Overall directions
of replication
44. LE 16-15_6
Primase joins RNA
nucleotides into a primer.
3′ 5′
5′ 3′
Template
strand DNA pol III adds
DNA nucleotides to
the primer, forming
an Okazaki fragment.
3′ 5′
RNA primer 3′
5′
After reaching the
next RNA primer (not
shown), DNA pol III Okazaki
falls off. fragment 3′
3′
5′
5′
After the second fragment is
primed, DNA pol III adds DNA
nucleotides until it reaches the
first primer and falls off.
5′ 3′
3′
5′
DNA pol I replaces
the RNA with DNA,
adding to the 3′ end
5′ of fragment 2. 3′
3′
5′
DNA ligase forms a
bond between the newest The lagging
DNA and the adjacent DNA strand in the region
of fragment 1. is now complete.
5′ 3′
3′ 5′
Overall direction of replication
45. Figure 16.17
Overview
Leading Origin of
replication Lagging
strand strand
Leading
Lagging strand
strand Overall directions
Leading strand of replication
5′ DNA pol III
3′ Primer
Primase
3′ 5′
3′
Parental DNA pol III Lagging strand
DNA 5′
4 DNA pol I DNA ligase
3′5′
3 2 1 3′
5′
47. Figure 16.18
DNA pol III
Parental DNA Leading strand
5′
5′ 3′ 3′
3′
3′ 5′ 5′
Connecting Helicase
protein
3′ 5′ Lagging
DNA strand
3′ Lagging strand template
pol III 5′
49. Figure 16.19
5′ 3′ A thymine dimer
distorts the DNA molecule.
3′ 5′
Nuclease
A nuclease enzyme cuts
the damaged DNA strand
5′ at two points and the
3′ damaged section is
removed.
3′ 5′
DNA
polymerase
Repair synthesis by
a DNA polymerase
5′ 3′ fills in the missing
nucleotides.
3′ 5′
DNA
ligase
DNA ligase seals the
5′ 3′ free end of the new DNA
to the old DNA, making the
strand complete.
3′ 5′
52. LE 16-18
5′
End of parental Leading strand
DNA strands Lagging strand
3′
Last fragment Previous fragment
RNA primer
Lagging strand 5′
3′
Primer removed but Removal of primers and
cannot be replaced replacement with DNA
with DNA because where a 3′ end is available
no 3′ end available
for DNA polymerase
5′
3′
Second round
of replication
5′
New leading strand 3′
New leading strand 5′
3′
Further rounds
of replication
Shorter and shorter
daughter molecules
58. Figure 16.22a
Nucleosome
(10 nm in diameter)
DNA double helix
(2 nm in diameter)
H1
Histone
Histones tail
Nucleosomes, or “beads on
DNA, the double helix Histones a string” (10-nm fiber)