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HYBRIDIZATION
Hybridization The formation of a new organism
 by normal sexual processes or by protoplast
 fusion
Wide Hybridization (Interspecific
 Hybridization) Crosses made between distantly
 related species or genera
Somatic hybridization (Protoplast fusion)
 Crosses made between somatic cells
 One of the most effective methods of crops
 improvement programs
 Most the hybridization work carried out has
 used genetic variability within species
What is pollination?

• Pollination:
The transfer of pollen
from the male anther
to the female stigma
Why is pollination important?
•    Sexual reproduction is important for evolution
•    Sexual reproduction produces variable
    offspring, creating diversity and variation among
    populations (shuffling of genes)
•   You need variation for Natural Selection to occur
•    Sexual reproduction is advantageous to an
    organism only if it happens with someone other
    than itself!
•    Out-breeding = good! (inbreeding = bad…)
Sexual reproduction
• In animals: It’s easy because you have separate
  male and female individuals.
• In flowering plants: Not so easy, because most
  flowers have both male and female parts in
  them, called perfect flowers.
• So flowering plants have evolved special ways to
  insure out-breeding/out-crossing – and to
  prevent inbreeding.
Overview of floral organs
Reproductive floral organs:
 male

Stamen – male floral organ, consists of:
 Anther – part of the stamen that
 produces pollen
Filament –stalk like structure that holds
 anther
Pollen – immature male gametophyte
Reproductive floral organs: female
• Carpel or pistil – female reproductive organs;
  contains:
• Stigma – is where pollen sticks to
• Style – is the long tube that connects stigma to
  ovary
• Ovary – enlarged structure at the base of
  carpel/pistil where the ovules are located; it will
  become the fruit.
• Ovules – contains female gametophyte, becomes
  the seed
• Plants have style!
Non-reproductive floral organs
 Petals – whorl of flower organs
  that are often brightly colored
  to attract pollinators
 Corolla – whorl of petals in a
  flower
 Sepals – whorl of leaf-like
  organs outside the corolla;
  help protect the unopened
  flower bud.
 Calyx – whorl of sepals in a
  flower
 Tepals – when sepals and
  petals look the same
Pollination and Fertilization
• Pollen contains TWO nuclei: a sperm nucleus
  and tube nucleus
• Sperm nucleus is protected in gametophyte
  tissue (pollen can travel in the air)
Pollination and Fertilization
• For pollen sperm to successfully fertilize the egg,
  there must be pollination: a method to get the
  pollen from the male anther to the stigma.
• Pollen sticks to the stigma, starts growing a
  pollen tube
• Fertilization begins when tube begins to grow
  toward the egg
Double Fertilization
Double fertilization
  occurs: One sperm nucleus
  (1n) fertilizes the egg,
  producing a zygote (2n) 
  which becomes the plant
  embryo inside the seed
• Another sperm nucleus
  fuses with the polar nuclei,
  resulting in a triploid
  endosperm (3n)
• Endosperm is a source of
  food for the young embryo.
  Endosperm
Advantages

 More nutritious produce.
 Cheaper to grow.
 Larger flowers, fruits or vegetables.
 More adapted to new conditions.
Hybridization

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Hybridization

  • 2. Hybridization The formation of a new organism by normal sexual processes or by protoplast fusion Wide Hybridization (Interspecific Hybridization) Crosses made between distantly related species or genera Somatic hybridization (Protoplast fusion) Crosses made between somatic cells  One of the most effective methods of crops improvement programs  Most the hybridization work carried out has used genetic variability within species
  • 3. What is pollination? • Pollination: The transfer of pollen from the male anther to the female stigma
  • 4. Why is pollination important? • Sexual reproduction is important for evolution • Sexual reproduction produces variable offspring, creating diversity and variation among populations (shuffling of genes) • You need variation for Natural Selection to occur • Sexual reproduction is advantageous to an organism only if it happens with someone other than itself! • Out-breeding = good! (inbreeding = bad…)
  • 5. Sexual reproduction • In animals: It’s easy because you have separate male and female individuals. • In flowering plants: Not so easy, because most flowers have both male and female parts in them, called perfect flowers. • So flowering plants have evolved special ways to insure out-breeding/out-crossing – and to prevent inbreeding.
  • 7. Reproductive floral organs: male Stamen – male floral organ, consists of:  Anther – part of the stamen that produces pollen Filament –stalk like structure that holds anther Pollen – immature male gametophyte
  • 8. Reproductive floral organs: female • Carpel or pistil – female reproductive organs; contains: • Stigma – is where pollen sticks to • Style – is the long tube that connects stigma to ovary • Ovary – enlarged structure at the base of carpel/pistil where the ovules are located; it will become the fruit. • Ovules – contains female gametophyte, becomes the seed • Plants have style!
  • 9. Non-reproductive floral organs  Petals – whorl of flower organs that are often brightly colored to attract pollinators  Corolla – whorl of petals in a flower  Sepals – whorl of leaf-like organs outside the corolla; help protect the unopened flower bud.  Calyx – whorl of sepals in a flower  Tepals – when sepals and petals look the same
  • 10. Pollination and Fertilization • Pollen contains TWO nuclei: a sperm nucleus and tube nucleus • Sperm nucleus is protected in gametophyte tissue (pollen can travel in the air)
  • 11. Pollination and Fertilization • For pollen sperm to successfully fertilize the egg, there must be pollination: a method to get the pollen from the male anther to the stigma. • Pollen sticks to the stigma, starts growing a pollen tube • Fertilization begins when tube begins to grow toward the egg
  • 12. Double Fertilization Double fertilization occurs: One sperm nucleus (1n) fertilizes the egg, producing a zygote (2n)  which becomes the plant embryo inside the seed • Another sperm nucleus fuses with the polar nuclei, resulting in a triploid endosperm (3n) • Endosperm is a source of food for the young embryo. Endosperm
  • 13. Advantages More nutritious produce. Cheaper to grow. Larger flowers, fruits or vegetables. More adapted to new conditions.