2. Protein/Protein and Protein/Ligand interactions in large and dynamically disordered systems studied by NMR in solution Prof. K. Pervushin, BioNMR group , LPC, D-CHAB, ETH Zürich
5. Remodelling of outer membrane protein A A NMR conformer of the N-terminal domain of OmpA A BBP NMR structure exterior outer membrane periplasmic space EF-hand loop III Tb 3+ Thr Ser Asp Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Ala Ser
7. Role of flexible C-terminal 15 amino acids of 44 kDa BsCM in catalysis Endo -oxabicyclic transition state analog, TSA Putative transition state
8. Refolding of HTH DBP protein in 6 M Urea by Hofmeister reagents HTH in 6 M Urea unfolded HTH in 6 M Urea, 1.7 M NaCl native structure refolding HTH in 6 M Urea unfolded HTH in 6 M Urea, 0.5 M NaTFA distorted structure, molten globule refolding
14. Fundamental bounds associated with polarization/coherence transfer imposed by quantum spin dynamics C 1. Maximum transfer bound, U 2. Minimal spin-evolution time required for the transfer, min 3. Suppression of spurious transfers, Q 4. Combined use of more source operators, C
15. Definition of the optimization problem C U H = J I z S z + x (t) I x + y (t) I y
16. Definition of the optimization problem for isolated 2 spin ½ system S I DD( IS ), CSA( S ) and CSA( I ) interactions max (Khaneja et al, PNAS, 2003, 100, 13162)
17. Differential driving of the manifolds I and I by selective rf-pulse I z = I z + I z -> I z I z = 2 I z S z I i = I i (1/2 E + S z ) I i = I i (1/2 E S z ) I z I z
28. 1 H- 15 N RDCs measurements in the presence of Pf1 phages
29. Histogramm of RDCs values in two media C 12 E 5 / hexanol/H 2 O L n -Alkyl-poly(ethylene glycol)/ n -alkyl alcohol and glucopone/ n -hexanol mixtures Phages Pf1
37. Substrates recognized by GT RNase B RNase BS RNase B S protein alkylated RNase B - + - GT: RNase BS” S peptide 15-mer scrambled RNase B small glyco- peptides + - - -
38.
39. RNase A 15 N- 1 H HSQC RNase A: complete assignment is available
57. Thanx a lot! Alexander Eletski Prof. Donald Hilvert Beat Vögeli Prof. Linda Thöny-Meier Dr. Osvaldo Moreira Prof. Andreas Plückthun Kaifeng Hu Dr. Helena Kovac (Bruker AG) Alexander Kienhoffer Dr. Maria Johansson Simon Alioth Katherina Vamvaca Krystina Bromek Dr. Donghan Lee SNF and ETH for financial support Prof. Paul Barlow Prof. Ari Helenius Dr. Christiana Ritter