2. Identify the tissue. Skeletal muscle. NOTE: the nuclei in each cell, characteristic cross striations, sarcomeres, endomysium that surrounds each muscle cell; differentiate between myofiber, myofibril, myofilament etc. Identify Z band, M line, A band, I band, H band etc.
3. Identify the tissue. Spinal cord – be able to identify endothelial cells, blood vessels, RBC’s (all in upper left corner), compare to larger ventral motor neurons if RBC’s are 7.5 um; know the function of ventral motor neurons. Identify motor neurons, neuropil, cell body, dendrites, recognize that this stain highlights cytoskeleton
4. Identify the tissue. Neural tissue EM 25,000x – identify the nucleus, nuclear pores, nuclear envelope, nucleolus, RER, mitochondria, golgi complex, Lysosome, polyribosome and cytoskeleton
11. Identify the tissue. Jejunum. Simple columnar epithelium, apical surface of the cells has microvilli (what is in the core of microvilli?), basement membrane, goblet cells (function?).
14. Identify the tissue. Skeletal muscle EM –Identify sarcomere, A band, H band, I band, Z line, M line, sarcoplasmic reticulum.
15. Identify the tissue. Thin skin 40x – name the 5 layers of thick skin…name the 4 layers of thin skin…identify in this slide stratum granulosum, stratum basale, basement membrane, dermis, epidermis, stratum spinosum, keratinized cells (note there aren’t any organelles, nucleus only protein keratin) stratum corneum.
18. Identify the tissue. Hypodermal and dermal tissue. Identify pacinian corpuscle and adipocytes
19. Identify the tissue. Kidney EM. This slide is mainly for understanding structure/function relationships of the organelles/cell. notice the plasma membrane infoldings of these cells, note the nuclei, mitochondria, plasma membrane, polyribosome and basement membrane
32. Identify the tissue. Trachea. Pseudostratified ciliated columnar epithelium PSCCE, cilia, basement membrane, connective tissue is in the lamina propria just below the epithelium…note that blood supply is in the lamina propria because epithelium is AVASCULAR…
34. Identify the tissue and what it’s showing. Skeletal Muscle and the difference between types of muscle fibers.
35. Identify the tissue. Skeletal muscle. Fibers/cells are circular in the cross sectionNuclei are located at the edge of the cell (periphery)Epimysium: surrounds large group of fascicles (bundles of fibers)Perimysium: CT in between each fascicleA -Endomysium: immediately around each fascicle B- Capillary C-Muscle fiber in cross section D- peripheral nucleus
37. Identify the tissue. Skeletal Muscle. A- Endomysium B-Sarcomere C-Nuclei D-Muscle fiber Light band = I-band; contains Z-disc (thin filaments)Dark band = A-line (thick filaments)
38. Identify the tissue. A- Myotendinous Junction B-Collagen C-Striated muscle D- Muscle Cell Nuclei E-Fibroblasts Myotendinous Junction. Tendon and muscle cells are attached to each other by collagen fibers of the tendon insert themselves into muscle fibers and associate w/ infoldings on sarcolemmaTendon made up of fibroblasts and type I collagen (dense regular CT)
39. Identify the tissue & structures. Skeletal Muscle. A-Muscle fibers B- nerve fibers C-motor end plate
45. Identify the tissue and what is occurring. Bone. Intramembranous Ossification O = osteoblasts laying down osteoid layerM = mesenchymal cells/tissuesB = boneV = vascularized areas
49. Identify the tissue and what is occurring. Bone. Endochondral Ossification A = Calcified Cartilage B = Osteoblasts laying down osteoidArrows = Osteocytes
50. Identify the tissue. Trachea again 10x – hyaline cartilage…because this isn’t articular cartilage it has a what??? (perichondrium located on the left side of the hyaline cartilage) identify chondrocytes, lacunae, isogenous groups, matrix…
54. Identify the tissue and what is occurring. Bone. Intramembranous Ossification A= resting zoneB = proliferative zoneC = hypertrophic cartilage zoneD = calcified cartilage zone E= ossification zone
68. Identify the tissue. Intestine EM – Identify RBC’s, nuclei, plasma membrane, pinocytic vesicles, ER, basement membrane.
69. Identify this tissue. Osteogenesis of Bone. Resting zone: cartilage cells get pushed down Proliferating zone: cells are active, dividing, forming stacks, and getting pushed down Hypertrophying zone: cells swell because of extra cytoplasm Calcification zone: matrix around cells gets calcified and cells begin to die Ossification zone: where bone tissue first starts to appear
70. Identify this tissue. Bone. Osteogenesis - Intramembranous ossification A – Osteoblasts B- Endothelial cell nucleus C – boney trabeculae
82. Identify these structures. Skin. A – epithelium B – small blood vessels C- fibroblasts D- Dense irregular CT E- Collagen bundles **note the lack of cells and high amount of ECM/collagen in the dense irregular connective tissue of the dermis. .
103. Identify this tissue. Elastic Cartilage. You can tell by special black stain that these are elastic fibers, there are still chondrocytes in lacunae present, isogenous groups are present, PERICHONDRIUM is present on both sides of the cartilage. .
105. Identify this tissue. TENDON. High power of the tendinous portion…this this dense regular connective tissue, note the fibroblasts and densely packed type I collagen fibers; he explains that the reason why this tendon is wavy looking only because it was on slack…so it shouldn’t normally look like this and I don’t think he would use this slide on the test .
106. Identify the structure. Synovial Joint. E - epiphyseal plate A – Articular hyaline cartilage SM – Synovial membrane
121. Identify this tissue. Loose Connective Tissue Note abundant ground substance, collagen fibers, elastic fibers, fibroblasts (most cells in this slide are fibroblasts) and macrophages (the one with the blue spots). Name a place where you would find LCT and what is it’s function? (dermis, supports vascular structures, mucous membranes, mesenteries)
124. Identify this tissue. This is from thymus tissue (20x) – note the adipocytes, capillaries running in between the adipocytes, because this is thymus all of the purple spots are white blood cells (probably T cells) The two large vessels on the left are arteries and veins…identify tunica intima, media and adventitia. What types of tissue lines the lumen of the blood vessels? (simple squamous epithelium/endothelium) Also what type of collagen are reticular fibers? (type III) See fig 5.46 in our book for a better pic of reticular tissue.
127. Identify this organ. Skin. What are the different layers? What layer is the lighter pink? What type of tissue is this? What type of collagen is found in the light pink layer? (Thick skin, epidermis and dermis, dermis, dense irregular connective tissue, type I collagen) .
128. Identify this tissue and structure labeled “?” Cardiac Muscle with Purkinje cells in top left corner.
129. Identify this organ. Skin. Hypodermis – note the large amount of adipose. The darker pink at the top of the slide is dense reg connective tissue; sweat glands are in the center of the slide. .
130. Identify this tissue. Elastic Cartilage. A – chondrocyte nuclei B – elastin fibers .
131. Identify this tissue. Tendon! These are dense regular connective tissue…made of type I collagen, **note that there is more ecm than cells…compare to smooth muscle slides…so think what is the function of tendon in relationship to muscle and bone? How is the organization of a tendon different from a ligamnet? There are other figures in our book of longitudinal and cross section of tendon .
133. Identify this tissue. Muscle. What would the lighter pink at the bottom be? epimysium, perimysium, endomysium? What type of connective tissue? What type of collagen fibers? ( I think this is perimysium (but it could be epimysium too), because it surrounds muscle fibers as a fascicle and there are blood vessels in the connective tissue, dense irregular connective tissue, and I think it’s type I collagen) A – Muscle cell nuclei B – muscle fiber C – fibroblasts D – blood vessel .
136. Arrow pointing to the joint space Letter A is type A cells (macrophage like cells)of synovial membrane, letter B is type B cells (fibroblast like cells) of the synovial membrane that are closely associated capillaries
137. Identify this condition. Healing. Left side: perfectly parallel collagen fibers Right side: healing collagen fibers before they have organized into parallel fibers
138. Identify the tissue. Human Fibular Fracture Site Fracture site Hyaline cartilage Formation of new bone
139. Note the pathology on the right slide. Fissures in articular surfaces Notice how the surface is very uneven, should be nice and smooth!
141. Identify tissue type and structures. Nervous Tissue. A –fibroblast B –Schwann's cell nucleus C –Endoneurium D- Myelin sheath E – axons F – node of Ranvier
142. Identify the structures. D. Peritubular capillaries E. Glomeruli F. Interlobar capillaries Glomerular capillaries Vascular pole Macula densa
147. Identify the tissue undergoing healing. Bone. A -Necrotic bone: no cells inside of the lacunae B- Granulation tissue: BV’s and fibroblasts C - Chondrocytes making hyaline cartilage template D- Fracture Callus
148. Identify tissue type and structures. Nervous Tissue. A – Satellite Cells B – Axons C – Nissl Substance D- Neuronal Cell body E- Basal Lamina F – endothelial cell nucleus G - fibroblasts
149. Identify the 3 layers and their content. A-Fibrous Tunic B- Sclera C-Cornea D- Vascular Tunic E- iris F- Ciliary body G- choroid H- Retina I- pigmented layer J- neural layer
150. Identify blanks and overall structure. Lens. A – Lens Epithelium B- Lens Capsule C- Differentiating lens fibers D – Mature Lens fibers
156. Identify this condition. Effect of DOMS (bottom picture) -disruption of parallel A bands and I bands -z-discs are disrupted
157. Identify this tissue. Granulation Tissue. Left: granulation tissue- In growth of blood vessels Right: granulation tissue- not as many blood vessels here, collagen from fibroblasts
158. Identify the tissue. Granulation tissue Bone Fibrous tissue trying to bridge fracture site
159. Identify tissue type and structures. Nervous Tissue. A – axon B – schwann cell nucleus C – node of Ranvier D- myelin sheath E – fibroblasts.
160. Identify the structures. A. Granulation tissue D. Granulation tissue B. Osteoblasts E. Necrotic bone (dead bone): empty lacunae C. Osteoclasts F. osteoblasts
161. Identify tissue type and structures. Nervous Tissue. A –Epineurium B –Perineurium C –nerve fascicle D- blood vessel
162. Identify blanks and overall structure. Cochlear Duct A- Spiral Ganglion B- Scala Tympani B C-periosteum D-scala vestibuli E- Spiral Organ F-Stria Vascularis G-Cochlear duct (scala media)
163. Identify all structures & overall tissue. Nervous Tissue. A – Dendrite B – Nucleolus C- Alpha motor neuron D - Oligodendrocyte E – Astrocyte
164. Identify the pathology and tissue of bottom slide. Osteoarthritis Cartilage Fissures Clustered chondrocytes
165. Identify the structures. Podocyte E. Lumen of Basal Lamina Small black line= diaphragm F. Endothelial Cell of capillary Basal lamina G. Filtration Slit Fenestration H. Pedicle
166. Identify the tissue undergoing healing. Bone. A- showing trabecular bone B - showing cortical bone The blue stained area is the fracture site healing, hard callus stage because there is a bridge almost entirely through fracture site, near radiographic union
169. Identify blanks and overall structure. Cornea. A – stratified squamous epithelium B- stroma C- simple squamous endothelium D – bowman’s membrane E – Decement’s membrane F –under epithelium G – under endothelium
170. Identify this condition. Inflammation. A. WBC’s are sticking to the wall of dilated blood vessels B. WBC’s are migrating C .WBC’s are emigrating to the area of injury forming EXUDATE
171. Identify blanks and overall structure. Iris. A – Dilator Pupillae muscles B- Sphincter Pupillae C – Pigmented epithelium Arrows – blood vessels
172. Identify tissue type and structures. Nervous Tissue. A –endoneurium B –Perineurium C –nerve fibers D- Myelin sheath E - axons