2.  Complete the sequence of DNA of the human
genome

3.  There are 35,000 human genes in our genome

4.  The method at use in this project is called a
hierarchical shotgun
 There are 7 main steps to the method

5.  Break down into a code of 150,000 letters

6.  Each fragment was placed into a bacterial
artificial chromosome
 The E. coli inside the chromosome would
replicate each DNA sample

7.  Special enzymes were used to cut each clone
into ‘fingerprint’ of fragments

8.  Combined the clones into a longer strand
 Mapped them onto the human chromosome

9.  Broke down the cloned fragments into 1000-
2000 based pairs long
 Generated a sequence of the individual bases

10.  Shotgun method was used to sequence the
fragments of human DNA

11.  Overlaps of the letters of the genetic code were
used to assemble the sequence of DNA

12.  The first major experiment to map the human
genome is performed on a sheep named Dolly
 Deeper concepts were found in this experiment

13.  Map out the single gene that causes genetic
diseases or disorders

14.  Discoveries in the medical fields
 Cures of diseases

15.  Costs 3 billion dollars
 High risk in the project

16.  People will not got a chance to live there lives
fairly
 DNA is going to determine the opportunities

17.  Someday will be able to tell the life someone is
going to live by there human genome