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  the evaluation of the nutritional status of individuals or
   populations through anthropometry, biochemical, clinical
   and dietary measurements.
 the measurement of indicators of dietary status and nutrition-
   related health status to determine the possible
   occurrence, nature and extent of impaired nutritional status
   which can range from deficiency to toxicity (US Department
   of Health and Human Services).
Types or Forms of Nutritional Assessment Systems
1. Nutritional survey - an epidemiological investigation of the
    nutritional status of a population by various methods; may
    include an evaluation of factors affecting nutritional status.
 useful in establishing baseline nutritional status and/or
    ascertaining the overall nutritional status of the population;
 if cross-sectional, can identify and define those population
    sub-groups at risk of chronic malnutrition.
    less likely to identify acute malnutrition
    if socio-economic, ecologic and demographic information
     are simultaneously collected, possible causes of malnutrition
     may be identified through statistical analysis of data.

2.   Nutritional surveillance - continuous monitoring of the
     nutritional status selected population groups.
    unlike surveys, data are collected, analyzed and utilized for
     an extended period of time.
    useful in identifying causes of malnutrition, hence can be
     used in formulating and initiating intervention measures.

3.   Nutrition screening - involves comparing an individual’s
     measurements with predetermined risk levels or “cut-off”
     points.
•    usually less comprehensive than survey or surveillance;
•    useful in identifying individuals in need of immediate
     intervention. Operation Timbang collects only age and
     weight data, targets only preschoolers, and is used to screen
     children for inclusion in food assistance programs.
Purposes of Nutritional Assessment
1.  Define nutritional problems that need attention; as an integral
    part of situational analysis, it is the first step in the nutrition
    program planning and management cycle.
2.  Provide baseline data for planning and evaluation of programs.
3.  Help identify priorities and responsibilities of the public health
    system at all administrative levels (i.e. from national to barangay
    level).
Methods of Nutritional Assessment
1.  Methods that provide direct information on nutritional status
a) clinical examination
b) biochemical examination
c) anthropometry
d) biophysical methods (e.g. measures of body composition, bone
    density)

2.   Methods that provide indirect information
a)   food consumption studies
b)   studies on health conditions and vital statistics (special on infant
     and child mortality rates)
c)   studies on the food supply situation
d)    studies on socio-economic conditions
e)    studies on cultural and anthropological influences
     Factors Affecting Choice of Nutritional Assessment System
      and Method
1.    Objectives of nutritional assessment, e.g.
•     to define current overall nutritional status, a nutrition survey
      using clinical, biochemical, anthropometric and dietary
      (food consumption) methods is essential.
•     to evaluate the impact of nutrition intervention, a monitoring
      system is used and the choice of method depends on the
      objective of the intervention, e.g.,
       - anthropometric methods for feeding programs;
       - clinical or biochemical methods for nutrient
      supplementation programs.
•     to identify malnourished or individuals needing immediate
      intervention, a screening system using indices of past and
      present nutrition must be used.

2.   Unit to be assessed, e.g. household, individuals, population
     groups
•    biochemical methods may not be feasible for household
     level assessment.
3.   Type of information required for program planning and
     evaluation purposes, e.g.
•    for nutrition education, food consumption data
4.   Degree of reliability and accuracy required – usually requires
     a combination of at least two methods
     (clinical, biochemical, anthropometric, dietary
     methods), preferably all four.
5.   Facilities and equipment available. Biochemical and
     biophysical methods require facilities and equipment which
     may not be readily available.
6.   Manpower resources and training required, e.g.
•    clinical methods require a medical nutritionist trained in the
     detection of deficiency signs and symptoms;
•    biochemical methods require a biochemist, chemist or
     medical technologist;
•    anthropometric methods require trained technicians;
•    dietary methods require nutritionist-dietitians trained in food
     consumption data collection and analysis methods.
7.   Time reference: season of the year, week-end, week
     day, numbers of days of data collection.
8.   Funding and financial support available.

CLINICAL ASSESSMENT
A. Description : deals with the examination of changes that can
   be seen or felt in superficial tissues, such as
   skin, eyes, hair, etc.
B. Advantages
 more coverage in a short time
   inexpensive, no need for sophisticated equipment
C. Disadvantages
1. non-specificity of signs (signs may be due to non-nutritional
   causes)
2. Overlapping of deficiency states (dietary deficiencies are
   not restricted to an isolated nutrient)
3. Bias of the observer (observations of two examiners are most
   often not consistent with each other)
Clinical Signs of Value in Nutrition Assessment and Their
                          Interpretation
Tissue/body part    Signs                     Associated Disorder or
                                              Nutrient
1. Hair             Lack of lustre            Kwashiorkor, less
                    Thinness and sparseness   commonly, marasmus
                    Straightness
                    Dyspigmentation
                    Flag sign
                    Easy pluckability

                    naso-labial dyssebaccea
2. Face             Moon-face                 Riboflavin
                                              Kwashiorkor
                    Pale conjunctiva          Anemia (iron etc.)
                    Bitot’s spots
3. Eyes             Conjunctival xeroxis
                    Corneal xeroxis
                    Keratomalacia             Vitamin A
                    Angular palpebritis

                    Angular stomatitis
                    Angular scars
4. Lips             Cheilosis                 Riboflavin
Predominant Clinical Symptoms of Common Nutritional Problems
1. Protein-energy malnutrition
a)  Mild to moderate – low weight and/or height for age
b)  Severe (marasmus and kwashiorkor)

2.   Xerophthalmia – affects the eyes, gradually beginning with
     an impairment of night vision. Symptoms include:
a)   Night blindness
b)   Cornea softening and ulceration
c)   Skin changes are usually non-specific

3. Anemia – clinical symptoms are non-specific (may be due to
    other conditions) and should be confirmed with biochemical
    test, e.g. for blood hemoglobin level. Symptoms include:
a) Paleness under the eyelids
b) Paleness under the nails
4. Beriberi – symptoms include:
a) Muscle weakness, fatigability
b) Heart enlargement, tachycardia, edema (in wet type)


5.   Goiter – symptoms include:
a)   Swelling of the neck
b)   Lassitude and easy fatigability

6.   Ariboflavinosis – symptoms are non-specific and may
     include:
a)   Magenta red tongue
b)   Sores at the angles of the mouth and folds of the nose.

 Interpretations Guides
1. WHO Criteria for determining whether a significant public
     health problem of xerophthalmia and vitamin A deficiency
     exists in a population
   Night blindness (XN) – greater than 1%
   Bitot’s spots (XIB) – greater than 0.5%
   Corneal xeroxis/corneal ulceration/keratomalacia
    (X2/X3A/X3B) – greater than 0.05%
   Plasma vitamin A of less than 10 ug/dl – greater than 5%
Biochemical Assessment
Description: estimation of tissue desaturation, enzyme activity or
   blood composition.
1. Tests are confined to two fairly easily obtainable fluids; blood
    and urine.
2. Results are generally compared to standards, i.e., normal
    levels for age and sex.
Advantages
1. objectivity, i.e., independent of the emotional and
    subjective factors than usually affect the investigator.
2. can detect early subclinical states of nutritional deficiency
    (i.e., before clinical symptoms appear).
Disadvantages
1. costly, usually requiring expensive equipments
2. time consuming
3. difficulty in collecting samples
4. lack of practical standards of sample collection
Factors Affecting Accuracy of Results
1. method of sample collection
2. method of transport and storage of samples
3. techniques employed
Biochemical Measurements Which May be Done in Nutritional
    Status Surveys
1. Protein status
a. Urea nitrogen/creatine nitrogen ratio – determined from a 3
    to 4 hour or 24 hours urine sample
•   A ratio of 30 or lower is indicative of malnutrition
b. Amino acid imbalance test – the ratio of four dispensable
    amino acids and four indispensable amino acid is
    determined by paper chromatography.
•   A high ratio of 5-10 is indicative of kwashiorkor.
•   The ratio is low (less than 2) in well-fed children.
c. Hydroxyproline excretion in random urine sample.
•   Low (0.5 – 1.5) in clinically malnourished
•   Normal level: 2.0 – 2.5
d.    Serum albumin – most common biochemical test for protein nutriture.
•     Guide to interpretation (g/100ml):
     - High: 4.25
     - Acceptable: 3.52 – 4.24
     - Low: 2.80 – 3.51
     - Deticient: less than 2.80
2.    Protein – Energy status:

3.   Vitamin A status
a.   Serum vitamin A
•    Guide to interpretation: a serum level of 10-20 ug/dl is considered low,
     while <10 ug/dl is considered deficient.
•    A prevalence rate of 10% for “deficient” serum levels and 15% of “low”
     serum levels indicate the existence of a public health problem in the
     community.
b.   Serum carotene
•    Guide to interpretation. A serum level of equal or less than 39 ug/dl is
     considered low.
•    Low serum carotene levels per se are not indicative of vitamin A
     deficiency but reflect current intake of carotene which is a precursor of
     the vitamin.
4.   Thiamine status
a.   Urinary thiamine – less preferred test.
b.   Erythrocyte transketolase activity (ETKA) with and without
     addition of thiamine triphosphate (TPP) in vitro.
c.   Blood pyruvate level – increased I thiamine deficiency.

5.   Riboflavin status
a.   Urinary riboflavin – less preferred test
b.   Erythrocyte glutathione reductase activity coefficient (EGR-
     AC)
•    Guide to interpretation: normal EGR-AC value is 1.0 – 1.3;
     higher values indicate riboflavin deficiency.

6.   Ascorbic acid status
a.   Serum ascorbic acid
•    Interpretation guide: a serum ascorbic acid level of 0.8
     mg/dl is considered “acceptable” or “ good”. Lower levels
     indicate ascorbic acid deficiency.
7.   Iron status
a.   Hemoglobin
•    Values below which anemia is said to exist
      - infants and children, 6 mos. To 6 years: 11 grams %
      - children and adolescents, 6 years to 14 years: 12 grams %
      - adult males: 12 grams %
      - adult females, non-pregnant: 12 grams %
      - adult females, pregnant: 11 grams %
b.   Hematocrit
•    Normal values
      - females: 37-47%
      - males: 45-52%
c.   Total iron binding capacity (TIBC)
•    Normal value: 250-425 mg/dl
d.   Transferrin saturation
•    Normal value: 20-50%
e.   Ferritin
•    Normal level: 30-250 mg/dl
Iodine status
a. Urinaru iodine
•   Guide to interpretation – epidemiological criteria for
    assessing severity of IDD based on median urinary iodine
    levels.
Median ug/L
ANTHROPOMETRY
 The measurement of variations of physical dimensions and
   gross composition of the human body at different age levels
   and degrees of nutrition.
Common Anthropometric Measurements
1. Weight (for age)
 Uses weighing scales such as beam balance scales or
   clinical scales which are ideal, but a bar scale could be used
   in their absence.
 Assesses body mass; an indicator of current nutritional status
   of preschoolers.
 Advantages:

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Public health nutrition

  • 1.
  • 2.  the evaluation of the nutritional status of individuals or populations through anthropometry, biochemical, clinical and dietary measurements.  the measurement of indicators of dietary status and nutrition- related health status to determine the possible occurrence, nature and extent of impaired nutritional status which can range from deficiency to toxicity (US Department of Health and Human Services). Types or Forms of Nutritional Assessment Systems 1. Nutritional survey - an epidemiological investigation of the nutritional status of a population by various methods; may include an evaluation of factors affecting nutritional status.  useful in establishing baseline nutritional status and/or ascertaining the overall nutritional status of the population;  if cross-sectional, can identify and define those population sub-groups at risk of chronic malnutrition.
  • 3. less likely to identify acute malnutrition  if socio-economic, ecologic and demographic information are simultaneously collected, possible causes of malnutrition may be identified through statistical analysis of data. 2. Nutritional surveillance - continuous monitoring of the nutritional status selected population groups.  unlike surveys, data are collected, analyzed and utilized for an extended period of time.  useful in identifying causes of malnutrition, hence can be used in formulating and initiating intervention measures. 3. Nutrition screening - involves comparing an individual’s measurements with predetermined risk levels or “cut-off” points. • usually less comprehensive than survey or surveillance; • useful in identifying individuals in need of immediate intervention. Operation Timbang collects only age and weight data, targets only preschoolers, and is used to screen children for inclusion in food assistance programs.
  • 4. Purposes of Nutritional Assessment 1. Define nutritional problems that need attention; as an integral part of situational analysis, it is the first step in the nutrition program planning and management cycle. 2. Provide baseline data for planning and evaluation of programs. 3. Help identify priorities and responsibilities of the public health system at all administrative levels (i.e. from national to barangay level). Methods of Nutritional Assessment 1. Methods that provide direct information on nutritional status a) clinical examination b) biochemical examination c) anthropometry d) biophysical methods (e.g. measures of body composition, bone density) 2. Methods that provide indirect information a) food consumption studies b) studies on health conditions and vital statistics (special on infant and child mortality rates) c) studies on the food supply situation
  • 5. d) studies on socio-economic conditions e) studies on cultural and anthropological influences Factors Affecting Choice of Nutritional Assessment System and Method 1. Objectives of nutritional assessment, e.g. • to define current overall nutritional status, a nutrition survey using clinical, biochemical, anthropometric and dietary (food consumption) methods is essential. • to evaluate the impact of nutrition intervention, a monitoring system is used and the choice of method depends on the objective of the intervention, e.g., - anthropometric methods for feeding programs; - clinical or biochemical methods for nutrient supplementation programs. • to identify malnourished or individuals needing immediate intervention, a screening system using indices of past and present nutrition must be used. 2. Unit to be assessed, e.g. household, individuals, population groups • biochemical methods may not be feasible for household level assessment.
  • 6. 3. Type of information required for program planning and evaluation purposes, e.g. • for nutrition education, food consumption data 4. Degree of reliability and accuracy required – usually requires a combination of at least two methods (clinical, biochemical, anthropometric, dietary methods), preferably all four. 5. Facilities and equipment available. Biochemical and biophysical methods require facilities and equipment which may not be readily available. 6. Manpower resources and training required, e.g. • clinical methods require a medical nutritionist trained in the detection of deficiency signs and symptoms; • biochemical methods require a biochemist, chemist or medical technologist; • anthropometric methods require trained technicians; • dietary methods require nutritionist-dietitians trained in food consumption data collection and analysis methods.
  • 7. 7. Time reference: season of the year, week-end, week day, numbers of days of data collection. 8. Funding and financial support available. CLINICAL ASSESSMENT A. Description : deals with the examination of changes that can be seen or felt in superficial tissues, such as skin, eyes, hair, etc. B. Advantages  more coverage in a short time  inexpensive, no need for sophisticated equipment C. Disadvantages 1. non-specificity of signs (signs may be due to non-nutritional causes) 2. Overlapping of deficiency states (dietary deficiencies are not restricted to an isolated nutrient) 3. Bias of the observer (observations of two examiners are most often not consistent with each other)
  • 8. Clinical Signs of Value in Nutrition Assessment and Their Interpretation Tissue/body part Signs Associated Disorder or Nutrient 1. Hair Lack of lustre Kwashiorkor, less Thinness and sparseness commonly, marasmus Straightness Dyspigmentation Flag sign Easy pluckability naso-labial dyssebaccea 2. Face Moon-face Riboflavin Kwashiorkor Pale conjunctiva Anemia (iron etc.) Bitot’s spots 3. Eyes Conjunctival xeroxis Corneal xeroxis Keratomalacia Vitamin A Angular palpebritis Angular stomatitis Angular scars 4. Lips Cheilosis Riboflavin
  • 9.
  • 10.
  • 11.
  • 12. Predominant Clinical Symptoms of Common Nutritional Problems 1. Protein-energy malnutrition a) Mild to moderate – low weight and/or height for age b) Severe (marasmus and kwashiorkor) 2. Xerophthalmia – affects the eyes, gradually beginning with an impairment of night vision. Symptoms include: a) Night blindness b) Cornea softening and ulceration c) Skin changes are usually non-specific 3. Anemia – clinical symptoms are non-specific (may be due to other conditions) and should be confirmed with biochemical test, e.g. for blood hemoglobin level. Symptoms include: a) Paleness under the eyelids b) Paleness under the nails
  • 13. 4. Beriberi – symptoms include: a) Muscle weakness, fatigability b) Heart enlargement, tachycardia, edema (in wet type) 5. Goiter – symptoms include: a) Swelling of the neck b) Lassitude and easy fatigability 6. Ariboflavinosis – symptoms are non-specific and may include: a) Magenta red tongue b) Sores at the angles of the mouth and folds of the nose. Interpretations Guides 1. WHO Criteria for determining whether a significant public health problem of xerophthalmia and vitamin A deficiency exists in a population
  • 14. Night blindness (XN) – greater than 1%  Bitot’s spots (XIB) – greater than 0.5%  Corneal xeroxis/corneal ulceration/keratomalacia (X2/X3A/X3B) – greater than 0.05%  Plasma vitamin A of less than 10 ug/dl – greater than 5%
  • 15.
  • 16. Biochemical Assessment Description: estimation of tissue desaturation, enzyme activity or blood composition. 1. Tests are confined to two fairly easily obtainable fluids; blood and urine. 2. Results are generally compared to standards, i.e., normal levels for age and sex. Advantages 1. objectivity, i.e., independent of the emotional and subjective factors than usually affect the investigator. 2. can detect early subclinical states of nutritional deficiency (i.e., before clinical symptoms appear). Disadvantages 1. costly, usually requiring expensive equipments 2. time consuming 3. difficulty in collecting samples 4. lack of practical standards of sample collection
  • 17. Factors Affecting Accuracy of Results 1. method of sample collection 2. method of transport and storage of samples 3. techniques employed Biochemical Measurements Which May be Done in Nutritional Status Surveys 1. Protein status a. Urea nitrogen/creatine nitrogen ratio – determined from a 3 to 4 hour or 24 hours urine sample • A ratio of 30 or lower is indicative of malnutrition b. Amino acid imbalance test – the ratio of four dispensable amino acids and four indispensable amino acid is determined by paper chromatography. • A high ratio of 5-10 is indicative of kwashiorkor. • The ratio is low (less than 2) in well-fed children. c. Hydroxyproline excretion in random urine sample. • Low (0.5 – 1.5) in clinically malnourished • Normal level: 2.0 – 2.5
  • 18. d. Serum albumin – most common biochemical test for protein nutriture. • Guide to interpretation (g/100ml): - High: 4.25 - Acceptable: 3.52 – 4.24 - Low: 2.80 – 3.51 - Deticient: less than 2.80 2. Protein – Energy status: 3. Vitamin A status a. Serum vitamin A • Guide to interpretation: a serum level of 10-20 ug/dl is considered low, while <10 ug/dl is considered deficient. • A prevalence rate of 10% for “deficient” serum levels and 15% of “low” serum levels indicate the existence of a public health problem in the community. b. Serum carotene • Guide to interpretation. A serum level of equal or less than 39 ug/dl is considered low. • Low serum carotene levels per se are not indicative of vitamin A deficiency but reflect current intake of carotene which is a precursor of the vitamin.
  • 19. 4. Thiamine status a. Urinary thiamine – less preferred test. b. Erythrocyte transketolase activity (ETKA) with and without addition of thiamine triphosphate (TPP) in vitro. c. Blood pyruvate level – increased I thiamine deficiency. 5. Riboflavin status a. Urinary riboflavin – less preferred test b. Erythrocyte glutathione reductase activity coefficient (EGR- AC) • Guide to interpretation: normal EGR-AC value is 1.0 – 1.3; higher values indicate riboflavin deficiency. 6. Ascorbic acid status a. Serum ascorbic acid • Interpretation guide: a serum ascorbic acid level of 0.8 mg/dl is considered “acceptable” or “ good”. Lower levels indicate ascorbic acid deficiency.
  • 20. 7. Iron status a. Hemoglobin • Values below which anemia is said to exist - infants and children, 6 mos. To 6 years: 11 grams % - children and adolescents, 6 years to 14 years: 12 grams % - adult males: 12 grams % - adult females, non-pregnant: 12 grams % - adult females, pregnant: 11 grams % b. Hematocrit • Normal values - females: 37-47% - males: 45-52% c. Total iron binding capacity (TIBC) • Normal value: 250-425 mg/dl d. Transferrin saturation • Normal value: 20-50% e. Ferritin • Normal level: 30-250 mg/dl
  • 21. Iodine status a. Urinaru iodine • Guide to interpretation – epidemiological criteria for assessing severity of IDD based on median urinary iodine levels.
  • 23. ANTHROPOMETRY  The measurement of variations of physical dimensions and gross composition of the human body at different age levels and degrees of nutrition. Common Anthropometric Measurements 1. Weight (for age)  Uses weighing scales such as beam balance scales or clinical scales which are ideal, but a bar scale could be used in their absence.  Assesses body mass; an indicator of current nutritional status of preschoolers.  Advantages: